Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,669,592 G→T 100% F342L (TTC→TTA)  pntB ← pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,669,5920GT96.8% 982.2 / 18.5 315F342L (TTC→TTA) pntBpyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (8/2);  new base T (141/164);  total (149/166)
Fisher's exact test for biased strand distribution p-value = 5.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 315 total aligned reads displayed.

GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGAT  >  CP009273/1669445‑1669738
                                                                                                                                                   |                                                                                                                                                  
gTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaa                                                                                                                                                  >  2:2395446/1‑150 (MQ=255)
 ttAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaaa                                                                                                                                                 >  2:2112311/1‑150 (MQ=255)
 ttAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaaa                                                                                                                                                 >  1:2089752/1‑150 (MQ=255)
  tAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAAc                                                                                                                                                <  2:149138/150‑1 (MQ=255)
   aGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACg                                                                                                                                               >  1:3664852/1‑150 (MQ=255)
       ccAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGcaca                                                                                                                                           >  2:1931668/1‑150 (MQ=255)
        cAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAt                                                                                                                                          >  2:816454/1‑150 (MQ=255)
         aaTCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAtt                                                                                                                                         >  2:2832697/1‑150 (MQ=255)
               cattACGGTATCGGTATCAGCAAAGTCATATTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATAcc                                                                                                                                   <  1:2247933/147‑1 (MQ=255)
               cAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATAcc                                                                                                                                   <  2:196896/150‑1 (MQ=255)
                aGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCa                                                                                                                                  >  2:796131/1‑150 (MQ=255)
                aGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCa                                                                                                                                  <  2:1119665/150‑1 (MQ=255)
                  tACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACg                                                                                                                                <  1:2881552/150‑1 (MQ=255)
                    cggtatcggtatCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAg                                                                                                                              <  2:1460783/150‑1 (MQ=255)
                       tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcgc                                                                                                                           >  1:2432581/1‑150 (MQ=255)
                        atcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcgcg                                                                                                                          <  2:3468646/150‑1 (MQ=255)
                         tcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcgcgc                                                                                                                         >  2:2133196/1‑150 (MQ=255)
                            gtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACTATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAt                                                                                                                      >  1:1718735/1‑150 (MQ=255)
                              atcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAttt                                                                                                                    <  2:4698867/150‑1 (MQ=255)
                                    aaaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGt                                                                                                              >  2:4451541/1‑150 (MQ=255)
                                    aaaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGt                                                                                                              >  1:931961/1‑150 (MQ=255)
                                    aaaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGt                                                                                                              >  1:4352553/1‑150 (MQ=255)
                                     aaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                             <  2:5070862/150‑1 (MQ=255)
                                     aaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                             <  2:1329704/150‑1 (MQ=255)
                                     aaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                             <  2:4559662/150‑1 (MQ=255)
                                      aGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                            <  1:4637483/150‑1 (MQ=255)
                                       gTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAt                                                                                                           <  1:873913/150‑1 (MQ=255)
                                         catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAttt                                                                                                         <  2:2954770/150‑1 (MQ=255)
                                             atTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGc                                                                                                     >  1:4047279/1‑150 (MQ=255)
                                             atTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGc                                                                                                     >  2:2429613/1‑150 (MQ=255)
                                              ttGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCg                                                                                                    <  2:4384473/150‑1 (MQ=255)
                                               tGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                                   <  2:167129/150‑1 (MQ=255)
                                                gATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                  >  2:1431209/1‑150 (MQ=255)
                                                 aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                                 <  1:3634254/150‑1 (MQ=255)
                                                 aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                                 <  1:1608343/150‑1 (MQ=255)
                                                   ctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                               <  2:744631/150‑1 (MQ=255)
                                                   ctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGAGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                               >  2:694485/1‑150 (MQ=255)
                                                      gTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGata                                                                                            >  1:4999959/1‑150 (MQ=255)
                                                        ccATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAtt                                                                                          >  1:3193822/1‑150 (MQ=255)
                                                        ccATGTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAtt                                                                                          <  2:1385114/150‑1 (MQ=255)
                                                            ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgcgc                                                                                      >  2:4484557/1‑150 (MQ=255)
                                                             tCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGCATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGcc                                                                                     <  1:307440/150‑1 (MQ=255)
                                                               cAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTg                                                                                   <  2:4983680/150‑1 (MQ=255)
                                                               cAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTg                                                                                   <  2:4031856/150‑1 (MQ=255)
                                                                aGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgc                                                                                  <  1:353896/150‑1 (MQ=255)
                                                                  cACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                                <  1:3337989/150‑1 (MQ=255)
                                                                      aTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                            <  1:2362201/150‑1 (MQ=255)
                                                                        gTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGc                                                                          <  2:3319572/150‑1 (MQ=255)
                                                                           aTACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCAt                                                                       >  2:2033944/1‑150 (MQ=255)
                                                                            tACGGTACTTTTGCTACAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTTATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                      <  1:107956/150‑1 (MQ=255)
                                                                              cGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGcc                                                                    <  1:4899670/150‑1 (MQ=255)
                                                                               ggTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCg                                                                   <  2:179563/150‑1 (MQ=255)
                                                                                gTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                  >  2:2344332/1‑150 (MQ=255)
                                                                                 tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTa                                                                 >  2:3280264/1‑150 (MQ=255)
                                                                                 tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTa                                                                 <  1:3153478/150‑1 (MQ=255)
                                                                                  aCTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAc                                                                >  2:4721709/1‑150 (MQ=255)
                                                                                     tttGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAcccc                                                             <  2:5145541/150‑1 (MQ=255)
                                                                                     tttGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAcccc                                                             <  2:2471649/150‑1 (MQ=255)
                                                                                       tGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCgg                                                           <  1:525903/150‑1 (MQ=255)
                                                                                        gCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                          <  1:3733343/150‑1 (MQ=255)
                                                                                         cTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                         <  2:2842930/150‑1 (MQ=255)
                                                                                           tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTa                                                       >  1:1165704/1‑150 (MQ=255)
                                                                                            cagccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                      >  2:651825/1‑150 (MQ=255)
                                                                                             agccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                     <  2:4853569/150‑1 (MQ=255)
                                                                                             agccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                     >  1:746113/1‑150 (MQ=255)
                                                                                              gccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATg                                                    >  1:7203/1‑150 (MQ=255)
                                                                                               ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGa                                                   >  1:2013009/1‑150 (MQ=255)
                                                                                                   cAATACGTTCATATGTCCAGGCAAACGCCACGCGACAGGTTGGATACCTAAACGCACATTAATACCAAGAGCGCGAAATTTCTCAGTAATTTCATCGACAGGATATTGCGACTGAGCGACTTCCATGCCGTACAACGGAGAAATGATCAc                                               >  1:4478559/1‑150 (MQ=255)
                                                                                                      tACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGa                                            <  2:1702915/150‑1 (MQ=255)
                                                                                                       aCGTTCATATGTACAGGCAAACGCCCCGCGACCGGGTGGATACCTAATCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGaa                                           <  2:4457735/150‑1 (MQ=255)
                                                                                                         gTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                         >  1:1253275/1‑150 (MQ=255)
                                                                                                         gTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                         <  2:4335283/150‑1 (MQ=255)
                                                                                                           tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                       <  1:621077/150‑1 (MQ=255)
                                                                                                           tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                       <  2:2918813/150‑1 (MQ=255)
                                                                                                             atatGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAg                                     <  1:828370/150‑1 (MQ=255)
                                                                                                                   ccAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTc                               >  2:3226137/1‑150 (MQ=255)
                                                                                                                    cAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTca                              <  1:3992892/150‑1 (MQ=255)
                                                                                                                    cAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTca                              >  1:1842967/1‑150 (MQ=255)
                                                                                                                    cAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTca                              >  1:3939107/1‑150 (MQ=255)
                                                                                                                     aGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGACTGGGAGTTTTTcag                             >  2:1259858/1‑150 (MQ=255)
                                                                                                                      ggCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagc                            >  1:4748372/1‑150 (MQ=255)
                                                                                                                        cAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                          >  2:4189191/1‑150 (MQ=255)
                                                                                                                        cAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                          <  2:3664852/150‑1 (MQ=255)
                                                                                                                         aaaCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                         >  2:4111480/1‑150 (MQ=255)
                                                                                                                         aaaCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                         >  1:342002/1‑150 (MQ=255)
                                                                                                                         aaaCGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                         >  1:4252760/1‑150 (MQ=255)
                                                                                                                          aaCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGtt                        >  1:1911406/1‑150 (MQ=255)
                                                                                                                            cGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTcc                      <  1:60722/150‑1 (MQ=255)
                                                                                                                                  gcgACCGTGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtc                <  1:509599/150‑1 (MQ=255)
                                                                                                                                   cgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtct               <  2:479659/150‑1 (MQ=255)
                                                                                                                                   cgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtct               >  2:383387/1‑150 (MQ=255)
                                                                                                                                   cgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCGGtct               <  2:578164/150‑1 (MQ=255)
                                                                                                                                    gACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtctc              <  1:141359/150‑1 (MQ=255)
                                                                                                                                        gggTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtcttct          >  2:3113885/1‑150 (MQ=255)
                                                                                                                                        gggTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtcttct          <  1:2637233/150‑1 (MQ=255)
                                                                                                                                         ggTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTg         <  1:506214/150‑1 (MQ=255)
                                                                                                                                          gTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGc        <  1:4451541/150‑1 (MQ=255)
                                                                                                                                          gTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGc        <  2:1889132/150‑1 (MQ=255)
                                                                                                                                               tACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGa   >  1:2223850/1‑150 (MQ=255)
                                                                                                                                                aCCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGAt  <  1:2723987/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGAT  >  CP009273/1669445‑1669738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑