Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,680,642 C→A 100% E68D (GAG→GAT)  fumC ← fumarate hydratase (fumarase C),aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,680,6420CA97.2% 902.1 / 10.7 287E68D (GAG→GAT) fumCfumarate hydratase (fumarase C),aerobic Class II
Reads supporting (aligned to +/- strand):  ref base C (3/5);  new base A (121/158);  total (124/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 289 total aligned reads displayed.

TAATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTT  >  CP009273/1680495‑1680788
                                                                                                                                                   |                                                                                                                                                  
 aaTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTatct                                                                                                                                                 >  2:3966522/1‑150 (MQ=255)
   ttCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTc                                                                                                                                               >  1:1305035/1‑150 (MQ=255)
     cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAg                                                                                                                                             <  2:5158178/150‑1 (MQ=255)
     cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAg                                                                                                                                             <  1:3708309/150‑1 (MQ=255)
     cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAg                                                                                                                                             <  1:2791285/150‑1 (MQ=255)
      aCTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGa                                                                                                                                            >  2:153851/1‑150 (MQ=255)
        tGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGaca                                                                                                                                          <  1:1037736/150‑1 (MQ=255)
        tGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGaca                                                                                                                                          <  1:1783926/150‑1 (MQ=255)
        tGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTGTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGaca                                                                                                                                          <  1:2127113/150‑1 (MQ=255)
         ggCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGacaa                                                                                                                                         <  1:4951130/150‑1 (MQ=255)
         ggCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGacaa                                                                                                                                         <  1:1867396/150‑1 (MQ=255)
           cccGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGacaaca                                                                                                                                       >  2:76043/1‑150 (MQ=255)
            ccGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGAcaacaa                                                                                                                                      >  2:4177404/1‑150 (MQ=255)
             cGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAg                                                                                                                                     <  1:3143616/150‑1 (MQ=255)
              ggTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCACTCGCTTTATCTTCAGACAACAAGc                                                                                                                                    >  2:3446742/1‑150 (MQ=255)
                 tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa                                                                                                                                 <  2:3103274/150‑1 (MQ=255)
                 tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa                                                                                                                                 <  2:5019180/150‑1 (MQ=255)
                 tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa                                                                                                                                 <  1:2698700/150‑1 (MQ=255)
                 tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa                                                                                                                                 <  2:4090890/150‑1 (MQ=255)
                  aGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTaa                                                                                                                                >  2:1059748/1‑150 (MQ=255)
                  aGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTaa                                                                                                                                <  1:3260529/150‑1 (MQ=255)
                   gCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTaaa                                                                                                                               >  2:1414157/1‑150 (MQ=255)
                    ccAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGTCGCTCGCTTTATCTTCAGACAACAAGCCTAAAt                                                                                                                              <  1:3331500/150‑1 (MQ=255)
                     cAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATc                                                                                                                             <  1:3503188/150‑1 (MQ=255)
                       gCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCtt                                                                                                                           <  1:2941880/150‑1 (MQ=255)
                         aCTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCa                                                                                                                         <  2:3463788/150‑1 (MQ=255)
                         aCTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCa                                                                                                                         <  1:1375307/150‑1 (MQ=255)
                          cTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCAt                                                                                                                        >  1:2756477/1‑150 (MQ=255)
                           ttCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCAtt                                                                                                                       <  2:1997871/150‑1 (MQ=255)
                            tCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTa                                                                                                                      >  1:5089450/1‑150 (MQ=255)
                             cGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTaa                                                                                                                     <  1:2303016/150‑1 (MQ=255)
                             cGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGCCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTaa                                                                                                                     >  1:195250/1‑150 (MQ=255)
                               ttcatgttcatgTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACt                                                                                                                   >  2:3840866/1‑150 (MQ=255)
                                   tgttcttgttTCTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTAGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTg                                                                                                               <  2:4823369/150‑1 (MQ=255)
                                     ttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGcc                                                                                                             <  2:3479618/150‑1 (MQ=255)
                                     ttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGcc                                                                                                             >  1:79969/1‑150 (MQ=255)
                                      tcatgtgACTTTGCGTGCCGGAGCCTGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCg                                                                                                            <  1:1907646/150‑1 (MQ=255)
                                        atgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCt                                                                                                          <  1:2487917/150‑1 (MQ=255)
                                        atgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCt                                                                                                          <  2:2480624/150‑1 (MQ=255)
                                                   cGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGt                                                                                               >  1:4305064/1‑150 (MQ=255)
                                                   cGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGt                                                                                               >  2:2168343/1‑150 (MQ=255)
                                                    gTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGtt                                                                                              <  1:4375757/150‑1 (MQ=255)
                                                    gTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGtt                                                                                              <  1:2585559/150‑1 (MQ=255)
                                                     tGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTa                                                                                             >  1:4977807/1‑150 (MQ=255)
                                                     tGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAAGTCGTCGTCATGCTGTCCTGCCAGTACGTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTa                                                                                             <  1:1916328/150‑1 (MQ=255)
                                                         ggAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc                                                                                         >  2:4677479/1‑150 (MQ=255)
                                                             ccGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgc                                                                                     <  2:1929019/150‑1 (MQ=255)
                                                              cGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAgcg                                                                                    >  2:605479/1‑150 (MQ=255)
                                                                gTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCa                                                                                  >  2:855715/1‑150 (MQ=255)
                                                                   tGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGa                                                                               <  2:2919257/150‑1 (MQ=255)
                                                                     ccAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAAt                                                                             >  2:2450968/1‑150 (MQ=255)
                                                                     ccAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAAt                                                                             >  2:2280207/1‑150 (MQ=255)
                                                                      cAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATc                                                                            <  2:1818919/150‑1 (MQ=255)
                                                                      cAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATc                                                                            <  2:907533/150‑1 (MQ=255)
                                                                        gATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAg                                                                          <  2:2555140/150‑1 (MQ=255)
                                                                         aTAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGt                                                                         <  2:3660453/150‑1 (MQ=255)
                                                                            gCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAg                                                                      <  2:3119181/150‑1 (MQ=255)
                                                                            gCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAg                                                                      <  1:3745659/150‑1 (MQ=255)
                                                                              cAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGt                                                                    >  1:2398462/1‑150 (MQ=255)
                                                                                 cGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTggg                                                                 >  1:4511533/1‑150 (MQ=255)
                                                                                  gggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGc                                                                >  1:2831105/1‑150 (MQ=255)
                                                                                  gggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGc                                                                >  2:352453/1‑150 (MQ=255)
                                                                                   ggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCa                                                               <  1:1157305/150‑1 (MQ=255)
                                                                                   ggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCa                                                               <  2:4330369/150‑1 (MQ=255)
                                                                                    gAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAt                                                              >  2:1047075/1‑150 (MQ=255)
                                                                                     aaTTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAtt                                                             >  2:2039070/1‑150 (MQ=255)
                                                                                      aTTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAttt                                                            <  2:1718088/150‑1 (MQ=255)
                                                                                       ttCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAtttt                                                           <  1:1100803/150‑1 (MQ=255)
                                                                                        tcgtcgTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTtc                                                          <  2:423535/150‑1 (MQ=255)
                                                                                           tcgtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTcc                                                       <  2:4380824/150‑1 (MQ=255)
                                                                                            cgtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCg                                                      >  1:4710488/1‑150 (MQ=255)
                                                                                            cgtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCg                                                      >  1:693450/1‑150 (MQ=255)
                                                                                                 tGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGaa                                                 <  2:4196164/150‑1 (MQ=255)
                                                                                                  gCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGaaa                                                >  1:4653516/1‑150 (MQ=255)
                                                                                                  gCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGaaa                                                >  2:2635841/1‑150 (MQ=255)
                                                                                                      tCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCg                                            >  2:3731912/1‑150 (MQ=255)
                                                                                                      tCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCAGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGCCAGTTTCTCAGTCGAAATGCg                                            >  2:5079769/1‑150 (MQ=255)
                                                                                                         tGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGaa                                         <  2:2125201/150‑1 (MQ=255)
                                                                                                         tGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGaa                                         <  1:1625056/150‑1 (MQ=255)
                                                                                                            cAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATg                                      <  2:2984246/150‑1 (MQ=255)
                                                                                                            cAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATg                                      <  2:4940833/150‑1 (MQ=255)
                                                                                                            cAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATg                                      <  2:4632789/150‑1 (MQ=255)
                                                                                                              gTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCt                                    >  1:579987/1‑150 (MQ=255)
                                                                                                                 cTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCa                                 <  1:2461746/150‑1 (MQ=255)
                                                                                                                  ttCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCag                                <  1:4516231/150‑1 (MQ=255)
                                                                                                                   tCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagc                               >  1:112925/1‑150 (MQ=255)
                                                                                                                     aTCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcga                             >  2:2196465/1‑150 (MQ=255)
                                                                                                                     aTCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcga                             <  1:1534001/150‑1 (MQ=255)
                                                                                                                       ccgccgCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcgagc                           <  2:1305035/150‑1 (MQ=255)
                                                                                                                        cgccgcCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcgagcg                          <  2:287608/150‑1 (MQ=255)
                                                                                                                         gccgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGt                         >  2:3904477/1‑150 (MQ=255)
                                                                                                                           cgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTg                       <  2:5089450/150‑1 (MQ=255)
                                                                                                                            gccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGa                      >  2:3400651/1‑150 (MQ=255)
                                                                                                                               tGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGACATGCGGAAATGCTCCAGCGAGCGTTGAGtt                   >  2:2741702/1‑150 (MQ=255)
                                                                                                                                  cGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTtgt                >  2:2571515/1‑150 (MQ=255)
                                                                                                                                    aaTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgc              >  2:2048756/1‑150 (MQ=255)
                                                                                                                                    aaTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgc              >  1:4582798/1‑150 (MQ=255)
                                                                                                                                      tGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCCGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgcgc            >  1:4039661/1‑150 (MQ=255)
                                                                                                                                         cgctcgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGcccc         >  2:2240547/1‑150 (MQ=255)
                                                                                                                                            tcgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTACGCCCcaca      >  1:1487299/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTT  >  CP009273/1680495‑1680788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑