Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,984,987 C→A 100% T38T (ACG→ACT)  yecA ← UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,984,9870CA99.3% 999.1 / ‑2.7 299T38T (ACG→ACT) yecAUPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (131/166);  total (132/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 300 total aligned reads displayed.

TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGC  >  CP009273/1984840‑1985132
                                                                                                                                                   |                                                                                                                                                 
   ggTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCaa                                                                                                                                              >  2:4738947/1‑150 (MQ=255)
   ggTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCaa                                                                                                                                              >  2:4025357/1‑150 (MQ=255)
       tCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATaaa                                                                                                                                          >  1:556991/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAcc                                                                                                                                        >  2:2954874/1‑150 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAcc                                                                                                                                        <  1:1008855/150‑1 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAcc                                                                                                                                        <  2:1339030/150‑1 (MQ=255)
         ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAAcc                                                                                                                                        >  2:1759954/1‑150 (MQ=255)
            cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                     <  1:1120056/150‑1 (MQ=255)
            cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                     <  1:1120124/150‑1 (MQ=255)
            cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                     >  2:115935/1‑150 (MQ=255)
              tatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCa                                                                                                                                   <  1:2106392/150‑1 (MQ=255)
                 gTTGAAAAGCCAGATTCATAAAGCGCGTCATCACTTTCTCTGACGCACAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCAACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCt                                                                                                                                >  1:3490279/1‑150 (MQ=255)
                   tGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTcc                                                                                                                              <  1:4408105/150‑1 (MQ=255)
                      aaaGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGcc                                                                                                                           <  2:979133/150‑1 (MQ=255)
                      aaaGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGcc                                                                                                                           <  1:4530287/150‑1 (MQ=255)
                         gCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCcaca                                                                                                                        <  1:3401656/150‑1 (MQ=255)
                            aGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCa                                                                                                                     >  2:1820439/1‑150 (MQ=255)
                             gATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCaa                                                                                                                    >  1:4684536/1‑150 (MQ=255)
                              aTTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAg                                                                                                                   <  1:1879935/150‑1 (MQ=255)
                                   tAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATgg                                                                                                              <  1:2275884/150‑1 (MQ=255)
                                    aaaGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCTAGGATGGc                                                                                                             >  1:1710620/1‑150 (MQ=255)
                                    aaaGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGc                                                                                                             <  2:1967992/150‑1 (MQ=255)
                                      aGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGt                                                                                                           <  1:3290419/150‑1 (MQ=255)
                                        cgcgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTgg                                                                                                         >  1:2298885/1‑150 (MQ=255)
                                        cgcgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTgg                                                                                                         >  1:2298656/1‑150 (MQ=255)
                                         gcgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGt                                                                                                        >  2:5091450/1‑150 (MQ=255)
                                          cgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTc                                                                                                       <  2:2124314/150‑1 (MQ=255)
                                          cgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTc                                                                                                       >  1:884573/1‑150 (MQ=255)
                                           gTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACCCGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCa                                                                                                      <  2:2023071/150‑1 (MQ=255)
                                            tCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAg                                                                                                     <  1:3200348/150‑1 (MQ=255)
                                            tCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAg                                                                                                     >  2:193822/1‑150 (MQ=255)
                                             cATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgt                                                                                                    >  1:2254012/1‑150 (MQ=255)
                                             cATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgt                                                                                                    >  2:2318609/1‑150 (MQ=255)
                                              aTCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgtg                                                                                                   >  2:1082801/1‑150 (MQ=255)
                                                  ctTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGtgttgt                                                                                               <  1:1390570/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                              <  2:2515623/150‑1 (MQ=255)
                                                   tttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTa                                                                                              <  1:3100377/150‑1 (MQ=255)
                                                    ttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAt                                                                                             <  1:4388187/150‑1 (MQ=255)
                                                    ttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAt                                                                                             <  2:2348532/150‑1 (MQ=255)
                                                    ttCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAt                                                                                             <  2:3849181/150‑1 (MQ=255)
                                                        ctGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTgg                                                                                         <  2:4266155/150‑1 (MQ=255)
                                                         tGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGt                                                                                        >  1:5144004/1‑150 (MQ=255)
                                                            cGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTGTTTGGTCAg                                                                                     <  1:4645731/150‑1 (MQ=255)
                                                            cGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAg                                                                                     >  2:3265760/1‑150 (MQ=255)
                                                             gCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGa                                                                                    >  2:1479300/1‑150 (MQ=255)
                                                                cAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAata                                                                                 <  2:4267921/150‑1 (MQ=255)
                                                                   cgcgGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCg                                                                              >  2:1915165/1‑150 (MQ=255)
                                                                     cgGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTc                                                                            <  2:468378/150‑1 (MQ=255)
                                                                     cgGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTc                                                                            >  1:3761478/1‑150 (MQ=255)
                                                                      ggCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTCTTTGGTCAGAATATCGTcc                                                                           >  2:1891739/1‑150 (MQ=255)
                                                                       gCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCa                                                                          <  2:5047267/150‑1 (MQ=255)
                                                                       gCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCa                                                                          <  1:4093865/150‑1 (MQ=255)
                                                                        cacaTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAg                                                                         >  1:3629782/1‑150 (MQ=255)
                                                                         acaTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGc                                                                        >  2:3320751/1‑150 (MQ=255)
                                                                           aTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCa                                                                      >  2:837060/1‑150 (MQ=255)
                                                                            tAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAt                                                                     <  1:1032209/150‑1 (MQ=255)
                                                                                cAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCACGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCa                                                                 <  2:453986/150‑1 (MQ=255)
                                                                                cAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCa                                                                 >  1:5011963/1‑150 (MQ=255)
                                                                                cAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCAGTCCa                                                                 <  2:4461290/150‑1 (MQ=255)
                                                                                 aGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCaa                                                                >  2:1664347/1‑150 (MQ=255)
                                                                                  gCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAc                                                               <  1:1913074/150‑1 (MQ=255)
                                                                                  gCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAAc                                                               >  2:218721/1‑150 (MQ=255)
                                                                                   ccccaccccaCACGGCAACCAGCCACGGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACt                                                              <  2:2282484/150‑1 (MQ=255)
                                                                                      caccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCAc                                                           >  1:3842929/1‑150 (MQ=255)
                                                                                        ccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACtt                                                         >  1:673119/1‑150 (MQ=255)
                                                                                        ccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACtt                                                         >  2:2460127/1‑150 (MQ=255)
                                                                                           cacaCGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCCTTCCAACTCACTTTCg                                                      >  2:949025/1‑150 (MQ=255)
                                                                                             cacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGtt                                                    <  1:2659872/150‑1 (MQ=255)
                                                                                             cacGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGtt                                                    >  2:3195825/1‑150 (MQ=255)
                                                                                               cGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTa                                                  >  2:564607/1‑150 (MQ=255)
                                                                                                ggCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTaa                                                 <  1:2934664/150‑1 (MQ=255)
                                                                                                   aaCCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACgg                                              <  2:341099/150‑1 (MQ=255)
                                                                                                   aaCCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACgg                                              >  2:711499/1‑150 (MQ=255)
                                                                                                    aCCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTACCGGt                                             >  2:2650109/1‑150 (MQ=255)
                                                                                                    aCCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGt                                             <  1:4237266/150‑1 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                          >  2:2803142/1‑150 (MQ=255)
                                                                                                       aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                          >  2:3709735/1‑150 (MQ=255)
                                                                                                          cACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGtt                                       >  2:648696/1‑150 (MQ=255)
                                                                                                             tGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTc                                    <  1:4011130/150‑1 (MQ=255)
                                                                                                             tGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTc                                    <  1:2516665/150‑1 (MQ=255)
                                                                                                                    gTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCt                             <  1:4444034/150‑1 (MQ=255)
                                                                                                                      tCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCttt                           >  1:2542800/1‑150 (MQ=255)
                                                                                                                          tctcTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                       >  1:2632361/1‑150 (MQ=255)
                                                                                                                             ctTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgt                    >  1:1856204/1‑150 (MQ=255)
                                                                                                                              ttGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgtg                   <  1:1605707/150‑1 (MQ=255)
                                                                                                                               tGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTgg                  <  1:1790835/150‑1 (MQ=255)
                                                                                                                               tGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGAATTTGGTCAGAATATCGTCAAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTgg                  >  1:735256/1‑150 (MQ=255)
                                                                                                                                    agaACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGAtctc             >  2:3171934/1‑150 (MQ=255)
                                                                                                                                     gaACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCa            >  1:4709831/1‑150 (MQ=255)
                                                                                                                                     gaACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCa            >  1:1951614/1‑150 (MQ=255)
                                                                                                                                         tCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTc        <  2:15851/150‑1 (MQ=255)
                                                                                                                                         tCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGGTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCCCTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTc        >  2:4988101/1‑150 (MQ=255)
                                                                                                                                          cAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTAACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCg       <  2:359781/150‑1 (MQ=255)
                                                                                                                                           aaCACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGc      >  2:4094772/1‑150 (MQ=255)
                                                                                                                                           aaCACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGc      >  1:1230012/1‑150 (MQ=255)
                                                                                                                                            acacCGCAGTCAATAAACCGTCCAGCTCCGCCACCTCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGcc     <  2:487353/150‑1 (MQ=255)
                                                                                                                                             cacCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCa    <  1:4291534/150‑1 (MQ=255)
                                                                                                                                             cacCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCa    >  1:1513708/1‑150 (MQ=255)
                                                                                                                                              acCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAg   >  1:217917/1‑150 (MQ=255)
                                                                                                                                              acCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAg   <  2:4353059/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGC  >  CP009273/1984840‑1985132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑