Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,580,852 C→A 100% intergenic (‑109/+225) ydeP ← / ← ydeQ putative oxidoreductase/putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,580,8520CA98.0% 655.7 / 3.3 204intergenic (‑109/+225)ydeP/ydeQputative oxidoreductase/putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (105/95);  total (107/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

ACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGGTGTAG  >  CP009273/1580705‑1581001
                                                                                                                                                   |                                                                                                                                                     
accTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTActatc                                                                                                                                                     >  2:3230706/1‑150 (MQ=255)
 ccTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTActatct                                                                                                                                                    >  2:795137/1‑150 (MQ=255)
   tGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCttt                                                                                                                                                  <  2:1233249/150‑1 (MQ=255)
       gcaCCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTAt                                                                                                                                              <  1:1109578/150‑1 (MQ=255)
           ccTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTAttttt                                                                                                                                          <  1:1741501/150‑1 (MQ=255)
              ggTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAAt                                                                                                                                       >  2:4533979/1‑150 (MQ=255)
               gTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATc                                                                                                                                      >  1:865833/1‑150 (MQ=255)
                tAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCt                                                                                                                                     >  2:588118/1‑150 (MQ=255)
                  ggATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCtat                                                                                                                                   >  2:227132/1‑150 (MQ=255)
                  ggATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCtat                                                                                                                                   <  2:1682408/150‑1 (MQ=255)
                    aTTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCtatta                                                                                                                                 <  1:4887689/150‑1 (MQ=255)
                         aTTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGtt                                                                                                                            <  2:4262607/150‑1 (MQ=255)
                         aTTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGtt                                                                                                                            >  2:189740/1‑150 (MQ=255)
                         aTTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGtt                                                                                                                            <  1:1158826/150‑1 (MQ=255)
                          tttttttCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTa                                                                                                                           <  1:4864865/150‑1 (MQ=255)
                              tttCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACa                                                                                                                       >  2:50060/1‑150 (MQ=255)
                              tttCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACa                                                                                                                       >  1:4440857/1‑150 (MQ=255)
                              tttCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACa                                                                                                                       <  2:4466896/150‑1 (MQ=255)
                                tcttcATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAAt                                                                                                                     <  1:1566179/150‑1 (MQ=255)
                                 cttcATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATa                                                                                                                    >  1:2491824/1‑150 (MQ=255)
                                   tcATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGa                                                                                                                  >  2:2402227/1‑150 (MQ=255)
                                     aTCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGAtt                                                                                                                >  2:359970/1‑150 (MQ=255)
                                     aTCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGAtt                                                                                                                >  2:2179890/1‑150 (MQ=255)
                                            tATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACg                                                                                                         >  1:2178827/1‑150 (MQ=255)
                                            tATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACg                                                                                                         >  1:4763173/1‑150 (MQ=255)
                                               ccTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATa                                                                                                      <  1:3199974/150‑1 (MQ=255)
                                                 tgtgtgGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAg                                                                                                    >  2:465538/1‑150 (MQ=255)
                                                 tgtgtgGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAg                                                                                                    <  2:673547/150‑1 (MQ=255)
                                                 tgtgtgGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAg                                                                                                    >  2:2926596/1‑150 (MQ=255)
                                                   tgtgGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGtt                                                                                                  <  2:4498606/150‑1 (MQ=255)
                                                    gtgGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTa                                                                                                 >  2:3258461/1‑150 (MQ=255)
                                                    gtgGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTa                                                                                                 >  2:3258470/1‑150 (MQ=255)
                                                      ggAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAg                                                                                               >  2:3093757/1‑150 (MQ=255)
                                                       gAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGc                                                                                              >  2:3510295/1‑150 (MQ=255)
                                                          aTGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatc                                                                                           >  1:3258010/1‑150 (MQ=255)
                                                           tGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATTCAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatca                                                                                          <  1:2298871/150‑1 (MQ=255)
                                                           tGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatca                                                                                          <  2:728920/150‑1 (MQ=255)
                                                            gAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatcat                                                                                         <  1:3979528/150‑1 (MQ=255)
                                                            gAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGcatcat                                                                                         >  1:3567090/1‑150 (MQ=255)
                                                              attattAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtct                                                                                       >  2:477855/1‑150 (MQ=255)
                                                              attattAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtct                                                                                       <  1:3732514/150‑1 (MQ=255)
                                                               ttattaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATTGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATATATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtctc                                                                                      <  1:3008322/150‑1 (MQ=255)
                                                                tattaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtctct                                                                                     >  1:2389283/1‑150 (MQ=255)
                                                                tattaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtctct                                                                                     <  2:1589753/150‑1 (MQ=255)
                                                                tattaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtctct                                                                                     >  1:3401282/1‑150 (MQ=255)
                                                                 attaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtctctc                                                                                    >  1:2901833/1‑150 (MQ=255)
                                                                 attaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCAtctctc                                                                                    >  2:3450742/1‑150 (MQ=255)
                                                                  ttaGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCa                                                                                   >  2:1186851/1‑150 (MQ=255)
                                                                   taGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCAt                                                                                  <  1:4405321/150‑1 (MQ=255)
                                                                   taGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCAt                                                                                  >  1:1367414/1‑150 (MQ=255)
                                                                   taGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCAt                                                                                  <  1:3916528/150‑1 (MQ=255)
                                                                    aGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATa                                                                                 >  1:4242366/1‑150 (MQ=255)
                                                                    aGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATa                                                                                 <  1:3800809/150‑1 (MQ=255)
                                                                       aTTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgtg                                                                              >  1:1216608/1‑150 (MQ=255)
                                                                        tttGTAATATCGATGATGCACAGTACATACAAATGGATTATCGCCAACATTGATATATAAGAGTAGGGGACTACTAAATTTCTATTTTTAATATATTATAGTTAAAAAATAGATTAAATACGATAAGTTAAGCATCATAACTCATAgtgt                                                                             >  2:2034233/1‑150 (MQ=255)
                                                                        tttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgtgt                                                                             <  1:584459/150‑1 (MQ=255)
                                                                        tttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgtgt                                                                             >  1:2702718/1‑150 (MQ=255)
                                                                        tttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAgtgt                                                                             >  1:106330/1‑150 (MQ=255)
                                                                        tttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGAATCATCTCTCATAgtgt                                                                             >  1:106310/1‑150 (MQ=255)
                                                                         ttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGtt                                                                            <  2:5123285/150‑1 (MQ=255)
                                                                         ttGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGtt                                                                            >  1:4710941/1‑150 (MQ=255)
                                                                          tGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGttt                                                                           <  1:984839/150‑1 (MQ=255)
                                                                          tGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGAGttt                                                                           >  1:3039452/1‑150 (MQ=255)
                                                                           gTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTa                                                                          <  2:3429596/150‑1 (MQ=255)
                                                                           gTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTa                                                                          <  1:1044343/150‑1 (MQ=255)
                                                                             aaTAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTaaa                                                                        >  1:1085602/1‑150 (MQ=255)
                                                                                aGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTa                                                                     >  2:463778/1‑150 (MQ=255)
                                                                                      gatgCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACatat                                                               >  1:5056543/1‑150 (MQ=255)
                                                                                          cacaGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATAttttt                                                           <  2:2324237/150‑1 (MQ=255)
                                                                                             aGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCaa                                                        >  2:2128433/1‑150 (MQ=255)
                                                                                                   tacaAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATaaa                                                  >  1:1281494/1‑150 (MQ=255)
                                                                                                      aaaGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATaaaaaa                                               >  2:538211/1‑150 (MQ=255)
                                                                                                      aaaGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATaaaaaa                                               >  1:2215760/1‑150 (MQ=255)
                                                                                                         gggATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATaa                                            <  2:872747/150‑1 (MQ=255)
                                                                                                            aTTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGAc                                         <  2:4434163/150‑1 (MQ=255)
                                                                                                            aTTATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGAc                                         <  2:4138719/150‑1 (MQ=255)
                                                                                                              tATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGActc                                       >  2:1987093/1‑150 (MQ=255)
                                                                                                              tATCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGActc                                       >  1:4909825/1‑150 (MQ=255)
                                                                                                                tCGCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTg                                     >  2:570430/1‑150 (MQ=255)
                                                                                                                  gCCAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGc                                   <  2:3754201/150‑1 (MQ=255)
                                                                                                                    cAACATGGATATATACGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCtt                                 <  1:4021339/150‑1 (MQ=255)
                                                                                                                    cAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCtt                                 <  1:963521/150‑1 (MQ=255)
                                                                                                                    cAACATGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCtt                                 <  2:3403394/150‑1 (MQ=255)
                                                                                                                        aTGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAAt                             >  2:338880/1‑150 (MQ=255)
                                                                                                                         tGGATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAAtt                            <  2:4891445/150‑1 (MQ=255)
                                                                                                                          ggATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATtg                           <  2:2826941/150‑1 (MQ=255)
                                                                                                                           gATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATATATTAAATAAGATATGTTAAGCATCATATCTCATAGTGTTTAAATTATCATATTTTTCAATATCATAAATATGAATGTTGCTTAAAgtgt                          >  1:2621780/1‑150 (MQ=255)
                                                                                                                           gATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATtgt                          >  2:932378/1‑150 (MQ=255)
                                                                                                                           gATATATAAGAGTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATtgt                          <  1:1416073/150‑1 (MQ=255)
                                                                                                                                ataAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGcgcg                     >  1:182107/1‑150 (MQ=255)
                                                                                                                                   agagTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGAt                  <  1:2608493/150‑1 (MQ=255)
                                                                                                                                     agTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGAttt                >  1:2124707/1‑150 (MQ=255)
                                                                                                                                      gTAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGAtttt               <  2:3332598/150‑1 (MQ=255)
                                                                                                                                       tAGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTc              >  1:2738609/1‑150 (MQ=255)
                                                                                                                                        aGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCt             <  2:4710941/150‑1 (MQ=255)
                                                                                                                                        aGGGGACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCt             >  2:385958/1‑150 (MQ=255)
                                                                                                                                           ggACTACTATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTAc          <  1:3754043/150‑1 (MQ=255)
                                                                                                                                               tactATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGgt      >  1:1658057/1‑150 (MQ=255)
                                                                                                                                               tactATCTTTCTATTTTTAATATATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGgt      <  2:3906280/150‑1 (MQ=255)
                                                                                                                                                actATCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGgtg     >  2:2757732/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGGTGTAG  >  CP009273/1580705‑1581001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑