Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,955,401 C→A 79.5% S361S (TCC→TCA)  argS → arginyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,955,4010CA79.5% 555.4 / 181.4 298S361S (TCC→TCA) argSarginyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (29/32);  new base A (105/132);  total (134/164)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

CTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG  >  CP009273/1955254‑1955548
                                                                                                                                                   |                                                                                                                                                   
ctACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGt                                                                                                                                                   >  2:3185800/1‑150 (MQ=255)
 tACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTa                                                                                                                                                  >  2:2372857/1‑150 (MQ=255)
  acacCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAc                                                                                                                                                 >  2:3306215/1‑150 (MQ=255)
   caccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAcc                                                                                                                                                <  1:163937/150‑1 (MQ=255)
       accacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTg                                                                                                                                            <  1:4298994/150‑1 (MQ=255)
        ccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTgg                                                                                                                                           <  2:1294820/150‑1 (MQ=255)
         cacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGa                                                                                                                                          >  1:2733813/1‑150 (MQ=255)
         cacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGa                                                                                                                                          <  1:1484394/150‑1 (MQ=255)
            tGATATCGCCTTTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAaca                                                                                                                                       <  2:4696108/150‑1 (MQ=255)
            tGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAaca                                                                                                                                       <  1:786006/150‑1 (MQ=255)
            tGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAaca                                                                                                                                       <  2:145830/150‑1 (MQ=255)
             gATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAacac                                                                                                                                      <  2:1856653/150‑1 (MQ=255)
             gATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAacac                                                                                                                                      >  1:4432202/1‑150 (MQ=255)
                atCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAAcaccac                                                                                                                                   >  2:2216462/1‑150 (MQ=255)
                  cGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACAt                                                                                                                                 >  1:556824/1‑150 (MQ=255)
                     cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGtt                                                                                                                              >  2:4049958/1‑150 (MQ=255)
                           gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCat                                                                                                                        >  2:955021/1‑150 (MQ=255)
                           gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCat                                                                                                                        >  1:4707871/1‑150 (MQ=255)
                               tatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                    >  2:5131623/1‑150 (MQ=255)
                               tatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                    <  2:2675372/150‑1 (MQ=255)
                                atCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGc                                                                                                                   <  2:2424430/150‑1 (MQ=255)
                                  cGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTg                                                                                                                 <  1:4331461/150‑1 (MQ=255)
                                   gTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTgg                                                                                                                <  2:4976914/150‑1 (MQ=255)
                                     tATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGt                                                                                                              <  2:1652100/150‑1 (MQ=255)
                                     tATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGt                                                                                                              <  2:531698/150‑1 (MQ=255)
                                       tGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaa                                                                                                            <  2:3685027/150‑1 (MQ=255)
                                       tGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaa                                                                                                            <  2:3769295/150‑1 (MQ=255)
                                         aaaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCCTGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGACCACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                          >  1:5150379/1‑150 (MQ=255)
                                         aaaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                          <  1:1346735/150‑1 (MQ=255)
                                          aaCACTGCCAGCCGATCGCTTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGa                                                                                                         <  2:2642700/150‑1 (MQ=255)
                                            cacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACg                                                                                                       <  1:2156207/150‑1 (MQ=255)
                                                 cATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCaaa                                                                                                  >  2:1627333/1‑150 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                >  1:1076924/1‑150 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                <  1:187377/150‑1 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                <  2:299443/150‑1 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                <  2:2255528/150‑1 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                >  1:3657354/1‑150 (MQ=255)
                                                    gCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcca                                                                                               <  1:4917409/150‑2 (MQ=255)
                                                       gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTc                                                                                            >  1:3965460/1‑150 (MQ=255)
                                                       gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTc                                                                                            <  1:3568749/150‑1 (MQ=255)
                                                        aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGACTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAATGACGGCCAACCGTTCa                                                                                           >  1:3374434/1‑150 (MQ=255)
                                                        aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCa                                                                                           <  2:2821703/150‑1 (MQ=255)
                                                          cgcgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCaaa                                                                                         >  2:570510/1‑150 (MQ=255)
                                                           gcgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCaaaa                                                                                        >  2:3984680/1‑150 (MQ=255)
                                                            cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAAc                                                                                       <  2:4363004/150‑1 (MQ=255)
                                                             gTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGGTCAAAAcc                                                                                      >  2:4746659/1‑150 (MQ=255)
                                                               gCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCcg                                                                                    <  2:4174932/150‑1 (MQ=255)
                                                                cTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGTAATCAGTACAGCTGGAACACCACATGTTCGGCATGATTCTGGGTAAAGACGGCAAACCGTTCAAAACCcgc                                                                                   <  2:1842244/150‑1 (MQ=255)
                                                                  gTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCcgcgc                                                                                 >  1:3357227/1‑150 (MQ=255)
                                                                   tATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCACACCGTTCAAAACCcgcgcg                                                                                >  2:4459048/1‑150 (MQ=255)
                                                                     ttACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCggg                                                                              >  1:3069680/1‑150 (MQ=255)
                                                                        cATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGggtgg                                                                           >  2:4297613/1‑150 (MQ=255)
                                                                        cATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGggtgg                                                                           <  2:792812/150‑1 (MQ=255)
                                                                         aTCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGggtggt                                                                          <  2:151371/150‑1 (MQ=255)
                                                                            gACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACa                                                                       <  1:2326975/150‑1 (MQ=255)
                                                                            gACTCCCGTCAGCATCAACACCTGATGCAGGCAGGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACa                                                                       <  2:179973/150‑1 (MQ=255)
                                                                              cTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGt                                                                     <  1:2811432/150‑1 (MQ=255)
                                                                               tCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTg                                                                    >  2:3449302/1‑150 (MQ=255)
                                                                                cccGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGa                                                                   >  1:3748881/1‑150 (MQ=255)
                                                                                accGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGa                                                                   >  1:2764902/2‑150 (MQ=255)
                                                                                  cGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGaaa                                                                 >  2:2715438/1‑150 (MQ=255)
                                                                                    tCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACt                                                               <  2:2110444/150‑1 (MQ=255)
                                                                                       gCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGc                                                            >  2:4875861/1‑150 (MQ=255)
                                                                                             acacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATct                                                      <  2:3499929/150‑1 (MQ=255)
                                                                                              cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctg                                                     <  2:1914378/150‑1 (MQ=255)
                                                                                              cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctg                                                     <  1:4484916/150‑1 (MQ=255)
                                                                                                cccgATGCCCGCAGGGGCGCTCGTCCGGACCGCAGGCTCTGTCCCGGCATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCACACCGTTCAAACCCCGCGCGGGTGGTACAGTGCAACGGGCCGATctgct                                                   <  2:5085416/147‑1 (MQ=255)
                                                                                                 cTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgctg                                                  >  1:3477874/1‑150 (MQ=255)
                                                                                                  tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTgg                                                 <  1:171613/150‑1 (MQ=255)
                                                                                                  tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTgg                                                 >  2:4087505/1‑150 (MQ=255)
                                                                                                    aTGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGAt                                               >  1:4611303/1‑150 (MQ=255)
                                                                                                       cAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGaa                                            <  2:4549708/150‑1 (MQ=255)
                                                                                                          gCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGcc                                         >  1:2921563/1‑150 (MQ=255)
                                                                                                            aTGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCAGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAACCCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGATGCCCt                                       <  2:7593/150‑1 (MQ=255)
                                                                                                             tGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTg                                      <  2:1987588/150‑1 (MQ=255)
                                                                                                               ggCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGa                                    >  1:1189985/1‑150 (MQ=255)
                                                                                                                gCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGaa                                   <  1:1530860/150‑1 (MQ=255)
                                                                                                                 ctaTCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCAAGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAc                                  >  1:5078511/3‑150 (MQ=255)
                                                                                                                 cGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAc                                  >  1:2524088/1‑150 (MQ=255)
                                                                                                                   aTCGTCCGTAAAGCAGGCTATGAACCGGACTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACACGCGCGGGAGGTACAGTGACACTGGCCGATCTGCTGGATGACGCCCTGGAACGt                                >  2:1779103/1‑150 (MQ=255)
                                                                                                                     cGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGc                              >  1:730650/1‑150 (MQ=255)
                                                                                                                         cGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGc                          <  1:1970884/150‑1 (MQ=255)
                                                                                                                            aaaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                       >  1:4111805/1‑150 (MQ=255)
                                                                                                                            aaaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                       <  2:3257548/150‑1 (MQ=255)
                                                                                                                             aaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                      <  2:2336892/150‑1 (MQ=255)
                                                                                                                             aaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                      <  1:1052270/150‑1 (MQ=255)
                                                                                                                             aaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                      <  2:1256708/150‑1 (MQ=255)
                                                                                                                              aGCTGGCTATGTACCGGACTCCGTACCGCTGGAACACCACAGGTTCGTCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCt                     <  2:876271/150‑1 (MQ=255)
                                                                                                                              aGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCt                     <  1:4814931/150‑1 (MQ=255)
                                                                                                                              aGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCt                     <  2:4262193/150‑1 (MQ=255)
                                                                                                                               gCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtg                    <  1:1593984/150‑1 (MQ=255)
                                                                                                                                   gCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtggtgg                <  1:1222310/150‑1 (MQ=255)
                                                                                                                                     tATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCa              <  1:3917081/150‑1 (MQ=255)
                                                                                                                                      aTGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAg             >  2:4246547/1‑150 (MQ=255)
                                                                                                                                        gTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGACCGTGCACGCCGTCTGGTGGCAGaa           >  1:3201743/1‑150 (MQ=255)
                                                                                                                                              gAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAATACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAc     >  1:4955724/1‑150 (MQ=255)
                                                                                                                                               aaTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAcc    >  2:4608344/1‑150 (MQ=255)
                                                                                                                                                aTCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAccc   <  2:1060880/150‑1 (MQ=255)
                                                                                                                                                aTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAccc   <  1:2684831/150‑1 (MQ=255)
                                                                                                                                                aTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAccc   <  2:938930/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG  >  CP009273/1955254‑1955548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑