BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,245,476	G→A	81.0%	A100T (GCC→ACC)	yeiI →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,245,476	0	G	A	81.0%	783.3 / 177.5	341	A100T (GCC→ACC)	yeiI	putative kinase
Reads supporting (aligned to +/- strand): ref base G (39/26); new base A (133/143); total (172/169)
Fisher's exact test for biased strand distribution p-value = 9.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 342 total aligned reads displayed.

AAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGAC  >  CP009273/2245327‑2245624

  ggTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCAcc                                                                                                                                                    >  1:972195/1‑150 (MQ=255)

   gTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGccc                                                                                                                                                   <  1:4582089/150‑1 (MQ=255)

     tACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCcac                                                                                                                                                 >  1:1688857/1‑150 (MQ=255)

       cATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACaa                                                                                                                                               <  2:306061/150‑1 (MQ=255)

         ttCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATc                                                                                                                                             >  2:4794549/1‑150 (MQ=255)

            tcACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGAGGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTgg                                                                                                                                          >  1:3681867/1‑150 (MQ=255)

              aCCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGgcg                                                                                                                                        >  1:2376041/1‑150 (MQ=255)

                cGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCt                                                                                                                                      <  2:2589003/150‑1 (MQ=255)

                cGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAAACTGGCGCt                                                                                                                                      >  1:4766755/1‑150 (MQ=255)

                cGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCt                                                                                                                                      >  1:2931738/1‑150 (MQ=255)

                cGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCt                                                                                                                                      >  2:1911280/1‑150 (MQ=255)

                cGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCt                                                                                                                                      >  2:3755768/1‑150 (MQ=255)

                cGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCt                                                                                                                                      >  2:862377/1‑150 (MQ=255)

                 gAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTg                                                                                                                                     >  2:3551880/1‑150 (MQ=255)

                  aGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGt                                                                                                                                    >  2:939864/1‑150 (MQ=255)

                  aGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGt                                                                                                                                    >  2:1034475/1‑150 (MQ=255)

                     aGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAg                                                                                                                                 >  1:697342/1‑150 (MQ=255)

                      ggAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAgg                                                                                                                                <  2:4483762/150‑1 (MQ=255)

                       gAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGc                                                                                                                               >  1:1916980/1‑150 (MQ=255)

                         aTACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCg                                                                                                                             >  2:2701078/1‑150 (MQ=255)

                           aCTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTg                                                                                                                           >  2:2822429/1‑150 (MQ=255)

                             tGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGAc                                                                                                                         <  2:4064979/150‑1 (MQ=255)

                             tGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGAc                                                                                                                         >  1:252940/1‑150 (MQ=255)

                                gTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTc                                                                                                                      <  1:2339123/150‑1 (MQ=255)

                                   gtggtgGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCAt                                                                                                                   <  1:526458/150‑1 (MQ=255)

                                      gtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTg                                                                                                                <  1:4158826/150‑1 (MQ=255)

                                      gtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTg                                                                                                                <  1:1018145/150‑1 (MQ=255)

                                       tggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGc                                                                                                               <  1:2564010/150‑1 (MQ=255)

                                            aCAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCa                                                                                                          >  1:2844954/1‑150 (MQ=255)

                                               atcaatATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTg                                                                                                       <  1:4724934/150‑1 (MQ=255)

                                                   atatGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTg                                                                                                   <  1:1297352/150‑1 (MQ=255)

                                                   atatGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTg                                                                                                   <  1:1167998/150‑1 (MQ=255)

                                                   atatGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTg                                                                                                   >  1:3885483/1‑150 (MQ=255)

                                                      tGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCg                                                                                                >  2:2118223/1‑150 (MQ=255)

                                                       ggATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGa                                                                                               >  1:1073730/1‑150 (MQ=255)

                                                        gATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGAt                                                                                              <  1:2791711/150‑1 (MQ=255)

                                                          tatTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGa                                                                                            <  1:1892521/150‑1 (MQ=255)

                                                           atTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGAAGTCCATTTGCTTTCAGTGATTGGCGATGAc                                                                                           >  1:3968940/1‑150 (MQ=255)

                                                           atTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGAAGTCCATTTGCTTTCAGTGATTGGCGATGAc                                                                                           >  1:1390384/1‑150 (MQ=255)

                                                              cgcgGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACttt                                                                                        >  1:595164/1‑150 (MQ=255)

                                                                 gggATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCGACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTAt                                                                                     <  2:2223885/150‑1 (MQ=255)

                                                                  ggATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATg                                                                                    >  1:1805453/1‑150 (MQ=255)

                                                                   taTGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATgg                                                                                   >  2:496688/2‑150 (MQ=255)

                                                                   gATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATgg                                                                                   >  1:1225746/1‑150 (MQ=255)

                                                                     tGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCg                                                                                 <  2:4990349/150‑1 (MQ=255)

                                                                      ggCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGa                                                                                <  2:3940511/150‑1 (MQ=255)

                                                                      ggCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGa                                                                                <  1:711808/150‑1 (MQ=255)

                                                                       gCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGaa                                                                               <  2:1705253/150‑1 (MQ=255)

                                                                        cGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGaaa                                                                              <  1:266046/150‑1 (MQ=255)

                                                                         ggATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAAt                                                                             <  2:4432918/150‑1 (MQ=255)

                                                                          gATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATg                                                                            >  2:3103908/1‑150 (MQ=255)

                                                                           atatCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGc                                                                           >  2:2890775/1‑150 (MQ=255)

                                                                           atatCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGc                                                                           <  2:4702327/150‑1 (MQ=255)

                                                                             atCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTc                                                                         >  2:3615045/1‑150 (MQ=255)

                                                                              tCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTcc                                                                        <  2:1139888/150‑1 (MQ=255)

                                                                                cGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTg                                                                      <  2:5088043/150‑1 (MQ=255)

                                                                                  ttACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGga                                                                    >  1:671015/1‑150 (MQ=255)

                                                                                     cccGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGgaaga                                                                 >  2:2439283/1‑150 (MQ=255)

                                                                                       cGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGaaa                                                               >  1:5007460/1‑150 (MQ=255)

                                                                                          aaTCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAAcgc                                                            <  1:4216195/150‑1 (MQ=255)

                                                                                           aTCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAAcgcg                                                           >  1:2055584/1‑150 (MQ=255)

                                                                                             cGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGcc                                                         <  2:767878/150‑1 (MQ=255)

                                                                                             cGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGcc                                                         <  2:4334067/150‑1 (MQ=255)

                                                                                             cGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGcc                                                         >  2:2306513/1‑150 (MQ=255)

                                                                                              ggCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCg                                                        >  1:936129/1‑150 (MQ=255)

                                                                                               gCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGt                                                       <  1:1267042/150‑1 (MQ=255)

                                                                                               gCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGt                                                       <  2:4009768/150‑1 (MQ=255)

                                                                                                  tctcATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACTTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGcc                                                    <  1:2183150/150‑1 (MQ=255)

                                                                                                    tcATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCgg                                                  >  2:347084/1‑150 (MQ=255)

                                                                                                      aTCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCg                                                >  2:5152767/1‑150 (MQ=255)

                                                                                                       tCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGt                                               <  2:571319/150‑1 (MQ=255)

                                                                                                          cGGTACAATACATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGaa                                            >  2:2162722/1‑150 (MQ=255)

                                                                                                           ggTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAAt                                           >  2:1570626/1‑150 (MQ=255)

                                                                                                             tACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGt                                         >  2:3741354/1‑150 (MQ=255)

                                                                                                               cAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGtct                                       <  1:1245955/150‑1 (MQ=255)

                                                                                                               cAATTCATTGCTCAGCGGGCGGCGTGGGACGAAACATCACCCACTATCTGGCGATGTTAGGCCGTGAAGTCCATTTGCTTTCTGTGATGGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGtct                                       <  1:2271239/150‑1 (MQ=255)

                                                                                                                 aTTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACTCGCCGTGCCGGCGTGAATGTCTcc                                     >  2:2986751/1‑150 (MQ=255)

                                                                                                                 aTTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTcc                                     <  2:329230/150‑1 (MQ=255)

                                                                                                                 aTTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTcc                                     <  2:1385900/150‑1 (MQ=255)

                                                                                                                   tCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCgg                                   <  2:2958880/150‑1 (MQ=255)

                                                                                                                   tCATTGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCgg                                   >  2:3698779/1‑150 (MQ=255)

                                                                                                                      ttGCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTg                                >  2:4183139/1‑150 (MQ=255)

                                                                                                                        gCTCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTCATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGca                              <  1:3206325/150‑2 (MQ=255)

                                                                                                                          tCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgtt                            >  2:3680698/1‑150 (MQ=255)

                                                                                                                          tCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgtt                            >  1:3314543/1‑150 (MQ=255)

                                                                                                                          tCAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgtt                            >  1:3701164/1‑150 (MQ=255)

                                                                                                                           cAGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgttc                           <  2:722293/150‑1 (MQ=255)

                                                                                                                            aGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgttcg                          <  1:1511823/150‑1 (MQ=255)

                                                                                                                            aGCGGGCGGCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgttcg                          <  2:1852124/150‑1 (MQ=255)

                                                                                                                               gggcggCGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGttt                       >  1:1435249/1‑150 (MQ=255)

                                                                                                                                ggcggcGTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTg                      <  1:406266/150‑1 (MQ=255)

                                                                                                                                  cggcgTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCa                    <  2:2491055/150‑1 (MQ=255)

                                                                                                                                  cggcgTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCa                    >  2:1271036/1‑150 (MQ=255)

                                                                                                                                   ggcgTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCAt                   >  2:426247/1‑150 (MQ=255)

                                                                                                                                    gcgTGGGACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATg                  <  1:4922578/150‑1 (MQ=255)

                                                                                                                                       tGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTc               >  1:292418/1‑150 (MQ=255)

                                                                                                                                       tGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGGTCGTTTGCATGGTc               >  1:3433554/1‑150 (MQ=255)

                                                                                                                                        gggACGCAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCa              <  2:1404719/150‑1 (MQ=255)

                                                                                                                                              cAACATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGcac        >  2:3673238/1‑150 (MQ=255)

                                                                                                                                                 cATCACCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATc     <  2:1781492/150‑1 (MQ=255)

AAGGTTACATTCTCACCGAGCAGGAATACTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGAC  >  CP009273/2245327‑2245624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`