BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,784,871	C→A	36.6%	D211Y (GAT→TAT)	ydiU ←	UPF0061 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,784,871	0	C	A	36.6%	373.9 / 275.2	287	D211Y (GAT→TAT)	ydiU	UPF0061 family protein
Reads supporting (aligned to +/- strand): ref base C (95/87); new base A (63/42); total (158/129)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 308 total aligned reads displayed.

AAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCC  >  CP009273/1784722‑1785019

 aaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatca                                                                                                                                                     >  1:2928144/1‑150 (MQ=255)

  aCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGGCAACATCGCTAAACCAGAGACGGTATTTGTCCTcataat                                                                                                                                                    >  1:773792/1‑150 (MQ=255)

   nggCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCatcatc                                                                                                                                                   >  1:3028945/2‑150 (MQ=255)

         gTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAg                                                                                                                                             >  2:1491200/1‑150 (MQ=255)

          tAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGa                                                                                                                                            >  1:4731438/1‑150 (MQ=255)

           aaTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAt                                                                                                                                           >  2:4469372/1‑150 (MQ=255)

              cAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAtgtg                                                                                                                                        <  1:2695771/150‑1 (MQ=255)

                aGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGAc                                                                                                                                      >  2:1002624/1‑150 (MQ=255)

                      aGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTaa                                                                                                                                <  1:673133/150‑1 (MQ=255)

                         cccAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGa                                                                                                                             <  2:2916994/150‑1 (MQ=255)

                               agcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATa                                                                                                                       >  2:1829163/1‑150 (MQ=255)

                                  gACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCa                                                                                                                    <  2:3603252/150‑1 (MQ=255)

                                   aCATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCaa                                                                                                                   >  2:4723116/1‑150 (MQ=255)

                                      tgttgtCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGt                                                                                                                >  2:743948/1‑150 (MQ=255)

                                       gttgtCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTc                                                                                                               <  2:3823204/150‑1 (MQ=255)

                                         tgtCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTCATGACGAATAGCAAAGTCAg                                                                                                             >  2:4563613/1‑150 (MQ=255)

                                          gtCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGc                                                                                                            <  2:794643/150‑1 (MQ=255)

                                           tCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCt                                                                                                           <  1:640444/150‑1 (MQ=255)

                                            cGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTa                                                                                                          <  2:2550163/150‑1 (MQ=255)

                                             ggTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTaa                                                                                                         <  2:3286324/150‑1 (MQ=255)

                                                aTTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTg                                                                                                      <  2:1744822/150‑1 (MQ=255)

                                                  tcatcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGAc                                                                                                    <  2:1666314/150‑1 (MQ=255)

                                                    atcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATACTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGa                                                                                                  >  2:3872405/1‑150 (MQ=255)

                                                     tcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATTACGAATAGCAAAGTAAGCTAACTGACGaa                                                                                                 >  2:2373516/1‑150 (MQ=255)

                                                     tcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGaa                                                                                                 >  2:2856575/1‑150 (MQ=255)

                                                      caCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAc                                                                                                >  2:2316056/1‑150 (MQ=255)

                                                      caCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAc                                                                                                <  1:4327825/150‑1 (MQ=255)

                                                      caCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAc                                                                                                >  1:5038816/1‑150 (MQ=255)

                                                       aCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAcc                                                                                               >  1:3734736/1‑150 (MQ=255)

                                                       aCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAcc                                                                                               <  2:1908971/150‑1 (MQ=255)

                                                        ccccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCt                                                                                              <  2:685539/150‑1 (MQ=255)

                                                         cccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCtt                                                                                             <  2:3995371/150‑1 (MQ=255)

                                                          ccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTCACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTGGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCttt                                                                                            <  2:4802236/150‑1 (MQ=255)

                                                          ccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCttt                                                                                            >  1:102361/1‑150 (MQ=255)

                                                           cATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCtttt                                                                                           <  1:824935/150‑1 (MQ=255)

                                                           cATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCtttt                                                                                           >  2:3517240/1‑150 (MQ=255)

                                                            aTGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTc                                                                                          >  2:147196/1‑150 (MQ=255)

                                                            aTGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTc                                                                                          >  2:2450943/1‑150 (MQ=255)

                                                               aGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                                                       >  1:2035431/1‑150 (MQ=255)

                                                               aGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                                                       >  2:2824626/1‑150 (MQ=255)

                                                               aGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGa                                                                                       <  1:1121813/150‑1 (MQ=255)

                                                                  aaaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTc                                                                                    >  2:1051682/1‑150 (MQ=255)

                                                                  aaaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTc                                                                                    >  2:3602633/1‑150 (MQ=255)

                                                                  aaaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTc                                                                                    >  1:1766706/1‑150 (MQ=255)

                                                                   aaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTcg                                                                                   <  2:2689398/150‑1 (MQ=255)

                                                                   aaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTcg                                                                                   >  2:4956387/1‑150 (MQ=255)

                                                                        gACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCgcggcg                                                                              >  2:4158414/1‑150 (MQ=255)

                                                                         aCCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGcggcgg                                                                             >  1:2220969/1‑150 (MQ=255)

                                                                          ccGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgt                                                                            <  1:4843006/150‑1 (MQ=255)

                                                                            gTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgtag                                                                          <  1:1419640/150‑1 (MQ=255)

                                                                              cTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgtagta                                                                        <  1:4625125/150‑1 (MQ=255)

                                                                              cTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGgtagta                                                                        <  1:1303387/150‑1 (MQ=255)

                                                                                      gggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGtt                                                                >  1:3107252/1‑150 (MQ=255)

                                                                                      gggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGtt                                                                >  1:1598003/1‑150 (MQ=255)

                                                                                            ttAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAAt                                                          >  1:3032711/1‑150 (MQ=255)

                                                                                             tAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATg                                                         >  1:593395/1‑150 (MQ=255)

                                                                                             tAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATg                                                         >  2:2340220/1‑150 (MQ=255)

                                                                                                cgactCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATATCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGAcc                                                      <  2:3063637/150‑1 (MQ=255)

                                                                                                cgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGAcc                                                      >  1:1465452/1‑150 (MQ=255)

                                                                                                   cgcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaaa                                                   <  1:3203604/150‑1 (MQ=255)

                                                                                                   cgcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaaa                                                   >  2:53781/1‑150 (MQ=255)

                                                                                                   cgcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaaa                                                   <  2:3681275/150‑1 (MQ=255)

                                                                                                   cgcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaaa                                                   >  2:2651996/1‑150 (MQ=255)

                                                                                                    gcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGCTGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAg                                                  <  2:1290968/150‑1 (MQ=255)

                                                                                                        tACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCa                                              <  1:3804738/150‑1 (MQ=255)

                                                                                                          cGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGa                                            >  1:3368673/1‑150 (MQ=255)

                                                                                                          cGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGa                                            >  2:2259229/1‑150 (MQ=255)

                                                                                                          cGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTACTGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGa                                            >  2:2938588/1‑150 (MQ=255)

                                                                                                            tGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATg                                          <  1:421739/150‑1 (MQ=255)

                                                                                                              cGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGAc                                        >  1:4539972/1‑150 (MQ=255)

                                                                                                               gACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACt                                       >  1:880604/1‑150 (MQ=255)

                                                                                                                acaacaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACtt                                      <  2:1966169/150‑1 (MQ=255)

                                                                                                                 caacaTCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTg                                     >  2:3389332/1‑150 (MQ=255)

                                                                                                                  aacaTCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACAAAAGCGCAGATGACTTgg                                    >  2:4082097/1‑150 (MQ=255)

                                                                                                                   acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGt                                   <  1:3561326/150‑1 (MQ=255)

                                                                                                                     aTCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGc                                 >  2:4320528/1‑150 (MQ=255)

                                                                                                                       cGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCca                               >  2:3670718/1‑150 (MQ=255)

                                                                                                                         cTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGAGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCcaca                             >  2:2738218/1‑150 (MQ=255)

                                                                                                                          tAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                            >  1:2119457/1‑150 (MQ=255)

                                                                                                                          tAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                            <  1:1932081/150‑1 (MQ=255)

                                                                                                                           aaaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACg                           >  1:3073275/1‑150 (MQ=255)

                                                                                                                           aaaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACg                           <  1:4469372/150‑1 (MQ=255)

                                                                                                                                 gagaCGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAg                     <  1:865553/150‑1 (MQ=255)

                                                                                                                                  agaCGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGc                    <  1:2461506/150‑1 (MQ=255)

                                                                                                                                   gaCGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCa                   <  2:141846/150‑1 (MQ=255)

                                                                                                                                    aCGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCAt                  >  1:348161/1‑150 (MQ=255)

                                                                                                                                     cGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATc                 <  1:2829476/150‑1 (MQ=255)

                                                                                                                                     cGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATc                 <  1:2857104/150‑1 (MQ=255)

                                                                                                                                       gTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgc               >  1:3862176/1‑150 (MQ=255)

                                                                                                                                        tATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgcg              <  1:3941454/150‑1 (MQ=255)

                                                                                                                                             gTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTgg         <  1:5099536/150‑1 (MQ=255)

                                                                                                                                             gTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTgg         <  1:3602633/150‑1 (MQ=255)

                                                                                                                                              tCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGc        >  2:1206826/1‑150 (MQ=255)

                                                                                                                                               ccTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCt       >  1:1144899/1‑150 (MQ=255)

                                                                                                                                                cTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTc      >  1:2657425/1‑150 (MQ=255)

                                                                                                                                                cTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTc      >  2:3460496/1‑150 (MQ=255)

                                                                                                                                                cTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTc      <  1:4041141/150‑1 (MQ=255)

                                                                                                                                                 tcatcaTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTcc     <  1:1002310/150‑1 (MQ=255)

                                                                                                                                                  catcatCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccg    >  2:823889/1‑150 (MQ=255)

                                                                                                                                                    tcatcTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccgcc  <  2:5052701/150‑1 (MQ=255)

AAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCC  >  CP009273/1784722‑1785019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`