Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,872,054 A→T 100% E28V (GAG→GTG)  yeaP → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,872,0540AT97.6% 809.5 / 6.2 246E28V (GAG→GTG) yeaPdiguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (1/5);  new base T (121/119);  total (122/124)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

ATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTAT  >  CP009273/1871907‑1872200
                                                                                                                                                   |                                                                                                                                                  
atgTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGgtga                                                                                                                                                  <  2:1287696/150‑1 (MQ=255)
    tttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCt                                                                                                                                              >  1:535248/1‑150 (MQ=255)
    tttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCt                                                                                                                                              <  1:4850776/150‑1 (MQ=255)
      tATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTgg                                                                                                                                            <  2:3692296/150‑1 (MQ=255)
             ccTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCCGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGt                                                                                                                                     >  2:2797508/1‑150 (MQ=255)
             ccTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGt                                                                                                                                     >  2:4992652/1‑150 (MQ=255)
              cTGCAAAGATTAAATAATCAGCATTGACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGta                                                                                                                                    <  2:1776578/150‑2 (MQ=255)
                gCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCAc                                                                                                                                  <  1:2177465/150‑1 (MQ=255)
                  aaaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTg                                                                                                                                <  1:3997729/150‑1 (MQ=255)
                    aGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGat                                                                                                                              >  2:2767717/1‑150 (MQ=255)
                    aGATTAAATAATCAGCATTGACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTCGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGat                                                                                                                              <  1:2640457/150‑1 (MQ=255)
                           ataatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAAt                                                                                                                       >  2:4256381/1‑150 (MQ=255)
                                cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacc                                                                                                                  <  2:3446454/150‑1 (MQ=255)
                                 aGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacct                                                                                                                 >  1:1009228/1‑150 (MQ=255)
                                  gCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAaccta                                                                                                                <  1:4652937/150‑1 (MQ=255)
                                  gCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAaccta                                                                                                                <  2:930492/150‑1 (MQ=255)
                                  gCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAaccta                                                                                                                >  1:856804/1‑150 (MQ=255)
                                  gCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAaccta                                                                                                                >  1:2947175/1‑150 (MQ=255)
                                    aTTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctacc                                                                                                              >  2:181909/1‑150 (MQ=255)
                                     tttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctacct                                                                                                             <  2:1436626/150‑1 (MQ=255)
                                      ttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTg                                                                                                            >  1:1460581/1‑150 (MQ=255)
                                       tACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGa                                                                                                           <  1:4166680/150‑1 (MQ=255)
                                        aCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGAc                                                                                                          >  1:2834020/1‑150 (MQ=255)
                                           cgccgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaa                                                                                                       >  2:2593511/1‑150 (MQ=255)
                                           cgccgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaa                                                                                                       >  2:2748671/1‑150 (MQ=255)
                                               gTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTg                                                                                                   >  2:1259333/1‑150 (MQ=255)
                                                tATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                  >  1:3234598/1‑150 (MQ=255)
                                                  tCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGAt                                                                                                >  2:4567182/1‑150 (MQ=255)
                                                    cTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGt                                                                                              >  1:372950/1‑150 (MQ=255)
                                                     tGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTc                                                                                             >  2:4292762/1‑150 (MQ=255)
                                                       gAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGa                                                                                           <  1:275791/150‑1 (MQ=255)
                                                        aGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                          >  1:574601/1‑150 (MQ=255)
                                                        aGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                          <  2:1186018/150‑1 (MQ=255)
                                                        aGTTGGTCCGTGTCAGGTCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                          <  2:1186059/150‑1 (MQ=255)
                                                          ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgc                                                                                        >  2:622322/1‑150 (MQ=255)
                                                           tgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcg                                                                                       >  2:648752/1‑150 (MQ=255)
                                                            gttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgc                                                                                      >  2:908457/1‑150 (MQ=255)
                                                              tCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcgc                                                                                    >  1:3353741/1‑150 (MQ=255)
                                                                  tgtCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgc                                                                                <  2:4360303/150‑1 (MQ=255)
                                                                   gtCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgca                                                                               <  1:927679/150‑1 (MQ=255)
                                                                    tcagatcagaTTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcag                                                                              >  1:5080740/1‑150 (MQ=255)
                                                                      agatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcagca                                                                            <  1:1246273/150‑1 (MQ=255)
                                                                       gatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCat                                                                           <  1:229106/150‑1 (MQ=255)
                                                                         tcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCatat                                                                         <  1:3557227/150‑1 (MQ=255)
                                                                          cagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatt                                                                        <  1:755788/150‑1 (MQ=255)
                                                                            gatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtattat                                                                      <  2:2542574/150‑1 (MQ=255)
                                                                             atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATg                                                                     >  2:4820346/1‑150 (MQ=255)
                                                                               tATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGACTCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGtt                                                                   >  1:2971066/1‑150 (MQ=255)
                                                                               tATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGtt                                                                   <  2:3632023/150‑1 (MQ=255)
                                                                               tATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTGCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATCTTATGtt                                                                   >  2:1925808/1‑150 (MQ=255)
                                                                                aTCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGttt                                                                  <  2:4054677/150‑1 (MQ=255)
                                                                                    cccGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGccc                                                              <  2:3278906/150‑1 (MQ=255)
                                                                                    cccGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGccc                                                              <  1:2011286/150‑1 (MQ=255)
                                                                                     ccGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCg                                                             >  2:3620598/1‑150 (MQ=255)
                                                                                         gTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAc                                                         <  2:3873767/150‑1 (MQ=255)
                                                                                           ctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAg                                                       <  2:2346025/150‑1 (MQ=255)
                                                                                             cGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGcc                                                     >  1:1157516/1‑150 (MQ=255)
                                                                                             cGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGcc                                                     <  1:4461529/150‑1 (MQ=255)
                                                                                              gCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCa                                                    <  2:1998924/150‑1 (MQ=255)
                                                                                              gCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCa                                                    <  2:2522089/150‑1 (MQ=255)
                                                                                               cAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAg                                                   <  1:1442158/150‑1 (MQ=255)
                                                                                               cAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAg                                                   >  1:4508598/1‑150 (MQ=255)
                                                                                                 aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaa                                                 <  1:3786789/150‑1 (MQ=255)
                                                                                                 aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaa                                                 <  1:2635679/150‑1 (MQ=255)
                                                                                                 aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaa                                                 <  2:122214/150‑1 (MQ=255)
                                                                                                    ccTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAAt                                              >  2:2161058/1‑150 (MQ=255)
                                                                                                    ccTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAAt                                              <  1:5029736/150‑1 (MQ=255)
                                                                                                    ccTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAAt                                              <  1:2471644/150‑1 (MQ=255)
                                                                                                      ttGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGt                                            <  2:2887676/150‑1 (MQ=255)
                                                                                                       tGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTa                                           <  1:3750707/150‑1 (MQ=255)
                                                                                                       tGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTa                                           <  1:2370789/150‑1 (MQ=255)
                                                                                                           aaaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGGTGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                       >  2:3497512/1‑150 (MQ=255)
                                                                                                           aaaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                       <  2:3821891/150‑1 (MQ=255)
                                                                                                              gAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCg                                    <  2:1460581/150‑1 (MQ=255)
                                                                                                               aaCAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCgg                                   >  1:1601799/1‑150 (MQ=255)
                                                                                                                aCAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGga                                  >  1:185305/1‑150 (MQ=255)
                                                                                                                aCAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGga                                  >  1:3529578/1‑150 (MQ=255)
                                                                                                                 cAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgag                                 <  2:487530/150‑1 (MQ=255)
                                                                                                                  aGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgaga                                >  1:1572489/1‑150 (MQ=255)
                                                                                                                   gTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGaa                               >  2:3778890/1‑150 (MQ=255)
                                                                                                                   gTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGACTTCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGaa                               <  2:775174/150‑1 (MQ=255)
                                                                                                                      aCTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAttt                            <  1:4954671/150‑1 (MQ=255)
                                                                                                                        tGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTa                          >  1:4344834/1‑150 (MQ=255)
                                                                                                                          gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAcc                        <  2:1603604/150‑1 (MQ=255)
                                                                                                                          gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAcc                        <  1:1679051/150‑1 (MQ=255)
                                                                                                                          gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAcc                        <  2:494113/150‑1 (MQ=255)
                                                                                                                           aaaGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCg                       >  1:2196683/1‑150 (MQ=255)
                                                                                                                           aaaGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCg                       >  1:4924793/1‑150 (MQ=255)
                                                                                                                            aaGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGt                      >  1:4937216/1‑150 (MQ=255)
                                                                                                                               tCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGtctc                   <  2:5102630/150‑1 (MQ=255)
                                                                                                                                     ccGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGa             >  1:4164748/1‑150 (MQ=255)
                                                                                                                                      cGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAt            >  1:3095112/1‑150 (MQ=255)
                                                                                                                                       gACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt           >  2:4513138/1‑150 (MQ=255)
                                                                                                                                       gACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt           >  2:2478353/1‑150 (MQ=255)
                                                                                                                                        aCAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTa          >  2:3007547/1‑150 (MQ=255)
                                                                                                                                         cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTAc         >  2:3858527/1‑150 (MQ=255)
                                                                                                                                         cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTAc         >  2:4001539/1‑150 (MQ=255)
                                                                                                                                         cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTAc         >  2:1815913/1‑150 (MQ=255)
                                                                                                                                           gCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTc       <  2:4637702/150‑1 (MQ=255)
                                                                                                                                                tGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTAt  <  1:685199/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTAT  >  CP009273/1871907‑1872200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑