Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,049,111 C→A 100% T190T (ACC→ACA)  amn → AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,049,1110CA98.9% 1121.3 / 0.9 348T190T (ACC→ACA) amnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (161/182);  total (162/186)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 348 total aligned reads displayed.

GTTAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGGAT  >  CP009273/2048964‑2049257
                                                                                                                                                   |                                                                                                                                                  
 ttAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGt                                                                                                                                                 <  1:3872435/150‑1 (MQ=255)
  tAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                                >  2:4283567/1‑150 (MQ=255)
  tAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                                <  1:1543218/150‑1 (MQ=255)
  tAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTa                                                                                                                                                <  2:3775168/150‑1 (MQ=255)
    aCTCGCCACTACCCGACCACAGAACTCGCGCAAAGTGGCGATGAAACTTCAGACGGCATTTATCATCCAACTGAATACTCCCCGCTATCGCATTTTGATGCGCGCCGCTTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACg                                                                                                                                              <  2:5088623/150‑1 (MQ=255)
      tCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGcc                                                                                                                                            <  1:586926/150‑1 (MQ=255)
        gCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAg                                                                                                                                          >  2:2781890/1‑150 (MQ=255)
          tACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGtt                                                                                                                                        <  1:1008252/150‑1 (MQ=255)
          tACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGtt                                                                                                                                        <  2:1350689/150‑1 (MQ=255)
            cTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCAGTGTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGa                                                                                                                                      >  2:2359762/1‑150 (MQ=255)
             ttCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGaa                                                                                                                                     >  1:3352088/1‑150 (MQ=255)
                ccGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAt                                                                                                                                  <  2:2028286/150‑1 (MQ=255)
                 cGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                                 <  2:2468989/150‑1 (MQ=255)
                 agACAACAGAACTGGCGCAAATTGGCGATGAAACTGCATACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                                 >  2:725544/2‑150 (MQ=255)
                   acaacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtttt                                                                                                                               >  1:1730110/1‑150 (MQ=255)
                      acaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAg                                                                                                                            >  1:784724/1‑150 (MQ=255)
                      acaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAg                                                                                                                            >  1:3618752/1‑150 (MQ=255)
                      acaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAg                                                                                                                            >  2:1292522/1‑150 (MQ=255)
                       caGAACTGGCGCAAATGGGCTATTAAACTGCAGACGGAATTTATCATCAAACTGAATTCGCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACA‑TTTCAGcc                                                                                                                          <  2:2989944/150‑1 (MQ=255)
                        aGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGcc                                                                                                                          >  2:2935300/1‑150 (MQ=255)
                        aGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAAGTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGcc                                                                                                                          <  1:298082/150‑1 (MQ=255)
                         gAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCg                                                                                                                         >  2:3583113/1‑150 (MQ=255)
                            cTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTc                                                                                                                      <  2:1212636/150‑1 (MQ=255)
                            cTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTc                                                                                                                      <  2:2412162/150‑1 (MQ=255)
                              ggCGCAAAGTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGt                                                                                                                    <  1:3139415/150‑1 (MQ=255)
                                cgcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCt                                                                                                                  <  2:4103143/150‑1 (MQ=255)
                                             tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTa                                                                                                     >  1:4928480/1‑150 (MQ=255)
                                             tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTa                                                                                                     >  1:2930586/1‑150 (MQ=255)
                                                aaCTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacac                                                                                                  <  2:174024/150‑1 (MQ=255)
                                                aaCTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacac                                                                                                  >  1:1137510/1‑150 (MQ=255)
                                                    gCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGt                                                                                              <  2:1921300/150‑1 (MQ=255)
                                                      aGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGAGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGTACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                            <  1:3660013/150‑1 (MQ=255)
                                                          ggCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtgtg                                                                                        >  1:2701458/1‑150 (MQ=255)
                                                           gcttttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCGTCGCACGGTTGCGCCATTATACAGGTACGCGAGTTGTACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTgg                                                                                       <  1:3169087/147‑1 (MQ=255)
                                                            cATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGa                                                                                      >  1:3546692/1‑150 (MQ=255)
                                                              tttATCATCCCACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGAATTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATg                                                                                    <  1:2629057/150‑1 (MQ=255)
                                                               ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGa                                                                                   >  1:3049658/1‑150 (MQ=255)
                                                               ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGa                                                                                   >  2:4360712/1‑150 (MQ=255)
                                                                      ccAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                            >  2:1540293/1‑150 (MQ=255)
                                                                       cAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttc                                                                           <  1:1071649/150‑1 (MQ=255)
                                                                        aaCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttcg                                                                          <  1:3955096/150‑1 (MQ=255)
                                                                           tGAATTCTCCCCGCTATAGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGGTCGTTg                                                                       <  1:4791184/150‑1 (MQ=255)
                                                                            gAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgg                                                                      >  2:390331/1‑150 (MQ=255)
                                                                            gAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgg                                                                      >  2:924308/1‑150 (MQ=255)
                                                                             aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                     >  1:468156/1‑150 (MQ=255)
                                                                             aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                     >  1:926650/1‑150 (MQ=255)
                                                                             aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                     <  1:3252231/150‑1 (MQ=255)
                                                                             aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                     <  2:1238168/150‑1 (MQ=255)
                                                                               ttCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGa                                                                   >  2:2545117/1‑150 (MQ=255)
                                                                                tctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGAt                                                                  >  2:2336474/1‑150 (MQ=255)
                                                                                 ctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATg                                                                 <  1:3274261/150‑1 (MQ=255)
                                                                                   ccccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCa                                                               >  1:2848752/1‑150 (MQ=255)
                                                                                      cGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGcc                                                            <  2:1593471/150‑1 (MQ=255)
                                                                                      cGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGcc                                                            >  2:3430437/1‑150 (MQ=255)
                                                                                       gCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCa                                                           <  1:3726020/150‑1 (MQ=255)
                                                                                        cTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAg                                                          <  1:1099257/150‑1 (MQ=255)
                                                                                         tATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGa                                                         >  2:1803422/1‑150 (MQ=255)
                                                                                         tATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGGTCGTTGGGGATGCAGCCAGa                                                         >  2:4377743/1‑150 (MQ=255)
                                                                                             gCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCt                                                     <  2:403314/150‑1 (MQ=255)
                                                                                             gCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCt                                                     <  2:4597664/150‑1 (MQ=255)
                                                                                              cATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTc                                                    >  1:1271807/1‑150 (MQ=255)
                                                                                                ttttGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATGCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACCCGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGa                                                  <  2:523888/150‑1 (MQ=255)
                                                                                                   tGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATcc                                               >  1:1461617/1‑150 (MQ=255)
                                                                                                   tGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATcc                                               >  1:2014475/1‑150 (MQ=255)
                                                                                                   tGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATcc                                               >  1:2563053/1‑150 (MQ=255)
                                                                                                    gATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCt                                              <  2:3049774/150‑1 (MQ=255)
                                                                                                    gATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCt                                              <  1:4145996/150‑1 (MQ=255)
                                                                                                    gATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCt                                              >  1:4082538/1‑150 (MQ=255)
                                                                                                      tGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGa                                            <  2:4993789/150‑1 (MQ=255)
                                                                                                       gcgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGAt                                           <  2:2133329/150‑1 (MQ=255)
                                                                                                        cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATa                                          <  2:5108308/150‑1 (MQ=255)
                                                                                                        cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATa                                          <  1:2124962/150‑1 (MQ=255)
                                                                                                        cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATa                                          <  1:2863921/150‑1 (MQ=255)
                                                                                                        cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATa                                          >  2:3131663/1‑150 (MQ=255)
                                                                                                           gccgcGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTc                                       >  2:4729915/1‑150 (MQ=255)
                                                                                                              gcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCt                                    <  1:2253840/150‑1 (MQ=255)
                                                                                                               cgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTa                                   >  2:483907/1‑150 (MQ=255)
                                                                                                                gTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTAc                                  >  2:2255926/1‑150 (MQ=255)
                                                                                                                gTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTAc                                  >  1:1655540/1‑150 (MQ=255)
                                                                                                                gTCGATTTGTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTAc                                  <  1:1433745/150‑1 (MQ=255)
                                                                                                                   gATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAtt                               >  2:37972/1‑150 (MQ=255)
                                                                                                                   gATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACAtt                               <  1:1367122/150‑1 (MQ=255)
                                                                                                                      ttttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGcc                            >  1:52243/1‑150 (MQ=255)
                                                                                                                      ttttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGcc                            <  1:2389840/150‑1 (MQ=255)
                                                                                                                         tCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCtt                         >  1:3996846/1‑150 (MQ=255)
                                                                                                                         tCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCtt                         >  1:4270354/1‑150 (MQ=255)
                                                                                                                          cccTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCttt                        <  2:80392/150‑1 (MQ=255)
                                                                                                                           ccTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTc                       <  2:877309/150‑1 (MQ=255)
                                                                                                                             tCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCtt                     <  2:3532672/150‑1 (MQ=255)
                                                                                                                             tCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCtt                     <  1:4495651/150‑1 (MQ=255)
                                                                                                                              cGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtg                    >  2:3669410/1‑150 (MQ=255)
                                                                                                                               gCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtgt                   >  2:4694264/1‑150 (MQ=255)
                                                                                                                                 aCGGTTGCGCCATTATACTGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGc                 <  2:1104777/150‑1 (MQ=255)
                                                                                                                                  cGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCt                <  2:4892426/150‑1 (MQ=255)
                                                                                                                                  cGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCt                <  1:1804821/150‑1 (MQ=255)
                                                                                                                                      tGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCg            <  1:3346047/150‑1 (MQ=255)
                                                                                                                                          ccATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGaa        <  2:2559675/150‑1 (MQ=255)
                                                                                                                                              tataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTgg    >  1:1617695/1‑150 (MQ=255)
                                                                                                                                               ataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGGa   >  2:4651729/1‑150 (MQ=255)
                                                                                                                                               ataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGGa   >  1:1719545/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGGAT  >  CP009273/2048964‑2049257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑