Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,783,091 G→T 100% E134* (GAG→TAG)  aroH → 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,783,0910GT98.6% 1031.8 / 1.6 330E134* (GAG→TAG) aroH3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (149/174);  total (152/178)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 330 total aligned reads displayed.

CGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAG  >  CP009273/1782944‑1783238
                                                                                                                                                   |                                                                                                                                                   
cGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAg                                                                                                                                                   <  2:4947306/150‑1 (MQ=255)
 ggCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGc                                                                                                                                                  <  1:1758688/150‑1 (MQ=255)
   cTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTg                                                                                                                                                >  2:200579/1‑150 (MQ=255)
    tGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTgg                                                                                                                                               <  1:2789008/150‑1 (MQ=255)
     ggAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTggg                                                                                                                                              <  1:748836/150‑1 (MQ=255)
       aaaTCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTGAATTAGCTGGGCg                                                                                                                                            <  2:919312/150‑1 (MQ=255)
       aaaTCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCg                                                                                                                                            >  2:35638/1‑150 (MQ=255)
          tCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCACATTACTTTTACAGGTAAATTAGCTGGGCGTcc                                                                                                                                         >  2:4160419/1‑150 (MQ=255)
           tgTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTccc                                                                                                                                        >  2:4129367/2‑150 (MQ=255)
             tAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCaa                                                                                                                                      >  2:1226138/1‑150 (MQ=255)
             tAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCaa                                                                                                                                      >  2:4856064/1‑150 (MQ=255)
              aaTGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAAc                                                                                                                                     >  2:1283617/1‑150 (MQ=255)
               aTGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAAcc                                                                                                                                    >  1:566879/1‑150 (MQ=255)
               aTGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAAcc                                                                                                                                    <  2:2896382/150‑1 (MQ=255)
               aTGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAAcc                                                                                                                                    >  1:2819509/1‑150 (MQ=255)
                       cTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGa                                                                                                                            >  1:1857461/1‑150 (MQ=255)
                          ttttGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGtt                                                                                                                         <  2:722760/150‑1 (MQ=255)
                            ttGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTcc                                                                                                                       >  2:2714995/1‑150 (MQ=255)
                             tGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCt                                                                                                                      >  2:4693360/1‑150 (MQ=255)
                              gAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTc                                                                                                                     <  2:3769858/150‑1 (MQ=255)
                                 aaaCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGat                                                                                                                  >  1:1249227/1‑150 (MQ=255)
                                 aaaCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGat                                                                                                                  <  2:3651522/150‑1 (MQ=255)
                                   aCCACGAACTGTTGTCGGCTGGAAAGTACTAATCTCCGATCCAGATTTAAACCGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAAGTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGatat                                                                                                                <  1:3711443/150‑1 (MQ=255)
                                   aCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGatat                                                                                                                <  2:2634430/150‑1 (MQ=255)
                                    ccACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATg                                                                                                               >  2:2178440/1‑150 (MQ=255)
                                        gAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGa                                                                                                           <  2:100746/150‑1 (MQ=255)
                                          aCTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCATGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGAcc                                                                                                         <  1:69860/150‑1 (MQ=255)
                                          aCTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGAcc                                                                                                         <  2:491889/150‑1 (MQ=255)
                                              ttgtCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGta                                                                                                     <  2:4483454/150‑2 (MQ=255)
                                               tgtCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCa                                                                                                    >  2:618931/1‑150 (MQ=255)
                                                      tGGAAAGGACTAATCTCTGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGGTCCTCGATATGGTGACCGGTCAGTTTAtt                                                                                             >  1:2587304/1‑150 (MQ=255)
                                                      tGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTAtt                                                                                             <  1:1568056/150‑1 (MQ=255)
                                                       ggAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTg                                                                                            <  1:4442756/150‑1 (MQ=255)
                                                           aGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATTGTGACCGGTCAGTTTATTGCTGa                                                                                        <  2:1185392/150‑1 (MQ=255)
                                                            ggACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAt                                                                                       <  2:1584679/150‑1 (MQ=255)
                                                              aCTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAttt                                                                                     >  2:2521754/1‑150 (MQ=255)
                                                                     tcCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGt                                                                              >  1:4134959/1‑150 (MQ=255)
                                                                       cGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTg                                                                            >  1:1287680/1‑150 (MQ=255)
                                                                        gATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTgg                                                                           >  1:1413806/1‑150 (MQ=255)
                                                                         aTCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTggg                                                                          >  1:4689575/1‑150 (MQ=255)
                                                                         aTCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTggg                                                                          <  2:75506/150‑1 (MQ=255)
                                                                         aTCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTGATTGCTGATTTAATCAGTTggg                                                                          >  1:1360944/1‑150 (MQ=255)
                                                                          tCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTCAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTgggg                                                                         >  2:1888573/1‑150 (MQ=255)
                                                                          tCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTgggg                                                                         <  2:1189133/150‑1 (MQ=255)
                                                                             aGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGgcgc                                                                      >  2:5098127/1‑150 (MQ=255)
                                                                              gATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGcgcg                                                                     <  1:2212837/150‑1 (MQ=255)
                                                                               aTTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGa                                                                    <  1:3298850/150‑1 (MQ=255)
                                                                                  tAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTg                                                                 >  2:143329/1‑150 (MQ=255)
                                                                                  tAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTg                                                                 >  2:4202604/1‑150 (MQ=255)
                                                                                   aaaCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCTTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTgg                                                                <  2:2385296/150‑1 (MQ=255)
                                                                                   aaaCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGGAAATTAGCTGGGCGTCCCCACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTGTATTGCTGAGTTAATCAGGTGGGGCGCGAGTgg                                                                >  1:4371564/1‑150 (MQ=255)
                                                                                    aaCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGgc                                                               >  2:3441165/1‑150 (MQ=255)
                                                                                    aaCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGgc                                                               >  2:2615214/1‑150 (MQ=255)
                                                                                    aaCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGgc                                                               <  2:3962076/150‑1 (MQ=255)
                                                                                    aaCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGgc                                                               <  2:1605083/150‑1 (MQ=255)
                                                                                        gCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCAc                                                           <  2:4584194/150‑1 (MQ=255)
                                                                                           gCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGta                                                        <  1:1162647/150‑1 (MQ=255)
                                                                                            cTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtac                                                       >  1:2388272/1‑150 (MQ=255)
                                                                                              aTCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtacta                                                     >  1:3149639/1‑150 (MQ=255)
                                                                                              aTCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtacta                                                     <  1:3656243/150‑1 (MQ=255)
                                                                                              aTCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtacta                                                     >  2:1697057/1‑150 (MQ=255)
                                                                                              aTCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtacta                                                     >  2:1574195/1‑150 (MQ=255)
                                                                                                 gggTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCg                                                  <  2:1680548/150‑1 (MQ=255)
                                                                                                 gggTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCg                                                  >  2:868103/1‑150 (MQ=255)
                                                                                                 gggTAAATCACGGTCTGGAGCTGGCGCGCAAAGTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCg                                                  <  2:1465512/150‑1 (MQ=255)
                                                                                                   gTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGaa                                                <  2:1121782/150‑1 (MQ=255)
                                                                                                     aaaTCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATCTGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAg                                              >  1:4209429/1‑150 (MQ=255)
                                                                                                      aaTCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGt                                             >  2:374344/1‑150 (MQ=255)
                                                                                                       aTCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTc                                            <  1:605312/150‑1 (MQ=255)
                                                                                                         cACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAg                                          >  2:322449/1‑150 (MQ=255)
                                                                                                           cGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGAt                                        <  2:229077/150‑1 (MQ=255)
                                                                                                           cGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAGTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGAt                                        <  2:314695/150‑1 (MQ=255)
                                                                                                           cGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGAGATGGTGACCGGTCAGTGTAGTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGAt                                        >  1:4568024/1‑150 (MQ=255)
                                                                                                            ggTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATc                                       <  1:1074474/150‑1 (MQ=255)
                                                                                                            ggTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATc                                       <  1:194654/150‑1 (MQ=255)
                                                                                                            ggTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATc                                       >  2:1483001/1‑150 (MQ=255)
                                                                                                             gTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATcc                                      <  2:282907/150‑1 (MQ=255)
                                                                                                              tCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCa                                     >  1:2885593/1‑150 (MQ=255)
                                                                                                              tCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCa                                     <  1:897899/150‑1 (MQ=255)
                                                                                                               cTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATCTGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCAc                                    >  1:4765349/1‑150 (MQ=255)
                                                                                                               cTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCAc                                    <  1:5039625/150‑1 (MQ=255)
                                                                                                                   aGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACcgcg                                <  2:3018075/150‑1 (MQ=255)
                                                                                                                   aGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACcgcg                                <  1:2519565/150‑1 (MQ=255)
                                                                                                                       ggCGCGCAAATTACTTTTACAGGTATATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGatat                            <  1:1270488/150‑1 (MQ=255)
                                                                                                                       ggCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAAt                            <  1:4856064/150‑1 (MQ=255)
                                                                                                                       ggCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAAt                            <  1:4930921/150‑1 (MQ=255)
                                                                                                                         cgcgcAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATgg                          >  1:1796277/1‑150 (MQ=255)
                                                                                                                         cgcgcAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATgg                          >  1:3010574/1‑150 (MQ=255)
                                                                                                                          gcgcAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGc                         <  2:3325599/150‑1 (MQ=255)
                                                                                                                           cgcAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCt                        >  2:4396821/1‑150 (MQ=255)
                                                                                                                             cAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTc                      <  2:3070004/150‑1 (MQ=255)
                                                                                                                             cAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTc                      <  2:1347827/150‑1 (MQ=255)
                                                                                                                                 ttACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCa                  >  2:1653470/1‑150 (MQ=255)
                                                                                                                                 ttACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGAGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCa                  >  2:208098/1‑150 (MQ=255)
                                                                                                                                   aCTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCAct                <  1:32227/150‑1 (MQ=255)
                                                                                                                                   aCTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCAct                >  1:4534646/1‑150 (MQ=255)
                                                                                                                                    cTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCActc               <  1:200579/150‑1 (MQ=255)
                                                                                                                                      tttACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACtctc             >  2:2059470/1‑150 (MQ=255)
                                                                                                                                       ttACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTcc            >  2:1508617/1‑150 (MQ=255)
                                                                                                                                        tACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGACTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCt           <  1:3872497/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTAG  >  CP009273/1782944‑1783238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑