BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,041,721	C→A	18.2%	I1101I (ATC→ATA)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,041,721	0	C	A	18.2%	783.1 / 108.3	287	I1101I (ATC→ATA)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base C (96/138); new base A (32/21); total (128/159)
Fisher's exact test for biased strand distribution p-value = 1.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

GAAATCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCA  >  CP009273/2041572‑2041870

gAAATCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATc                                                                                                                                                       <  1:4163594/150‑1 (MQ=255)

 aaaTCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCa                                                                                                                                                      <  2:1752299/150‑1 (MQ=255)

   aTCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACt                                                                                                                                                    >  2:26681/1‑150 (MQ=255)

      aTTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAg                                                                                                                                                 <  1:2559761/150‑1 (MQ=255)

         gggAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGcc                                                                                                                                              <  2:4134530/150‑1 (MQ=255)

         gggAAGGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGcc                                                                                                                                              <  1:3929628/150‑1 (MQ=255)

            aaTGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAAt                                                                                                                                           >  1:1522049/1‑150 (MQ=255)

              tGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATgg                                                                                                                                         >  2:3788074/1‑150 (MQ=255)

                 cGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGa                                                                                                                                      >  2:3060364/1‑150 (MQ=255)

                 cGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGa                                                                                                                                      <  1:1419641/150‑1 (MQ=255)

                 cGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGa                                                                                                                                      >  2:817251/1‑150 (MQ=255)

                  gTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGaa                                                                                                                                     <  1:4831320/150‑1 (MQ=255)

                   tGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAg                                                                                                                                    <  1:1335279/150‑1 (MQ=255)

                    ggATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCCTCACTCAGGCCAATGGGGAAgc                                                                                                                                   >  1:1639532/1‑150 (MQ=255)

                    ggATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAgc                                                                                                                                   <  2:3303566/150‑1 (MQ=255)

                      aTGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAgcgc                                                                                                                                 <  1:3424039/150‑1 (MQ=255)

                      aTGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAgcgc                                                                                                                                 >  1:8954/1‑150 (MQ=255)

                             aaCTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCAc                                                                                                                          <  1:4239030/150‑1 (MQ=255)

                                tctGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCACTGGGGAAGCGCATGTCACGCt                                                                                                                       >  2:2475020/1‑150 (MQ=255)

                                 ctGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTg                                                                                                                      <  1:2081946/150‑1 (MQ=255)

                                     cAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAg                                                                                                                  >  2:406080/1‑150 (MQ=255)

                                      aGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGAGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAgg                                                                                                                 <  1:662492/150‑1 (MQ=255)

                                       gCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGt                                                                                                                >  1:4504820/1‑150 (MQ=255)

                                        cAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTa                                                                                                               <  2:1693883/150‑1 (MQ=255)

                                         aaCAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTaa                                                                                                              >  2:1898538/1‑150 (MQ=255)

                                         aaCAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTaa                                                                                                              <  2:5132654/150‑1 (MQ=255)

                                          aCAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTaaa                                                                                                             <  2:4187915/150‑1 (MQ=255)

                                               gAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGc                                                                                                        >  2:3198683/1‑150 (MQ=255)

                                               gAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGc                                                                                                        >  2:1338569/1‑150 (MQ=255)

                                               gAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGc                                                                                                        <  1:3633109/150‑1 (MQ=255)

                                                aaaGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCg                                                                                                       <  2:439285/150‑1 (MQ=255)

                                                  aGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCggg                                                                                                     <  1:4601078/150‑1 (MQ=255)

                                                   gATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATTCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGc                                                                                                    >  2:3250604/1‑150 (MQ=255)

                                                    aTCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCa                                                                                                   <  1:993318/150‑1 (MQ=255)

                                                    aTCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCa                                                                                                   <  2:2966771/150‑1 (MQ=255)

                                                        gTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCa                                                                                               <  1:5086805/150‑1 (MQ=255)

                                                         tCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCAt                                                                                              <  1:2985726/150‑1 (MQ=255)

                                                           gAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATAc                                                                                            >  1:5067380/1‑150 (MQ=255)

                                                           gAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATAc                                                                                            >  1:595862/1‑150 (MQ=255)

                                                           gAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTGACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATAc                                                                                            <  2:562296/150‑1 (MQ=255)

                                                            aaTCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACg                                                                                           <  2:701556/150‑1 (MQ=255)

                                                              tcatcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGt                                                                                         <  2:2011744/150‑1 (MQ=255)

                                                               catcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGtt                                                                                        >  1:3380147/1‑150 (MQ=255)

                                                                atcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCAAAGGACGTTGCGTCAAACTTTACCCTAGATAATATCGGTATTGCCATAACTCAGGCCTATGGGGATGCGCTCGACACGCTGAAAGGTAATATAGCGGGCACGCATACGGTTa                                                                                       <  2:3664112/150‑1 (MQ=255)

                                                                 tcCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTAc                                                                                      >  2:2404480/1‑150 (MQ=255)

                                                                   cGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCg                                                                                    >  1:4983468/1‑150 (MQ=255)

                                                                    ggTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGc                                                                                   >  2:1804687/1‑150 (MQ=255)

                                                                     gTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCa                                                                                  <  2:304981/150‑1 (MQ=255)

                                                                      tGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCaa                                                                                 >  1:1032288/1‑150 (MQ=255)

                                                                      tGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCaa                                                                                 >  2:1733859/1‑150 (MQ=255)

                                                                      tGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCaa                                                                                 >  1:546060/1‑150 (MQ=255)

                                                                       ggCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAAc                                                                                <  2:273758/150‑1 (MQ=255)

                                                                       ggCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAAc                                                                                <  2:1184474/150‑1 (MQ=255)

                                                                          ggggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCt                                                                             <  2:423265/150‑1 (MQ=255)

                                                                          ggggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCt                                                                             >  2:4661001/1‑150 (MQ=255)

                                                                           gggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTg                                                                            <  2:3035165/150‑1 (MQ=255)

                                                                            ggATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTgg                                                                           <  2:4578779/150‑1 (MQ=255)

                                                                                aaCGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaa                                                                       <  2:1085271/150‑1 (MQ=255)

                                                                                aaCGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaa                                                                       <  2:4479265/150‑1 (MQ=255)

                                                                                  cGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGtaata                                                                     >  2:1454333/1‑150 (MQ=255)

                                                                                    gTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAAc                                                                   >  1:1903417/1‑150 (MQ=255)

                                                                                     tAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACa                                                                  <  1:2218630/150‑1 (MQ=255)

                                                                                       aaCTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAAt                                                                >  2:4851731/1‑150 (MQ=255)

                                                                                          ttCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTAGTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATAcc                                                             <  1:2796516/150‑1 (MQ=255)

                                                                                           tCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCa                                                            >  2:858564/1‑150 (MQ=255)

                                                                                            cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAg                                                           <  1:3201331/150‑1 (MQ=255)

                                                                                            cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAg                                                           >  1:3490173/1‑150 (MQ=255)

                                                                                            cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAg                                                           <  1:4234506/150‑1 (MQ=255)

                                                                                            cACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAg                                                           >  1:4741748/1‑150 (MQ=255)

                                                                                             aCCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGt                                                          >  2:3166548/1‑150 (MQ=255)

                                                                                              ccATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTg                                                         >  1:4568074/1‑150 (MQ=255)

                                                                                               cATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGTAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTCATAACAATACCAGTGa                                                        >  1:1196562/1‑150 (MQ=255)

                                                                                               cATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGa                                                        >  1:4898093/1‑150 (MQ=255)

                                                                                                aTGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGAt                                                       <  1:2933300/150‑1 (MQ=255)

                                                                                                 tGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGAtt                                                      <  2:2232259/150‑1 (MQ=255)

                                                                                                    cacaGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc                                                   >  2:3507590/1‑150 (MQ=255)

                                                                                                    cacaGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGc                                                   >  1:963777/1‑150 (MQ=255)

                                                                                                       aGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGc                                                >  2:4745597/1‑150 (MQ=255)

                                                                                                        ggACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGcc                                               <  1:853042/150‑1 (MQ=255)

                                                                                                        ggACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGcc                                               >  1:1817580/1‑150 (MQ=255)

                                                                                                        ggACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGcc                                               >  1:449679/1‑150 (MQ=255)

                                                                                                              tGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAAc                                         >  1:1075035/1‑150 (MQ=255)

                                                                                                                      aCTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTgg                                 >  2:1162467/1‑150 (MQ=255)

                                                                                                                        tttACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCg                               <  2:3957434/150‑1 (MQ=255)

                                                                                                                        tttACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCg                               <  2:2858146/150‑1 (MQ=255)

                                                                                                                          tACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGa                             >  1:4033498/1‑150 (MQ=255)

                                                                                                                          tACCCTTGAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGa                             >  1:4427203/1‑150 (MQ=255)

                                                                                                                           aCCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGAc                            <  1:698124/150‑1 (MQ=255)

                                                                                                                           aCCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGAc                            <  1:2726827/150‑1 (MQ=255)

                                                                                                                           aCCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGAc                            >  1:3181869/1‑150 (MQ=255)

                                                                                                                                 gAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGcc                      >  2:2272316/1‑150 (MQ=255)

                                                                                                                                 gAAAATAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGcc                      <  1:2125834/150‑1 (MQ=255)

                                                                                                                                   aaaTAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTc                    >  1:2362429/1‑150 (MQ=255)

                                                                                                                                    aaTAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCg                   <  2:2864338/150‑1 (MQ=255)

                                                                                                                                     aTAACGGTATTGCCATAACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCgg                  >  2:2226247/1‑150 (MQ=255)

                                                                                                                                       aaCGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCt                >  1:175494/1‑150 (MQ=255)

                                                                                                                                                  cATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCt     <  1:3788074/150‑1 (MQ=255)

                                                                                                                                                   aTCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTg    >  2:630712/1‑150 (MQ=255)

                                                                                                                                                     cACTCCGGCCAATGGGGACGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCa  <  1:2639365/150‑1 (MQ=255)

                                                                                                                                                     cACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCa  >  2:1584719/1‑150 (MQ=255)

GAAATCATTGGGAATGGCGTGGATGAGACAACTCTGACAGCAACAGTGAAAGATCCGTCGAATCATCCGGTGGCGGGGATAACGGTAAACTTCACCATGCCACAGGACGTTGCGGCAAACTTTACCCTTGAAAATAACGGTATTGCCATCACTCAGGCCAATGGGGAAGCGCATGTCACGCTGAAAGGTAAAAAAGCGGGCACGCATACGGTTACCGCAACGCTGGGTAATAACAATACCAGTGATTCGCAGCCGGTAACATTTGTGGCGGACAAAGCCTCGGCTCAGGTTGTCCTGCA  >  CP009273/2041572‑2041870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`