Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,118,194 A→T 5.5% L345Q (CTG→CAG)  wcaI ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,118,1940AT 5.5% 989.5 / 32.8 311L345Q (CTG→CAG) wcaIputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (164/130);  new base T (6/11);  total (170/141)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 314 total aligned reads displayed.

CTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTC  >  CP009273/2118045‑2118342
                                                                                                                                                     |                                                                                                                                                    
ctTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCa                                                                                                                                                      <  1:3046538/150‑1 (MQ=255)
 tttATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAg                                                                                                                                                     >  1:4528741/1‑150 (MQ=255)
   tATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGtt                                                                                                                                                   >  1:3373947/1‑150 (MQ=255)
   tATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGtt                                                                                                                                                   <  2:203283/150‑1 (MQ=255)
    aTCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCTGTTc                                                                                                                                                  >  2:1295915/1‑150 (MQ=255)
    aTCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTc                                                                                                                                                  >  1:5144320/1‑150 (MQ=255)
     tCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCt                                                                                                                                                 >  1:842549/1‑150 (MQ=255)
      cGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGACAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCtg                                                                                                                                                >  1:10073/1‑150 (MQ=255)
          cgtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTa                                                                                                                                            >  1:3907514/1‑150 (MQ=255)
           gtgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAg                                                                                                                                           >  1:5019588/1‑150 (MQ=255)
            tgcgtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAgg                                                                                                                                          >  2:1435972/1‑150 (MQ=255)
               gtTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCtt                                                                                                                                       >  2:4572249/1‑150 (MQ=255)
                 tCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTca                                                                                                                                     >  2:22945/1‑150 (MQ=255)
                  cGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCTGTTCTGTGTAGGCTTcag                                                                                                                                    <  2:2113236/150‑1 (MQ=255)
                  cGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTcag                                                                                                                                    >  1:4646230/1‑150 (MQ=255)
                    gCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTcagca                                                                                                                                  >  1:795724/1‑150 (MQ=255)
                     cATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTcagcag                                                                                                                                 >  2:4436028/1‑150 (MQ=255)
                     cATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTcagcag                                                                                                                                 >  2:3651466/1‑150 (MQ=255)
                     cATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTcagcag                                                                                                                                 >  2:2222835/1‑150 (MQ=255)
                      atatTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGt                                                                                                                                >  1:12035/1‑150 (MQ=255)
                      atatTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGt                                                                                                                                >  1:1118821/1‑150 (MQ=255)
                      atatTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGt                                                                                                                                >  2:5007012/1‑150 (MQ=255)
                         ttCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCTGTTCTGTGTAGGCTTCAGCAGTAAt                                                                                                                             <  1:1341003/150‑1 (MQ=255)
                          tCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATc                                                                                                                            <  1:12553/150‑1 (MQ=255)
                           cACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCa                                                                                                                           >  2:328993/1‑150 (MQ=255)
                            aCGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCAc                                                                                                                          <  2:1126378/150‑1 (MQ=255)
                             cGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCAcc                                                                                                                         >  2:133368/1‑150 (MQ=255)
                                gCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACcgcg                                                                                                                      >  2:3764089/1‑150 (MQ=255)
                                gCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACcgcg                                                                                                                      <  2:5158163/150‑1 (MQ=255)
                                  cACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGtt                                                                                                                    <  2:653363/150‑1 (MQ=255)
                                  cACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGtt                                                                                                                    <  2:3413280/150‑1 (MQ=255)
                                  cACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGtt                                                                                                                    <  2:4263806/150‑1 (MQ=255)
                                     cGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTAcc                                                                                                                 >  2:3854573/1‑150 (MQ=255)
                                       tgttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGc                                                                                                               >  1:3694105/1‑150 (MQ=255)
                                       tgttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGc                                                                                                               >  2:829579/1‑150 (MQ=255)
                                         ttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCt                                                                                                             <  2:950715/150‑1 (MQ=255)
                                         ttgtgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCt                                                                                                             <  1:746863/150‑1 (MQ=255)
                                            tgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACt                                                                                                          <  2:1503664/150‑1 (MQ=255)
                                            tgttTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACt                                                                                                          >  2:632434/1‑150 (MQ=255)
                                                tGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCa                                                                                                      >  1:3593074/1‑150 (MQ=255)
                                                       cagGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATAtt                                                                                               <  2:168802/150‑1 (MQ=255)
                                                         ggAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTgg                                                                                             <  1:1064578/150‑1 (MQ=255)
                                                              gcCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCTGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGt                                                                                        <  1:3987938/150‑1 (MQ=255)
                                                               ccTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGtt                                                                                       >  2:4354626/1‑150 (MQ=255)
                                                                cTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCTGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGttt                                                                                      >  2:4111729/1‑150 (MQ=255)
                                                                cTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGGTACCGCCTACTGCGAGACTATTGGTCAGttt                                                                                      >  2:4660378/1‑150 (MQ=255)
                                                                 tGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTc                                                                                     <  2:4888167/150‑1 (MQ=255)
                                                                  gACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCg                                                                                    <  2:3528230/150‑1 (MQ=255)
                                                                   aCGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGa                                                                                   <  2:4148542/150‑1 (MQ=255)
                                                                   aCGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGa                                                                                   <  2:4735281/150‑1 (MQ=255)
                                                                    cGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCTGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGAc                                                                                  <  2:2934469/150‑1 (MQ=255)
                                                                        tCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCa                                                                              <  2:546817/150‑1 (MQ=255)
                                                                            cGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATAc                                                                          <  1:4387006/150‑1 (MQ=255)
                                                                                accaGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCACTACGGCa                                                                      >  2:2476170/1‑150 (MQ=255)
                                                                                  caGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATc                                                                    <  1:65671/150‑1 (MQ=255)
                                                                                       ccTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTgcca                                                               <  1:144346/150‑2 (MQ=255)
                                                                                        cTCGACCGAGTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTgccgc                                                              <  1:1330970/150‑1 (MQ=255)
                                                                                         tCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTgccgcg                                                             >  1:174588/1‑150 (MQ=255)
                                                                                                aTTCCGGTTCAACGCAAACCGCAATGCCCGGACAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGtt                                                      >  1:4285255/1‑150 (MQ=255)
                                                                                                aTTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGtt                                                      <  1:3789624/150‑1 (MQ=255)
                                                                                                aTTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGtt                                                      <  1:4436028/150‑1 (MQ=255)
                                                                                                 ttCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGttt                                                     <  1:3269861/150‑1 (MQ=255)
                                                                                                       ttCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGcac                                               <  1:4144923/150‑1 (MQ=255)
                                                                                                          aaCGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGcaccac                                            >  1:2412838/1‑150 (MQ=255)
                                                                                                            cGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCAccacca                                          <  2:1225776/150‑1 (MQ=255)
                                                                                                               aaaCCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGAt                                       >  1:594434/1‑150 (MQ=255)
                                                                                                               aaaCCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAAGCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGAt                                       <  1:1506201/150‑1 (MQ=255)
                                                                                                                aaCCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATg                                      <  2:1639917/150‑1 (MQ=255)
                                                                                                                   cGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCa                                   <  1:2377791/150‑1 (MQ=255)
                                                                                                                   cGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCa                                   <  2:10073/150‑1 (MQ=255)
                                                                                                                    gCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCaa                                  <  2:2817195/150‑1 (MQ=255)
                                                                                                                    gCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCaa                                  <  2:181431/150‑1 (MQ=255)
                                                                                                                    gCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCaa                                  <  1:3145887/150‑1 (MQ=255)
                                                                                                                     cAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAAt                                 >  2:2545180/1‑150 (MQ=255)
                                                                                                                     cAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAAt                                 >  1:242634/1‑150 (MQ=255)
                                                                                                                      aaTGCCCGGAAAGGTTTCGCAAAGCTGCCCCATTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTTGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATc                                <  1:1929430/150‑1 (MQ=255)
                                                                                                                      aaTGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATc                                >  2:4694684/1‑150 (MQ=255)
                                                                                                                      aaTGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATc                                <  1:906976/150‑1 (MQ=255)
                                                                                                                       aTGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCg                               <  1:22945/150‑1 (MQ=255)
                                                                                                                        tGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGc                              >  2:3183709/1‑150 (MQ=255)
                                                                                                                          cccGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGccc                            >  2:3103040/1‑150 (MQ=255)
                                                                                                                           ccGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCa                           >  2:711643/1‑150 (MQ=255)
                                                                                                                              gAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCt                        <  1:1255031/150‑1 (MQ=255)
                                                                                                                               aaaGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCtt                       <  2:3568367/150‑1 (MQ=255)
                                                                                                                               aaaGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCtt                       >  1:2939436/1‑150 (MQ=255)
                                                                                                                                   gTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTcagc                   <  2:775564/150‑1 (MQ=255)
                                                                                                                                   gTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTcagc                   <  2:795834/150‑1 (MQ=255)
                                                                                                                                   gTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTcagc                   >  1:4491896/1‑150 (MQ=255)
                                                                                                                                     ttCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTcagcag                 >  1:1574261/1‑150 (MQ=255)
                                                                                                                                       cGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTg               <  1:2705045/150‑1 (MQ=255)
                                                                                                                                        gCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGc              <  1:284414/150‑1 (MQ=255)
                                                                                                                                        gCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGc              <  2:1414112/150‑1 (MQ=255)
                                                                                                                                         cAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCg             >  1:4802796/1‑150 (MQ=255)
                                                                                                                                          aaaGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCgg            >  2:3266750/1‑150 (MQ=255)
                                                                                                                                          aaaGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCgg            >  1:2596595/1‑150 (MQ=255)
                                                                                                                                                gCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAg      <  1:2029304/150‑1 (MQ=255)
                                                                                                                                                 ccccAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGc     <  1:3490709/150‑1 (MQ=255)
                                                                                                                                                 ccccAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTCTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGc     <  1:219083/150‑1 (MQ=255)
                                                                                                                                                  cccAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCg    <  2:4185183/150‑1 (MQ=255)
                                                                                                                                                    cAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTc  >  1:1849777/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTTATCGAGCGTGCGTTCGGCATATTCACGTGCCACCGTGTTGTGTTTGGGCAGCAGGAGCGCCTGACGGATCCCCGCCACCAGCGCCTCGACCGATTCCGGTTCAACGCAAACCGCAATGCCCGGAAAGGTTTCGCAAAGCTGCCCCAGTTCTGTGTAGGCTTCAGCAGTAATCACCGCGTTACCGCCTACTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTC  >  CP009273/2118045‑2118342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑