Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,829,319 C→A 100% I85I (ATC→ATA)  ydjZ → TVP38/TMEM64 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,829,3190CA99.0% 965.1 / 0.4 293I85I (ATC→ATA) ydjZTVP38/TMEM64 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (145/145);  total (147/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 293 total aligned reads displayed.

TATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCT  >  CP009273/1829172‑1829465
                                                                                                                                                   |                                                                                                                                                  
   cAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCtt                                                                                                                                               <  2:3444935/150‑1 (MQ=255)
    aaTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCttt                                                                                                                                              <  1:4418524/150‑1 (MQ=255)
     aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGGTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                             <  1:2710740/150‑1 (MQ=255)
     aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                             <  1:4056686/150‑1 (MQ=255)
     aTCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTGACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTg                                                                                                                                             <  1:5156474/150‑1 (MQ=255)
       cGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGcc                                                                                                                                           <  2:1412003/150‑1 (MQ=255)
        gCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCa                                                                                                                                          <  2:1278939/150‑1 (MQ=255)
          aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAAt                                                                                                                                        <  2:3485658/150‑1 (MQ=255)
          aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAAt                                                                                                                                        <  1:4134393/150‑1 (MQ=255)
          aGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAAt                                                                                                                                        <  1:108101/150‑1 (MQ=255)
           gcgcTGCGGCGCTAGCCGCCCTGGACCAACAGGGAATAGAACGCATCAGTCAGACCTCCGGCGCCCTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATg                                                                                                                                       <  2:5117459/150‑1 (MQ=255)
            cGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGc                                                                                                                                      <  2:251500/150‑1 (MQ=255)
              ttGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGGTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGt                                                                                                                                    >  1:2249515/1‑150 (MQ=255)
                 cggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGc                                                                                                                                 >  2:3100140/1‑150 (MQ=255)
                 cggcgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCATTATTCAGTCTTACGGCGCACTGGCGGCAGTTGACTCGTTCATGTTGATGATTTTGCAGGCCATTGCTGCACCGATACCTGCGGTTTTGATAACCTTTGCCAATGCGTCGc                                                                                                                                 >  2:1837544/1‑150 (MQ=255)
                    cgTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGt                                                                                                                              >  1:4660225/1‑150 (MQ=255)
                     gTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGtt                                                                                                                             <  2:761835/150‑1 (MQ=255)
                     gTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGtt                                                                                                                             >  1:2437981/1‑150 (MQ=255)
                        tGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGGTTTTGATAACCTTTGCCAATGCGTCGCTGTTTgg                                                                                                                          >  1:4521824/1‑150 (MQ=255)
                          ccgccgTGGACCAACAGGGTATAGAACTCTTTATTCAGTCTTACGGCGCACTGGAGGCAGTTGTCTCGTTATTGTTGATGTTTTTGCAGGCCATTTCTGCACCGCTAACTGCTTTTTTGATAACCTTTGCCAATGCGTAGCTGTTTTgct                                                                                                                        <  2:4581154/150‑2 (MQ=255)
                          ccgccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGgcg                                                                                                                        <  1:745037/150‑1 (MQ=255)
                          ccgccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGgcg                                                                                                                        <  2:2068637/150‑1 (MQ=255)
                           cgccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGgcgc                                                                                                                       >  1:1813365/1‑150 (MQ=255)
                           cgccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGgcgc                                                                                                                       >  2:4150762/1‑150 (MQ=255)
                            gccgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGcgcg                                                                                                                      >  1:3315700/1‑150 (MQ=255)
                              cgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGtt                                                                                                                    >  1:3134523/1‑150 (MQ=255)
                              cgTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGtt                                                                                                                    >  1:2901984/1‑150 (MQ=255)
                               gTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTc                                                                                                                   <  1:869722/150‑1 (MQ=255)
                                tGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCt                                                                                                                  >  1:4181771/1‑150 (MQ=255)
                                tGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCt                                                                                                                  <  2:221538/150‑1 (MQ=255)
                                  gACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTgg                                                                                                                <  2:2532573/150‑1 (MQ=255)
                                   aCCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTggg                                                                                                               >  2:4788730/1‑150 (MQ=255)
                                   aCCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTggg                                                                                                               >  1:931310/1‑150 (MQ=255)
                                   aCCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTggg                                                                                                               <  2:4482452/150‑1 (MQ=255)
                                    ccAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGGTCTgggg                                                                                                              >  2:2769396/1‑150 (MQ=255)
                                       aCAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcgg                                                                                                           >  1:2008978/1‑150 (MQ=255)
                                        cAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGggcggc                                                                                                          <  1:1407037/150‑1 (MQ=255)
                                         aGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCt                                                                                                         >  1:661104/1‑150 (MQ=255)
                                         aGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCt                                                                                                         >  2:3835524/1‑150 (MQ=255)
                                          gggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                        <  1:3110854/150‑1 (MQ=255)
                                          gggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                        <  1:584729/150‑1 (MQ=255)
                                          gggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                        <  1:3408218/150‑1 (MQ=255)
                                          gggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCtt                                                                                                        >  2:3197965/1‑150 (MQ=255)
                                           ggTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTg                                                                                                       <  2:4955217/150‑1 (MQ=255)
                                              ataGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTg                                                                                                    >  1:2829925/1‑150 (MQ=255)
                                               taGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCTAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGt                                                                                                   >  2:1517146/1‑150 (MQ=255)
                                                aGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTc                                                                                                  >  2:3287325/1‑150 (MQ=255)
                                                aGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTc                                                                                                  >  1:3613217/1‑150 (MQ=255)
                                                   aCGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTg                                                                                               >  2:677576/1‑150 (MQ=255)
                                                   aCGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTg                                                                                               >  2:3611970/1‑150 (MQ=255)
                                                    cGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTgg                                                                                              >  1:3723641/1‑150 (MQ=255)
                                                       tttATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGAcc                                                                                           >  2:198721/1‑150 (MQ=255)
                                                          aTTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGt                                                                                        <  2:1027930/150‑1 (MQ=255)
                                                            tCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTc                                                                                      <  1:50931/150‑1 (MQ=255)
                                                             cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCg                                                                                     <  2:3975007/150‑1 (MQ=255)
                                                             cAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCg                                                                                     >  1:4930760/1‑150 (MQ=255)
                                                                tCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATg                                                                                  <  2:595296/150‑1 (MQ=255)
                                                                 cTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATgg                                                                                 <  2:3070862/150‑1 (MQ=255)
                                                                    aCGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcg                                                                              <  2:4265239/150‑1 (MQ=255)
                                                                    aCGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcg                                                                              >  2:4461568/1‑150 (MQ=255)
                                                                     cGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcgg                                                                             <  1:3515519/150‑1 (MQ=255)
                                                                      ggCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggc                                                                            <  2:500438/150‑1 (MQ=255)
                                                                      ggCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggc                                                                            >  1:2783199/1‑150 (MQ=255)
                                                                        cgcACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgc                                                                          <  1:5046001/150‑1 (MQ=255)
                                                                         gcACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcg                                                                         <  1:1852894/150‑1 (MQ=255)
                                                                         gcACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGATACCTGCGTTTTTGATAACCTTTGCCAATGCGTAGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcg                                                                         <  1:3100101/150‑1 (MQ=255)
                                                                           aCTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcggc                                                                       <  2:1642248/150‑1 (MQ=255)
                                                                           aCTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTCGCTGTCGTGGACCAGTTCGATGGCcggcgcggc                                                                       >  2:554428/1‑150 (MQ=255)
                                                                            cTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCcggcgcggcg                                                                      <  2:4733722/150‑1 (MQ=255)
                                                                             tGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggtgcggcgc                                                                     >  2:4895914/1‑150 (MQ=255)
                                                                             tGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCggcgcggcgc                                                                     >  2:2882490/1‑150 (MQ=255)
                                                                              ggcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCt                                                                    <  2:2372696/150‑1 (MQ=255)
                                                                              ggcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCt                                                                    <  1:2176444/150‑1 (MQ=255)
                                                                               gcggcAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCtg                                                                   >  1:4873346/1‑150 (MQ=255)
                                                                                   cAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCt                                                               >  2:434758/1‑150 (MQ=255)
                                                                                     gTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCttt                                                             >  1:3203927/1‑150 (MQ=255)
                                                                                      ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttt                                                            <  1:2754387/150‑1 (MQ=255)
                                                                                      ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttt                                                            >  1:3926878/1‑150 (MQ=255)
                                                                                      ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttt                                                            <  1:1615832/150‑1 (MQ=255)
                                                                                      ttGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttt                                                            <  2:3862232/150‑1 (MQ=255)
                                                                                        gTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCtttttt                                                          >  1:4674678/1‑150 (MQ=255)
                                                                                             gTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGc                                                     >  2:2450990/1‑150 (MQ=255)
                                                                                               tCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCa                                                   <  1:1036455/150‑1 (MQ=255)
                                                                                                cTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCag                                                  >  2:3541060/1‑150 (MQ=255)
                                                                                                    ttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTg                                              <  2:3926895/150‑1 (MQ=255)
                                                                                                    ttgATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTg                                              >  2:3556764/1‑150 (MQ=255)
                                                                                                              tGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCcgcg                                    >  1:1663826/1‑150 (MQ=255)
                                                                                                                cAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGaa                                  <  2:115940/150‑1 (MQ=255)
                                                                                                                 tggCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAg                                 >  1:3592349/2‑150 (MQ=255)
                                                                                                                   gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                               <  2:3738254/150‑1 (MQ=255)
                                                                                                                   gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                               >  1:3243679/1‑150 (MQ=255)
                                                                                                                   gCCATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtg                               <  1:984223/150‑1 (MQ=255)
                                                                                                                    ccATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtgg                              >  2:1930157/1‑150 (MQ=255)
                                                                                                                     cATTGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAgtggt                             >  1:2979294/1‑150 (MQ=255)
                                                                                                                       ttGCTGCACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGtggtgg                           >  1:3891308/1‑150 (MQ=255)
                                                                                                                            gcACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGaaaaa                      >  2:3158603/1‑150 (MQ=255)
                                                                                                                             cACCGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAAt                     <  2:381109/150‑1 (MQ=255)
                                                                                                                                cGCTACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTaa                  >  1:1889539/1‑150 (MQ=255)
                                                                                                                                   tACCTGCGTTTTTGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGAGATGGGCCGCGAAGTGGTGGAAAAATTAACCg               >  1:117109/1‑150 (MQ=255)
                                                                                                                                            ttttGATAACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCg      >  2:2032558/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATCAATCGCAGCGTTGCGGCGTTTGCCGCCGTGGACCAACAGGGTATAGAACGCTTTATTCAGTCTTACGGCGCACTGGCGGCAGTTGTCTCGTTCTTGTTGATGATTTTGCAGGCCATTGCTGCACCGCTACCTGCGTTTTTGATCACCTTTGCCAATGCGTCGCTGTTTGGCGCGTTCTGGGGCGGCTTGCTGTCGTGGACCAGTTCGATGGCCGGCGCGGCGCTGTGCTTTTTTATCGCCAGAGTGATGGGCCGCGAAGTGGTGGAAAAATTAACCGGCAAAACCGTGCT  >  CP009273/1829172‑1829465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑