Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,870,743 C→A 100% H272N (CAT→AAT)  yeaN → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,870,7430CA95.9% 830.4 / 20.8 279H272N (CAT→AAT) yeaNputative transporter
Reads supporting (aligned to +/- strand):  ref base C (5/7);  new base A (140/127);  total (145/134)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 284 total aligned reads displayed.

AACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGA  >  CP009273/1870596‑1870890
                                                                                                                                                   |                                                                                                                                                   
aaCTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAcataat                                                                                                                                                   <  2:2227228/150‑1 (MQ=255)
aaCTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTAcataat                                                                                                                                                   <  2:4361964/150‑1 (MQ=255)
  cTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCAtgt                                                                                                                                                 <  2:1035520/150‑1 (MQ=255)
   tCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAAtgtg                                                                                                                                                >  1:2319847/1‑150 (MQ=255)
     aCTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGaa                                                                                                                                              >  2:4305551/1‑150 (MQ=255)
      cTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGaaa                                                                                                                                             <  2:1604877/150‑1 (MQ=255)
       tGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAg                                                                                                                                            >  1:21789/1‑150 (MQ=255)
          tCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATc                                                                                                                                         <  2:648250/150‑1 (MQ=255)
           cTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATACTGTGAAAGATCa                                                                                                                                        <  1:3381078/150‑1 (MQ=255)
            tATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAg                                                                                                                                       <  2:1362158/150‑1 (MQ=255)
            tATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAg                                                                                                                                       <  2:848753/150‑1 (MQ=255)
            tATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAg                                                                                                                                       >  2:4875343/1‑150 (MQ=255)
              ttACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCg                                                                                                                                     >  2:2033204/1‑150 (MQ=255)
              ttACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCg                                                                                                                                     <  2:1549282/150‑1 (MQ=255)
                aCGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTg                                                                                                                                   <  2:3694799/150‑1 (MQ=255)
                       tattGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGc                                                                                                                            >  2:1578704/1‑150 (MQ=255)
                        attGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCa                                                                                                                           <  1:4111259/150‑1 (MQ=255)
                         ttGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAg                                                                                                                          <  2:3328737/150‑1 (MQ=255)
                          tggctggcTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAga                                                                                                                         <  2:645366/150‑2 (MQ=255)
                            gctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgt                                                                                                                       >  1:1775274/1‑150 (MQ=255)
                             ctggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgtt                                                                                                                      <  2:127363/150‑1 (MQ=255)
                               ggctTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCGTACATAATGTGAAAGATCAGCGTGGTATTGCAGcgttcg                                                                                                                    <  2:3626068/150‑1 (MQ=255)
                                   tCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGc                                                                                                                >  1:1174455/1‑150 (MQ=255)
                                   tCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGc                                                                                                                >  1:132039/1‑150 (MQ=255)
                                   tCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGc                                                                                                                >  1:2743781/1‑150 (MQ=255)
                                     cGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCt                                                                                                              >  1:4479661/1‑150 (MQ=255)
                                        cGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGa                                                                                                           <  1:1031973/150‑1 (MQ=255)
                                         gATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGAt                                                                                                          <  2:2385926/150‑1 (MQ=255)
                                                 tcaGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAg                                                                                                  <  1:2825252/150‑1 (MQ=255)
                                                   aGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTg                                                                                                >  2:4607292/1‑150 (MQ=255)
                                                   aGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTg                                                                                                >  2:1899827/1‑150 (MQ=255)
                                                    gTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTgg                                                                                               >  2:2833971/1‑150 (MQ=255)
                                                    gTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTgg                                                                                               <  1:2914599/150‑1 (MQ=255)
                                                      cACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgc                                                                                             >  1:3241536/1‑150 (MQ=255)
                                                      cACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgc                                                                                             <  1:5086026/150‑1 (MQ=255)
                                                       aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgcg                                                                                            <  1:1035483/150‑1 (MQ=255)
                                                       aCGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCAGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGAGTTGTCTTGCAGCGGTAGATGCCATGATGTGCGCAGTGGgcg                                                                                            >  2:5089664/1‑150 (MQ=255)
                                                        cGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGgcgc                                                                                           <  1:5016410/150‑1 (MQ=255)
                                                         ggCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGcgcg                                                                                          >  2:1457661/1‑150 (MQ=255)
                                                            tataGCGAAGCTCATGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGtt                                                                                       <  2:4820089/150‑1 (MQ=255)
                                                            tataGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGtt                                                                                       <  2:2144145/150‑1 (MQ=255)
                                                              taGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTgg                                                                                     <  1:5160047/150‑1 (MQ=255)
                                                                gCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGc                                                                                   >  1:3573294/1‑150 (MQ=255)
                                                                    aGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTg                                                                               >  1:4259979/1‑150 (MQ=255)
                                                                     gCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGc                                                                              >  1:2671945/1‑150 (MQ=255)
                                                                           gCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATg                                                                        <  2:4314560/150‑1 (MQ=255)
                                                                           gCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATg                                                                        <  2:4314494/150‑1 (MQ=255)
                                                                              ggTTCACAGCATGCTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACATTTCAAACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTACGTTGCCTTGAAGTGCGCAGTGGGAGCGTTTGGGCTCTGCTTTAAGCCa                                                                     >  2:4998576/1‑150 (MQ=255)
                                                                                 tCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgcg                                                                  >  1:4676651/1‑150 (MQ=255)
                                                                                 tCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgcg                                                                  >  1:38999/1‑150 (MQ=255)
                                                                                  cACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAgcgc                                                                 <  1:2481930/150‑1 (MQ=255)
                                                                                   aCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCa                                                                >  2:801305/1‑150 (MQ=255)
                                                                                   aCTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCa                                                                <  1:227349/150‑1 (MQ=255)
                                                                                     tGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAcg                                                              >  1:3133907/1‑150 (MQ=255)
                                                                                         tGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGAt                                                          >  1:239398/1‑150 (MQ=255)
                                                                                          ggTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATc                                                         >  2:2994246/1‑150 (MQ=255)
                                                                                           gTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCa                                                        <  2:2289418/150‑1 (MQ=255)
                                                                                              tgctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCt                                                     <  1:4405524/150‑1 (MQ=255)
                                                                                               gctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTg                                                    <  1:3142815/150‑1 (MQ=255)
                                                                                               gctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTGACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTg                                                    <  2:3100082/150‑1 (MQ=255)
                                                                                                ctgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTgg                                                   >  2:870851/1‑150 (MQ=255)
                                                                                                 tgcAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGa                                                  <  1:909070/150‑1 (MQ=255)
                                                                                                   cAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGAct                                                >  2:1087460/1‑150 (MQ=255)
                                                                                                      cTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTg                                             >  1:1907300/1‑150 (MQ=255)
                                                                                                       tAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGc                                            >  1:1620266/1‑150 (MQ=255)
                                                                                                        aGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                           >  2:709635/1‑150 (MQ=255)
                                                                                                        aGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCt                                           >  2:3439703/1‑150 (MQ=255)
                                                                                                         gCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCtt                                          <  1:4562831/150‑1 (MQ=255)
                                                                                                          ccACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCttt                                         >  2:382953/1‑150 (MQ=255)
                                                                                                                  gcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGtttt                                 <  1:4450037/150‑1 (MQ=255)
                                                                                                                  gcaCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGtttt                                 <  1:4305551/150‑1 (MQ=255)
                                                                                                                    aCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTgg                               <  1:2029891/150‑1 (MQ=255)
                                                                                                                    aCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTgg                               >  2:1177320/1‑150 (MQ=255)
                                                                                                                    aCCCGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTgg                               <  1:4193749/150‑1 (MQ=255)
                                                                                                                       cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTc                            <  2:3278384/150‑1 (MQ=255)
                                                                                                                       cGGTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTc                            >  1:1515036/1‑150 (MQ=255)
                                                                                                                        ggTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                           >  2:300986/1‑150 (MQ=255)
                                                                                                                        ggTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                           <  1:544273/150‑1 (MQ=255)
                                                                                                                        ggTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                           >  2:2599205/1‑150 (MQ=255)
                                                                                                                        ggTTTGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTcc                           <  2:3960696/150‑1 (MQ=255)
                                                                                                                          tttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCgg                         >  1:3424917/1‑150 (MQ=255)
                                                                                                                          tttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCAgg                         >  2:4403385/1‑150 (MQ=255)
                                                                                                                           ttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGgc                        >  1:2128318/1‑150 (MQ=255)
                                                                                                                           ttGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGgc                        >  2:5042809/1‑150 (MQ=255)
                                                                                                                            tGCTGATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGgcg                       <  1:3762015/150‑1 (MQ=255)
                                                                                                                               tGATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaa                    >  1:1415558/1‑150 (MQ=255)
                                                                                                                                gATCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac                   <  1:640707/150‑1 (MQ=255)
                                                                                                                                gATCCCACTGTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaac                   >  2:76207/1‑150 (MQ=255)
                                                                                                                                  tCCCACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGcaacaa                 >  2:1821302/1‑150 (MQ=255)
                                                                                                                                   cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAAt                >  2:3408370/1‑150 (MQ=255)
                                                                                                                                   cccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAAt                <  2:3162949/150‑1 (MQ=255)
                                                                                                                                    ccACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATg               >  2:2535458/1‑150 (MQ=255)
                                                                                                                                     cACTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGa              >  1:2244163/1‑150 (MQ=255)
                                                                                                                                       cTTTTCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATa            >  2:4652380/1‑150 (MQ=255)
                                                                                                                                         tttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACt          <  2:132039/150‑1 (MQ=255)
                                                                                                                                         tttCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACt          <  1:1802395/150‑1 (MQ=255)
                                                                                                                                           tCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTgg        >  1:4063125/1‑150 (MQ=255)
                                                                                                                                           tCTTACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTgg        >  2:1197971/1‑150 (MQ=255)
                                                                                                                                             ttACATAATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTgggg      >  2:4205840/1‑150 (MQ=255)
                                                                                                                                                cataatGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTg   <  1:3978514/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGTCACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCAGCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCAGCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCACGCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTGA  >  CP009273/1870596‑1870890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑