Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 134,028 G→T 100% A153A (GCG→GCT)  cueO → multicopper oxidase (laccase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273134,0280GT97.3% 836.1 / 8.6 280A153A (GCG→GCT) cueOmulticopper oxidase (laccase)
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (140/132);  total (144/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 280 total aligned reads displayed.

GCTGGAAGTACCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCGATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGA  >  CP009273/133881‑134171
                                                                                                                                                   |                                                                                                                                               
  tGGAAGTACCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATgg                                                                                                                                             <  2:2985451/150‑1 (MQ=255)
      aGTACCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGggct                                                                                                                                         <  1:1571925/150‑1 (MQ=255)
        tACCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGggctgg                                                                                                                                       >  1:3054242/1‑150 (MQ=255)
         aCCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGggctggc                                                                                                                                      <  1:5009956/150‑1 (MQ=255)
         aCCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGggctggc                                                                                                                                      <  2:4198584/150‑1 (MQ=255)
         aCCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGggctggc                                                                                                                                      >  2:1498873/1‑150 (MQ=255)
          ccGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGggctggct                                                                                                                                     <  1:2732331/150‑1 (MQ=255)
          ccGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGggctggct                                                                                                                                     >  1:703922/1‑150 (MQ=255)
            gggTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGctggctgg                                                                                                                                   >  2:2585933/1‑150 (MQ=255)
             ggTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTggctggg                                                                                                                                  >  2:3708806/1‑150 (MQ=255)
              gTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTggctgggc                                                                                                                                 <  2:4420886/150‑1 (MQ=255)
                     cGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTggtggt                                                                                                                          >  1:4201143/1‑150 (MQ=255)
                      gACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGgtggtg                                                                                                                         <  1:2267178/150‑1 (MQ=255)
                         ggcggcCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGAtt                                                                                                                      <  1:2611134/150‑1 (MQ=255)
                          gcggcCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTg                                                                                                                     >  1:1862530/1‑150 (MQ=255)
                            ggcCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGaa                                                                                                                   <  2:2459690/150‑1 (MQ=255)
                            ggcCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGaa                                                                                                                   <  2:4024187/150‑1 (MQ=255)
                             gcCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAg                                                                                                                  <  2:2362946/150‑1 (MQ=255)
                              cccGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGa                                                                                                                 >  2:1995756/1‑150 (MQ=255)
                              cccGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGa                                                                                                                 >  2:3564988/1‑150 (MQ=255)
                                      gAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACgaga                                                                                                         <  2:4346565/150‑1 (MQ=255)
                                       aaTTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGAt                                                                                                        <  2:5001766/150‑1 (MQ=255)
                                        attattCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATc                                                                                                       >  2:2701856/1‑150 (MQ=255)
                                        attattCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATc                                                                                                       >  2:100820/1‑150 (MQ=255)
                                          tattCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCt                                                                                                     <  1:4458571/150‑1 (MQ=255)
                                          tattCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCGATGGGGCTGGCTGGGCGGGTGGTGATTGAAGATGACGAGATCCt                                                                                                     >  1:449063/1‑150 (MQ=255)
                                          tattCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACAGGGAGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCt                                                                                                     >  1:4069642/1‑150 (MQ=255)
                                           attCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTg                                                                                                    >  1:511341/1‑150 (MQ=255)
                                            ttCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGa                                                                                                   >  1:616689/1‑150 (MQ=255)
                                             tCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGaa                                                                                                  <  2:770365/150‑1 (MQ=255)
                                              ccGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGaaa                                                                                                 <  1:1780850/150‑1 (MQ=255)
                                               cGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAAt                                                                                                <  2:3841463/150‑1 (MQ=255)
                                                    ggTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAAtg                                                                                           <  1:3329171/150‑1 (MQ=255)
                                                    ggTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAAtg                                                                                           <  2:1679887/150‑1 (MQ=255)
                                                     gTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAAtgc                                                                                          <  1:4319864/150‑1 (MQ=255)
                                                      tGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAAtgct                                                                                         >  1:4895423/1‑150 (MQ=255)
                                                        gCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAAtgctgc                                                                                       <  1:1947471/150‑1 (MQ=255)
                                                        gCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAAtgctgc                                                                                       <  2:2073967/150‑1 (MQ=255)
                                                           aGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCaa                                                                                    <  2:671599/150‑1 (MQ=255)
                                                            gcgcTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCaaa                                                                                   >  2:4463030/1‑150 (MQ=255)
                                                            gcgcTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCaaa                                                                                   >  2:129297/1‑150 (MQ=255)
                                                                tCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAg                                                                               <  1:4444304/150‑1 (MQ=255)
                                                                tCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAg                                                                               <  2:2725692/150‑1 (MQ=255)
                                                                 cGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGt                                                                              <  2:1390467/150‑1 (MQ=255)
                                                                  ggTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTg                                                                             >  1:3231059/1‑150 (MQ=255)
                                                                    tGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTggg                                                                           >  2:3136309/1‑150 (MQ=255)
                                                                    tGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGGGGCTATGGGGCTGGCGGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTggg                                                                           >  2:3351567/1‑150 (MQ=255)
                                                                        gttgaacgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTAt                                                                       >  2:5109065/1‑150 (MQ=255)
                                                                        gttgaacgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTAt                                                                       >  2:3270132/1‑150 (MQ=255)
                                                                          tgaacgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCg                                                                     >  1:4363811/1‑150 (MQ=255)
                                                                          tgaacgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCg                                                                     >  1:1726207/1‑150 (MQ=255)
                                                                          tgaacgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCg                                                                     >  1:1446395/1‑150 (MQ=255)
                                                                           gaacgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCga                                                                    <  2:4186871/150‑1 (MQ=255)
                                                                            aacgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCgat                                                                   <  1:3152971/150‑1 (MQ=255)
                                                                             acgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCgatg                                                                  <  1:2454083/150‑1 (MQ=255)
                                                                              cgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCgatga                                                                 >  2:808125/1‑150 (MQ=255)
                                                                              cgttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCgatga                                                                 >  2:3501218/1‑150 (MQ=255)
                                                                               gttgaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCgatgat                                                                >  2:2223182/1‑150 (MQ=255)
                                                                                  gaTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGtt                                                             >  1:1657375/1‑150 (MQ=255)
                                                                                   aTCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGACATTAATGCTGCCAAAACAGTGGGGTATCGAGGCTGTTc                                                            >  2:4801969/1‑150 (MQ=255)
                                                                                     cAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCg                                                          <  2:3788824/150‑1 (MQ=255)
                                                                                        ccTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGATATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTg                                                       <  2:2644969/150‑1 (MQ=255)
                                                                                         cTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGa                                                      <  2:1335055/150‑1 (MQ=255)
                                                                                          tGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGAt                                                     >  1:2911257/1‑150 (MQ=255)
                                                                                          tGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGAt                                                     >  1:2096948/1‑150 (MQ=255)
                                                                                           gCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATc                                                    <  1:2373276/150‑1 (MQ=255)
                                                                                              gCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGtt                                                 >  1:1059533/1‑150 (MQ=255)
                                                                                                tACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTCTGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCa                                               >  2:849899/1‑150 (MQ=255)
                                                                                                tACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCa                                               >  2:1637060/1‑150 (MQ=255)
                                                                                                  ccTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAgg                                             <  2:2855130/150‑1 (MQ=255)
                                                                                                  ccTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAgg                                             >  2:4684749/1‑150 (MQ=255)
                                                                                                   ctgctgGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGa                                            >  1:794275/1‑150 (MQ=255)
                                                                                                     gctgGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATa                                          >  2:1931830/1‑150 (MQ=255)
                                                                                                       tgGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAg                                        >  2:2108725/1‑150 (MQ=255)
                                                                                                       tgGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAg                                        >  2:3191034/1‑150 (MQ=255)
                                                                                                       tgGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAg                                        >  1:4619173/1‑150 (MQ=255)
                                                                                                          ttCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGaaa                                     >  1:3523827/1‑150 (MQ=255)
                                                                                                                ccGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAgc                               <  1:1476161/150‑1 (MQ=255)
                                                                                                                 cGCATCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAgcg                              <  1:2543368/150‑1 (MQ=255)
                                                                                                                 cGCATCAGCACGGCAAAACCGGGCGACAGGTGGCGATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAgcg                              >  1:990201/1‑150 (MQ=255)
                                                                                                                    aTCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCg                           >  2:712862/1‑150 (MQ=255)
                                                                                                                    aTCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCg                           >  2:921087/1‑150 (MQ=255)
                                                                                                                    aTCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCg                           <  1:1265152/150‑1 (MQ=255)
                                                                                                                    aTCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCg                           <  2:1048573/150‑1 (MQ=255)
                                                                                                                    aTCAGCACGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCg                           >  1:3788174/1‑150 (MQ=255)
                                                                                                                     tcatctcGGCATTACCGGGCGACAGGTGGCTATGTGGATGGCTGGGCTGTTGTTTATTGAAGATGACGAGATCCTGAAATTAATGCTGCAATAACAGTGTGGTATCGATGATGTTCCGGTTATCGTTGAGGATAAGAAATTTAGCGCCGa                          <  1:1133409/144‑1 (MQ=255)
                                                                                                                          aCGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCAGACGGGc                     >  2:1785735/1‑150 (MQ=255)
                                                                                                                           cGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCa                    >  1:4016077/1‑150 (MQ=255)
                                                                                                                           cGGCAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCa                    <  1:4263496/150‑1 (MQ=255)
                                                                                                                              cAAAACCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAgat                 >  1:3698055/1‑150 (MQ=255)
                                                                                                                               aaacCCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAgatt                <  2:3035356/150‑1 (MQ=255)
                                                                                                                               aaaaCCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAgatt                >  1:3875166/1‑150 (MQ=255)
                                                                                                                                aaaCCGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAgattg               >  2:675690/1‑150 (MQ=255)
                                                                                                                                   ccGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAgattgatt            >  2:1458854/1‑150 (MQ=255)
                                                                                                                                    cGGGCGACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTa           >  1:3113722/1‑150 (MQ=255)
                                                                                                                                         gACAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAAc      <  1:1498873/150‑1 (MQ=255)
                                                                                                                                          aCAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACt     <  1:4725133/150‑1 (MQ=255)
                                                                                                                                          aCAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACt     >  1:4874958/1‑150 (MQ=255)
                                                                                                                                           cAGGTGGCTATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTg    <  2:2832283/150‑1 (MQ=255)
                                                                                                                                           cAGGTGGCGATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGAGTGATTATCAACTg    >  2:4957645/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCTGGAAGTACCGGGTGAAGTCGACGGCGGCCCGCAGGGAATTATTCCGCCAGGTGGCAAGCGCTCGGTGACGTTGAACGTTGATCAACCTGCCGCTACCTGCTGGTTCCATCCGCATCAGCACGGCAAAACCGGGCGACAGGTGGCGATGGGGCTGGCTGGGCTGGTGGTGATTGAAGATGACGAGATCCTGAAATTAATGCTGCCAAAACAGTGGGGTATCGATGATGTTCCGGTGATCGTTCAGGATAAGAAATTTAGCGCCGACGGGCAGATTGATTATCAACTGGA  >  CP009273/133881‑134171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑