Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,194,695 T→A 100% L313Q (CTA→CAA)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,194,6950TA99.7% 1094.1 / ‑4.9 328L313Q (CTA→CAA) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (160/167);  total (160/168)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 328 total aligned reads displayed.

AAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA  >  CP009273/2194549‑2194841
                                                                                                                                                  |                                                                                                                                                  
aaaCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACt                                                                                                                                                 >  1:3678972/1‑150 (MQ=255)
  aCCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtgg                                                                                                                                               <  1:3022311/150‑1 (MQ=255)
    cACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtg                                                                                                                                             >  1:4899655/1‑150 (MQ=255)
     aCTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACtggtgg                                                                                                                                            >  2:4495162/1‑150 (MQ=255)
      cTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGc                                                                                                                                           >  1:4590644/1‑150 (MQ=255)
        tCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTg                                                                                                                                         >  1:922123/1‑150 (MQ=255)
         cGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGa                                                                                                                                        >  2:1033490/1‑150 (MQ=255)
         cGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGa                                                                                                                                        >  2:1459502/1‑150 (MQ=255)
          ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGAt                                                                                                                                       >  1:5157121/1‑150 (MQ=255)
          ggCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGAt                                                                                                                                       >  1:4863508/1‑150 (MQ=255)
             tACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                    >  2:4895824/1‑150 (MQ=255)
             tACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaa                                                                                                                                    >  1:235521/1‑150 (MQ=255)
              aCGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaa                                                                                                                                   <  1:2258853/150‑1 (MQ=255)
              aCGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATaaaa                                                                                                                                   >  1:5070745/1‑150 (MQ=255)
                 cgGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAaaaca                                                                                                                                >  2:3075716/1‑150 (MQ=255)
                     cTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAAcc                                                                                                                            >  1:1607091/1‑150 (MQ=255)
                      tGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAAccc                                                                                                                           <  1:1942676/150‑1 (MQ=255)
                       gcgcCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCa                                                                                                                          <  2:3258903/150‑1 (MQ=255)
                       gcgcCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCa                                                                                                                          <  2:1383342/150‑1 (MQ=255)
                       gcgcCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCa                                                                                                                          <  1:2023616/150‑1 (MQ=255)
                          ccgccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGcc                                                                                                                       <  2:384148/150‑1 (MQ=255)
                           cgccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCt                                                                                                                      <  1:1663516/150‑1 (MQ=255)
                            gccCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTg                                                                                                                     <  1:1496426/150‑1 (MQ=255)
                              ccTTTTGCGCGAACAAGGACTGGTAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                                                   <  2:2006759/150‑1 (MQ=255)
                              ccTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCa                                                                                                                   <  1:1716167/150‑1 (MQ=255)
                               cTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATGAAAACAAACCCAGCCTGCaa                                                                                                                  >  2:1167626/1‑150 (MQ=255)
                                 tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                                <  2:2768107/150‑1 (MQ=255)
                                 tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                                <  2:1801570/150‑1 (MQ=255)
                                 tttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACg                                                                                                                <  2:2076725/150‑1 (MQ=255)
                                  ttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgt                                                                                                               <  1:4724960/150‑1 (MQ=255)
                                  ttGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgt                                                                                                               <  1:4061801/150‑1 (MQ=255)
                                    gcgcGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACgtgt                                                                                                             >  1:4267283/1‑150 (MQ=255)
                                     cgcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTa                                                                                                            <  1:2977699/150‑1 (MQ=255)
                                     cgcgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTa                                                                                                            <  1:630992/150‑1 (MQ=255)
                                       cgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGc                                                                                                          <  1:830173/150‑1 (MQ=255)
                                       cgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGc                                                                                                          >  1:4518141/1‑150 (MQ=255)
                                       cgAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGc                                                                                                          >  2:2852285/1‑150 (MQ=255)
                                        gAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCt                                                                                                         >  2:4149251/1‑150 (MQ=255)
                                         aaCAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTa                                                                                                        >  1:2782282/1‑150 (MQ=255)
                                            aaGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAAt                                                                                                     <  1:1255395/150‑1 (MQ=255)
                                             aGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATa                                                                                                    <  1:4030754/150‑1 (MQ=255)
                                               gACTGGCAGCCATTCCGCGCCTCGCGATGTATGACCATAAAGAAGATTGCGGCAGTCTGCTGTTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCACCGGGTAGCTCAATAcc                                                                                                  >  2:4684793/1‑150 (MQ=255)
                                                   ggCAGCCAGTCCGCGCCTCGCGATGTATGCCCATAAAGAAGAGTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaa                                                                                              <  1:4396814/150‑1 (MQ=255)
                                                     cAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaaa                                                                                            <  1:390918/150‑1 (MQ=255)
                                                      agacATTCCGAGCCTCGAGATTTATGCCCATAAAGAAGATTGCTGAATTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGTCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATaaaaa                                                                                           <  2:2193740/147‑1 (MQ=255)
                                                        ccATTCCGCGCCTCGCGATGTATGCCCATAACGAAGATTGCGGCAGTCTGCTGTTACAAATTAACCATCCGCAAGTCATCCGCACCTTGAAACTGGTGGCTGATAAAAACAAACCCATCCTGCAACGTGTAGCTAAATACCATAAAAACt                                                                                         <  2:724931/150‑1 (MQ=255)
                                                           ttCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcc                                                                                      <  1:3985253/150‑1 (MQ=255)
                                                           ttCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTcc                                                                                      <  1:3952804/150‑1 (MQ=255)
                                                            tCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTccc                                                                                     >  2:3440670/1‑150 (MQ=255)
                                                                cgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCAt                                                                                 >  2:2042114/1‑150 (MQ=255)
                                                                cgcCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCAGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCAt                                                                                 >  1:3386140/1‑150 (MQ=255)
                                                                  ccTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGc                                                                               >  1:2405826/1‑150 (MQ=255)
                                                                  ccTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGc                                                                               <  2:1535535/150‑1 (MQ=255)
                                                                   cTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAACACTTCCCCCATGCg                                                                              <  2:4986693/150‑1 (MQ=255)
                                                                   cTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCg                                                                              <  2:2488788/150‑1 (MQ=255)
                                                                    tCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGa                                                                             <  1:2687776/150‑1 (MQ=255)
                                                                        gATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCt                                                                         >  2:2906634/1‑150 (MQ=255)
                                                                          tGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACACATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcg                                                                       <  2:5162270/150‑1 (MQ=255)
                                                                          tGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcg                                                                       >  1:2121656/1‑150 (MQ=255)
                                                                             aTGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTcgccg                                                                    <  2:3169634/150‑1 (MQ=255)
                                                                                 ccATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACt                                                                >  1:1982837/1‑150 (MQ=255)
                                                                                   aTAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                              >  2:4019238/1‑150 (MQ=255)
                                                                                   aTAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                              >  1:3072724/1‑150 (MQ=255)
                                                                                   aTAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTgg                                                              >  2:1130760/1‑150 (MQ=255)
                                                                                          agaTTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAAct                                                       <  2:2439376/150‑1 (MQ=255)
                                                                                             ttGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAActgct                                                    <  1:3188428/150‑1 (MQ=255)
                                                                                                tggCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGc                                                 >  2:4275532/2‑150 (MQ=255)
                                                                                                cGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGc                                                 <  1:4652348/150‑1 (MQ=255)
                                                                                                  gCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGt                                               >  2:2547189/1‑150 (MQ=255)
                                                                                                   cAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGtt                                              <  2:2349663/150‑1 (MQ=255)
                                                                                                   cAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGtt                                              <  1:4135196/150‑1 (MQ=255)
                                                                                                    aGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTa                                             <  2:2268166/150‑1 (MQ=255)
                                                                                                    aGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTa                                             <  2:1245915/150‑1 (MQ=255)
                                                                                                      tCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAAc                                           >  2:3392700/1‑150 (MQ=255)
                                                                                                       ctgctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACa                                          <  2:1665227/150‑1 (MQ=255)
                                                                                                          ctgGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGaa                                       >  1:1276785/1‑150 (MQ=255)
                                                                                                                cAAATTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAAccacca                                 <  1:4337653/150‑1 (MQ=255)
                                                                                                                 aaaTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGACCCACCAg                                >  2:457790/1‑150 (MQ=255)
                                                                                                                 aaaTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAg                                <  1:5114188/150‑1 (MQ=255)
                                                                                                                   aTTAACCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcc                              <  2:850948/150‑1 (MQ=255)
                                                                                                                       aCCATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgcc                          >  2:4377261/1‑150 (MQ=255)
                                                                                                                        ccATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAgcccgccc                         <  1:4203294/150‑1 (MQ=255)
                                                                                                                         cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                        <  1:714682/150‑1 (MQ=255)
                                                                                                                         cATCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCt                        <  2:3306719/150‑1 (MQ=255)
                                                                                                                          aTCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTg                       <  1:1211273/150‑1 (MQ=255)
                                                                                                                          aTCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTg                       <  2:5088323/150‑1 (MQ=255)
                                                                                                                          aTCCGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTg                       <  1:3317869/150‑1 (MQ=255)
                                                                                                                            ccGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGt                     >  1:35005/1‑150 (MQ=255)
                                                                                                                             cGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGtt                    >  2:1308672/1‑150 (MQ=255)
                                                                                                                             cGCAAGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGtt                    >  2:4735824/1‑150 (MQ=255)
                                                                                                                                aaGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATc                 >  2:2593982/1‑150 (MQ=255)
                                                                                                                                 aGTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATcc                >  1:1466762/1‑150 (MQ=255)
                                                                                                                                  gTCATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCa               <  1:1044489/150‑1 (MQ=255)
                                                                                                                                    cATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAACACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAat             <  1:3557999/150‑1 (MQ=255)
                                                                                                                                    cATCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAat             <  2:4334267/150‑1 (MQ=255)
                                                                                                                                     aTCCGCACCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatc            >  2:2755891/1‑150 (MQ=255)
                                                                                                                                      tCCGCACCTCGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAatca           >  1:3233995/1‑150 (MQ=255)
                                                                                                                                           aCCTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGaa      >  2:680309/1‑150 (MQ=255)
                                                                                                                                            ccTTGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAg     >  1:568631/1‑150 (MQ=255)
                                                                                                                                              ttGCAACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGAc   >  2:3005234/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAACCACTTCGGCTACGCGGCCTGCGCCGCCCTTTTGCGCGAACAAGGACTGGCAGCCATTCCGCGCCTCGCGATGTATGCCCATAAAGAAGATTGCGGCAGTCTGCTGGTACAAATTAACCATCCGCAAGTCATCCGCACCTTGCTACTGGTGGCTGATAAAAACAAACCCAGCCTGCAACGTGTAGCTAAATACCATAAAAACTTCCCCCATGCGACGCTCGCCGCACTGGCAGAACTGCTGGCGTTAACAGAACCACCAGCCCGCCCTGGTTATCCAATCATCGAAGACA  >  CP009273/2194549‑2194841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑