Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,812,514 C→A 100% D82Y (GAT→TAT)  chbF ← phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,812,5140CA99.2% 796.0 / ‑2.2 239D82Y (GAT→TAT) chbFphospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (115/122);  total (116/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

GTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATCAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTG  >  CP009273/1812365‑1812659
                                                                                                                                                     |                                                                                                                                                 
gTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATc                                                                                                                                                   <  1:294191/150‑1 (MQ=255)
      aaTCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATc                                                                                                                                             >  1:1187552/1‑150 (MQ=255)
      aaTCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATc                                                                                                                                             >  1:940339/1‑150 (MQ=255)
        tCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCtt                                                                                                                                           >  1:3733717/1‑150 (MQ=255)
         cACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCttt                                                                                                                                          <  2:3224997/150‑1 (MQ=255)
          aCCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTc                                                                                                                                         >  2:735543/1‑150 (MQ=255)
            cGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCaa                                                                                                                                       >  2:144515/1‑150 (MQ=255)
             ggAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAAt                                                                                                                                      <  1:2684863/150‑1 (MQ=255)
             ggAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAAt                                                                                                                                      >  2:417811/1‑150 (MQ=255)
              gAATGTTACGAAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATg                                                                                                                                     <  1:7430/150‑1 (MQ=255)
              gAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATg                                                                                                                                     >  1:3737266/1‑150 (MQ=255)
               aaTGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGc                                                                                                                                    >  1:626673/1‑150 (MQ=255)
                  ggTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTc                                                                                                                                 >  1:251320/1‑150 (MQ=255)
                    tACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTcgc                                                                                                                               <  1:1730391/150‑1 (MQ=255)
                    ctcGCAGACCTCTCCACACACCGCCCGCGCCCTGGGTGCCCGGACCAAGATACCCCTCACGCCACCGAATACGTACATCCAGTTCACGCGCCGGTAAGGCGCCAACGCGCAGTTGGGTAGTAACGAAAAAAGCAACTGTCAATGCTTcgc                                                                                                                               <  2:2942443/148‑1 (MQ=255)
                       gCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCgcggc                                                                                                                            >  1:1016680/1‑150 (MQ=255)
                         aGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGa                                                                                                                          <  2:4121503/150‑1 (MQ=255)
                          gACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGAt                                                                                                                         <  2:2206873/150‑1 (MQ=255)
                           aCCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATc                                                                                                                        <  1:4985959/150‑1 (MQ=255)
                            ccTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATcc                                                                                                                       >  1:873631/1‑150 (MQ=255)
                            ccTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATcc                                                                                                                       >  1:1907169/1‑150 (MQ=255)
                             cTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCa                                                                                                                      <  2:3712720/150‑1 (MQ=255)
                              tttAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAg                                                                                                                     <  2:4156459/150‑1 (MQ=255)
                                tAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATAGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCg                                                                                                                   >  2:4812391/1‑150 (MQ=255)
                                 aaacaaacCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGt                                                                                                                  >  1:1764352/1‑150 (MQ=255)
                                  aacaaacCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGGAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGtt                                                                                                                 >  1:4935192/1‑150 (MQ=255)
                                    caaacCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGtttt                                                                                                               <  2:341209/150‑1 (MQ=255)
                                          gcccgcGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAg                                                                                                         >  1:1684071/1‑150 (MQ=255)
                                            ccgcGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCt                                                                                                       <  1:244294/150‑1 (MQ=255)
                                                gcCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATATGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCAt                                                                                                   <  1:2717328/150‑1 (MQ=255)
                                                gcCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCAt                                                                                                   <  2:2625214/150‑1 (MQ=255)
                                                 ccGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATc                                                                                                  <  1:4432740/150‑1 (MQ=255)
                                                  cGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 >  1:2217636/1‑150 (MQ=255)
                                                  cGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 >  1:4785555/1‑150 (MQ=255)
                                                  cGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 <  2:1579211/150‑1 (MQ=255)
                                                  cGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 <  1:413761/150‑1 (MQ=255)
                                                  cGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 <  2:3961965/150‑1 (MQ=255)
                                                  cGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCg                                                                                                 >  1:3194686/1‑150 (MQ=255)
                                                    ttGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCggg                                                                                               >  1:3042979/1‑150 (MQ=255)
                                                     tGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACTCGCCGGTAATTGGCCAACGAGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGa                                                                                              <  1:4410668/150‑1 (MQ=255)
                                                     tGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGa                                                                                              <  2:1701531/150‑1 (MQ=255)
                                                     tGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGa                                                                                              <  2:2843577/150‑1 (MQ=255)
                                                      ggTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGAc                                                                                             <  1:5098981/150‑1 (MQ=255)
                                                         ttCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGcc                                                                                          <  1:1922846/150‑1 (MQ=255)
                                                            cTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGc                                                                                       >  1:786923/1‑150 (MQ=255)
                                                            cTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGc                                                                                       <  1:2046170/150‑1 (MQ=255)
                                                             tGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCg                                                                                      <  2:4936430/150‑1 (MQ=255)
                                                             tGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCg                                                                                      <  2:4271202/150‑1 (MQ=255)
                                                               aCCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGtt                                                                                    >  2:962436/1‑150 (MQ=255)
                                                                ccAAGATAACCATGACTTAATGGAATACGTTCATCCTGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTa                                                                                   <  2:2964010/150‑1 (MQ=255)
                                                                ccAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTa                                                                                   <  1:4614756/150‑1 (MQ=255)
                                                                 cAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTat                                                                                  <  2:4300097/150‑1 (MQ=255)
                                                                 cAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTat                                                                                  <  2:1385466/150‑1 (MQ=255)
                                                                          cATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATcc                                                                         <  1:2597740/150‑1 (MQ=255)
                                                                            tGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGt                                                                       <  1:1032978/150‑1 (MQ=255)
                                                                             gACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGtt                                                                      <  2:2369401/150‑1 (MQ=255)
                                                                             gACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGtt                                                                      <  2:1507216/150‑1 (MQ=255)
                                                                             gACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGtt                                                                      <  1:4223176/150‑1 (MQ=255)
                                                                              aCTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTg                                                                     <  2:4457421/150‑1 (MQ=255)
                                                                               cTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTgg                                                                    <  2:4032565/150‑1 (MQ=255)
                                                                                  aaTGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCag                                                                 <  1:111735/150‑1 (MQ=255)
                                                                                  aaTGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCag                                                                 <  2:1764272/150‑1 (MQ=255)
                                                                                   aTGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCaga                                                                <  1:136651/150‑1 (MQ=255)
                                                                                     ggAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAgaga                                                              <  2:1184600/150‑1 (MQ=255)
                                                                                      gAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGAt                                                             <  2:2252595/150‑1 (MQ=255)
                                                                                       aaTACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATc                                                            >  2:3883624/1‑150 (MQ=255)
                                                                                        aTACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCg                                                           >  2:5054358/1‑150 (MQ=255)
                                                                                         tACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGa                                                          <  2:274856/150‑1 (MQ=255)
                                                                                         tACGTTCATCCAGTTCACGCGCAGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGa                                                          >  1:3464772/1‑150 (MQ=255)
                                                                                            gTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGaaaa                                                       <  1:2265014/150‑1 (MQ=255)
                                                                                               cATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAaataa                                                    >  1:957064/1‑150 (MQ=255)
                                                                                                  ccAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAatat                                                 >  1:1571537/1‑150 (MQ=255)
                                                                                                  ccAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAatat                                                 >  2:1317384/1‑150 (MQ=255)
                                                                                                    aGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATcc                                               >  2:1940680/1‑150 (MQ=255)
                                                                                                     gTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATccc                                              <  2:54353/150‑2 (MQ=255)
                                                                                                     gTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCa                                              <  1:3383203/150‑1 (MQ=255)
                                                                                                        cACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGtt                                           <  1:1021081/150‑1 (MQ=255)
                                                                                                         aCGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGttt                                          >  2:4670830/1‑150 (MQ=255)
                                                                                                               cGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGttt                                    <  2:1973511/150‑1 (MQ=255)
                                                                                                               cGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGttt                                    <  2:1869181/150‑1 (MQ=255)
                                                                                                               cGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGttt                                    >  1:5058001/1‑150 (MQ=255)
                                                                                                               cGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGttt                                    <  2:4379880/150‑1 (MQ=255)
                                                                                                                ggTAATTGGCCAACGCGCAGTTGGGTGGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTa                                   >  1:1583406/1‑150 (MQ=255)
                                                                                                                    aTTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTaccac                               <  2:2136885/150‑1 (MQ=255)
                                                                                                                    aTTGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTaccac                               <  2:4705109/150‑1 (MQ=255)
                                                                                                                     ttGGCCACCGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTaccacc                              <  1:2293771/150‑1 (MQ=255)
                                                                                                                      tGGCCACCGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCt                             <  2:3086987/150‑1 (MQ=255)
                                                                                                                      tGGCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCt                             >  2:4877966/1‑150 (MQ=255)
                                                                                                                       ggCCAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCtt                            <  1:2825988/150‑1 (MQ=255)
                                                                                                                          cAACGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGa                         >  2:107623/1‑150 (MQ=255)
                                                                                                                            aCGCGCAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACa                       <  2:4102235/150‑1 (MQ=255)
                                                                                                                               cgcAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc                    >  1:4080824/1‑150 (MQ=255)
                                                                                                                               cgcAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcc                    >  2:631538/1‑150 (MQ=255)
                                                                                                                                gcAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATcca                   >  2:3564937/1‑150 (MQ=255)
                                                                                                                                 cAGTTGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATccac                  >  2:815810/1‑150 (MQ=255)
                                                                                                                                    ttGGGTAGTAACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAg               >  1:2692478/1‑150 (MQ=255)
                                                                                                                                         tagtaACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATa          >  2:3488567/1‑150 (MQ=255)
                                                                                                                                          agtaACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATaa         >  1:2728512/1‑150 (MQ=255)
                                                                                                                                            taACGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAAtt       >  2:1103173/1‑150 (MQ=255)
                                                                                                                                             aaCGAAATAAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTc      >  1:2128486/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAGTAACGAAATCAGCATCTTTCAATGCTTCGCGGCGATCCAGCGTTTTATAAAGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTG  >  CP009273/1812365‑1812659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑