Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,755,150 A→T 100% L300* (TTG→TAG)  sufD ← component of SufBCD Fe‑S cluster assembly scaffold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,755,1500AT95.1% 950.9 / 26.0 301L300* (TTG→TAG) sufDcomponent of SufBCD Fe‑S cluster assembly scaffold
Reads supporting (aligned to +/- strand):  ref base A (6/9);  new base T (151/135);  total (157/144)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 301 total aligned reads displayed.

AGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACT  >  CP009273/1755003‑1755296
                                                                                                                                                   |                                                                                                                                                  
aGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTAc                                                                                                                                                  >  1:3782241/1‑150 (MQ=255)
 gCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATACGTTTTGATGTCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACt                                                                                                                                                 >  1:1745385/1‑150 (MQ=255)
    gCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTc                                                                                                                                              >  1:1901351/1‑150 (MQ=255)
     cGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCg                                                                                                                                             >  2:2050426/1‑150 (MQ=255)
      ggTTTCGGATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCgg                                                                                                                                            <  2:2418522/150‑1 (MQ=255)
         ttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTg                                                                                                                                         >  1:2956004/1‑150 (MQ=255)
         ttCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTg                                                                                                                                         <  2:2871/150‑1 (MQ=255)
          tCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGt                                                                                                                                        <  1:3594819/150‑1 (MQ=255)
           cGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGtt                                                                                                                                       <  1:4145940/150‑1 (MQ=255)
           cGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGtt                                                                                                                                       <  1:746737/150‑1 (MQ=255)
             tATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTAc                                                                                                                                     <  2:2070433/150‑1 (MQ=255)
             tATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTAc                                                                                                                                     >  2:3501526/1‑150 (MQ=255)
             tATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAAGACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTAc                                                                                                                                     >  2:1155130/1‑150 (MQ=255)
                      ccGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCtt                                                                                                                            >  2:4261912/1‑150 (MQ=255)
                       cGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCttt                                                                                                                           <  2:2353295/150‑1 (MQ=255)
                       cGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCttt                                                                                                                           >  2:3858573/1‑150 (MQ=255)
                       cGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCttt                                                                                                                           <  1:2705391/150‑1 (MQ=255)
                          cAGTTTGCCCATCAGCAGATTGGTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTAtt                                                                                                                        <  2:4819682/150‑1 (MQ=255)
                           aGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATtg                                                                                                                       <  2:1326299/150‑1 (MQ=255)
                            gTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATtgt                                                                                                                      <  1:2885246/150‑1 (MQ=255)
                            gTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATtgt                                                                                                                      >  2:2267367/1‑150 (MQ=255)
                               tGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                   >  1:2827498/1‑150 (MQ=255)
                               tGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGtt                                                                                                                   <  2:4021106/150‑1 (MQ=255)
                                 cccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcc                                                                                                                 >  1:3624490/1‑150 (MQ=255)
                                  ccATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTcca                                                                                                                <  1:3697812/150‑1 (MQ=255)
                                   cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccag                                                                                                               >  1:3354108/1‑150 (MQ=255)
                                   cATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccag                                                                                                               >  2:3024986/1‑150 (MQ=255)
                                    aTCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagc                                                                                                              >  2:3627673/1‑150 (MQ=255)
                                      cagcagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTccagcca                                                                                                            >  2:443976/1‑150 (MQ=255)
                                         cagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGt                                                                                                         >  1:1849055/1‑150 (MQ=255)
                                         cagATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGt                                                                                                         >  1:2380961/1‑150 (MQ=255)
                                          agATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTa                                                                                                        >  2:3822527/1‑150 (MQ=255)
                                           gATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTAc                                                                                                       <  2:4115247/150‑1 (MQ=255)
                                            aTTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACg                                                                                                      >  2:802605/1‑150 (MQ=255)
                                            aTTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACg                                                                                                      <  1:4463151/150‑1 (MQ=255)
                                              tgttgttgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACggg                                                                                                    >  1:3224694/1‑150 (MQ=255)
                                               gttgttgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCATGTACGGGt                                                                                                   >  2:5084370/1‑150 (MQ=255)
                                                ttgttgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGACAGGTGCGGGTa                                                                                                  >  1:4821555/1‑150 (MQ=255)
                                                   ttgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATca                                                                                               <  1:2182986/150‑1 (MQ=255)
                                                    tgGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcac                                                                                              >  2:4695206/1‑150 (MQ=255)
                                                      gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcacac                                                                                            >  1:1416783/1‑150 (MQ=255)
                                                      gTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATcacac                                                                                            >  1:3135275/1‑150 (MQ=255)
                                                        cATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCacacac                                                                                          >  1:1607232/1‑150 (MQ=255)
                                                           cTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTc                                                                                       >  2:1279093/1‑150 (MQ=255)
                                                                  tCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTc                                                                                >  1:62984/1‑150 (MQ=255)
                                                                     gTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCAcc                                                                             <  1:4086939/150‑1 (MQ=255)
                                                                     gTTGTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCAcc                                                                             <  2:4404188/150‑1 (MQ=255)
                                                                       tttGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCgg                                                                           <  1:1973483/150‑1 (MQ=255)
                                                                          gATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCAt                                                                        <  1:432843/150‑1 (MQ=255)
                                                                             ggCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGc                                                                     >  2:3762521/1‑150 (MQ=255)
                                                                             ggCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGc                                                                     >  1:2778965/1‑150 (MQ=255)
                                                                             ggCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGc                                                                     >  2:2372667/1‑150 (MQ=255)
                                                                               cGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTTCGGGTATCACACACCTCGTTTTTCACCGGCATCGCCa                                                                   <  2:681743/150‑1 (MQ=255)
                                                                                 tgctgcGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAgg                                                                 >  2:2600621/1‑150 (MQ=255)
                                                                                    tgcGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTa                                                              <  2:3998619/150‑1 (MQ=255)
                                                                                    tgcGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTa                                                              >  1:4996123/1‑150 (MQ=255)
                                                                                      cgcgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTAtt                                                            <  2:3589296/150‑1 (MQ=255)
                                                                                      cgcgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTAtt                                                            <  1:536708/150‑1 (MQ=255)
                                                                                        cgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGa                                                          <  2:3942137/150‑1 (MQ=255)
                                                                                        cgACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGa                                                          <  1:2516288/150‑1 (MQ=255)
                                                                                         gACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                         <  1:2396098/150‑1 (MQ=255)
                                                                                         gACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGAt                                                         >  1:4870876/1‑150 (MQ=255)
                                                                                          aCGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATc                                                        <  1:4018219/150‑1 (MQ=255)
                                                                                               gATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAg                                                   >  1:3045633/1‑150 (MQ=255)
                                                                                               gATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAg                                                   <  2:4134758/150‑1 (MQ=255)
                                                                                                 tCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCg                                                 >  1:738861/1‑150 (MQ=255)
                                                                                                   aaaCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTg                                               >  2:2508924/1‑150 (MQ=255)
                                                                                                    aaCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGGTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGc                                              <  2:2000383/150‑1 (MQ=255)
                                                                                                     aCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCt                                             <  1:1796183/150‑1 (MQ=255)
                                                                                                     aCCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCt                                             <  2:2745938/150‑1 (MQ=255)
                                                                                                     aCCGTTAAAGACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCt                                             <  2:4903303/150‑1 (MQ=255)
                                                                                                      ccGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTg                                            <  1:1639499/150‑1 (MQ=255)
                                                                                                      ccGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTg                                            >  2:3184841/1‑150 (MQ=255)
                                                                                                       cGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGt                                           <  1:4695968/150‑1 (MQ=255)
                                                                                                           aaaTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                       >  2:2227197/1‑150 (MQ=255)
                                                                                                           aaaTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                       <  2:447580/150‑1 (MQ=255)
                                                                                                           aaaTACCGCGCGGCCTTTGTCGCTGACGAGAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTGATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                       <  2:2931032/150‑1 (MQ=255)
                                                                                                           aaaTACCGCGCGGCCTTGGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTc                                       <  1:901525/150‑1 (MQ=255)
                                                                                                            aagaCCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTAGTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCg                                      <  1:1102291/147‑1 (MQ=255)
                                                                                                            aaTACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCg                                      >  1:4541131/1‑150 (MQ=255)
                                                                                                                 cgcgcgGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCAtt                                 >  1:618988/1‑150 (MQ=255)
                                                                                                                  gcgcgGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTg                                <  1:957194/150‑1 (MQ=255)
                                                                                                                     cgGCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGt                             >  1:2333112/1‑150 (MQ=255)
                                                                                                                      ggCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGtt                            <  2:3137724/150‑1 (MQ=255)
                                                                                                                      ggCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGtt                            <  2:3385285/150‑1 (MQ=255)
                                                                                                                       gCCTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTg                           >  2:4765480/1‑150 (MQ=255)
                                                                                                                        ccTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGGTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGATTTCGCCATTGAGTTGc                          <  1:3898956/150‑1 (MQ=255)
                                                                                                                         cTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCg                         >  1:1032305/1‑150 (MQ=255)
                                                                                                                         cTTTGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGATTTACACCGGCAACGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCg                         >  2:168304/1‑150 (MQ=255)
                                                                                                                          tttGTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGt                        <  2:1041162/150‑1 (MQ=255)
                                                                                                                             gTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACt                     >  2:4325226/1‑150 (MQ=255)
                                                                                                                             gTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACt                     <  2:1861596/150‑1 (MQ=255)
                                                                                                                             gTCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACt                     <  2:4631331/150‑1 (MQ=255)
                                                                                                                              tCGCTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTg                    <  1:1273382/150‑1 (MQ=255)
                                                                                                                                 cTGACGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGgtg                 <  2:1057814/150‑1 (MQ=255)
                                                                                                                                     cGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt             >  2:1358875/1‑150 (MQ=255)
                                                                                                                                     cGATAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGtgttgt             >  1:2612035/1‑150 (MQ=255)
                                                                                                                                      gATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTtgtg            <  2:1535598/150‑1 (MQ=255)
                                                                                                                                        tAGTTTTGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTc          >  1:1340394/1‑150 (MQ=255)
                                                                                                                                          tttttGTGCTACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGt        >  1:4915660/2‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCTGCGGTTTCGTATCCACTTCCGCCAGTTTGCCCATCAGCAGATTGTTGTTGGTCATCTGACCATCCGTTTTGATGGCGTGCTGCGCGACGTTGATCAAACCGTTAAATACCGCGCGGCCTTTGTCGCTGACGATAGTTTTGTGCAACTGTCGGCTGTTACAAAAACCTTTATTGTGTTCCAGCCAGGTACGGGTATCACACACCTCGTTTTTCACCGGCATCGCCAGGCTATTGATCCGCAGCGTGCTGTTTTCGCCATTGAGTTGCGTACTGGTGTTGTGTCGTAACACT  >  CP009273/1755003‑1755296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑