Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,002,354 C→A 100% L199L (CTC→CTA)  yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,002,3540CA97.8% 926.8 / 7.0 307L199L (CTC→CTA) yedEUPF0394 family sulfur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (148/149);  total (155/152)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 308 total aligned reads displayed.

CTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGA  >  CP009273/2002207‑2002500
                                                                                                                                                   |                                                                                                                                                  
    gCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATg                                                                                                                                              >  2:1028602/1‑150 (MQ=255)
       aTCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTg                                                                                                                                           >  2:4538946/1‑150 (MQ=255)
          gCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTCGGGATGCGGGTc                                                                                                                                        >  2:2982500/1‑150 (MQ=255)
           ccACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTAGAGGGATGCTGGTCt                                                                                                                                       >  2:2394919/1‑150 (MQ=255)
            cACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCtt                                                                                                                                      >  2:4188421/1‑150 (MQ=255)
                gCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTc                                                                                                                                  <  2:1130059/150‑1 (MQ=255)
                gCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTc                                                                                                                                  <  2:3710553/150‑1 (MQ=255)
                 ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                                 <  1:3685670/150‑1 (MQ=255)
                 ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                                 >  1:4832936/1‑150 (MQ=255)
                 ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                                 <  2:3813875/150‑1 (MQ=255)
                 ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCg                                                                                                                                 <  2:436184/150‑1 (MQ=255)
                  cATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                                >  1:2497217/1‑150 (MQ=255)
                  cATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                                <  1:2711662/150‑1 (MQ=255)
                  cATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                                >  1:4632588/1‑150 (MQ=255)
                   aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGAAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTATTTTTAGGc                                                                                                                               >  1:4988772/1‑150 (MQ=255)
                    ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                              >  1:2591196/1‑150 (MQ=255)
                    ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCa                                                                                                                              <  1:4638313/150‑1 (MQ=255)
                        ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgct                                                                                                                          <  2:5016536/150‑1 (MQ=255)
                         tCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgctg                                                                                                                         <  1:3847006/150‑1 (MQ=255)
                          cATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgctgg                                                                                                                        <  2:2705462/150‑1 (MQ=255)
                             ggTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgctgggct                                                                                                                     >  2:2341180/1‑150 (MQ=255)
                                ttGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGctgggctggg                                                                                                                  <  1:2939729/150‑1 (MQ=255)
                                ttGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGctgggctggg                                                                                                                  >  2:4269381/1‑150 (MQ=255)
                                     gcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctg                                                                                                             <  2:1245259/150‑1 (MQ=255)
                                       gcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAGAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctgct                                                                                                           <  1:2134913/150‑1 (MQ=255)
                                       gcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTGTTTTTCGGCATTCTGGGCTGGGCgctgct                                                                                                           >  1:226097/1‑150 (MQ=255)
                                       gcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctgct                                                                                                           >  1:4302742/1‑150 (MQ=255)
                                        cgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTc                                                                                                          <  1:4671487/150‑1 (MQ=255)
                                        cgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTc                                                                                                          >  2:373130/1‑150 (MQ=255)
                                        cgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTc                                                                                                          <  1:5151692/150‑1 (MQ=255)
                                          cTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGGCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTcac                                                                                                        >  2:4911442/1‑150 (MQ=255)
                                                    ctgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                              >  1:4196255/1‑150 (MQ=255)
                                                    ctgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaac                                                                                              <  1:1819560/150‑1 (MQ=255)
                                                     tgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaacc                                                                                             >  1:1667859/1‑150 (MQ=255)
                                                      gcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaacca                                                                                            >  1:1232933/1‑150 (MQ=255)
                                                       cccATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaa                                                                                           >  1:3252940/1‑150 (MQ=255)
                                                       cccATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaa                                                                                           <  2:1688003/150‑1 (MQ=255)
                                                        ccATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaac                                                                                          >  2:725893/1‑150 (MQ=255)
                                                        ccATCTTCCGTATTCCCGTGAAAATGCAGAAAGTGTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTTGGGATGCTGGTCTTTTTCGGCCTGCTGGGCGGGGCGCTGCTCACAGCGATGaaccacc                                                                                          >  2:4764802/1‑148 (MQ=255)
                                                         cATCTTCCGTAGTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaacc                                                                                         <  2:4673178/150‑1 (MQ=255)
                                                          aTCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAAccc                                                                                        >  1:4873426/1‑150 (MQ=255)
                                                           tCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCa                                                                                       <  1:1035897/150‑1 (MQ=255)
                                                           tCTTCCGTAGTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCa                                                                                       <  2:2278551/150‑1 (MQ=255)
                                                            cTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTATGGATGCTGGTCTTTTTCGGCAAGCTGGGCAGGGCGCTGCACAAAGCGATGAACCAACCCaa                                                                                      >  1:634522/1‑150 (MQ=255)
                                                               ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACt                                                                                   <  2:131478/150‑1 (MQ=255)
                                                               ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACt                                                                                   >  2:138723/1‑150 (MQ=255)
                                                                    ttCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGc                                                                              <  1:4491588/150‑1 (MQ=255)
                                                                    ttCCCGTGAAAATGCAGAAAGTTTCAGCAGCCTCAACGCTGACGCAAAAACCGCATCAGGCGCTGCGTCGTTTTCGTCTAAGGATGCTGGTCTTTTTCGGCATTCTTGTCTGGGCGCTGCTCACAGCGATGATCCAACCCACACAGGGGc                                                                              >  2:1781903/1‑150 (MQ=255)
                                                                     tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCt                                                                             <  1:1311930/150‑1 (MQ=255)
                                                                     tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCt                                                                             <  2:309211/150‑1 (MQ=255)
                                                                      cccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGGCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCGGGGCGCTGCTCACAGCGATGAACCAACCCAAACAGGGGCTg                                                                            >  1:3517196/1‑150 (MQ=255)
                                                                      cccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTg                                                                            <  1:2982500/150‑1 (MQ=255)
                                                                      cccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTg                                                                            <  1:3130621/150‑1 (MQ=255)
                                                                          tGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCaa                                                                        <  1:797674/150‑1 (MQ=255)
                                                                            aaaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATg                                                                      >  2:3585820/1‑150 (MQ=255)
                                                                             aaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGc                                                                     >  2:3678681/1‑150 (MQ=255)
                                                                                  cAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGttt                                                                <  1:4260383/150‑1 (MQ=255)
                                                                                  cAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGttt                                                                >  2:1987054/1‑150 (MQ=255)
                                                                                   aGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTg                                                               <  1:2666484/150‑1 (MQ=255)
                                                                                     aaaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGc                                                             >  2:2916571/1‑150 (MQ=255)
                                                                                      aaGTTTCTGCCGCCCCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCg                                                            <  2:287300/150‑1 (MQ=255)
                                                                                       aGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGATGCTGGTCTTTTTCGGCATGCGGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAAATGGGGCTGGCCATGCTGTTTGGCGt                                                           >  1:52624/1‑150 (MQ=255)
                                                                                        gTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGTGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCCCAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTc                                                          <  2:521164/150‑1 (MQ=255)
                                                                                        gTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTc                                                          >  2:1737354/1‑150 (MQ=255)
                                                                                          ttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCgg                                                        >  1:2034774/1‑150 (MQ=255)
                                                                                          ttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCgg                                                        <  1:4172209/150‑1 (MQ=255)
                                                                                                cgccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTgg                                                  >  2:1599692/1‑150 (MQ=255)
                                                                                                   cTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGttt                                               <  1:3792044/150‑1 (MQ=255)
                                                                                                    tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTa                                              <  1:4903698/150‑1 (MQ=255)
                                                                                                     cACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCCAACTGGGGCTGGCACTGCTGTTTGGCGTCGGCTTTGGTTTAc                                             >  2:4014006/1‑150 (MQ=255)
                                                                                                      aCCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACt                                            <  1:918004/150‑1 (MQ=255)
                                                                                                         gCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAt                                         <  2:2229916/150‑1 (MQ=255)
                                                                                                         gCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAt                                         <  1:950885/150‑1 (MQ=255)
                                                                                                         gCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCCAGGGCTGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAt                                         >  1:877152/1‑150 (MQ=255)
                                                                                                          cTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAtt                                        <  2:3551698/150‑1 (MQ=255)
                                                                                                          cTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGAtt                                        <  1:1061578/150‑1 (MQ=255)
                                                                                                               gCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACg                                   <  2:2119348/150‑1 (MQ=255)
                                                                                                                cAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTGTGGCGTCGGCTTTGGTTTACTGATTGAACGt                                  >  2:5055819/1‑150 (MQ=255)
                                                                                                                  aaaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGCTGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgc                                >  1:5124719/1‑150 (MQ=255)
                                                                                                                        ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAAt                          <  2:2539886/150‑1 (MQ=255)
                                                                                                                        ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAAt                          <  1:2339650/150‑1 (MQ=255)
                                                                                                                         gATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATc                         <  2:3582789/150‑1 (MQ=255)
                                                                                                                             aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCt                     >  2:183554/1‑150 (MQ=255)
                                                                                                                             aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAAAGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCAGCt                     >  1:2552334/1‑150 (MQ=255)
                                                                                                                             aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACAGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACAGATTTAACGTGCGCAAATATGCt                     >  1:5107795/1‑150 (MQ=255)
                                                                                                                             aGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAAATGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCt                     >  1:2096666/1‑150 (MQ=255)
                                                                                                                              ggCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCtt                    <  1:4314865/150‑1 (MQ=255)
                                                                                                                                cgcgGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTTGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTa                  >  1:4212709/1‑150 (MQ=255)
                                                                                                                                   ggcgTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACtt               >  2:2504993/1‑150 (MQ=255)
                                                                                                                                      gtcgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAg            >  2:2596731/1‑150 (MQ=255)
                                                                                                                                       tcgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGc           <  1:1392906/150‑1 (MQ=255)
                                                                                                                                        cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg          <  1:1687446/150‑1 (MQ=255)
                                                                                                                                        cgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg          <  1:1557667/150‑1 (MQ=255)
                                                                                                                                         gtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGt         <  2:3326776/150‑1 (MQ=255)
                                                                                                                                          ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGtt        <  2:3859681/150‑1 (MQ=255)
                                                                                                                                            ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTcc      <  1:915274/150‑1 (MQ=255)
                                                                                                                                            ttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTcc      <  1:2694372/150‑1 (MQ=255)
                                                                                                                                               gTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcgcg   >  1:1608812/1‑150 (MQ=255)
                                                                                                                                               gTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcgcg   >  1:3631164/1‑150 (MQ=255)
                                                                                                                                               gTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcgcg   >  1:3981035/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCTGGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCGCGA  >  CP009273/2002207‑2002500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑