Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,710,635 T→A 11.3% E282V (GAG→GTG)  tyrS ← tyrosyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,710,6350TA11.3% 888.6 / 79.7 298E282V (GAG→GTG) tyrStyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (119/145);  new base A (13/21);  total (132/166)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

AAGAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCA  >  CP009273/1710486‑1710784
                                                                                                                                                     |                                                                                                                                                     
aaGAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGAtct                                                                                                                                                       <  1:1266060/150‑1 (MQ=255)
  gAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATctct                                                                                                                                                     >  2:3972258/1‑150 (MQ=255)
   aaCCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCtt                                                                                                                                                    <  1:4554956/150‑1 (MQ=255)
    aCCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTc                                                                                                                                                   <  2:4253958/150‑1 (MQ=255)
     ccGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCa                                                                                                                                                  <  1:2029904/150‑1 (MQ=255)
       gCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAAt                                                                                                                                                <  1:1003937/150‑1 (MQ=255)
         tGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGc                                                                                                                                              >  2:2011771/1‑150 (MQ=255)
                 cATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAg                                                                                                                                      <  1:4516412/150‑1 (MQ=255)
                   ttCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGt                                                                                                                                    <  1:3085630/150‑1 (MQ=255)
                     cGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTga                                                                                                                                  <  1:4277641/150‑1 (MQ=255)
                     cGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTga                                                                                                                                  <  1:1843621/150‑1 (MQ=255)
                      ggTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTgaa                                                                                                                                 >  2:72504/1‑150 (MQ=255)
                        tAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTgaaga                                                                                                                               >  1:793228/1‑150 (MQ=255)
                           tACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACt                                                                                                                            >  1:2368769/1‑150 (MQ=255)
                           tACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACt                                                                                                                            >  1:1646048/1‑150 (MQ=255)
                             cGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTc                                                                                                                          >  1:3811112/1‑150 (MQ=255)
                             cGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTc                                                                                                                          <  2:2539571/150‑1 (MQ=255)
                             cGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTc                                                                                                                          >  2:2469763/1‑150 (MQ=255)
                             cGTTGTGCCGCCTGCAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTc                                                                                                                          <  2:1330806/150‑1 (MQ=255)
                              gTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCa                                                                                                                         >  2:902080/1‑150 (MQ=255)
                               ttttGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAg                                                                                                                        <  2:4575292/150‑1 (MQ=255)
                               ttttGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAg                                                                                                                        >  2:3585763/1‑150 (MQ=255)
                                  tGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGaa                                                                                                                     >  1:4714595/1‑150 (MQ=255)
                                  tGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGaa                                                                                                                     >  2:347724/1‑150 (MQ=255)
                                       ccTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGt                                                                                                                <  1:2565346/150‑1 (MQ=255)
                                           tAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAAc                                                                                                            >  2:171852/1‑150 (MQ=255)
                                           tAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAAc                                                                                                            <  1:3804739/150‑1 (MQ=255)
                                            aaaCCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACg                                                                                                           <  2:1845581/150‑1 (MQ=255)
                                               ccTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCg                                                                                                        <  2:3868102/150‑1 (MQ=255)
                                               ccTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCg                                                                                                        <  1:4400585/150‑1 (MQ=255)
                                                  tcttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCa                                                                                                     <  2:1781356/150‑1 (MQ=255)
                                                  tcttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCa                                                                                                     >  2:2837002/1‑150 (MQ=255)
                                                   cttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCAt                                                                                                    <  2:2801532/150‑1 (MQ=255)
                                                    ttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATc                                                                                                   <  1:3877933/150‑1 (MQ=255)
                                                     tcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATcc                                                                                                  <  1:331713/150‑1 (MQ=255)
                                                     tcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATcc                                                                                                  <  1:1601125/150‑1 (MQ=255)
                                                     tcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATca                                                                                                  <  1:2975616/150‑2 (MQ=255)
                                                      cACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCg                                                                                                 <  2:3552093/150‑1 (MQ=255)
                                                      cACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCg                                                                                                 <  2:221043/150‑1 (MQ=255)
                                                         cGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAg                                                                                              <  1:4857124/150‑1 (MQ=255)
                                                         cGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCACTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAg                                                                                              <  2:1090794/150‑1 (MQ=255)
                                                          gTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAgt                                                                                             <  1:2204110/150‑1 (MQ=255)
                                                           tGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAgtg                                                                                            <  2:5157206/150‑1 (MQ=255)
                                                             aaCCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGtt                                                                                          <  2:4963132/150‑1 (MQ=255)
                                                              aCCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTg                                                                                         <  1:4632100/150‑1 (MQ=255)
                                                              aCCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTg                                                                                         <  1:4462965/150‑1 (MQ=255)
                                                                 aGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATc                                                                                      <  2:1976556/150‑1 (MQ=255)
                                                                   aCGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCa                                                                                    <  2:4773483/150‑1 (MQ=255)
                                                                   aCGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCa                                                                                    <  2:5049387/150‑1 (MQ=255)
                                                                     gAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGa                                                                                  >  1:4445353/1‑150 (MQ=255)
                                                                     gAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGa                                                                                  <  2:3642487/150‑1 (MQ=255)
                                                                        tCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACt                                                                               >  2:2281369/1‑150 (MQ=255)
                                                                            cTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTa                                                                           <  2:4891662/150‑1 (MQ=255)
                                                                              gCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGa                                                                         <  2:3100613/150‑1 (MQ=255)
                                                                               cTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGaa                                                                        >  2:4627381/1‑150 (MQ=255)
                                                                                 ccGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAAtt                                                                      >  2:3387962/1‑150 (MQ=255)
                                                                                   gCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTACACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTg                                                                    >  1:4194944/1‑150 (MQ=255)
                                                                                     cAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTa                                                                  >  1:303620/1‑150 (MQ=255)
                                                                                       gTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACg                                                                <  2:5110220/150‑1 (MQ=255)
                                                                                           aTACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCt                                                            <  1:39799/150‑1 (MQ=255)
                                                                                             aCTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTgg                                                          >  2:3412367/1‑150 (MQ=255)
                                                                                               tGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGtt                                                        >  2:1419792/1‑150 (MQ=255)
                                                                                               tGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGtt                                                        <  1:2112199/150‑1 (MQ=255)
                                                                                                gggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGttt                                                       <  2:3340617/150‑1 (MQ=255)
                                                                                                    gcgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTttctt                                                   <  1:3740231/150‑1 (MQ=255)
                                                                                                     cgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTttcttc                                                  >  2:3781489/1‑150 (MQ=255)
                                                                                                     cgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTttcttc                                                  <  2:4156621/150‑1 (MQ=255)
                                                                                                      gcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCg                                                 >  1:1090576/1‑150 (MQ=255)
                                                                                                       cgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCgg                                                >  1:1139235/1‑150 (MQ=255)
                                                                                                       cgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCgg                                                <  1:3525514/150‑1 (MQ=255)
                                                                                                          tGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATc                                             <  2:3233467/150‑1 (MQ=255)
                                                                                                           gCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATcc                                            <  2:3984491/150‑1 (MQ=255)
                                                                                                           gCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATcc                                            >  1:3149750/1‑150 (MQ=255)
                                                                                                            cTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCa                                           <  1:5027336/150‑1 (MQ=255)
                                                                                                                aCCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCa                                       <  2:1381531/150‑1 (MQ=255)
                                                                                                                 ccGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAg                                      >  1:1371407/1‑150 (MQ=255)
                                                                                                                  cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGa                                     >  1:1511759/1‑150 (MQ=255)
                                                                                                                    cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                   <  2:4030518/150‑1 (MQ=255)
                                                                                                                    cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                   >  2:2972743/1‑150 (MQ=255)
                                                                                                                     tGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                  <  1:2484496/150‑1 (MQ=255)
                                                                                                                     tGTTTTTATCTTCTTCGTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTg                                  <  2:491908/150‑1 (MQ=255)
                                                                                                                        ttttATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcgc                               >  1:3586864/1‑150 (MQ=255)
                                                                                                                         tttATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgcc                              <  2:4508136/150‑1 (MQ=255)
                                                                                                                         tttATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGcgcc                              <  2:1113748/150‑1 (MQ=255)
                                                                                                                              cttcttcttCCAGGGCGTTGATCACTTCCATGCACATAAAGGTGAAGAACATCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCtt                         <  1:2312588/150‑1 (MQ=255)
                                                                                                                               ttcttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTAGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTc                        <  1:2790842/150‑1 (MQ=255)
                                                                                                                                  ttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGt                     >  1:849430/1‑150 (MQ=255)
                                                                                                                                  ttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGt                     >  1:3491481/1‑150 (MQ=255)
                                                                                                                                   tcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtt                    <  1:2299457/150‑1 (MQ=255)
                                                                                                                                   tcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtt                    <  1:1024024/150‑1 (MQ=255)
                                                                                                                                    cttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGttt                   >  2:216783/1‑150 (MQ=255)
                                                                                                                                       ccAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAc                >  2:3030374/1‑150 (MQ=255)
                                                                                                                                       ccAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAc                >  1:2461861/1‑150 (MQ=255)
                                                                                                                                             cGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAtt          >  1:1734253/1‑150 (MQ=255)
                                                                                                                                              gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAttt         >  2:1267478/1‑150 (MQ=255)
                                                                                                                                              gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAttt         <  2:1243921/150‑1 (MQ=255)
                                                                                                                                              gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAgtt         >  2:601799/1‑150 (MQ=255)
                                                                                                                                               ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTg        <  2:2308846/150‑1 (MQ=255)
                                                                                                                                                  aTCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTg     <  1:516428/150‑1 (MQ=255)
                                                                                                                                                     tctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCa  >  1:4101110/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCA  >  CP009273/1710486‑1710784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑