Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 12,901 G→A 100% D247N (GAT→AAT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927312,9010GA99.7% 930.1 / ‑4.8 279D247N (GAT→AAT) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (143/135);  total (144/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 281 total aligned reads displayed.

GGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACA  >  CP009273/12754‑13046
                                                                                                                                                   |                                                                                                                                                 
ggtACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAAt                                                                                                                                                 >  2:4618489/1‑150 (MQ=255)
   aCTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAg                                                                                                                                              <  2:439920/150‑1 (MQ=255)
      ttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGc                                                                                                                                           <  1:3568457/150‑1 (MQ=255)
         gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCATCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                        <  2:5780/150‑1 (MQ=255)
           tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGa                                                                                                                                      <  1:470439/150‑1 (MQ=255)
           tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGa                                                                                                                                      <  1:3059090/150‑1 (MQ=255)
              ttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAcca                                                                                                                                   <  2:3262008/150‑2 (MQ=255)
                    tatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCaa                                                                                                                             >  2:3030053/1‑150 (MQ=255)
                     atCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAAc                                                                                                                            <  2:150334/150‑1 (MQ=255)
                       cGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCACCGa                                                                                                                          >  1:2143546/1‑150 (MQ=255)
                       cGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGa                                                                                                                          >  2:1962302/1‑150 (MQ=255)
                       cGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGACAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGa                                                                                                                          >  1:623222/1‑150 (MQ=255)
                          aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATcc                                                                                                                       <  1:2997411/150‑1 (MQ=255)
                          aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATcc                                                                                                                       <  2:4137004/150‑1 (MQ=255)
                           aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCg                                                                                                                      <  1:2440738/150‑1 (MQ=255)
                           aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCg                                                                                                                      <  2:2037316/150‑1 (MQ=255)
                           aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCg                                                                                                                      <  2:3618588/150‑1 (MQ=255)
                            tCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGc                                                                                                                     >  1:1839540/1‑150 (MQ=255)
                              gacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTg                                                                                                                   <  2:2401805/150‑1 (MQ=255)
                              gacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTg                                                                                                                   >  1:99691/1‑150 (MQ=255)
                              gacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTg                                                                                                                   <  2:2185707/150‑1 (MQ=255)
                                 gaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCa                                                                                                                >  1:4366802/1‑150 (MQ=255)
                                  aaGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCaa                                                                                                               >  2:2145394/1‑150 (MQ=255)
                                  aaGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCaa                                                                                                               >  2:62112/1‑150 (MQ=255)
                                      tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa                                                                                                           >  2:1933283/1‑150 (MQ=255)
                                      tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa                                                                                                           >  1:4005283/1‑150 (MQ=255)
                                      tGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa                                                                                                           >  1:1607064/1‑150 (MQ=255)
                                          ggCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc                                                                                                       >  1:1232467/1‑150 (MQ=255)
                                            cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCt                                                                                                     <  1:4779037/150‑1 (MQ=255)
                                            cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCt                                                                                                     <  2:3727086/150‑1 (MQ=255)
                                            cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCt                                                                                                     <  2:4417294/150‑1 (MQ=255)
                                             gAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTg                                                                                                    >  1:1369670/1‑150 (MQ=255)
                                                   aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaa                                                                                              >  1:1004978/1‑150 (MQ=255)
                                                     cTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGc                                                                                            >  2:1828822/1‑150 (MQ=255)
                                                      ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCg                                                                                           >  1:517182/1‑150 (MQ=255)
                                                       tCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCgg                                                                                          <  2:3035372/150‑1 (MQ=255)
                                                       tCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCgg                                                                                          <  1:1010970/150‑1 (MQ=255)
                                                        cGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGc                                                                                         >  1:4867207/1‑150 (MQ=255)
                                                            gTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaa                                                                                     <  1:1685323/150‑1 (MQ=255)
                                                                  gCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCg                                                                               >  2:320215/1‑150 (MQ=255)
                                                                   caaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                              >  2:4609300/1‑150 (MQ=255)
                                                                   caaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                              <  2:3047613/150‑1 (MQ=255)
                                                                     accaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaa                                                                            >  2:3916942/1‑150 (MQ=255)
                                                                     accaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaa                                                                            <  1:1067807/150‑1 (MQ=255)
                                                                     accaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaa                                                                            >  2:2797646/1‑150 (MQ=255)
                                                                     accaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaa                                                                            >  1:4230627/1‑150 (MQ=255)
                                                                      ccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaaa                                                                           <  2:2004916/150‑1 (MQ=255)
                                                                      ccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaaa                                                                           <  1:4467402/150‑1 (MQ=255)
                                                                        aacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATc                                                                         >  1:2014348/1‑150 (MQ=255)
                                                                        aacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATc                                                                         >  2:3716141/1‑150 (MQ=255)
                                                                         acGGTGATACCCACCT‑GGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGa                                                                       <  1:3766937/150‑1 (MQ=255)
                                                                          cGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGa                                                                       >  1:4175707/1‑150 (MQ=255)
                                                                             tGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACt                                                                    <  2:2986861/150‑1 (MQ=255)
                                                                              gATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTg                                                                   >  1:680842/1‑150 (MQ=255)
                                                                                tACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTc                                                                 <  2:2338955/150‑1 (MQ=255)
                                                                                 aCCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCt                                                                >  1:2837584/1‑150 (MQ=255)
                                                                                         ggggggTGAAGACTTCGACAGCAGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTca                                                        >  2:654721/1‑150 (MQ=255)
                                                                                            gggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTcagca                                                     <  2:146831/150‑1 (MQ=255)
                                                                                              gTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGa                                                   >  1:4251955/1‑150 (MQ=255)
                                                                                                gAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAcc                                                 <  1:4650337/150‑1 (MQ=255)
                                                                                                 aaGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCg                                                >  2:2504587/1‑150 (MQ=255)
                                                                                                 aaGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCg                                                <  1:2004320/150‑1 (MQ=255)
                                                                                                    tcTTCGACAGCCGTCTGATCAACTATCTGTTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGTAAATCGAACTGTCTTCCGCTCATCAGACCGACg                                             <  1:3932176/149‑1 (MQ=255)
                                                                                                    aCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACg                                             <  1:619817/150‑1 (MQ=255)
                                                                                                      ttCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGtt                                           <  2:280993/150‑1 (MQ=255)
                                                                                                      ttCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGtt                                           >  1:4825412/1‑150 (MQ=255)
                                                                                                       tCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAATCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTa                                          >  1:1919250/1‑150 (MQ=255)
                                                                                                       tCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTa                                          <  1:4442400/150‑1 (MQ=255)
                                                                                                        cGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTaa                                         >  2:1735827/1‑150 (MQ=255)
                                                                                                           cAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCt                                      >  1:336884/1‑150 (MQ=255)
                                                                                                           cAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCt                                      <  2:3757540/150‑1 (MQ=255)
                                                                                                            aGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTg                                     <  2:4236761/150‑1 (MQ=255)
                                                                                                               cGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCa                                  <  2:4795513/150‑1 (MQ=255)
                                                                                                               cGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCa                                  <  1:936288/150‑1 (MQ=255)
                                                                                                                 tCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATa                                <  2:3370784/150‑1 (MQ=255)
                                                                                                                  cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATAc                               >  2:1246829/1‑150 (MQ=255)
                                                                                                                  cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATAc                               >  2:4089921/1‑150 (MQ=255)
                                                                                                                    gATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                             <  1:1782123/150‑1 (MQ=255)
                                                                                                                      tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCa                           >  2:4217517/1‑150 (MQ=255)
                                                                                                                      tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCa                           >  2:4454105/1‑150 (MQ=255)
                                                                                                                       cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                          >  2:1422785/1‑150 (MQ=255)
                                                                                                                       cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                          <  1:3220672/150‑1 (MQ=255)
                                                                                                                        aaCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACt                         >  2:3176062/1‑150 (MQ=255)
                                                                                                                        aaCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACt                         >  2:4756617/1‑150 (MQ=255)
                                                                                                                        aaCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACt                         <  2:4556799/150‑1 (MQ=255)
                                                                                                                           tATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCa                      >  1:3073919/1‑150 (MQ=255)
                                                                                                                            aTCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGTGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAg                     >  1:310511/1‑150 (MQ=255)
                                                                                                                             tCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCAGCGCAACGATCCTCAGGCAAAGCAGCGCCTGAAAGAAGCGGCAGAAAAATCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGa                    >  1:828032/1‑150 (MQ=255)
                                                                                                                                ggTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgc                 >  1:575451/1‑150 (MQ=255)
                                                                                                                                 gTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgcg                <  2:428043/150‑1 (MQ=255)
                                                                                                                                   tGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAc              >  1:2120118/1‑150 (MQ=255)
                                                                                                                                   tGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAc              >  2:3293397/1‑150 (MQ=255)
                                                                                                                                    gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAcc             <  2:1629344/150‑1 (MQ=255)
                                                                                                                                    gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGAcc             <  2:5042215/150‑1 (MQ=255)
                                                                                                                                     aagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCg            >  2:938360/1‑150 (MQ=255)
                                                                                                                                      agaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCgg           >  1:4292576/1‑150 (MQ=255)
                                                                                                                                         aTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGTAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTcc        <  1:4618448/150‑1 (MQ=255)
                                                                                                                                            cAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAATTCGAACTGTCTTCCGCTCAGCAGACCGACGTTACCCTGCCAGACATCACTGCAGCCGCGACCGGTCCGaa     >  1:5027384/1‑150 (MQ=255)
                                                                                                                                            cAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGaa     >  2:1649997/1‑150 (MQ=255)
                                                                                                                                               gaaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAaca  <  2:2603666/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACA  >  CP009273/12754‑13046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑