Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,071 G→T 100% I214I (ATC→ATA)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,0710GT98.5% 1066.2 / 4.8 330I214I (ATC→ATA) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (149/176);  total (151/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 330 total aligned reads displayed.

TACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGAT  >  CP009273/136924‑137219
                                                                                                                                                   |                                                                                                                                                    
 aCGTGCTGGAAAGACTCGTTGTTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAtt                                                                                                                                                   <  1:4429853/150‑1 (MQ=255)
  cGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAttt                                                                                                                                                  >  1:3954318/1‑150 (MQ=255)
   gTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTc                                                                                                                                                 <  1:3371110/150‑1 (MQ=255)
    tGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCa                                                                                                                                                >  2:1926700/1‑150 (MQ=255)
          taaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGa                                                                                                                                          >  2:1190116/2‑150 (MQ=255)
          aaaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGa                                                                                                                                          >  2:4086326/1‑150 (MQ=255)
          aaaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGa                                                                                                                                          <  2:1336754/150‑1 (MQ=255)
          aaaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGa                                                                                                                                          <  2:1609904/150‑1 (MQ=255)
            aGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATc                                                                                                                                        <  1:1209012/150‑1 (MQ=255)
            aGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATc                                                                                                                                        >  2:4744021/1‑150 (MQ=255)
             gACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCg                                                                                                                                       <  1:1502500/150‑1 (MQ=255)
             gACTCGTTGGTTGTCAGCTCAGGATCGTAATGCCATGTCTCTGTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCg                                                                                                                                       <  2:219065/150‑1 (MQ=255)
              aCTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGt                                                                                                                                      >  2:4785600/1‑150 (MQ=255)
               cTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGtt                                                                                                                                     <  1:1445351/150‑1 (MQ=255)
                tCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTc                                                                                                                                    <  1:893467/150‑1 (MQ=255)
                tCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTc                                                                                                                                    <  1:3680679/150‑1 (MQ=255)
                tCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTc                                                                                                                                    <  1:4794527/150‑1 (MQ=255)
                tCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTc                                                                                                                                    <  2:3555190/150‑1 (MQ=255)
                  gTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCgg                                                                                                                                  <  1:4352713/150‑1 (MQ=255)
                  gTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCgg                                                                                                                                  >  2:3141189/1‑150 (MQ=255)
                   ttGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgc                                                                                                                                 >  1:4173227/1‑150 (MQ=255)
                   ttGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTNTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgc                                                                                                                                 >  1:2681055/1‑150 (MQ=255)
                    tGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgct                                                                                                                                <  2:3290603/150‑1 (MQ=255)
                    tGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgct                                                                                                                                <  2:4185345/150‑1 (MQ=255)
                       ttttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctgct                                                                                                                             >  2:583839/1‑150 (MQ=255)
                        tttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCtt                                                                                                                            >  2:4160721/1‑150 (MQ=255)
                         ttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTc                                                                                                                           <  2:3742943/150‑1 (MQ=255)
                            aGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                        >  1:2368124/1‑150 (MQ=255)
                               tCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCg                                                                                                                     >  2:4244161/1‑150 (MQ=255)
                                cAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGc                                                                                                                    >  1:2301817/1‑150 (MQ=255)
                                 aGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGcc                                                                                                                   >  1:4569453/1‑150 (MQ=255)
                                 aGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGcc                                                                                                                   >  2:1017235/1‑150 (MQ=255)
                                  ggATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCa                                                                                                                  >  2:2274517/1‑150 (MQ=255)
                                  ggATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCa                                                                                                                  >  2:2462662/1‑150 (MQ=255)
                                  ggATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCa                                                                                                                  >  1:2619470/1‑150 (MQ=255)
                                   gATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAg                                                                                                                 >  1:4449597/1‑150 (MQ=255)
                                    aTCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGt                                                                                                                <  1:2270436/150‑1 (MQ=255)
                                    aTCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGt                                                                                                                <  1:3893934/150‑1 (MQ=255)
                                       gTAATGCCATTTCTCTTTGGGGGGGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACg                                                                                                             >  2:1746789/1‑150 (MQ=255)
                                          aTGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGaa                                                                                                          >  1:1937642/1‑150 (MQ=255)
                                          aTGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGaa                                                                                                          <  2:1476782/150‑1 (MQ=255)
                                           tGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAac                                                                                                         >  1:832199/1‑150 (MQ=255)
                                            gCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAaca                                                                                                        <  2:237895/150‑1 (MQ=255)
                                               aTTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAccc                                                                                                     >  1:1306648/1‑150 (MQ=255)
                                                tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCa                                                                                                    >  2:1068987/1‑150 (MQ=255)
                                                tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCa                                                                                                    <  1:1136739/150‑1 (MQ=255)
                                                  tctcTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAgg                                                                                                  >  2:2594305/1‑150 (MQ=255)
                                                    tctTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGct                                                                                                >  1:198801/1‑150 (MQ=255)
                                                    tctTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGct                                                                                                >  1:2793277/1‑150 (MQ=255)
                                                       ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttct                                                                                             >  2:343191/1‑150 (MQ=255)
                                                       ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttct                                                                                             <  2:4936116/150‑1 (MQ=255)
                                                         gCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCttt                                                                                           <  2:2877705/150‑1 (MQ=255)
                                                         gCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCttt                                                                                           >  2:4447543/1‑150 (MQ=255)
                                                          ccGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTc                                                                                          <  1:1676410/150‑1 (MQ=255)
                                                           cGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCa                                                                                         >  1:4735538/1‑150 (MQ=255)
                                                              tGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAt                                                                                      >  1:2499938/1‑150 (MQ=255)
                                                                gcggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTa                                                                                    <  1:1260612/150‑1 (MQ=255)
                                                                 cggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTAt                                                                                   <  2:4655993/150‑1 (MQ=255)
                                                                  ggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATg                                                                                  <  1:4124941/150‑1 (MQ=255)
                                                                   gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATgg                                                                                 <  2:2137311/150‑1 (MQ=255)
                                                                    cATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGa                                                                                <  1:5162608/150‑1 (MQ=255)
                                                                     aTCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGAc                                                                               >  2:1693375/1‑150 (MQ=255)
                                                                      tCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACg                                                                              >  2:3053605/1‑150 (MQ=255)
                                                                       cAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGt                                                                             <  1:2120192/150‑1 (MQ=255)
                                                                       cAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGt                                                                             <  1:1778837/150‑1 (MQ=255)
                                                                         aGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTa                                                                           <  1:1258048/150‑1 (MQ=255)
                                                                         aGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTa                                                                           >  2:1526584/1‑150 (MQ=255)
                                                                            gcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCg                                                                        <  1:3615213/150‑1 (MQ=255)
                                                                             cAAACAGGCGCTGGTGAGCGGGACACAGGTAAAGGGGGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCgg                                                                       <  2:1831474/150‑1 (MQ=255)
                                                                                 cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCAtt                                                                   <  2:2318250/150‑1 (MQ=255)
                                                                                  aGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTa                                                                  <  1:3447312/150‑1 (MQ=255)
                                                                                     cgcTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAAtt                                                               >  2:3304511/1‑150 (MQ=255)
                                                                                       cTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTg                                                             >  2:3909112/1‑150 (MQ=255)
                                                                                           tGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTc                                                         <  1:4982907/150‑1 (MQ=255)
                                                                                              gCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgc                                                      <  1:2069997/150‑1 (MQ=255)
                                                                                               cGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcg                                                     <  1:182478/150‑1 (MQ=255)
                                                                                                         gTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCg                                           <  2:4046631/150‑1 (MQ=255)
                                                                                                            aaGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTg                                        >  1:4654468/1‑150 (MQ=255)
                                                                                                              gggTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAc                                      <  1:1706322/150‑1 (MQ=255)
                                                                                                               ggTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAcc                                     <  1:3190542/150‑1 (MQ=255)
                                                                                                                      cccACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGa                              <  2:4597573/150‑1 (MQ=255)
                                                                                                                         aCTTTAATCGGCGTCACCTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTa                           >  2:3235055/1‑150 (MQ=255)
                                                                                                                          cTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTAc                          >  1:3077540/1‑150 (MQ=255)
                                                                                                                           tttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACg                         <  2:847370/150‑1 (MQ=255)
                                                                                                                            ttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGa                        >  1:278131/1‑150 (MQ=255)
                                                                                                                            ttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGa                        >  1:2429066/1‑150 (MQ=255)
                                                                                                                             tAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAc                       <  2:1987421/150‑1 (MQ=255)
                                                                                                                             tAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAc                       <  2:4449613/150‑1 (MQ=255)
                                                                                                                                tCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCAt                    <  1:5092222/150‑1 (MQ=255)
                                                                                                                                tCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCAt                    >  1:2950692/1‑150 (MQ=255)
                                                                                                                                 cGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATa                   >  1:4253629/1‑150 (MQ=255)
                                                                                                                                  ggCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATag                  >  2:1106728/1‑150 (MQ=255)
                                                                                                                                  ggCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTACACATCGCCAGTACGGAACACCCAGGCATCTTTCAGATTATGGAAGTTATCGGCGTTAATTTTTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATag                  >  1:797122/1‑150 (MQ=255)
                                                                                                                                     gTCACTTGATTGGTTATTTCACCCGGCTCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTATTTCAGATTATGGACGTTATCGGCGTTATTTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggca               <  1:675525/150‑1 (MQ=255)
                                                                                                                                     gTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggca               <  2:2903067/150‑1 (MQ=255)
                                                                                                                                     gTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggca               <  2:4205622/150‑1 (MQ=255)
                                                                                                                                       cACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggcagg             >  1:3993495/1‑150 (MQ=255)
                                                                                                                                         cTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGcc           >  1:4048595/1‑150 (MQ=255)
                                                                                                                                                ggTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTg    <  1:3386045/150‑1 (MQ=255)
                                                                                                                                                  tGATTTCACCCGGATCGTTCGGCTGCGTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGAt  <  1:3821337/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGAT  >  CP009273/136924‑137219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑