Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,216,296 C→A 100% I211I (ATC→ATA)  dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,216,2960CA99.2% 1081.5 / ‑1.7 330I211I (ATC→ATA) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (154/173);  total (155/175)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 333 total aligned reads displayed.

GCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT  >  CP009273/2216149‑2216442
                                                                                                                                                   |                                                                                                                                                  
gCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATaaa                                                                                                                                                  >  2:5090610/1‑150 (MQ=255)
     tGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATg                                                                                                                                             >  1:1739029/1‑150 (MQ=255)
      gCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGa                                                                                                                                            <  1:917602/150‑1 (MQ=255)
      gCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGa                                                                                                                                            <  2:1361056/150‑1 (MQ=255)
      gCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGa                                                                                                                                            <  2:3796831/150‑1 (MQ=255)
       cAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGAc                                                                                                                                           <  1:4763424/150‑1 (MQ=255)
        aaCGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACg                                                                                                                                          <  2:2047068/150‑1 (MQ=255)
          cGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAt                                                                                                                                        <  2:2225333/150‑1 (MQ=255)
             ggCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGAtgtg                                                                                                                                     <  2:3514070/150‑1 (MQ=255)
              gCCCGGCTTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTTATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGc                                                                                                                                    <  1:1601716/150‑1 (MQ=255)
                 cGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTc                                                                                                                                 >  1:2868094/1‑150 (MQ=255)
                 cGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTc                                                                                                                                 >  1:274244/1‑150 (MQ=255)
                 cGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTc                                                                                                                                 >  1:3034857/1‑150 (MQ=255)
                  ggCGTATACCTACCTCTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCa                                                                                                                                <  2:2681855/150‑1 (MQ=255)
                  ggCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCa                                                                                                                                >  1:2917578/1‑150 (MQ=255)
                  ggCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCa                                                                                                                                >  2:3598152/1‑150 (MQ=255)
                    cGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACg                                                                                                                              >  2:3425481/1‑150 (MQ=255)
                     gTATACCGAAATGTCGTTATTCGAGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGa                                                                                                                             >  2:1746967/1‑150 (MQ=255)
                      tataCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGAt                                                                                                                            <  2:1024267/150‑1 (MQ=255)
                       ataCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATg                                                                                                                           >  1:144787/1‑150 (MQ=255)
                        taCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATgg                                                                                                                          >  2:601231/1‑150 (MQ=255)
                         aCCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgt                                                                                                                         <  2:2432660/150‑1 (MQ=255)
                          ccGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtc                                                                                                                        <  2:3936558/150‑1 (MQ=255)
                           cGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcg                                                                                                                       >  1:1539731/1‑150 (MQ=255)
                             aaaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                     <  1:3352473/150‑1 (MQ=255)
                             aaaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                     >  2:4617809/1‑150 (MQ=255)
                             aaaTGTCGTGATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGgtcgtc                                                                                                                     <  1:3938386/150‑1 (MQ=255)
                              aaTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCa                                                                                                                    <  1:3304869/150‑1 (MQ=255)
                               aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCAc                                                                                                                   <  1:4505791/150‑1 (MQ=255)
                               aTGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCAc                                                                                                                   <  1:4062673/150‑1 (MQ=255)
                                tGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACg                                                                                                                  >  1:1119707/1‑150 (MQ=255)
                                   cGTTATTCGCGCGTATAAATGAAGCCGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGccc                                                                                                               >  1:3851942/1‑150 (MQ=255)
                                    gTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCa                                                                                                              >  1:265028/1‑150 (MQ=255)
                                     ttATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAc                                                                                                             >  1:5015158/1‑150 (MQ=255)
                                     ttATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAc                                                                                                             <  2:2789550/150‑1 (MQ=255)
                                     ttATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCAc                                                                                                             <  2:925426/150‑1 (MQ=255)
                                      tATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACg                                                                                                            >  1:3443529/1‑150 (MQ=255)
                                      tATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACg                                                                                                            <  2:1592380/150‑1 (MQ=255)
                                        ttCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGAt                                                                                                          <  2:4032004/150‑1 (MQ=255)
                                          cgcgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTa                                                                                                        <  1:2999899/150‑1 (MQ=255)
                                            cgcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATg                                                                                                      >  1:4137075/1‑150 (MQ=255)
                                             gcgTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGa                                                                                                     >  2:2584314/1‑150 (MQ=255)
                                                  taAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATg                                                                                                >  2:415673/1‑150 (MQ=255)
                                                   aaaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGt                                                                                               <  1:2310973/150‑1 (MQ=255)
                                                    aaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTc                                                                                              <  2:2167293/150‑1 (MQ=255)
                                                    aaTGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTc                                                                                              <  2:1510895/150‑1 (MQ=255)
                                                       gAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCAc                                                                                           >  1:824741/1‑150 (MQ=255)
                                                          gACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACcgc                                                                                        >  1:3538160/1‑150 (MQ=255)
                                                              gCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTc                                                                                    <  1:4532229/150‑1 (MQ=255)
                                                              gCAAACTGACGCTGGTGAACAATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTc                                                                                    <  1:2548631/150‑1 (MQ=255)
                                                                aaaCTGAAGCTTGTGAAACAGATGGGGATTGATCTGGTCTAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAATAGATGAAGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGt                                                                                  <  1:531850/150‑1 (MQ=255)
                                                                  aCTGACGCTGGTGATCCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATACTTAGAAAGATGGATGATGATCGCATAAAAGATGAAGATGTGCGTCACGATGGTCGTCAAGCCCACGATTATGACTATGTCCACCGCGTTCGTGa                                                                                <  1:942417/150‑1 (MQ=255)
                                                                   cTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGat                                                                               <  1:2434633/150‑1 (MQ=255)
                                                                   cTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGat                                                                               <  2:1687876/150‑1 (MQ=255)
                                                                      aCGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATAtt                                                                            <  2:1207737/150‑1 (MQ=255)
                                                                      aCGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATAtt                                                                            <  2:830862/150‑1 (MQ=255)
                                                                       cGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTg                                                                           >  2:3348504/1‑150 (MQ=255)
                                                                       cGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTg                                                                           <  1:63449/150‑1 (MQ=255)
                                                                        gCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGa                                                                          <  1:3241452/150‑1 (MQ=255)
                                                                         cTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGaa                                                                         <  1:4455430/150‑1 (MQ=255)
                                                                          tGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAg                                                                        >  2:2313555/1‑150 (MQ=255)
                                                                          tGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAg                                                                        <  2:4792612/150‑1 (MQ=255)
                                                                                  cATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACg                                                                <  1:1639689/150‑1 (MQ=255)
                                                                                   aTCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGc                                                               <  1:3740241/150‑1 (MQ=255)
                                                                                     cTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGccc                                                             >  1:4288533/1‑150 (MQ=255)
                                                                                     cTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGccc                                                             <  1:2001647/150‑1 (MQ=255)
                                                                                        gggATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCa                                                          <  1:4939585/150‑1 (MQ=255)
                                                                                          gATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACg                                                        <  2:4329892/150‑1 (MQ=255)
                                                                                            ttGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGtt                                                      >  1:148764/1‑150 (MQ=255)
                                                                                            ttGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGtt                                                      <  2:2552205/150‑1 (MQ=255)
                                                                                            ttGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGtt                                                      >  2:2975322/1‑150 (MQ=255)
                                                                                               aTCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTtata                                                   <  2:1135566/150‑1 (MQ=255)
                                                                                                   gggCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGc                                               >  1:4809946/1‑150 (MQ=255)
                                                                                                         aaCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATcc                                         <  1:1045603/150‑1 (MQ=255)
                                                                                                          aCGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGAGATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCt                                        >  2:756714/1‑150 (MQ=255)
                                                                                                           cGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTg                                       >  2:4252064/1‑150 (MQ=255)
                                                                                                               ggAGCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGACCGCACGTTATAACGCCGATCCTGATCg                                   >  2:2903120/1‑150 (MQ=255)
                                                                                                                  gCAGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGtt                                >  2:3677334/1‑150 (MQ=255)
                                                                                                                    aGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                              >  2:1777975/1‑150 (MQ=255)
                                                                                                                    aGATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAt                              >  1:2172514/1‑150 (MQ=255)
                                                                                                                     gATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                             >  2:4950394/1‑150 (MQ=255)
                                                                                                                     gATCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAtt                             <  2:3724320/150‑1 (MQ=255)
                                                                                                                      aTCCTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTAttt                            >  1:1038481/1‑150 (MQ=255)
                                                                                                                        ccTTAGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGa                          <  2:4501434/150‑1 (MQ=255)
                                                                                                                            aGCAAGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtct                      >  1:3070622/1‑150 (MQ=255)
                                                                                                                               aaGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtcttct                   <  1:3764796/150‑1 (MQ=255)
                                                                                                                               aaGCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAAtcttct                   >  1:1846505/1‑150 (MQ=255)
                                                                                                                                 gCTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTgg                 <  2:1860199/150‑1 (MQ=255)
                                                                                                                                  cTGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGt                <  1:3686634/150‑1 (MQ=255)
                                                                                                                                   tGGATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGtt               <  1:747919/150‑1 (MQ=255)
                                                                                                                                    ggATGATGATCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTg              <  1:2217941/150‑1 (MQ=255)
                                                                                                                                         atgatCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCg         <  1:1178641/150‑1 (MQ=255)
                                                                                                                                             tCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGaa     <  1:1937768/150‑1 (MQ=255)
                                                                                                                                             tCGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGaa     >  1:4216741/1‑150 (MQ=255)
                                                                                                                                              cGCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAg    >  1:4660118/1‑150 (MQ=255)
                                                                                                                                               gCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGc   <  2:3745614/150‑1 (MQ=255)
                                                                                                                                               gCATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGc   <  2:3095951/150‑1 (MQ=255)
                                                                                                                                                cATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCt  >  2:1758793/1‑150 (MQ=255)
                                                                                                                                                cATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCt  <  2:273849/150‑1 (MQ=255)
                                                                                                                                                cATAAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCt  <  2:2868094/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGGTGCAACGTGGCCCGGCGTATACCGAAATGTCGTTATTCGCGCGTATAAATGAAGACGGCAAACTGACGCTGGTGAACCATCTGGGGATTGATCTGGGCGAAACGCCGGAGCAGATCCTTAGCAAGCTGGATGATGATCGCATCAAAGATGACGATGTGCGTCACGATGGTCGTCACGCCCACGATTATGACTATGTCCACCGCGTTCGTGATATTGAAGCCGACACGCCCGCACGTTATAACGCCGATCCTGATCGGTTATTTGAATCTTCTGGTTGCGCCGGGAAGCT  >  CP009273/2216149‑2216442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑