Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,380,078 C→T 100% S247F (TCT→TTT)  yfbL → putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,380,0780CT98.5% 881.5 / 1.2 266S247F (TCT→TTT) yfbLputative peptidase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (123/139);  total (125/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

GCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGC  >  CP009273/2379931‑2380224
                                                                                                                                                   |                                                                                                                                                  
 cTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCa                                                                                                                                                 <  2:2464180/150‑1 (MQ=255)
  tATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAg                                                                                                                                                >  2:2803500/1‑150 (MQ=255)
    ttACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGa                                                                                                                                              <  1:1975053/150‑1 (MQ=255)
     tACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAt                                                                                                                                             <  1:4903634/150‑1 (MQ=255)
      aCGACTCTGCGCCTGGAAGCCAGAATTACCCTTCCCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGAtt                                                                                                                                            <  1:2879900/150‑1 (MQ=255)
      aCGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATCGTTGTCATTTCAGGAtt                                                                                                                                            <  1:286155/150‑1 (MQ=255)
         aCTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTAt                                                                                                                                         <  2:1586625/150‑1 (MQ=255)
          ctctGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATc                                                                                                                                        >  2:1377491/1‑150 (MQ=255)
          ctctGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATc                                                                                                                                        >  1:4762349/1‑150 (MQ=255)
           tctGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCt                                                                                                                                       <  1:4397605/150‑1 (MQ=255)
              gcgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGtt                                                                                                                                    <  1:4480603/150‑1 (MQ=255)
              gcgcCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGtt                                                                                                                                    <  2:3673884/150‑1 (MQ=255)
                 ccTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTAt                                                                                                                                 >  2:4687740/1‑150 (MQ=255)
                   tGGAAGCCAGAATTACCCTTCCCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTc                                                                                                                               <  2:443111/150‑1 (MQ=255)
                      aaGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTAt                                                                                                                            >  1:888699/1‑150 (MQ=255)
                             aaTTACCCGTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcc                                                                                                                     <  2:2924577/150‑1 (MQ=255)
                              aTTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAccc                                                                                                                    >  2:4852543/1‑150 (MQ=255)
                               ttacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATAcccc                                                                                                                   <  1:4248707/150‑1 (MQ=255)
                                tacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCa                                                                                                                  >  2:4165064/1‑150 (MQ=255)
                                tacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCa                                                                                                                  >  1:3431294/1‑150 (MQ=255)
                                tacccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCa                                                                                                                  <  2:3217392/150‑1 (MQ=255)
                                 acccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAg                                                                                                                 >  2:1070094/1‑150 (MQ=255)
                                  cccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAgg                                                                                                                <  2:4276359/150‑1 (MQ=255)
                                  cccttacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAgg                                                                                                                <  2:3544615/150‑1 (MQ=255)
                                      tacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGttt                                                                                                            <  2:117063/150‑1 (MQ=255)
                                      tacccGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGttt                                                                                                            <  1:3174864/150‑1 (MQ=255)
                                            gCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTccc                                                                                                      <  1:2169609/150‑1 (MQ=255)
                                              aaTGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGCATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCgg                                                                                                    <  2:5151133/150‑1 (MQ=255)
                                                tGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTa                                                                                                  >  1:2739877/1‑150 (MQ=255)
                                                   ccTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTg                                                                                               <  1:370177/150‑1 (MQ=255)
                                                     tGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAt                                                                                             >  2:4953707/1‑150 (MQ=255)
                                                     tGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAt                                                                                             >  1:4451458/1‑150 (MQ=255)
                                                     tGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAt                                                                                             >  1:1880587/1‑150 (MQ=255)
                                                      ggCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGAtt                                                                                            >  1:1963556/1‑150 (MQ=255)
                                                        cTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtc                                                                                          <  2:3308961/150‑1 (MQ=255)
                                                          ttATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtctc                                                                                        >  2:4828843/1‑150 (MQ=255)
                                                          ttATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTtctc                                                                                        >  2:2822056/1‑150 (MQ=255)
                                                           tATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTctct                                                                                       <  1:2276523/150‑1 (MQ=255)
                                                           tATCCCGATCGGGGAGACTTTAGTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTctct                                                                                       <  2:3946159/150‑1 (MQ=255)
                                                               ccGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGacc                                                                                   <  1:1654875/150‑1 (MQ=255)
                                                                cGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGacca                                                                                  <  2:34480/150‑1 (MQ=255)
                                                                cGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGacca                                                                                  <  2:1818737/150‑1 (MQ=255)
                                                                 gATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGaccac                                                                                 >  2:501778/1‑150 (MQ=255)
                                                                    cGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTg                                                                              <  1:1804460/150‑1 (MQ=255)
                                                                       ggAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAat                                                                           <  1:1014373/150‑1 (MQ=255)
                                                                       ggAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAat                                                                           <  2:2859678/150‑1 (MQ=255)
                                                                       ggAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAat                                                                           >  1:5044740/1‑150 (MQ=255)
                                                                        gagaCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAatt                                                                          <  1:570114/150‑1 (MQ=255)
                                                                           aCTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAattatt                                                                       <  1:3089715/150‑1 (MQ=255)
                                                                            cTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTg                                                                      <  1:1377491/150‑1 (MQ=255)
                                                                            cTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTg                                                                      >  1:3460433/1‑150 (MQ=255)
                                                                               tATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCa                                                                   <  1:2530086/150‑1 (MQ=255)
                                                                                aTTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCaa                                                                  >  2:4128695/1‑150 (MQ=255)
                                                                                  tGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAaca                                                                <  1:2105855/150‑1 (MQ=255)
                                                                                   gCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAacac                                                               >  1:5138833/1‑150 (MQ=255)
                                                                                    ccGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACg                                                              <  1:4166005/150‑1 (MQ=255)
                                                                                     cGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGa                                                             >  2:1252041/1‑150 (MQ=255)
                                                                                      gTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGat                                                            <  1:2666705/150‑1 (MQ=255)
                                                                                       tGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGata                                                           <  1:2396284/150‑1 (MQ=255)
                                                                                        ggTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGatat                                                          >  2:198275/1‑150 (MQ=255)
                                                                                            ggCAGAATACAGGATATCAACGCCGTTCGTCGGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTccc                                                      >  1:3282742/1‑150 (MQ=255)
                                                                                               aGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGcc                                                   <  1:3192682/150‑1 (MQ=255)
                                                                                                gAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCa                                                  <  1:388570/150‑1 (MQ=255)
                                                                                                 aaTACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCAt                                                 >  1:2253866/1‑150 (MQ=255)
                                                                                                  aTACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATa                                                <  2:4566193/150‑1 (MQ=255)
                                                                                                  aTACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATa                                                <  2:2580568/150‑1 (MQ=255)
                                                                                                     cAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATg                                             >  2:4339969/1‑150 (MQ=255)
                                                                                                     cAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATg                                             <  1:803976/150‑1 (MQ=255)
                                                                                                      aGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGa                                            <  1:3566683/150‑1 (MQ=255)
                                                                                                       ggATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGAt                                           >  2:255209/1‑150 (MQ=255)
                                                                                                           atCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACt                                       <  1:622825/150‑1 (MQ=255)
                                                                                                           atCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACt                                       >  2:307370/1‑150 (MQ=255)
                                                                                                           atCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACt                                       >  2:1498586/1‑150 (MQ=255)
                                                                                                              aaCGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGac                                    <  1:1513090/150‑1 (MQ=255)
                                                                                                               aCGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGaca                                   <  2:4762349/150‑1 (MQ=255)
                                                                                                                cGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGacac                                  <  1:2700858/150‑1 (MQ=255)
                                                                                                                 gCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACAcc                                 >  2:3100266/1‑150 (MQ=255)
                                                                                                                 gCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACAcc                                 >  2:3728251/1‑150 (MQ=255)
                                                                                                                    gTTCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCt                              >  2:2077499/1‑150 (MQ=255)
                                                                                                                     ttCGTCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCtt                             <  2:2269648/150‑1 (MQ=255)
                                                                                                                        gTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCttttt                          <  1:4908554/150‑1 (MQ=255)
                                                                                                                         tCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTa                         >  1:1996946/1‑150 (MQ=255)
                                                                                                                         tCAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTa                         <  2:596959/150‑1 (MQ=255)
                                                                                                                          cAGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTAt                        >  2:1231710/1‑150 (MQ=255)
                                                                                                                           aGGTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATc                       <  1:1293682/150‑1 (MQ=255)
                                                                                                                            ggTAAAAGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCg                      <  2:2258256/150‑1 (MQ=255)
                                                                                                                               aaaaGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaa                   <  2:1349666/150‑1 (MQ=255)
                                                                                                                                aaaGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaat                  <  1:3011488/150‑1 (MQ=255)
                                                                                                                                aaaGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaat                  >  2:2598922/1‑150 (MQ=255)
                                                                                                                                 aaGCGGCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGtaata                 <  2:306380/150‑1 (MQ=255)
                                                                                                                                      gCATTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACaa            >  2:3475469/1‑150 (MQ=255)
                                                                                                                                        aTTGTTGTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATa          <  2:1359976/150‑1 (MQ=255)
                                                                                                                                         ttgttgTCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATAc         >  2:863920/1‑150 (MQ=255)
                                                                                                                                           gttgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCa       <  1:4025248/150‑1 (MQ=255)
                                                                                                                                           gttgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCa       <  1:1070094/150‑1 (MQ=255)
                                                                                                                                             tgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACt     >  2:2234478/1‑150 (MQ=255)
                                                                                                                                             tgtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACt     >  2:2063938/1‑150 (MQ=255)
                                                                                                                                              gtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACACTACCACtt    <  2:320474/150‑1 (MQ=255)
                                                                                                                                              gtCATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACtt    >  2:4221231/1‑150 (MQ=255)
                                                                                                                                                cATTTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGc  >  1:1686671/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTATTACGACTCTGCGCCTGGAAGCCAGAATTACCCTTACCCGGCAATGTCCTGGCTTTATCCCGATCGGGGAGACTTTATTGCCGTGGTCGGCAGAATACAGGATATCAACGCCGTTCGTCAGGTAAAAGCGGCATTGTTGTCATCTCAGGATTTATCTGTTTATTCTATGAATACCCCAGGGTTTATTCCCGGTATTGATTTCTCTGACCACCTGAATTATTGGCAACACGATATTCCCGCCATAATGATTACTGACACCGCTTTTTATCGTAATAAACAATACCACTTGC  >  CP009273/2379931‑2380224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑