Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,422,841 C→A 100% D293Y (GAT→TAT)  purF ← amidophosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,422,8410CA100.0% 908.0 / ‑6.7 274D293Y (GAT→TAT) purFamidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (137/136);  total (138/136)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 274 total aligned reads displayed.

GCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCAC  >  CP009273/2422694‑2422987
                                                                                                                                                   |                                                                                                                                                  
gctgctGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAGGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATAtt                                                                                                                                                  >  1:3075198/1‑150 (MQ=255)
gctgctGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATAtt                                                                                                                                                  <  1:3253331/150‑1 (MQ=255)
  tgctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTcc                                                                                                                                                >  1:382805/1‑150 (MQ=255)
   gctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTccc                                                                                                                                               <  2:1855435/150‑1 (MQ=255)
   gctgGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTccc                                                                                                                                               <  1:593280/150‑1 (MQ=255)
        cccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTcg                                                                                                                                          <  2:1113834/150‑1 (MQ=255)
         ccGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTcgc                                                                                                                                         >  1:3448576/1‑150 (MQ=255)
          cGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTcgcg                                                                                                                                        >  2:2297291/1‑150 (MQ=255)
            gCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCggg                                                                                                                                      <  2:1211268/150‑1 (MQ=255)
             cATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGc                                                                                                                                     >  2:2504676/1‑150 (MQ=255)
                gATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAt                                                                                                                                  >  2:3816242/1‑150 (MQ=255)
                   aaaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtttt                                                                                                                               >  2:4281331/1‑150 (MQ=255)
                    aaGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTtc                                                                                                                              >  2:2811755/1‑150 (MQ=255)
                     aGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTtct                                                                                                                             >  1:1921509/1‑150 (MQ=255)
                      ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTtctc                                                                                                                            <  2:1269454/150‑1 (MQ=255)
                       gTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCg                                                                                                                           >  1:40054/1‑150 (MQ=255)
                        tGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGc                                                                                                                          >  2:1769241/1‑150 (MQ=255)
                          cGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGccc                                                                                                                        >  1:1032542/1‑150 (MQ=255)
                          cGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGccc                                                                                                                        <  1:4142741/150‑1 (MQ=255)
                           ggCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCa                                                                                                                       >  2:4870131/1‑150 (MQ=255)
                           ggCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCa                                                                                                                       <  1:167323/150‑1 (MQ=255)
                            gCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAg                                                                                                                      <  2:4535583/150‑1 (MQ=255)
                             ccAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGt                                                                                                                     >  2:3506776/1‑150 (MQ=255)
                               aaCATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGttt                                                                                                                   >  2:371212/1‑150 (MQ=255)
                                  aTAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt                                                                                                                <  1:1875268/150‑1 (MQ=255)
                                  aTAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt                                                                                                                >  1:46537/1‑150 (MQ=255)
                                  aTAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt                                                                                                                >  2:2661350/1‑150 (MQ=255)
                                   tAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTg                                                                                                               <  1:1494773/150‑1 (MQ=255)
                                          ttttAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCatat                                                                                                        <  2:5124775/150‑1 (MQ=255)
                                           tttAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATAtt                                                                                                       >  2:3871931/1‑150 (MQ=255)
                                           tttAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATAtt                                                                                                       <  2:3897295/150‑1 (MQ=255)
                                             tAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTca                                                                                                     >  1:770344/1‑150 (MQ=255)
                                               aCGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTcaca                                                                                                   <  1:2083039/150‑1 (MQ=255)
                                                cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCacac                                                                                                  >  1:3746523/1‑150 (MQ=255)
                                                cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCacac                                                                                                  >  1:830169/1‑150 (MQ=255)
                                                 gAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcg                                                                                                 <  1:1316859/150‑1 (MQ=255)
                                                  aaGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgc                                                                                                <  1:1888954/150‑1 (MQ=255)
                                                   aGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGACATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgcg                                                                                               >  2:3112225/1‑150 (MQ=255)
                                                     cccTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgcgcg                                                                                             <  2:3974909/150‑1 (MQ=255)
                                                         ggCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGt                                                                                         >  1:293297/1‑150 (MQ=255)
                                                            ggTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTaaa                                                                                      >  1:219750/1‑150 (MQ=255)
                                                                 ggTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaa                                                                                 >  2:4506872/1‑150 (MQ=255)
                                                                  gTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaaa                                                                                <  1:3248789/150‑1 (MQ=255)
                                                                  gTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaaa                                                                                <  1:4860779/150‑1 (MQ=255)
                                                                  gTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaaa                                                                                <  2:1012610/150‑1 (MQ=255)
                                                                  gTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATAGTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaaa                                                                                <  2:4084647/150‑1 (MQ=255)
                                                                      gCCCAGAATACGAGCAATGTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAAtttt                                                                            <  2:3087666/150‑1 (MQ=255)
                                                                         cAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGt                                                                                         <  2:281398/134‑1 (MQ=255)
                                                                         cAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTc                                                                         <  2:728726/150‑1 (MQ=255)
                                                                           gAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGa                                                                       >  1:3381503/1‑150 (MQ=255)
                                                                            aaTACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                      <  1:2028200/150‑1 (MQ=255)
                                                                            aaTACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                      <  2:4930426/150‑1 (MQ=255)
                                                                             aTACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATa                                                                     <  2:3302205/150‑1 (MQ=255)
                                                                             aTACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATa                                                                     >  2:652258/1‑150 (MQ=255)
                                                                              tACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATaa                                                                    <  1:4400247/150‑1 (MQ=255)
                                                                                  aGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGa                                                                <  2:3749692/150‑1 (MQ=255)
                                                                                  aGCAATGTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGa                                                                <  2:3010302/150‑1 (MQ=255)
                                                                                     aaTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTc                                                             <  1:1199724/150‑1 (MQ=255)
                                                                                      aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTcc                                                            <  2:4193818/150‑1 (MQ=255)
                                                                                      aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTcc                                                            <  2:5107823/150‑1 (MQ=255)
                                                                                         tCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcggg                                                         >  2:3682811/1‑150 (MQ=255)
                                                                                           cAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcg                                                       >  1:273797/1‑150 (MQ=255)
                                                                                            aGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcgg                                                      >  2:4142712/1‑150 (MQ=255)
                                                                                            aGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcgg                                                      <  1:3199002/150‑1 (MQ=255)
                                                                                            aGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcgg                                                      <  1:1763361/150‑1 (MQ=255)
                                                                                             gcgcGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcgggcggg                                                     <  1:2329805/150‑1 (MQ=255)
                                                                                                cgATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCaa                                                  >  2:2173415/1‑150 (MQ=255)
                                                                                                 gATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCaaa                                                 >  2:1486357/1‑150 (MQ=255)
                                                                                                   tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                               >  2:1097268/1‑150 (MQ=255)
                                                                                                   tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                               <  2:2323841/150‑1 (MQ=255)
                                                                                                   tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                               <  2:4420230/150‑1 (MQ=255)
                                                                                                   tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                               >  1:3924264/1‑150 (MQ=255)
                                                                                                   tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                               >  2:2935388/1‑150 (MQ=255)
                                                                                                   tatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                               <  2:3507451/150‑1 (MQ=255)
                                                                                                      cacaCGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGtata                                            <  2:3675835/150‑1 (MQ=255)
                                                                                                         acGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTatacat                                         <  1:4523759/150‑1 (MQ=255)
                                                                                                          cGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTatacata                                        >  2:1951680/1‑150 (MQ=255)
                                                                                                          cGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTatacata                                        >  1:731495/1‑150 (MQ=255)
                                                                                                                  cTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACa                                >  1:4575480/1‑150 (MQ=255)
                                                                                                                    gggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAgg                              <  1:4688103/150‑1 (MQ=255)
                                                                                                                    gggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAgg                              <  2:2584151/150‑1 (MQ=255)
                                                                                                                     ggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGc                             <  2:4648709/150‑1 (MQ=255)
                                                                                                                      gatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCa                            <  2:3214193/150‑1 (MQ=255)
                                                                                                                      gatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCa                            >  1:840802/1‑150 (MQ=255)
                                                                                                                         cgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACgg                         >  1:5006810/1‑150 (MQ=255)
                                                                                                                          gggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGa                        >  2:866709/1‑150 (MQ=255)
                                                                                                                           ggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGAt                       <  1:3493455/150‑1 (MQ=255)
                                                                                                                           ggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGAt                       <  2:2564318/150‑1 (MQ=255)
                                                                                                                             atcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTg                     >  2:100697/1‑150 (MQ=255)
                                                                                                                               caccacGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCt                   >  1:3758781/1‑150 (MQ=255)
                                                                                                                                     gTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCgg             >  2:1598176/1‑150 (MQ=255)
                                                                                                                                       cGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGAt           <  1:216132/150‑1 (MQ=255)
                                                                                                                                        gATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGAtt          <  1:3646749/150‑1 (MQ=255)
                                                                                                                                        gATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGAtt          >  2:1980375/1‑150 (MQ=255)
                                                                                                                                        gATATCCAGAAATTCCCATTCGCGGGCAATTTTCACGCCCAGTTTCGTGACCATATTCACACGAGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGAGGGCAAAGTATACAAACTCAAACATGCACGGATTGCTGACCGGAtt          >  2:357868/1‑150 (MQ=255)
                                                                                                                                          tatCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt        <  1:2635805/150‑1 (MQ=255)
                                                                                                                                           atCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc       >  2:82928/1‑150 (MQ=255)
                                                                                                                                              cAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGc    >  1:3334360/1‑150 (MQ=255)
                                                                                                                                               aGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGca   >  1:4241788/1‑150 (MQ=255)
                                                                                                                                                gATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGcac  <  1:2564905/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGCTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCAGCAC  >  CP009273/2422694‑2422987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑