Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,352,573 C→A 100% P353P (CCG→CCT)  rhmT ← putative L‑rhamnonate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,352,5730CA99.7% 1029.8 / ‑4.8 311P353P (CCG→CCT) rhmTputative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (153/156);  total (154/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 311 total aligned reads displayed.

TGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCG  >  CP009273/2352426‑2352719
                                                                                                                                                   |                                                                                                                                                  
tGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACACAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAgg                                                                                                                                                  >  2:4782100/1‑150 (MQ=255)
tGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAgg                                                                                                                                                  <  2:2865441/150‑1 (MQ=255)
tGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAAGTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAgg                                                                                                                                                  <  2:1218401/150‑1 (MQ=255)
    aGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATc                                                                                                                                              <  1:3524921/150‑1 (MQ=255)
    aGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATc                                                                                                                                              >  2:4560144/1‑150 (MQ=255)
         cAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCa                                                                                                                                         >  2:1993298/1‑150 (MQ=255)
          aGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAg                                                                                                                                        <  2:697693/150‑1 (MQ=255)
          aGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAg                                                                                                                                        >  1:476139/1‑150 (MQ=255)
                cccGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACt                                                                                                                                  <  2:2909095/150‑1 (MQ=255)
                 ccgccgCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTg                                                                                                                                 >  1:3026565/1‑150 (MQ=255)
                      gcATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGc                                                                                                                            >  1:3545467/1‑150 (MQ=255)
                       cATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCa                                                                                                                           <  2:4274544/150‑1 (MQ=255)
                       cATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCa                                                                                                                           >  2:758878/1‑150 (MQ=255)
                        aTCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAAGTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCAc                                                                                                                          <  1:3736253/150‑1 (MQ=255)
                         tCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACg                                                                                                                         <  1:1047617/150‑1 (MQ=255)
                          cGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACgg                                                                                                                        >  1:4597302/1‑150 (MQ=255)
                           gCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGc                                                                                                                       <  2:4668269/150‑1 (MQ=255)
                            cTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCa                                                                                                                      <  1:3253250/150‑1 (MQ=255)
                             tGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCaa                                                                                                                     >  1:2049346/1‑150 (MQ=255)
                             tGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCaa                                                                                                                     >  1:722708/1‑150 (MQ=255)
                              ggCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAAt                                                                                                                    <  1:2146246/150‑1 (MQ=255)
                                cAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaa                                                                                                                  >  1:990853/1‑150 (MQ=255)
                                cAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATaa                                                                                                                  <  1:2770270/150‑1 (MQ=255)
                                 aaaTAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTATGCATCGTCCAGAACACTGGCTGCACGGCAATaaa                                                                                                                 >  1:1898769/1‑150 (MQ=255)
                                    tAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAAccc                                                                                                              <  1:1335384/150‑1 (MQ=255)
                                     aaCGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCg                                                                                                             <  2:2353/150‑1 (MQ=255)
                                     aaCGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCg                                                                                                             >  2:4865818/1‑150 (MQ=255)
                                     aaCGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCg                                                                                                             <  2:2382/150‑1 (MQ=255)
                                       cGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGAt                                                                                                           <  2:2647229/150‑1 (MQ=255)
                                        gTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                          <  1:1725584/150‑1 (MQ=255)
                                        gTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATa                                                                                                          >  2:1675920/1‑150 (MQ=255)
                                            cTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTg                                                                                                      <  1:1792799/150‑1 (MQ=255)
                                                 ttCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAaca                                                                                                 >  2:1546993/1‑150 (MQ=255)
                                                  tCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacac                                                                                                <  2:4053854/150‑1 (MQ=255)
                                                   cACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacaca                                                                                               <  1:4199636/150‑1 (MQ=255)
                                                    aCGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacacac                                                                                              <  2:601823/150‑1 (MQ=255)
                                                    aCGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAacacac                                                                                              <  2:2137180/150‑1 (MQ=255)
                                                     cgcgCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAAcacaca                                                                                             <  2:3095600/150‑1 (MQ=255)
                                                        gcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcg                                                                                          >  1:971683/1‑150 (MQ=255)
                                                        gcAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcg                                                                                          <  1:2113714/150‑1 (MQ=255)
                                                         cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACATGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcgc                                                                                         >  1:3306140/1‑150 (MQ=255)
                                                         cAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAgcgc                                                                                         >  1:4440018/1‑150 (MQ=255)
                                                          aGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCt                                                                                        >  1:5146922/1‑150 (MQ=255)
                                                          aGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCt                                                                                        >  1:2274103/1‑150 (MQ=255)
                                                          aGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCt                                                                                        >  1:2267684/1‑150 (MQ=255)
                                                           ggATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTa                                                                                       <  1:1487895/150‑1 (MQ=255)
                                                             aTCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg                                                                                     <  2:3153203/150‑1 (MQ=255)
                                                             aTCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACg                                                                                     <  2:269120/150‑1 (MQ=255)
                                                               ctgggCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                   >  1:3981141/3‑150 (MQ=255)
                                                               cGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGAt                                                                                   >  2:4096247/1‑150 (MQ=255)
                                                                    cAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCa                                                                              <  2:1480405/150‑1 (MQ=255)
                                                                    cAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCa                                                                              >  2:5064742/1‑150 (MQ=255)
                                                                    cAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCa                                                                              >  1:5015825/1‑150 (MQ=255)
                                                                        aaaCCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGACAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTAc                                                                          >  2:1761621/1‑150 (MQ=255)
                                                                            ccGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGga                                                                      >  2:4303624/1‑150 (MQ=255)
                                                                             cGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGgag                                                                     >  1:3629252/1‑150 (MQ=255)
                                                                                  aCTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAagc                                                                <  1:4452044/150‑1 (MQ=255)
                                                                                  aCTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAagc                                                                <  2:3901404/150‑1 (MQ=255)
                                                                                   cTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAagca                                                               <  2:1081148/150‑1 (MQ=255)
                                                                                        ccAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGGTAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCg                                                          >  1:1161436/1‑150 (MQ=255)
                                                                                        ccAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCg                                                          >  2:491648/1‑150 (MQ=255)
                                                                                        ccAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCg                                                          <  1:2687889/150‑1 (MQ=255)
                                                                                        ccAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCg                                                          >  1:3128302/1‑150 (MQ=255)
                                                                                         cAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCgg                                                         <  1:2400106/150‑1 (MQ=255)
                                                                                          aaaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCCTTACTGGAGAAAGCAGCCCGGa                                                        >  2:4209713/1‑150 (MQ=255)
                                                                                           aaCAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGAc                                                       >  1:3356220/1‑150 (MQ=255)
                                                                                                gTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGacc                                                  <  2:1973193/150‑1 (MQ=255)
                                                                                                gTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGacc                                                  <  2:5154043/150‑1 (MQ=255)
                                                                                                     cAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatac                                             <  1:3927206/150‑1 (MQ=255)
                                                                                                      aaaaCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaatacc                                            <  2:953983/150‑1 (MQ=255)
                                                                                                         aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                         <  2:1888388/150‑1 (MQ=255)
                                                                                                         aCCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGaccaataccaat                                         <  2:3260402/150‑1 (MQ=255)
                                                                                                           cAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGc                                       <  2:1125212/150‑1 (MQ=255)
                                                                                                            aaTTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGcc                                      >  1:1972109/1‑150 (MQ=255)
                                                                                                                cccGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgcc                                  >  1:1543827/1‑150 (MQ=255)
                                                                                                                 ccGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCccgccg                                 >  1:4808/1‑150 (MQ=255)
                                                                                                                  cGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCcgccgc                                >  1:85091/1‑150 (MQ=255)
                                                                                                                     agccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                             >  2:4405054/1‑150 (MQ=255)
                                                                                                                     agccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                             >  2:711835/1‑150 (MQ=255)
                                                                                                                     agccagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAg                             >  1:3341933/1‑150 (MQ=255)
                                                                                                                        cagcGCCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTaa                          >  1:892125/1‑150 (MQ=255)
                                                                                                                           cgcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGt                       <  1:4895096/150‑1 (MQ=255)
                                                                                                                            gcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTc                      >  1:2798569/1‑150 (MQ=255)
                                                                                                                            gcCGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTc                      >  2:3827527/1‑150 (MQ=255)
                                                                                                                              cGTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAg                    <  1:4037836/150‑1 (MQ=255)
                                                                                                                               gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAgc                   <  1:1593698/150‑1 (MQ=255)
                                                                                                                               gTACCGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAgc                   >  2:2271472/1‑150 (MQ=255)
                                                                                                                                  ccGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCt                <  2:2181249/150‑1 (MQ=255)
                                                                                                                                  ccGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCt                >  1:1627417/1‑150 (MQ=255)
                                                                                                                                   cGGAAAGCAGTTGTGTAGGCATCGTCCAGAACCCTGGCTGCACGGCAAGAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCCGTACTGGAGCACGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTACTGTCAGCGCTg               >  2:350534/1‑150 (MQ=255)
                                                                                                                                   cGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTg               <  1:1546963/150‑1 (MQ=255)
                                                                                                                                   cGGAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTg               >  1:2662620/1‑150 (MQ=255)
                                                                                                                                    ggAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGc              >  2:3373561/1‑150 (MQ=255)
                                                                                                                                    ggAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGc              >  2:5127792/1‑150 (MQ=255)
                                                                                                                                     gAAAGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCg             <  2:3977726/150‑1 (MQ=255)
                                                                                                                                      aaaGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGa            >  2:1281865/1‑150 (MQ=255)
                                                                                                                                      aaaGCAGTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGa            >  1:1668206/1‑150 (MQ=255)
                                                                                                                                            gTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTAc      <  2:3300851/150‑1 (MQ=255)
                                                                                                                                            gTTGTGTAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTAc      <  1:1437915/150‑1 (MQ=255)
                                                                                                                                               gtgtAGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGcc   <  2:3839067/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCCAGCGTCAGTAATCCCGCCGCATCGCTGGCAAATAACGTTTCTGCTTTCACGCGCAGGATCGGGGCAATAAACCCGCCCACTGCACCAAACAGGTTTACAAAACCAATTCCCGCAGCCAGCGCCGTACCGGAAAGCAGTTGTGTCGGCATCGTCCAGAACACTGGCTGCACGGCAATAAACCCGATAGCTGCAACACACAGCGCTACGATCGCCATTACTGGAGAAAGCAGCCCGGACAGACCAATACCAATGCCCGCCGCCAGTAATGTCAGCGCTGCGACATTACGCCG  >  CP009273/2352426‑2352719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑