BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,698,256	C→T	6.6%	A104T (GCG→ACG)	ydgJ ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,698,256	0	C	T	6.6%	1116.0 / 42.4	330	A104T (GCG→ACG)	ydgJ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (156/152); new base T (13/9); total (169/161)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

GTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAG  >  CP009273/1698107‑1698405

     aaTGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGTGCTtgtg                                                                                                                                                  <  1:4528639/150‑1 (MQ=255)

       tGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGAc                                                                                                                                                >  1:4285190/1‑150 (MQ=255)

         agaCTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAg                                                                                                                                              >  1:4187147/1‑150 (MQ=255)

          gaCTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAgt                                                                                                                                             <  1:1013393/150‑1 (MQ=255)

          gaCTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAgt                                                                                                                                             <  1:2479860/150‑1 (MQ=255)

                aaGTAAGCAACTTCACCCATCAAGCCATCCGCGAGTAAACCATTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACg                                                                                                                                       <  2:2112076/150‑1 (MQ=255)

                  gTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGt                                                                                                                                     >  1:1821682/1‑150 (MQ=255)

                    aaGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTaa                                                                                                                                   >  1:264693/1‑150 (MQ=255)

                      gCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAg                                                                                                                                 <  1:571367/150‑1 (MQ=255)

                      gCAACTTAACCCAGCACGCCTTGCGCGAGTATACCTTTTAGCGTCAAGAAATCGCTATCCCCGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTTAAg                                                                                                                                 <  1:3855253/150‑1 (MQ=255)

                       cAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAgg                                                                                                                                <  1:515042/150‑1 (MQ=255)

                        aaCTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAggg                                                                                                                               <  2:11816/150‑1 (MQ=255)

                         aCTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGt                                                                                                                              <  1:3472425/150‑1 (MQ=255)

                          cTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGtt                                                                                                                             >  1:4363788/1‑150 (MQ=255)

                          cTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGtt                                                                                                                             >  2:4219685/1‑150 (MQ=255)

                           ttCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGttt                                                                                                                            <  2:851057/150‑1 (MQ=255)

                             cACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTAt                                                                                                                          <  2:3525337/150‑1 (MQ=255)

                              aCCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATc                                                                                                                         <  2:1064167/150‑1 (MQ=255)

                               cccAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCa                                                                                                                        <  2:2191186/150‑1 (MQ=255)

                                 cAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAAc                                                                                                                      <  1:2858068/150‑1 (MQ=255)

                                 cAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAAc                                                                                                                      >  1:871975/1‑150 (MQ=255)

                                  aGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGTACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACg                                                                                                                     >  1:716175/1‑150 (MQ=255)

                                  aGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACg                                                                                                                     <  2:3311553/150‑1 (MQ=255)

                                      cGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGACATCGCTATCCCAGCGACGGTTCTGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCGCGCGTTTGTGACAGTGTCACGGTCAAGGGTGTATCAACGACca                                                                                                                 >  1:4447389/1‑150 (MQ=255)

                                      cGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACca                                                                                                                 <  1:2598863/150‑1 (MQ=255)

                                        ccTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACcaca                                                                                                               <  2:906093/150‑1 (MQ=255)

                                         cTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACAt                                                                                                              >  2:1679178/1‑150 (MQ=255)

                                            ccGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGAGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGtt                                                                                                           <  1:244745/150‑1 (MQ=255)

                                               cgAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTAc                                                                                                        >  1:13074/1‑150 (MQ=255)

                                                  gTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCg                                                                                                     >  1:3197286/1‑150 (MQ=255)

                                                     aaCCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCt                                                                                                  >  1:2995176/1‑150 (MQ=255)

                                                      aCCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGTCAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTc                                                                                                 <  2:1826819/150‑1 (MQ=255)

                                                      aCCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTATTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTc                                                                                                 >  2:432121/1‑150 (MQ=255)

                                                        cTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCaa                                                                                               >  2:4484707/1‑150 (MQ=255)

                                                        cTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCaa                                                                                               >  2:3274565/1‑150 (MQ=255)

                                                         ttttAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAg                                                                                              <  2:3431805/150‑1 (MQ=255)

                                                          tttAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAgc                                                                                             >  2:1964026/1‑150 (MQ=255)

                                                          tttAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAgc                                                                                             <  1:3944910/150‑1 (MQ=255)

                                                          tttAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAgc                                                                                             <  2:2824334/150‑1 (MQ=255)

                                                            tAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAATGGGTTTATCAACGACCACATGTTTACCCGCCTCAAgcgc                                                                                           >  1:4025417/1‑150 (MQ=255)

                                                              gCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGcgccg                                                                                         <  2:4187064/150‑1 (MQ=255)

                                                                  cAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCttt                                                                                     <  2:562625/150‑1 (MQ=255)

                                                                  cAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCttt                                                                                     <  1:2520861/150‑1 (MQ=255)

                                                                   aaGAACTCGCGATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTg                                                                                    <  2:5004706/150‑1 (MQ=255)

                                                                    aGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGTACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTgg                                                                                   <  2:716175/150‑1 (MQ=255)

                                                                      aaaTCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCt                                                                                 >  1:168551/1‑150 (MQ=255)

                                                                      aaaTCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCt                                                                                 >  2:2339068/1‑150 (MQ=255)

                                                                       aaTCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTa                                                                                <  1:1471368/150‑1 (MQ=255)

                                                                         tCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAAc                                                                              >  1:2350363/1‑150 (MQ=255)

                                                                           gCTATCCCCGCGACGGTTATGGAATACAGACAGCACACGCCCCCGGCTTTTTGCCAGCGCATCCCGCTCTCGCGCTTGTGACAGTGTCACGGTAACGGGTTTATCAACGACCACAGGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACgg                                                                            <  1:967761/150‑1 (MQ=255)

                                                                             tATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGa                                                                          >  2:1978065/1‑150 (MQ=255)

                                                                             tATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACgggc                                                                          >  2:2363583/1‑149 (MQ=255)

                                                                               tCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGaaa                                                                        <  1:4787535/150‑1 (MQ=255)

                                                                               tCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGaaa                                                                        <  1:2035934/150‑1 (MQ=255)

                                                                                 ccAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATg                                                                      <  1:1056148/150‑1 (MQ=255)

                                                                                  cAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATgg                                                                     >  1:603152/1‑150 (MQ=255)

                                                                                    gCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGt                                                                   >  2:1396270/1‑150 (MQ=255)

                                                                                      gACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTAt                                                                 <  2:2744066/150‑1 (MQ=255)

                                                                                      gACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTAt                                                                 >  1:4251456/1‑150 (MQ=255)

                                                                                      gACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTAt                                                                 >  2:4664609/1‑150 (MQ=255)

                                                                                      gACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTAt                                                                 <  2:3163067/150‑1 (MQ=255)

                                                                                       aCGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATc                                                                >  2:1607978/1‑150 (MQ=255)

                                                                                        cGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCg                                                               <  1:377277/150‑1 (MQ=255)

                                                                                         ggTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGt                                                              >  2:2590446/1‑150 (MQ=255)

                                                                                          gTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGtt                                                             >  1:3648858/1‑150 (MQ=255)

                                                                                            tATGGAATACAGACAGCACACGCCCCAGGCTTTTTGGCAGTGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTgg                                                           <  2:3028722/150‑1 (MQ=255)

                                                                                            tATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTgg                                                           <  2:1073837/150‑1 (MQ=255)

                                                                                             aTGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTggg                                                          >  2:2717144/1‑150 (MQ=255)

                                                                                               ggAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGgtg                                                        >  1:4715195/1‑150 (MQ=255)

                                                                                                gAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGgtgt                                                       >  2:1461572/1‑150 (MQ=255)

                                                                                                gAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGgtgt                                                       >  1:351672/1‑150 (MQ=255)

                                                                                                 aaTACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTa                                                      <  1:2127973/150‑1 (MQ=255)

                                                                                                  aTACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAg                                                     >  1:5047841/1‑150 (MQ=255)

                                                                                                      agacagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGTTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAAt                                                 <  2:3931863/150‑1 (MQ=255)

                                                                                                       gacagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATg                                                <  1:1922388/150‑1 (MQ=255)

                                                                                                               acGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAg                                        >  2:1858442/1‑150 (MQ=255)

                                                                                                                  ccccAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTACAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGACATGGGTATCGTTGGGGGTAGGAATGACAATCAGGTc                                     >  2:2250742/1‑150 (MQ=255)

                                                                                                                    ccAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTa                                   <  2:3067611/150‑1 (MQ=255)

                                                                                                                    ccAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTa                                   >  1:4529243/1‑150 (MQ=255)

                                                                                                                       ggCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGt                                >  2:2533770/1‑150 (MQ=255)

                                                                                                                        gCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGtt                               <  1:3063369/150‑1 (MQ=255)

                                                                                                                           ttttGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg                            <  2:5148949/150‑1 (MQ=255)

                                                                                                                           ttttGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg                            <  1:4154401/150‑1 (MQ=255)

                                                                                                                              tGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATc                         <  1:3280594/150‑1 (MQ=255)

                                                                                                                               gCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCg                        <  1:355941/150‑1 (MQ=255)

                                                                                                                                   gcgcATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTaa                    <  2:815940/150‑1 (MQ=255)

                                                                                                                                     gcATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAAc                  >  2:1476446/1‑150 (MQ=255)

                                                                                                                                       aTCCAGCTCTCGCGCTTGTGACAGTGTCACGGTACAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTCTGTTGGGATCGGTAAACAg                >  2:4042222/1‑150 (MQ=255)

                                                                                                                                       aTCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAg                >  2:1095996/1‑150 (MQ=255)

                                                                                                                                         ccAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGAt              <  2:3730966/150‑1 (MQ=255)

                                                                                                                                           aGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGc            >  1:4183152/1‑150 (MQ=255)

                                                                                                                                              tctcGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTc         <  2:2897685/150‑1 (MQ=255)

                                                                                                                                               ctcGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCg        <  2:4015829/150‑1 (MQ=255)

                                                                                                                                               ctcGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCg        <  1:4660528/150‑1 (MQ=255)

                                                                                                                                                tcGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCgg       <  1:1679178/150‑1 (MQ=255)

                                                                                                                                                tcGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCgg       >  1:1141460/1‑150 (MQ=255)

                                                                                                                                                  gcgcTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCt     <  2:635864/150‑1 (MQ=255)

                                                                                                                                                    gcTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCa   <  2:4691491/150‑1 (MQ=255)

                                                                                                                                                    gcTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCa   <  1:4934005/150‑1 (MQ=255)

                                                                                                                                                    gcTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCa   <  2:36978/150‑1 (MQ=255)

GTCAAAATGAGACTCAAAGTAAGCAACTTCACCCAGCACGCCTTCCGCGAGTAAACCTTTTAGCGTCAAGAAATCGCTATCCCAGCGACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAG  >  CP009273/1698107‑1698405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`