Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,769,763 T→A 100% intergenic (+62/‑81) aroD → / → ydiF 3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,769,7630TA95.7% 663.8 / 12.3 206intergenic (+62/‑81)aroD/ydiF3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (3/6);  new base A (100/97);  total (103/103)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

TGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769617‑1769907
                                                                                                                                                  |                                                                                                                                                
     cGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATaaaaa                                                                                                                                          >  1:843037/1‑150 (MQ=255)
          aaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATAttt                                                                                                                                     >  2:4144793/1‑150 (MQ=255)
              aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTg                                                                                                                                 <  2:1294695/150‑1 (MQ=255)
              aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTg                                                                                                                                 <  2:2204299/150‑1 (MQ=255)
               aGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGa                                                                                                                                >  1:4635303/1‑150 (MQ=255)
                gCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAg                                                                                                                               >  2:1541014/1‑150 (MQ=255)
                 cGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGt                                                                                                                              <  1:2077984/150‑1 (MQ=255)
                  gTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGtc                                                                                                                             >  1:3692285/1‑150 (MQ=255)
                  gTCTGCGCCAGGGCAAATCTCGGTAAATGATATGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGCGAACACCCTCCCCGCCGAACTAAAAAAAATAATCAATCGTATTAAATAAAAATAAAACGTGAGtc                                                                                                                             >  1:3377379/1‑150 (MQ=255)
                     tGCGCCAGGGCAAATCTCGGTATATGATTTGCGCACGGTATTAAATATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATTTTCAATCGTATTAAATAAAAATATTTCGTGAGTctct                                                                                                                          <  1:1790947/150‑1 (MQ=255)
                     tGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTctct                                                                                                                          >  2:641549/1‑150 (MQ=255)
                      gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCtg                                                                                                                         <  2:1863955/150‑1 (MQ=255)
                       cgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCtgt                                                                                                                        >  2:4869573/1‑150 (MQ=255)
                          cAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcg                                                                                                                     >  1:4933344/1‑150 (MQ=255)
                          cAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcg                                                                                                                     >  2:2440027/1‑150 (MQ=255)
                          cAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcg                                                                                                                     >  1:849101/1‑150 (MQ=255)
                          cAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcg                                                                                                                     >  2:2981453/1‑150 (MQ=255)
                           aGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcgc                                                                                                                    >  2:2656201/1‑150 (MQ=255)
                              gCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTaa                                                                                                                 <  2:1066766/150‑1 (MQ=255)
                               cAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAt                                                                                                                <  2:2013902/150‑1 (MQ=255)
                               cAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAt                                                                                                                <  1:4986749/150‑1 (MQ=255)
                                aaaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                               >  2:1949542/1‑150 (MQ=255)
                                 aaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtc                                                                                                              <  2:1486026/150‑1 (MQ=255)
                                 aaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATtc                                                                                                              >  1:5095730/1‑150 (MQ=255)
                                    ctcGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTcc                                                                                                           <  1:984716/150‑1 (MQ=255)
                                        gTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                       <  2:168988/150‑1 (MQ=255)
                                        gTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                       >  2:827880/1‑150 (MQ=255)
                                         tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTg                                                                                                      >  2:2822557/1‑150 (MQ=255)
                                         tAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTg                                                                                                      <  2:910477/150‑1 (MQ=255)
                                             tGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGt                                                                                                  <  2:1382926/150‑1 (MQ=255)
                                              gATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTa                                                                                                 <  1:1447475/150‑1 (MQ=255)
                                               aTTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAg                                                                                                >  2:4405777/1‑150 (MQ=255)
                                                     gcACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTCGGGaaaa                                                                                          >  1:4204560/1‑150 (MQ=255)
                                                      cACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAAt                                                                                         >  2:303929/1‑150 (MQ=255)
                                                      cACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAAt                                                                                         <  1:262438/150‑1 (MQ=255)
                                                           tATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAACATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAt                                                                                    <  1:2280986/150‑1 (MQ=255)
                                                             ttAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCt                                                                                  <  2:4587198/150‑1 (MQ=255)
                                                               aaCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGa                                                                                <  2:2281598/150‑1 (MQ=255)
                                                                aCTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGaa                                                                               <  1:1807460/150‑1 (MQ=255)
                                                                  tATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGACCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATc                                                                             >  2:4842250/1‑150 (MQ=255)
                                                                  tATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATc                                                                             <  1:643539/150‑1 (MQ=255)
                                                                   aTTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCa                                                                            <  1:4482418/150‑1 (MQ=255)
                                                                    ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAg                                                                           <  2:275716/150‑1 (MQ=255)
                                                                    ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAg                                                                           <  1:4365087/150‑1 (MQ=255)
                                                                     tttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAgg                                                                          <  1:1829199/150‑1 (MQ=255)
                                                                        acacCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAt                                                                       >  2:3678189/1‑150 (MQ=255)
                                                                        acacCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAAt                                                                       >  1:3380393/1‑150 (MQ=255)
                                                                         cacCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGTATCTGGAAtt                                                                      >  2:2658632/1‑150 (MQ=255)
                                                                                 aTAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGa                                                              <  2:4277315/150‑1 (MQ=255)
                                                                                 aTAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCTTGAGTCTCTGTGCGCTCATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATgt                                                              >  2:5116965/1‑149 (MQ=255)
                                                                                  tAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGaa                                                             >  2:3606882/1‑150 (MQ=255)
                                                                                    aGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAAc                                                           >  1:3179434/1‑150 (MQ=255)
                                                                                    aGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTGGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAAc                                                           <  1:4665677/150‑1 (MQ=255)
                                                                                      cAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCt                                                         <  1:2961127/150‑1 (MQ=255)
                                                                                      cAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCt                                                         <  2:2064791/150‑1 (MQ=255)
                                                                                          aataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaa                                                     <  1:4374016/150‑1 (MQ=255)
                                                                                           atatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaaa                                                    >  1:1226749/1‑150 (MQ=255)
                                                                                            tatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGGATTATATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaaaa                                                   >  1:2740759/1‑150 (MQ=255)
                                                                                             atTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATACAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAac                                                  >  2:3544718/1‑150 (MQ=255)
                                                                                             atTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAac                                                  >  2:349852/1‑150 (MQ=255)
                                                                                              tttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacc                                                 <  2:3942733/150‑1 (MQ=255)
                                                                                              tttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacc                                                 <  2:4982795/150‑1 (MQ=255)
                                                                                               ttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacca                                                >  2:4078150/1‑150 (MQ=255)
                                                                                               ttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacca                                                <  2:3134136/150‑1 (MQ=255)
                                                                                                 cggcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAACAATATTTCGTGCGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACAGCTGAATCAGGAATTCACAATGAAACCTGTACAaccaca                                              >  2:78908/1‑149 (MQ=255)
                                                                                                 cggcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAaccacc                                              >  1:2819116/1‑150 (MQ=255)
                                                                                                  ggcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCt                                             <  1:3012955/150‑1 (MQ=255)
                                                                                                  ggcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCt                                             >  1:1413293/1‑150 (MQ=255)
                                                                                                  ggcggGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCt                                             <  1:3572685/150‑1 (MQ=255)
                                                                                                  ggcggGAACACACTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCt                                             <  1:4405777/150‑1 (MQ=255)
                                                                                                      ggAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTa                                         >  1:1528863/1‑150 (MQ=255)
                                                                                                       gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAt                                        <  2:4208184/150‑1 (MQ=255)
                                                                                                       gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAt                                        >  1:332145/1‑150 (MQ=255)
                                                                                                        aaCACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTATCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAtt                                       <  2:1436559/150‑1 (MQ=255)
                                                                                                             ccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATgg                                  <  1:1054592/150‑1 (MQ=255)
                                                                                                                ccccGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACg                               <  1:3555595/150‑1 (MQ=255)
                                                                                                                 cccGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGa                              >  2:3694841/1‑150 (MQ=255)
                                                                                                                   cgccgAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGt                            <  1:199899/150‑1 (MQ=255)
                                                                                                                   cgccgAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTAGTAATGGACGAGt                            >  1:3219971/1‑150 (MQ=255)
                                                                                                                    gccgAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTg                           >  2:1607114/1‑150 (MQ=255)
                                                                                                                       gAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCg                        <  2:3380908/150‑1 (MQ=255)
                                                                                                                       gAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCg                        <  1:2332768/150‑1 (MQ=255)
                                                                                                                        aaCTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCAGTAAAACCACCTCGTATTAATGGACGAGTGCCgg                       >  1:4306621/1‑150 (MQ=255)
                                                                                                                             aaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTg                  >  1:1590061/1‑150 (MQ=255)
                                                                                                                             aaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACATGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTg                  >  1:3234086/1‑150 (MQ=255)
                                                                                                                              aaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGt                 >  1:3763909/1‑150 (MQ=255)
                                                                                                                               aaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                >  1:2424401/1‑150 (MQ=255)
                                                                                                                               aaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                <  1:1098191/150‑1 (MQ=255)
                                                                                                                                aaTATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCg               <  2:4681074/150‑1 (MQ=255)
                                                                                                                                  tatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGc             >  1:3674170/1‑150 (MQ=255)
                                                                                                                                    tatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcac           >  2:2897985/1‑150 (MQ=255)
                                                                                                                                    tatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATATGAAGCAGGAATTAACAATGAAACCGGTAATACAACCTCGTATTAGTGGACGAGGGCCGGTCCTGTCGGcac           >  2:2657296/1‑150 (MQ=255)
                                                                                                                                      ttCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAg         <  2:3692285/150‑1 (MQ=255)
                                                                                                                                       tCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAgg        >  2:3859147/1‑150 (MQ=255)
                                                                                                                                        cAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGa       >  2:115152/1‑150 (MQ=255)
                                                                                                                                        cAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGa       >  2:4916333/1‑150 (MQ=255)
                                                                                                                                         aaTCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGaa      >  2:3186319/1‑150 (MQ=255)
                                                                                                                                           tCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGc    <  2:1249545/150‑1 (MQ=255)
                                                                                                                                            cGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg   <  2:561676/150‑1 (MQ=255)
                                                                                                                                             gTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCgg  >  1:4442094/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TGGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769617‑1769907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑