Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,404,772 C→A 100% A20A (GCG→GCT)  yfbS ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,404,7720CA98.8% 1079.6 / 2.2 332A20A (GCG→GCT) yfbSputative transporter
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base A (191/136);  total (194/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 332 total aligned reads displayed.

ACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404625‑2404918
                                                                                                                                                   |                                                                                                                                                  
   aTCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCaaa                                                                                                                                               >  1:498032/1‑150 (MQ=255)
      gCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACaa                                                                                                                                            >  1:2822403/1‑150 (MQ=255)
        cAATACTAACCACGGCGGCAATCCGTACAACGATAGGATCATAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTAAATGAAAACGCTAAAATAACAACCACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGa                                                                                                                                          <  2:375725/150‑1 (MQ=255)
           taataaACAAGGCGGCAATCAGGACAACTTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGa                                                                                                                                       <  2:2478509/150‑1 (MQ=255)
             ataaACACGGCGTAAACCCGGACAACTTTTGTAAAATAAACGCCTTAAAATACTTCTGGTATTGACCGCTTTCCGCTAAATCAAAACGCTACAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATg                                                                                                                                     <  1:943199/150‑1 (MQ=255)
               aaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGc                                                                                                                                   >  1:1359414/1‑150 (MQ=255)
                   aaGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACg                                                                                                                               >  2:3043624/1‑150 (MQ=255)
                    aGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACgg                                                                                                                              >  2:4930051/1‑150 (MQ=255)
                       cggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCa                                                                                                                           >  2:693876/1‑150 (MQ=255)
                              cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGa                                                                                                                    >  1:305437/1‑150 (MQ=255)
                                 gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                                 >  2:1048398/1‑150 (MQ=255)
                                 gACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAg                                                                                                                 >  1:3193521/1‑150 (MQ=255)
                                  aCAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGa                                                                                                                >  1:1198374/1‑150 (MQ=255)
                                  aCAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGa                                                                                                                >  1:3719023/1‑150 (MQ=255)
                                   cAACTTTAGGATCATAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGATTAATGAAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGaa                                                                                                               <  1:2986835/150‑1 (MQ=255)
                                      cGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAccc                                                                                                            >  1:2526510/1‑150 (MQ=255)
                                         tAGTATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                         <  2:1696544/150‑1 (MQ=255)
                                         tAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                         >  1:2432416/1‑150 (MQ=255)
                                         tAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAAAGCGCCAATAACAAACAAATCGACCGCATCCATACGAACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaaa                                                                                                         >  1:1971108/1‑150 (MQ=255)
                                          aGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                                                                        <  2:952702/150‑1 (MQ=255)
                                           ggATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATc                                                                                                       <  2:3408193/150‑1 (MQ=255)
                                            gATCATAAAAGCCGTAAAATACTTCTGGGACTGTCTGCGTTCCGCTTAATGAAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                      <  2:1234662/150‑1 (MQ=255)
                                            gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                      >  1:1222661/1‑150 (MQ=255)
                                            gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                                                      >  2:2119520/1‑150 (MQ=255)
                                             aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                     >  2:2254184/1‑150 (MQ=255)
                                             aTCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCaa                                                                                                     >  2:4170/1‑150 (MQ=255)
                                              taataaaaGCCGTAAAATACTTCTGGTACTGTCAGCGTTCCGATTAATCCAAACGCTACAATAAAAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                                                                    <  1:3259312/146‑1 (MQ=255)
                                              tCATAAAAGCCGTAAATTACTTCTGGTACTGTCAGCTTTCCGCTTAATGCAAACGCTAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                                                                    <  1:2568006/150‑1 (MQ=255)
                                               cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                                                   <  2:1596107/150‑1 (MQ=255)
                                                   aaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                                               >  1:3565528/1‑150 (MQ=255)
                                                    aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                              >  1:3235891/1‑150 (MQ=255)
                                                    aaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCg                                                                                              >  2:2818404/1‑150 (MQ=255)
                                                     agccttaaaaTACTTCGGGGAGTGTCTGCTTTCCTTTTAATGAAACCGCGAAAATAAAAAACACAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                                             <  1:511044/144‑1 (MQ=255)
                                                     aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                                             >  2:1346524/1‑150 (MQ=255)
                                                     aGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGt                                                                                             >  2:515285/1‑150 (MQ=255)
                                                      gCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGtt                                                                                            >  1:541773/1‑150 (MQ=255)
                                                       cgttaaaaTTCTTGTGGGAGTGTCTGCTTTCCGATTATTCCAACCGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                                                           <  1:4505582/146‑1 (MQ=255)
                                                       ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                                                           >  2:4071633/1‑150 (MQ=255)
                                                       ccGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTc                                                                                           >  2:4668261/1‑150 (MQ=255)
                                                        cGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                                                                          >  2:918317/1‑150 (MQ=255)
                                                        cGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                                                                          >  1:2621313/1‑150 (MQ=255)
                                                             aaTACTTCTGGGACTGTCAGCGTTCCGATTAATGAAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACt                                                                                     <  2:3666951/150‑1 (MQ=255)
                                                              aTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACtt                                                                                    >  2:2373082/1‑150 (MQ=255)
                                                              aTACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACtt                                                                                    >  2:2672037/1‑150 (MQ=255)
                                                               tACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTa                                                                                   >  1:1527770/1‑150 (MQ=255)
                                                                      gggACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                                                                            >  1:2954111/1‑150 (MQ=255)
                                                                          cTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAt                                                                        <  2:4437862/150‑1 (MQ=255)
                                                                           tGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAtt                                                                       >  2:5018129/1‑150 (MQ=255)
                                                                            gtctgggTTCCGATTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                      <  1:540784/144‑1 (MQ=255)
                                                                            gTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGACCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTg                                                                      >  2:2845652/1‑150 (MQ=255)
                                                                                  gTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggggg                                                                <  1:1694964/150‑1 (MQ=255)
                                                                                    tCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGAAACGGCCAGTAATGAAAGAACCCAACTCAATTCACCGTTGACAACTTATCCGTGTTACGTGAGGGGGAt                                                              >  2:2633418/1‑150 (MQ=255)
                                                                                     ccGCTTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATg                                                             <  2:1373757/150‑1 (MQ=255)
                                                                                     ccGATTAATGAAAACGCCAAAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATg                                                             <  1:4086188/150‑1 (MQ=255)
                                                                                       gCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGAc                                                           >  1:3806978/1‑150 (MQ=255)
                                                                                          tAATGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTg                                                        <  1:4189685/150‑1 (MQ=255)
                                                                                           aagccAAACGCCACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                                                       <  1:5005375/146‑1 (MQ=255)
                                                                                             tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                                                     >  2:4294462/1‑150 (MQ=255)
                                                                                              gCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTc                                                    >  1:2657686/1‑150 (MQ=255)
                                                                                                aaaCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTg                                                  >  1:824848/1‑150 (MQ=255)
                                                                                                 aaCGCGACAATAACAAACAAAGCGACCGAATCAATACTCACTCTGCCAGTAGCAAACAAGACTATGTCAACGGCCAGTAATGAAAGAACACAAATCAAATCACCGTTCAAAACTTATCCTTGTTAATTGAGGGGGATTACTTGATTATGc                                                 >  1:4387968/1‑150 (MQ=255)
                                                                                                       aCAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaa                                           <  2:5110004/150‑1 (MQ=255)
                                                                                                        cAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaa                                          >  1:4928043/1‑150 (MQ=255)
                                                                                                         aaTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                         <  2:3914197/150‑1 (MQ=255)
                                                                                                         aaTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa                                         >  1:1088032/1‑150 (MQ=255)
                                                                                                           tAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAg                                       <  2:4120522/150‑1 (MQ=255)
                                                                                                             aaaaaaaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc                                     <  2:3472200/148‑1 (MQ=255)
                                                                                                              caaacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                    <  1:3351846/150‑1 (MQ=255)
                                                                                                              caaacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGccc                                    >  1:1997956/1‑150 (MQ=255)
                                                                                                                aacaaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcg                                  >  2:1561429/1‑150 (MQ=255)
                                                                                                                  caaaGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac                                >  2:741223/1‑150 (MQ=255)
                                                                                                                     aGCGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgag                             >  1:4250018/1‑150 (MQ=255)
                                                                                                                      gCGACCGCATACATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATATATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACgaga                            <  2:291947/150‑1 (MQ=255)
                                                                                                                       cGACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAACTCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGAc                           <  2:1753602/150‑1 (MQ=255)
                                                                                                                        gACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGTCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGACATAAAAAAGACCCGACGAGACg                          >  1:1006837/1‑150 (MQ=255)
                                                                                                                        gACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACg                          >  2:3321793/1‑150 (MQ=255)
                                                                                                                        gACCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCAGAAAAAAGCCCCGACGAGACg                          >  1:683297/1‑150 (MQ=255)
                                                                                                                         aCCGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgg                         >  2:4574188/1‑150 (MQ=255)
                                                                                                                          ccGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                        >  2:4021971/1‑150 (MQ=255)
                                                                                                                          ccGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACggg                        <  1:2462550/150‑1 (MQ=255)
                                                                                                                           cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgggg                       <  1:1901445/150‑1 (MQ=255)
                                                                                                                           cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgggg                       >  2:374417/1‑150 (MQ=255)
                                                                                                                           cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgggg                       >  1:1285364/1‑150 (MQ=255)
                                                                                                                           cGCATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACgggg                       >  2:964914/1‑150 (MQ=255)
                                                                                                                             cATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCt                     >  2:67920/1‑150 (MQ=255)
                                                                                                                             cATCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCt                     <  1:483474/150‑1 (MQ=255)
                                                                                                                              aTCCATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTa                    >  2:1717121/1‑150 (MQ=255)
                                                                                                                                ccATACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaaa                  >  2:2957387/1‑150 (MQ=255)
                                                                                                                                  aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCATCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc                <  2:3887877/150‑1 (MQ=255)
                                                                                                                                  aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAATGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc                >  2:5091739/1‑150 (MQ=255)
                                                                                                                                  aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAATGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc                >  2:1201154/1‑150 (MQ=255)
                                                                                                                                  aTACGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATc                >  1:4781359/1‑150 (MQ=255)
                                                                                                                                     cGCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATg             >  2:316418/1‑150 (MQ=255)
                                                                                                                                      gCACTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGa            <  1:1683029/150‑1 (MQ=255)
                                                                                                                                        aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc          >  1:2718065/1‑150 (MQ=255)
                                                                                                                                        aCTCTGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATc          >  1:1556645/1‑150 (MQ=255)
                                                                                                                                          tctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAg        <  1:4799029/150‑1 (MQ=255)
                                                                                                                                           ctGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg       >  1:2098390/1‑150 (MQ=255)
                                                                                                                                            tGCCCGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgt      <  1:4569632/150‑1 (MQ=255)
                                                                                                                                                cGTAGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGttt  <  2:3522035/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCGCTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTT  >  CP009273/2404625‑2404918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑