BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,801,745	T→G	7.9%	L30R (CTG→CGG)	ydiZ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,801,745	0	T	G	7.9%	708.9 / 46.0	262	L30R (CTG→CGG)	ydiZ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (114/126); new base G (13/8); total (128/134)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

CTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAG  >  CP009273/1801596‑1801894

cTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCt                                                                                                                                                       <  1:4912948/150‑1 (MQ=255)

 tGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTg                                                                                                                                                      <  2:236813/150‑1 (MQ=255)

 tGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTg                                                                                                                                                      <  1:2748839/150‑1 (MQ=255)

   tCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGAc                                                                                                                                                    >  1:2547928/1‑150 (MQ=255)

    cGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACt                                                                                                                                                   >  1:1009489/1‑150 (MQ=255)

    cGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACt                                                                                                                                                   <  2:3169492/150‑1 (MQ=255)

      gCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTcg                                                                                                                                                 <  1:3456085/150‑1 (MQ=255)

        aaCGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTcgcg                                                                                                                                               >  2:4702615/1‑150 (MQ=255)

                  tcCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCGGACTCGCGACGGtttttt                                                                                                                                     <  2:2982452/150‑1 (MQ=255)

                   ccGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTg                                                                                                                                    <  2:3254046/150‑1 (MQ=255)

                   ccGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTg                                                                                                                                    <  2:2064421/150‑1 (MQ=255)

                     gACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCt                                                                                                                                  <  2:3011806/150‑1 (MQ=255)

                           gCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTAt                                                                                                                            <  2:742373/150‑1 (MQ=255)

                            cTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATg                                                                                                                           <  1:4996702/150‑1 (MQ=255)

                              cAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGAc                                                                                                                         <  2:2521676/150‑1 (MQ=255)

                               aaaaGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACg                                                                                                                        <  2:2715595/150‑1 (MQ=255)

                                 aaGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGga                                                                                                                      >  1:3085350/1‑150 (MQ=255)

                                  aGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGgat                                                                                                                     >  2:1510931/1‑150 (MQ=255)

                                     cATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGgatgat                                                                                                                  >  1:1520820/1‑150 (MQ=255)

                                     cATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGgatgat                                                                                                                  <  2:1861636/150‑1 (MQ=255)

                                        gtgATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGACATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAg                                                                                                               <  2:4327592/150‑1 (MQ=255)

                                           aTAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGa                                                                                                            >  1:121953/1‑150 (MQ=255)

                                              aCAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTGCCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAat                                                                                                         <  1:3317929/150‑1 (MQ=255)

                                                aaaGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAatat                                                                                                       >  1:438864/1‑150 (MQ=255)

                                                aaaGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAatat                                                                                                       >  2:3694161/1‑150 (MQ=255)

                                                    gggTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCAt                                                                                                   >  2:1195280/1‑150 (MQ=255)

                                                       tGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAg                                                                                                >  2:1734175/1‑150 (MQ=255)

                                                        gAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGc                                                                                               <  2:1609162/150‑1 (MQ=255)

                                                         aaCTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCt                                                                                              <  1:558469/150‑1 (MQ=255)

                                                          aCTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTg                                                                                             <  1:1005482/150‑1 (MQ=255)

                                                            tATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTggg                                                                                           >  2:439885/1‑150 (MQ=255)

                                                            tATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTggg                                                                                           >  1:4660186/1‑150 (MQ=255)

                                                            tATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTggg                                                                                           >  1:2882464/1‑150 (MQ=255)

                                                             aTGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGa                                                                                          >  2:4546968/1‑150 (MQ=255)

                                                              tGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGaa                                                                                         <  2:1079194/150‑1 (MQ=255)

                                                               ggCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAAc                                                                                        <  1:310512/150‑1 (MQ=255)

                                                                 ccAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTa                                                                                      <  1:3242517/150‑1 (MQ=255)

                                                                   aGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAac                                                                                    >  1:2618496/1‑150 (MQ=255)

                                                                   aGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAAAGAGTTGACTAATTACCTGACTCGCGACGGTTTTTTGCTCACTCTGACGGATGATGAGGGAAATATCCATGAGCTGGGAACAAac                                                                                    >  1:728545/1‑150 (MQ=255)

                                                                      ggCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGAGACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACt                                                                                 <  2:3509010/150‑1 (MQ=255)

                                                                        cGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACttt                                                                               <  2:1240600/150‑1 (MQ=255)

                                                                         gATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACtttt                                                                              >  1:2113330/1‑150 (MQ=255)

                                                                         gATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACtttt                                                                              >  2:2929026/1‑150 (MQ=255)

                                                                         gATCTTGTCCGTGATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATAACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCGGGGAACTAACACtttt                                                                              >  2:3409227/1‑150 (MQ=255)

                                                                           tCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTgg                                                                            <  2:4956155/150‑1 (MQ=255)

                                                                            cTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACTTTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGa                                                                           <  2:3728357/150‑1 (MQ=255)

                                                                              tGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACt                                                                         >  1:3234661/1‑150 (MQ=255)

                                                                              tGTCCGTTAGGTCTTTACAGTCATGTTGAATGAGGATACATTGACTGAAATTATCGAGTTGAATATTTACGTGAGTCGAGACGTTTTTTTGCTGAATATGACGGATGAGTATGGTTATATTCATGAGATGGGAACTAACTGTTTTGGaat                                                                         <  2:605546/150‑3 (MQ=255)

                                                                                tCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTa                                                                       >  1:3413325/1‑150 (MQ=255)

                                                                                 ccGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTAt                                                                      >  1:1894235/1‑150 (MQ=255)

                                                                                  cGTTATGTCATAACCGTCATGTTGCATGAGGATACATGGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATc                                                                     <  1:2931072/150‑1 (MQ=255)

                                                                                   gTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCa                                                                    <  1:1325296/150‑1 (MQ=255)

                                                                                     tATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGt                                                                  >  1:1152296/1‑150 (MQ=255)

                                                                                      aTGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTa                                                                 >  2:4907927/1‑150 (MQ=255)

                                                                                      aTGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTa                                                                 <  1:224981/150‑1 (MQ=255)

                                                                                               ccGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTg                                                        <  1:2997204/150‑1 (MQ=255)

                                                                                               ccGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTg                                                        >  2:3961863/1‑150 (MQ=255)

                                                                                               ccGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTg                                                        >  1:846884/1‑150 (MQ=255)

                                                                                               ccGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTg                                                        <  1:4603723/150‑1 (MQ=255)

                                                                                                   cATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTAGCTGACTCGCGACGGTTTTTTGGTCTCTATGAGGGTTGATGAGGGATATATCCATGAGCTGGGGACTGACACTTGTGGACGTCTCTGTACGCTTAGTgtaga                                                    >  2:2963308/1‑150 (MQ=255)

                                                                                                    aTGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaa                                                   <  1:4548486/150‑1 (MQ=255)

                                                                                                     tGTTGCATGAGGATACATTGACTGCAATTAACGAGTTGAATAATTACCTGCCTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaag                                                  <  2:4410752/150‑1 (MQ=255)

                                                                                                      gTTGCATGAGTATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaaga                                                 <  1:2949434/150‑1 (MQ=255)

                                                                                                      gTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaaga                                                 >  2:4124575/1‑150 (MQ=255)

                                                                                                       ttGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTgaagaagaa                                                >  1:3521700/1‑150 (MQ=255)

                                                                                                         gCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAAt                                              <  1:3589359/150‑1 (MQ=255)

                                                                                                             gAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTaga                                          >  1:3075344/1‑150 (MQ=255)

                                                                                                             gAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTaga                                          >  2:4489190/1‑150 (MQ=255)

                                                                                                                   aCATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTg                                    >  2:1970331/1‑150 (MQ=255)

                                                                                                                   aCATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTg                                    >  1:3710293/1‑150 (MQ=255)

                                                                                                                    cATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTgg                                   <  2:2043764/150‑1 (MQ=255)

                                                                                                                     aTTGACTGAAATTAACGAGTTGAATGATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGt                                  >  2:5069181/1‑150 (MQ=255)

                                                                                                                     aTTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGt                                  >  2:1810086/1‑150 (MQ=255)

                                                                                                                      ttGACTGAAATTAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGtt                                 >  1:2864312/1‑150 (MQ=255)

                                                                                                                      gtGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGtt                                 <  2:2813411/149‑1 (MQ=255)

                                                                                                                       tGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGttt                                <  1:418588/150‑1 (MQ=255)

                                                                                                                        gACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTc                               <  2:3047186/150‑1 (MQ=255)

                                                                                                                         aCTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCg                              <  2:1403353/150‑1 (MQ=255)

                                                                                                                         aCTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATACATGAGCTGGGAACTAACACTTATGGACTTATCAGTACCCAAAGTGAACAAGAAATTAGAGAACTGGTTTCg                              >  1:424845/1‑150 (MQ=255)

                                                                                                                         aCTGAAATAAACGAGTTGAATAATAACCTGACTCGCGACGGTTTTTTGCTCACTATGACTGATGATGAGGGAAATATCCATGAGCTGGTAACTAACACTTTTGTACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCg                              >  1:239454/1‑150 (MQ=255)

                                                                                                                          cTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCgg                             >  1:2708493/1‑150 (MQ=255)

                                                                                                                          cTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCgg                             <  1:464966/150‑1 (MQ=255)

                                                                                                                           tGAAATTAACGAGTTGAATAATTACCAGACACGCGACGGTATTTTGCTCACAATGAAGGATGATCAGGGAAATATCCATGAGCTGGGAACTAACACTTTAGGACAAATCAGTACCCAAAGTGAATAATAAATTAGAGAACTGCTTTCtgg                            >  1:4429010/1‑150 (MQ=255)

                                                                                                                               aTTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCtt                        >  2:72313/1‑150 (MQ=255)

                                                                                                                               aTTAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCtt                        >  1:1101425/1‑150 (MQ=255)

                                                                                                                                ttAACGAGTTGAATAATTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTa                       >  1:1274617/1‑150 (MQ=255)

                                                                                                                                  aaCGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTAcc                     >  1:171101/1‑150 (MQ=255)

                                                                                                                                   aCGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTAccc                    >  2:78788/1‑150 (MQ=255)

                                                                                                                                    cGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCa                   <  1:5134631/150‑1 (MQ=255)

                                                                                                                                     gAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCaa                  <  2:127338/150‑1 (MQ=255)

                                                                                                                                      aGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACAGGTTTCGGGGCTTACCCaaa                 >  1:4774130/1‑150 (MQ=255)

                                                                                                                                            ataatTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAATGTGCaa           >  2:2848128/1‑150 (MQ=255)

                                                                                                                                            ataatTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCaa           >  2:3626184/1‑150 (MQ=255)

                                                                                                                                            ataatTACCGGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCaa           >  2:2585691/1‑150 (MQ=255)

                                                                                                                                              aatTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAAcc         <  1:2342064/150‑1 (MQ=255)

                                                                                                                                              aatTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAAcc         >  1:3786458/1‑150 (MQ=255)

                                                                                                                                              aatTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAAcc         >  1:1779587/1‑150 (MQ=255)

                                                                                                                                               atTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCg        <  1:4773537/150‑1 (MQ=255)

                                                                                                                                                 tACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGc      <  2:121975/150‑1 (MQ=255)

                                                                                                                                                    cTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCaaa   >  2:318922/1‑150 (MQ=255)

CTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGAAATTAACGAGTTGAATAATTACCTGACTCGCGACGGTTTTTTGCTCACTATGACGGATGATGAGGGAAATATCCATGAGCTGGGAACTAACACTTTTGGACTTATCAGTACCCAAAGTGAAGAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAG  >  CP009273/1801596‑1801894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`