Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,754,950 T→A 100% I367F (ATT→TTT)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,754,9500TA100.0% 965.9 / NA 299I367F (ATT→TTT) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (142/157);  total (142/157)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 299 total aligned reads displayed.

GACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGT  >  CP009273/1754803‑1755096
                                                                                                                                                   |                                                                                                                                                  
gACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                                  >  2:277739/1‑150 (MQ=255)
gACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                                  <  1:1675807/150‑1 (MQ=255)
gACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                                  <  1:5034954/150‑1 (MQ=255)
gACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                                  >  1:549515/1‑150 (MQ=255)
gACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAAc                                                                                                                                                  <  1:729994/150‑1 (MQ=255)
 aCCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACg                                                                                                                                                 <  2:2791799/150‑1 (MQ=255)
   cGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcc                                                                                                                                               >  1:4877776/1‑150 (MQ=255)
   cGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcc                                                                                                                                               >  2:1307922/1‑150 (MQ=255)
   cGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcc                                                                                                                                               >  2:2299343/1‑150 (MQ=255)
    gATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGccc                                                                                                                                              >  1:113678/1‑150 (MQ=255)
    gATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGccc                                                                                                                                              <  2:4723988/150‑1 (MQ=255)
    gATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGccc                                                                                                                                              >  2:2450013/1‑150 (MQ=255)
     aTTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGcccc                                                                                                                                             <  1:853981/150‑1 (MQ=255)
        cGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCAcc                                                                                                                                          <  1:3347883/150‑1 (MQ=255)
         gggCCAGCACCTGCTGTTTAAGCCCCTCATCACTCAGTTCTTCCGTCAGTTCGGCAGCGAAGGCGTAAAAGATCATATGCTGGGCATCATGCTGATTGATCCCGCGCGAGAGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCg                                                                                                                                         >  1:1012234/1‑150 (MQ=255)
         gggCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCg                                                                                                                                         <  1:3085219/150‑1 (MQ=255)
          ggCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGt                                                                                                                                        <  1:2334219/150‑1 (MQ=255)
          ggCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGt                                                                                                                                        >  2:875872/1‑150 (MQ=255)
           gCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTc                                                                                                                                       <  2:3799145/150‑1 (MQ=255)
            ccAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTcg                                                                                                                                      <  1:2260389/150‑1 (MQ=255)
                cACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGcc                                                                                                                                  >  2:4018219/1‑150 (MQ=255)
                cACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGcc                                                                                                                                  <  2:2837662/150‑1 (MQ=255)
                     gctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGc                                                                                                                             >  1:3942137/1‑150 (MQ=255)
                      ctgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCt                                                                                                                            <  2:565706/150‑1 (MQ=255)
                       tgTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTg                                                                                                                           >  2:2146504/1‑150 (MQ=255)
                              gCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTca                                                                                                                    >  2:2596764/1‑150 (MQ=255)
                               ccccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTcac                                                                                                                   <  2:1563109/150‑1 (MQ=255)
                                cccTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTcaca                                                                                                                  <  1:3405449/150‑1 (MQ=255)
                                  cTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatc                                                                                                                <  1:4755528/150‑1 (MQ=255)
                                  cTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCAcatc                                                                                                                >  2:3551241/1‑150 (MQ=255)
                                     atcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACatcatc                                                                                                             >  2:1943754/1‑150 (MQ=255)
                                      tcaCGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCt                                                                                                            <  1:2538932/150‑1 (MQ=255)
                                            aGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAg                                                                                                      >  1:1861596/1‑150 (MQ=255)
                                            aGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATAATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAg                                                                                                      >  1:4631331/1‑150 (MQ=255)
                                               gCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGAtt                                                                                                   <  2:225706/150‑1 (MQ=255)
                                                cTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGAttt                                                                                                  >  1:5085950/1‑150 (MQ=255)
                                                 ttCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTc                                                                                                 >  2:5148485/1‑150 (MQ=255)
                                                  tCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTcc                                                                                                <  2:1795363/150‑1 (MQ=255)
                                                   ccGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCa                                                                                               <  2:1654953/150‑1 (MQ=255)
                                                   ccGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCa                                                                                               <  2:2896273/150‑1 (MQ=255)
                                                    cGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAg                                                                                              >  1:383372/1‑150 (MQ=255)
                                                       cAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTg                                                                                           <  2:59397/150‑1 (MQ=255)
                                                        aGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                          >  1:3537760/1‑150 (MQ=255)
                                                        aGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                          <  1:4485891/150‑1 (MQ=255)
                                                        aGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGc                                                                                          <  1:4486306/150‑1 (MQ=255)
                                                         gTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGCGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCg                                                                                         <  1:2621749/150‑1 (MQ=255)
                                                           tCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGt                                                                                       <  2:2636734/150‑1 (MQ=255)
                                                           tCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGt                                                                                       >  1:4115520/1‑150 (MQ=255)
                                                            cGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGtt                                                                                      <  2:5045823/150‑1 (MQ=255)
                                                            cGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGtt                                                                                      <  2:4077643/150‑1 (MQ=255)
                                                            cGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGtt                                                                                      >  2:3093703/1‑150 (MQ=255)
                                                               cAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCg                                                                                   <  1:908735/150‑1 (MQ=255)
                                                                aGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGt                                                                                  >  2:635350/1‑150 (MQ=255)
                                                                 gCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTa                                                                                 <  1:1904902/150‑1 (MQ=255)
                                                                  cGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTAt                                                                                <  2:3573379/150‑1 (MQ=255)
                                                                         gTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTc                                                                         >  1:2582714/1‑150 (MQ=255)
                                                                          tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTcc                                                                        <  1:362114/150‑1 (MQ=255)
                                                                          tAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTcc                                                                        <  2:1574580/150‑1 (MQ=255)
                                                                              tGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCa                                                                    >  1:2070433/1‑150 (MQ=255)
                                                                              tGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCa                                                                    <  2:1340087/150‑1 (MQ=255)
                                                                                atcatcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGt                                                                  >  1:1054640/1‑150 (MQ=255)
                                                                                 tcatcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGtt                                                                 <  2:2069230/150‑1 (MQ=255)
                                                                                  catcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGttt                                                                >  2:2583424/1‑150 (MQ=255)
                                                                                  catcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGttt                                                                <  1:3295639/150‑1 (MQ=255)
                                                                                  catcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGttt                                                                >  1:4253738/1‑150 (MQ=255)
                                                                                   atcTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTg                                                               >  2:3191681/1‑150 (MQ=255)
                                                                                      tgctgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGccc                                                            >  2:2705391/1‑150 (MQ=255)
                                                                                         tgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATc                                                         >  2:1165236/1‑150 (MQ=255)
                                                                                         tgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATc                                                         >  2:2371741/1‑150 (MQ=255)
                                                                                         tgGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATc                                                         >  1:4903303/1‑150 (MQ=255)
                                                                                          gggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                        >  2:784601/1‑150 (MQ=255)
                                                                                          gggCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATca                                                        >  1:1535598/1‑150 (MQ=255)
                                                                                             cATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATcagca                                                     >  1:3451098/1‑150 (MQ=255)
                                                                                             cATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATcagaa                                                     <  2:390518/150‑3 (MQ=255)
                                                                                              aTCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATcagcag                                                    >  1:2751439/1‑150 (MQ=255)
                                                                                                    ctgATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgtt                                              >  1:4111814/1‑150 (MQ=255)
                                                                                                      gattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttgt                                            <  2:1628794/150‑1 (MQ=255)
                                                                                                      gattgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttgt                                            <  2:1072208/150‑1 (MQ=255)
                                                                                                       attgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttgtt                                           <  1:3760007/150‑1 (MQ=255)
                                                                                                       attgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttgtt                                           <  2:93765/150‑1 (MQ=255)
                                                                                                       attgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttgtt                                           <  2:3983966/150‑1 (MQ=255)
                                                                                                        ttgatCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGAttgttgttg                                          <  1:2740178/150‑1 (MQ=255)
                                                                                                             cccGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCAt                                     >  1:3930247/1‑150 (MQ=255)
                                                                                                             cccGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCAt                                     <  1:2379159/150‑1 (MQ=255)
                                                                                                             cccGCGCGAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCAt                                     >  1:4404188/1‑150 (MQ=255)
                                                                                                               cgcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCt                                   <  2:449120/150‑1 (MQ=255)
                                                                                                               cgcgcgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCt                                   >  1:2906170/1‑150 (MQ=255)
                                                                                                                   cgAGCGCAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGAcc                               >  2:2182949/1‑150 (MQ=255)
                                                                                                                      gcgcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATc                            <  2:4160059/150‑1 (MQ=255)
                                                                                                                       cggaGATAGAATATCTGTTCATCATCAAATCGCATCACAGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACGTCCGACAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATcc                           <  2:4828822/147‑1 (MQ=255)
                                                                                                                        gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCg                          <  2:4877875/150‑1 (MQ=255)
                                                                                                                        gcAGATAGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCg                          <  1:126014/150‑1 (MQ=255)
                                                                                                                              aGAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGa                    >  1:3721440/1‑150 (MQ=255)
                                                                                                                               gAATATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGAt                   <  2:4318564/150‑1 (MQ=255)
                                                                                                                                aaTATCTGTTCATCATCAAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATg                  >  1:3137768/1‑150 (MQ=255)
                                                                                                                                          catcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTgcg        >  2:2885246/1‑150 (MQ=255)
                                                                                                                                            tcatcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGcgcg      >  1:681767/1‑150 (MQ=255)
                                                                                                                                              atcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGAc    >  1:2930994/1‑150 (MQ=255)
                                                                                                                                              atcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGAc    >  1:4589729/1‑150 (MQ=255)
                                                                                                                                              atcaAAACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGAc    >  1:1331483/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGAATATCTGTTCATCATCAATACGCCCCACCGTCGCGCCGTGGCTGCATTTCACATCATCTGCATAGATTTCCAGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGT  >  CP009273/1754803‑1755096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑