Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,241,222 T→G 100% I264L (ATT→CTT)  lysP ← lysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,241,2220TG98.5% 1030.7 / ‑0.7 267I264L (ATT→CTT) lysPlysine transporter
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (136/127);  total (139/128)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

TACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGA  >  CP009273/2241075‑2241371
                                                                                                                                                   |                                                                                                                                                     
  cccGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCCGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATc                                                                                                                                                   <  2:4868922/150‑1 (MQ=255)
      cTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGc                                                                                                                                               >  1:1964970/1‑150 (MQ=255)
        gACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTg                                                                                                                                             <  1:2390909/150‑1 (MQ=255)
          cAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                           <  2:1137954/150‑1 (MQ=255)
          cAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                           <  2:3717878/150‑1 (MQ=255)
           aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                          >  1:2051529/1‑150 (MQ=255)
               ccgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCa                                                                                                                                      >  2:1468520/1‑150 (MQ=255)
               ccgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCa                                                                                                                                      <  2:4635630/150‑1 (MQ=255)
                cgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAg                                                                                                                                     <  1:1382187/150‑1 (MQ=255)
                cgccgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAg                                                                                                                                     >  1:2085775/1‑150 (MQ=255)
                    gTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATc                                                                                                                                 <  1:1159052/150‑1 (MQ=255)
                     tCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcg                                                                                                                                <  2:1704453/150‑1 (MQ=255)
                      cAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgc                                                                                                                               <  1:4062113/150‑1 (MQ=255)
                       aGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgcg                                                                                                                              >  2:1274615/1‑150 (MQ=255)
                        gAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGa                                                                                                                             <  1:2809306/150‑1 (MQ=255)
                         aaTAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGaa                                                                                                                            >  1:1637689/1‑150 (MQ=255)
                          aTAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAac                                                                                                                           <  1:104534/150‑1 (MQ=255)
                             aCTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAAcaca                                                                                                                        <  1:3510245/150‑1 (MQ=255)
                              cTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACAt                                                                                                                       >  1:3191037/1‑150 (MQ=255)
                               tGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATa                                                                                                                      >  1:2638829/1‑150 (MQ=255)
                               tGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATa                                                                                                                      <  1:954311/150‑1 (MQ=255)
                               tGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATa                                                                                                                      >  2:1756008/1‑150 (MQ=255)
                                   ttCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaac                                                                                                                  >  1:1544712/1‑150 (MQ=255)
                                    tcatcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaaca                                                                                                                 >  2:2839647/1‑150 (MQ=255)
                                     catcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGACGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaa                                                                                                                >  2:5067196/1‑150 (MQ=255)
                                         aCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAgg                                                                                                            >  1:4463280/1‑150 (MQ=255)
                                            gccgccgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGAtt                                                                                                         >  2:4692363/1‑150 (MQ=255)
                                            gccgccgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGAtt                                                                                                         <  1:3842772/150‑1 (MQ=255)
                                                 cgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCa                                                                                                    >  2:1543310/1‑150 (MQ=255)
                                                  gcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAg                                                                                                   <  2:4335400/150‑1 (MQ=255)
                                                     gagagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTAACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAac                                                                                                >  1:604442/1‑150 (MQ=255)
                                                      agagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAaca                                                                                               >  2:1940602/1‑150 (MQ=255)
                                                         gCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCt                                                                                            >  2:4592370/1‑150 (MQ=255)
                                                          cAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTg                                                                                           >  2:4399933/1‑150 (MQ=255)
                                                           aGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGa                                                                                          >  1:3428751/1‑150 (MQ=255)
                                                             aCCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACg                                                                                        <  2:1334150/150‑1 (MQ=255)
                                                              cccGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGt                                                                                       <  1:4175928/150‑1 (MQ=255)
                                                               ccGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                      >  1:2231539/1‑150 (MQ=255)
                                                               ccGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTa                                                                                      >  1:5078636/1‑150 (MQ=255)
                                                                  cgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTCCcg                                                                                   >  2:3039495/1‑150 (MQ=255)
                                                                   gTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgc                                                                                  <  2:782235/150‑1 (MQ=255)
                                                                     gCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGTAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcgc                                                                                <  1:773114/150‑1 (MQ=255)
                                                                      cTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgcgcg                                                                               <  2:2423034/150‑1 (MQ=255)
                                                                         gAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTgg                                                                            >  2:552941/1‑150 (MQ=255)
                                                                           acacCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGaa                                                                          <  2:4129952/150‑1 (MQ=255)
                                                                             acCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATg                                                                        <  1:1797611/150‑1 (MQ=255)
                                                                              ccAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGt                                                                       >  1:3946938/1‑150 (MQ=255)
                                                                               cAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtt                                                                      >  2:3129517/1‑150 (MQ=255)
                                                                                 gggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCTCGCGACGAATGtttt                                                                    >  2:4811021/1‑150 (MQ=255)
                                                                                  ggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttttt                                                                   >  1:4280400/1‑150 (MQ=255)
                                                                                   gTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTc                                                                  >  2:31977/1‑150 (MQ=255)
                                                                                   gTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTc                                                                  <  1:1033646/150‑1 (MQ=255)
                                                                                    tGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                                 >  2:1729681/1‑150 (MQ=255)
                                                                                    tGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                                 <  2:824342/150‑1 (MQ=255)
                                                                                    tGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCg                                                                 >  1:2952931/1‑150 (MQ=255)
                                                                                         tgACTAACGCTGATGTCTTAAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACAAAGAAAAACAGGATTCGCCATAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGa                                                            >  1:4497253/2‑150 (MQ=255)
                                                                                          gACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGAt                                                           <  1:2702600/150‑1 (MQ=255)
                                                                                            cTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                         >  1:1866312/1‑150 (MQ=255)
                                                                                            cTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                         <  1:241709/150‑1 (MQ=255)
                                                                                            cTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCt                                                         <  1:671446/150‑1 (MQ=255)
                                                                                             tAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCtt                                                        <  1:4643627/150‑1 (MQ=255)
                                                                                              aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGACTGTTTTTCGCCGGATCTTc                                                       >  2:3878432/1‑150 (MQ=255)
                                                                                               aCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCg                                                      <  2:1189023/150‑1 (MQ=255)
                                                                                                    gATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTc                                                 >  2:4950803/1‑150 (MQ=255)
                                                                                                    gATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTc                                                 <  1:4606383/150‑1 (MQ=255)
                                                                                                       gTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGcc                                              <  2:217262/150‑1 (MQ=255)
                                                                                                       gTCGTTAACATCGGTACGCAGCAGGCTCGGATCGGCGTACGGACGAATCAGGCTGATACTCCGGATCGCGCACCCAGAGCCCACCAGGAGGCGCCAGAACACCGGACGGACCGCGCGGGGAATGTTTTTCGCCGGATCTTCGGACTCGcc                                              <  2:2669184/150‑1 (MQ=255)
                                                                                                        tCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCt                                             <  2:4132969/150‑1 (MQ=255)
                                                                                                        tCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCt                                             >  1:4503914/1‑150 (MQ=255)
                                                                                                           ttAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgca                                          >  1:763846/1‑150 (MQ=255)
                                                                                                             aaCATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagc                                        >  1:4926169/1‑150 (MQ=255)
                                                                                                              aCATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagca                                       <  2:5031201/150‑1 (MQ=255)
                                                                                                               cATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCaa                                      >  2:3003644/1‑150 (MQ=255)
                                                                                                                aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                                     >  1:4840009/1‑150 (MQ=255)
                                                                                                                aTCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAAt                                     >  1:2830863/1‑150 (MQ=255)
                                                                                                                 tCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATCGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATa                                    <  1:1097581/150‑1 (MQ=255)
                                                                                                                  cGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAc                                   <  1:3082059/150‑1 (MQ=255)
                                                                                                                   gTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAcc                                  <  1:5042445/150‑1 (MQ=255)
                                                                                                                   gTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAcc                                  <  2:3401366/150‑1 (MQ=255)
                                                                                                                      aCGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGAt                               <  1:973409/150‑1 (MQ=255)
                                                                                                                      aCGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGAt                               <  2:3985691/150‑1 (MQ=255)
                                                                                                                       cGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATc                              >  2:2747578/1‑150 (MQ=255)
                                                                                                                        gcagcaGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCa                             >  1:337912/1‑150 (MQ=255)
                                                                                                                         cagcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAg                            >  2:2079706/1‑150 (MQ=255)
                                                                                                                          agcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                           >  2:1679313/1‑150 (MQ=255)
                                                                                                                          agcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                           >  2:4883549/1‑150 (MQ=255)
                                                                                                                           gcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCt                          >  1:1688329/1‑150 (MQ=255)
                                                                                                                            cagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTc                         >  2:3666545/1‑150 (MQ=255)
                                                                                                                             agGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCg                        >  1:3756316/1‑150 (MQ=255)
                                                                                                                                 tCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTc                    <  1:3812251/150‑1 (MQ=255)
                                                                                                                                  cGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTcc                   <  2:604560/150‑1 (MQ=255)
                                                                                                                                  cGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTcc                   >  1:56614/1‑150 (MQ=255)
                                                                                                                                    gATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCt                 >  1:1478586/1‑150 (MQ=255)
                                                                                                                                     aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATCGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTGTTTCGCCGGAGCTTCGGACTCGCCGGCAGCAATACCGATCAGCTCGGTTCCCTg                >  2:2661083/1‑150 (MQ=255)
                                                                                                                                         gtgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGaaa            <  1:916523/150‑1 (MQ=255)
                                                                                                                                           gtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAaga          <  2:2051529/150‑1 (MQ=255)
                                                                                                                                             aCGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga        >  1:831771/1‑150 (MQ=255)
                                                                                                                                             aCGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAgaga        >  2:1497384/1‑150 (MQ=255)
                                                                                                                                               ggAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAg      >  1:4709642/1‑150 (MQ=255)
                                                                                                                                                gaagaaTCAGGCTGATAATCAGGATCGCGAACACATATAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGc     >  1:1231452/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGA  >  CP009273/2241075‑2241371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑