Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,723,809 G→T 100% E156* (GAA→TAA)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,723,8090GT98.7% 997.9 / 2.2 311E156* (GAA→TAA) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (141/166);  total (143/168)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 311 total aligned reads displayed.

ATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTC  >  CP009273/1723662‑1723955
                                                                                                                                                   |                                                                                                                                                  
aTCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTaa                                                                                                                                                  <  2:4088068/150‑1 (MQ=255)
 tCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAAt                                                                                                                                                 <  2:4057445/150‑1 (MQ=255)
  cccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATc                                                                                                                                                <  2:2924150/150‑1 (MQ=255)
     gTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAct                                                                                                                                             <  2:2274377/150‑1 (MQ=255)
         aaGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAt                                                                                                                                         >  2:2061176/1‑150 (MQ=255)
         aaGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAt                                                                                                                                         <  1:4152852/150‑1 (MQ=255)
         aaGGGTAGTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAt                                                                                                                                         <  1:3038612/150‑1 (MQ=255)
          aGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATc                                                                                                                                        >  1:1034065/1‑150 (MQ=255)
          aGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATc                                                                                                                                        <  2:2532928/150‑1 (MQ=255)
             gTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGa                                                                                                                                     >  2:3064639/1‑150 (MQ=255)
              tATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGAt                                                                                                                                    >  1:539589/1‑150 (MQ=255)
                   ccGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGa                                                                                                                               <  2:4280416/150‑1 (MQ=255)
                    cGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAc                                                                                                                              >  1:871208/1‑150 (MQ=255)
                     gATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                             >  1:2566742/1‑150 (MQ=255)
                     gATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGAcc                                                                                                                             <  2:5151448/150‑1 (MQ=255)
                     gATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATAGTCTGATCACCACACCCTAATCACTCTCTCTGATGCTGAcc                                                                                                                             >  2:3768375/1‑150 (MQ=255)
                      aTGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCt                                                                                                                            <  1:4780323/150‑1 (MQ=255)
                          aCGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTACTCACGCTATCTGATGCTGACCTCCcg                                                                                                                        >  1:5114879/1‑150 (MQ=255)
                          aCGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcg                                                                                                                        >  1:734619/1‑150 (MQ=255)
                           cggcggcggCGCGGCGACACGGAAGTCAATCGTCGCGTAGGGATCCGTACGGCCGATACGCCTGCACAGGAACGCAGCAAACGCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgc                                                                                                                       <  2:2645030/150‑1 (MQ=255)
                           cggcggcggCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgc                                                                                                                       <  1:3576808/150‑1 (MQ=255)
                             gcggcggcgCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgcgc                                                                                                                     <  2:3585639/150‑1 (MQ=255)
                               ggcggcgCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgcgcgc                                                                                                                   >  2:2692892/1‑150 (MQ=255)
                               ggcggcgCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCcgcgcgc                                                                                                                   <  1:2780732/150‑1 (MQ=255)
                                      cggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAAc                                                                                                            <  1:3339857/150‑1 (MQ=255)
                                       ggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                           <  1:4540092/150‑1 (MQ=255)
                                        gcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGc                                                                                                          <  2:3109684/150‑1 (MQ=255)
                                         cgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                         >  1:2490025/1‑150 (MQ=255)
                                           aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAc                                                                                                       >  2:1322683/1‑150 (MQ=255)
                                           aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAc                                                                                                       <  1:4362050/150‑1 (MQ=255)
                                            aaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcg                                                                                                      <  2:3470462/150‑1 (MQ=255)
                                            aaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcg                                                                                                      >  2:729785/1‑150 (MQ=255)
                                                   gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTc                                                                                               <  2:4526307/150‑1 (MQ=255)
                                                   gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTc                                                                                               <  1:690855/150‑1 (MQ=255)
                                                    tCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCg                                                                                              <  2:542723/150‑1 (MQ=255)
                                                     cAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGa                                                                                             <  2:2713165/150‑1 (MQ=255)
                                                        tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAAc                                                                                          <  2:4193728/150‑1 (MQ=255)
                                                         cTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACg                                                                                         <  1:1821458/150‑1 (MQ=255)
                                                             gcgTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTaa                                                                                     <  1:2098203/150‑1 (MQ=255)
                                                                tAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAattn                                                                                  <  1:1657162/150‑2 (MQ=255)
                                                                tAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAatta                                                                                  >  2:4574752/1‑150 (MQ=255)
                                                                  gggATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAattatt                                                                                >  2:1369715/1‑150 (MQ=255)
                                                                      tCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatg                                                                            <  2:777796/150‑1 (MQ=255)
                                                                       ccGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGACACGGGACTTATtgatga                                                                           >  1:2662413/1‑150 (MQ=255)
                                                                       ccGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                           >  1:4973102/1‑150 (MQ=255)
                                                                        cGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                          >  1:785244/1‑150 (MQ=255)
                                                                        cGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                          >  2:3850960/1‑150 (MQ=255)
                                                                           aCTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTc                                                                       <  2:1878888/150‑1 (MQ=255)
                                                                            cTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTcc                                                                      >  1:3980055/1‑150 (MQ=255)
                                                                             tGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTACTTATTGATGAAGTCCa                                                                     >  1:3286583/1‑150 (MQ=255)
                                                                              ggCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAc                                                                    >  2:1590780/1‑150 (MQ=255)
                                                                               gCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCAcg                                                                   <  2:175801/150‑1 (MQ=255)
                                                                                 gATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTACTTATTGATGAAGTCCAcgcg                                                                 >  2:3629771/1‑150 (MQ=255)
                                                                                   tACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGt                                                               >  1:4274776/1‑150 (MQ=255)
                                                                                    aCGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTa                                                              >  2:2308972/1‑150 (MQ=255)
                                                                                     cGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGACGTCCACGCGGTAg                                                             <  1:722068/150‑1 (MQ=255)
                                                                                     cGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAg                                                             <  1:1537051/150‑1 (MQ=255)
                                                                                         tGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCggg                                                         <  1:4943710/150‑1 (MQ=255)
                                                                                           cacaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCa                                                       >  2:3558474/1‑150 (MQ=255)
                                                                                           cacaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCa                                                       <  1:2237771/150‑1 (MQ=255)
                                                                                            acaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAg                                                      >  2:3828754/1‑150 (MQ=255)
                                                                                              aGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTa                                                    >  2:5026507/1‑150 (MQ=255)
                                                                                               ggAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                   <  1:2694592/150‑1 (MQ=255)
                                                                                               ggAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaa                                                   >  2:1497109/1‑150 (MQ=255)
                                                                                                gAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTaaa                                                  >  2:4993393/1‑150 (MQ=255)
                                                                                                  aCGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACg                                                <  2:2835389/150‑1 (MQ=255)
                                                                                                    gcagcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtg                                              <  1:2406034/150‑1 (MQ=255)
                                                                                                    gcagcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtg                                              >  2:3097512/1‑150 (MQ=255)
                                                                                                     cagcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtgg                                             >  1:3798873/1‑150 (MQ=255)
                                                                                                     cagcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCGATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtgg                                             <  2:4428709/150‑1 (MQ=255)
                                                                                                       gcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggtg                                           <  1:928787/150‑1 (MQ=255)
                                                                                                       gcaAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggta                                           <  2:2854506/150‑2 (MQ=255)
                                                                                                        caAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTgc                                          >  2:2416607/1‑150 (MQ=255)
                                                                                                         aaaCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAAGTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTgcg                                         >  2:2370860/1‑150 (MQ=255)
                                                                                                           aCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCa                                       >  1:1882158/1‑150 (MQ=255)
                                                                                                              cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCt                                    >  1:1976882/1‑150 (MQ=255)
                                                                                                                cccGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAAGTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTgg                                  >  2:1085355/1‑150 (MQ=255)
                                                                                                                  cGTAATCCGCCGGATATTCTGATCTCCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCg                                <  2:2213795/150‑1 (MQ=255)
                                                                                                                     aaTCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTCATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTa                             >  2:839051/1‑150 (MQ=255)
                                                                                                                     aaTCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTa                             >  1:1744100/1‑150 (MQ=255)
                                                                                                                      aTCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTaa                            <  2:1211249/150‑1 (MQ=255)
                                                                                                                      aTCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTaa                            >  1:4640097/1‑150 (MQ=255)
                                                                                                                         cgccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                         >  2:3310000/1‑150 (MQ=255)
                                                                                                                          gccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCt                        <  2:371534/150‑1 (MQ=255)
                                                                                                                          gccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCt                        <  1:3558474/150‑1 (MQ=255)
                                                                                                                           ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGATACTGTAATTCTTTATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTg                       <  2:3575976/150‑1 (MQ=255)
                                                                                                                              gATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAg                    <  2:669406/150‑1 (MQ=255)
                                                                                                                                tatTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCg                  <  1:3704428/150‑1 (MQ=255)
                                                                                                                                   tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCt               <  2:2566742/150‑1 (MQ=255)
                                                                                                                                     tGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCg             >  2:4915969/1‑150 (MQ=255)
                                                                                                                                       aTCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAt           <  1:2864837/150‑1 (MQ=255)
                                                                                                                                         caccacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgc         >  1:2726534/1‑150 (MQ=255)
                                                                                                                                         caccacACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgc         >  1:4870173/1‑150 (MQ=255)
                                                                                                                                          accacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcg        <  1:1065253/150‑1 (MQ=255)
                                                                                                                                            cacaCCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgct      >  1:3594459/1‑150 (MQ=255)
                                                                                                                                             acacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctg     <  2:4060053/150‑1 (MQ=255)
                                                                                                                                              cacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctgc    >  1:3462761/1‑150 (MQ=255)
                                                                                                                                               acCCTACTCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctgct   <  1:3516704/150‑1 (MQ=255)
                                                                                                                                               acCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctgct   <  2:630810/150‑1 (MQ=255)
                                                                                                                                               acCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgctgct   >  2:3164141/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTC  >  CP009273/1723662‑1723955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑