Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,424,074 C→T 100% D57N (GAC→AAC)  cvpA ← colicin V production membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,424,0740CT100.0% 940.1 / NA 278D57N (GAC→AAC) cvpAcolicin V production membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (136/142);  total (136/142)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 278 total aligned reads displayed.

ACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTA  >  CP009273/2423928‑2424220
                                                                                                                                                  |                                                                                                                                                  
 ccGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCa                                                                                                                                                <  2:2995866/150‑1 (MQ=255)
     aaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGc                                                                                                                                            <  1:4982363/150‑1 (MQ=255)
     aaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGc                                                                                                                                            >  2:3691619/1‑150 (MQ=255)
       cAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGccc                                                                                                                                          <  1:1498504/150‑1 (MQ=255)
       cAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGccc                                                                                                                                          <  1:3806267/150‑1 (MQ=255)
       cAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGccc                                                                                                                                          >  2:4839271/1‑150 (MQ=255)
        aGACGCCCAGCAGCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGATCCAGTTCGTTTTCAAAGccca                                                                                                                                         <  1:4346336/150‑2 (MQ=255)
        aGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCg                                                                                                                                         >  1:3880912/1‑150 (MQ=255)
        aGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCg                                                                                                                                         <  2:4074492/150‑1 (MQ=255)
           cGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTaa                                                                                                                                      <  2:1579577/150‑1 (MQ=255)
           cGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTaa                                                                                                                                      <  1:2310484/150‑1 (MQ=255)
              ccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCTTTTTCAAAGCGCGTacaca                                                                                                                                   >  2:5080323/1‑149 (MQ=255)
                aGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                                 >  2:327388/1‑150 (MQ=255)
                aGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                                 <  2:2851100/150‑1 (MQ=255)
                   aCCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACt                                                                                                                              >  1:1256468/1‑150 (MQ=255)
                   aCCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACt                                                                                                                              <  1:3145490/150‑1 (MQ=255)
                     ccGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGAAAACCAGACTGa                                                                                                                            >  1:605170/1‑150 (MQ=255)
                      cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAc                                                                                                                           >  2:2948435/1‑150 (MQ=255)
                      cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAc                                                                                                                           <  1:4531880/150‑1 (MQ=255)
                      cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAc                                                                                                                           >  2:2466322/1‑150 (MQ=255)
                      cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAc                                                                                                                           >  1:1846317/1‑150 (MQ=255)
                      cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAc                                                                                                                           <  2:4237773/150‑1 (MQ=255)
                       gATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTAGCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACa                                                                                                                          <  2:4363812/150‑1 (MQ=255)
                           ggTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTa                                                                                                                      <  1:2403380/150‑1 (MQ=255)
                            gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTaa                                                                                                                     <  2:4423913/150‑1 (MQ=255)
                            gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTaa                                                                                                                     <  2:3896125/150‑1 (MQ=255)
                             tGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                                                    <  2:844029/150‑1 (MQ=255)
                              gCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgt                                                                                                                   >  2:3420747/1‑150 (MQ=255)
                               ccTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtg                                                                                                                  <  1:1587646/150‑1 (MQ=255)
                                  gACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtag                                                                                                               <  1:4665075/150‑1 (MQ=255)
                                   aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagt                                                                                                              <  2:2658901/150‑1 (MQ=255)
                                   aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagt                                                                                                              >  2:1334098/1‑150 (MQ=255)
                                   aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagt                                                                                                              <  1:2530426/150‑1 (MQ=255)
                                    cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTGTCAAAGCCCGTAAACCAGACTGACAGGGAAGTgtcgta                                                                                                             >  1:4092110/1‑150 (MQ=255)
                                     aaCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTaa                                                                                                            >  2:3861696/1‑150 (MQ=255)
                                        cccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGa                                                                                                         <  1:2347579/150‑1 (MQ=255)
                                           gTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTg                                                                                                      >  1:4471163/1‑150 (MQ=255)
                                           gTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTg                                                                                                      >  1:5158649/1‑150 (MQ=255)
                                             tttCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGc                                                                                                    >  1:2708349/1‑150 (MQ=255)
                                               tctcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaa                                                                                                  >  2:1510190/1‑150 (MQ=255)
                                                  ccaccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaacaa                                                                                               <  2:360704/150‑1 (MQ=255)
                                                   caccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGGGTAGTAATGACTGGCAACaaa                                                                                              >  2:2270795/1‑150 (MQ=255)
                                                     ccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaaga                                                                                            >  2:617281/1‑150 (MQ=255)
                                                       aaCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaaa                                                                                          <  2:3787680/150‑1 (MQ=255)
                                                          tGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGca                                                                                       <  1:4045285/150‑1 (MQ=255)
                                                          tGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGca                                                                                       <  1:4923646/150‑1 (MQ=255)
                                                          tGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGca                                                                                       >  2:1123006/1‑150 (MQ=255)
                                                             ccTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACaa                                                                                    >  2:364239/1‑150 (MQ=255)
                                                             ccTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGAGATGGCAATCCCATTTCGAAACAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACaa                                                                                    <  1:1996821/150‑1 (MQ=255)
                                                              cTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAc                                                                                   >  2:4611146/1‑150 (MQ=255)
                                                                  cACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCa                                                                               >  2:3047751/1‑150 (MQ=255)
                                                                  cACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCa                                                                               <  1:5074838/150‑1 (MQ=255)
                                                                      taGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACATTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTc                                                                           >  1:2868449/2‑150 (MQ=255)
                                                                      aaGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTc                                                                           >  1:957509/1‑150 (MQ=255)
                                                                      aaGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTc                                                                           >  2:2691864/1‑150 (MQ=255)
                                                                       aGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                                                          >  1:2060516/1‑150 (MQ=255)
                                                                        gTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAc                                                                         >  2:1394787/1‑150 (MQ=255)
                                                                         ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAcc                                                                        >  2:3159679/1‑150 (MQ=255)
                                                                             cGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCg                                                                    >  2:2596162/1‑150 (MQ=255)
                                                                             cGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCg                                                                    <  1:267765/150‑1 (MQ=255)
                                                                               aTAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAt                                                                  <  2:1000153/150‑1 (MQ=255)
                                                                               aTAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAt                                                                  >  2:225295/1‑150 (MQ=255)
                                                                                   cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACg                                                              <  2:4802832/150‑1 (MQ=255)
                                                                                   cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACg                                                              >  1:3992437/1‑150 (MQ=255)
                                                                                      cAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCtt                                                           >  2:125620/1‑150 (MQ=255)
                                                                                      cAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCtt                                                           >  2:4666540/1‑150 (MQ=255)
                                                                                      cAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCtt                                                           <  1:3134645/150‑1 (MQ=255)
                                                                                       aaCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTc                                                          <  1:1033570/150‑1 (MQ=255)
                                                                                       aaCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTc                                                          <  2:2189142/150‑1 (MQ=255)
                                                                                          gATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACg                                                       <  1:911564/150‑1 (MQ=255)
                                                                                                 gTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAg                                                <  1:3564697/150‑1 (MQ=255)
                                                                                                  tAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGc                                               >  2:3185913/1‑150 (MQ=255)
                                                                                                      gATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcgcg                                           <  1:1005773/150‑1 (MQ=255)
                                                                                                         aaaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                        >  2:67201/1‑150 (MQ=255)
                                                                                                         aaaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                        <  2:1840896/150‑1 (MQ=255)
                                                                                                         aaaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGAACAACCCCATGTCACCAGCGAGAACGCTTCACGAACAAAGCCGCGGAt                                        >  2:2005196/1‑150 (MQ=255)
                                                                                                          aaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc                                       >  2:1211979/1‑150 (MQ=255)
                                                                                                          aaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc                                       <  1:1083907/150‑1 (MQ=255)
                                                                                                           aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCa                                      >  2:1640723/1‑150 (MQ=255)
                                                                                                            cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAg                                     <  1:718224/150‑1 (MQ=255)
                                                                                                            cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGGGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAg                                     >  1:3914713/1‑150 (MQ=255)
                                                                                                              gTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGc                                   >  1:5025776/1‑150 (MQ=255)
                                                                                                                      aTGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCaga                           >  2:4970233/1‑150 (MQ=255)
                                                                                                                        ggCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagaga                         <  1:363974/150‑1 (MQ=255)
                                                                                                                         gCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagagag                        <  1:618485/150‑1 (MQ=255)
                                                                                                                             tCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaa                    <  1:2758692/150‑1 (MQ=255)
                                                                                                                             tCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaa                    <  1:1216890/150‑1 (MQ=255)
                                                                                                                              cccATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaaa                   >  2:1930114/1‑150 (MQ=255)
                                                                                                                              cccATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaaa                   <  2:1059420/150‑1 (MQ=255)
                                                                                                                               ccATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaaaa                  <  1:3302839/150‑1 (MQ=255)
                                                                                                                                cATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAg                 <  2:3554035/150‑1 (MQ=255)
                                                                                                                                  tttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCa               <  1:2455488/150‑1 (MQ=255)
                                                                                                                                    tCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAAt             >  1:2163636/1‑150 (MQ=255)
                                                                                                                                      gAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCa           <  2:4461062/150‑1 (MQ=255)
                                                                                                                                       aaCCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCAc          <  1:712651/150‑1 (MQ=255)
                                                                                                                                         ccAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACcg        <  1:533370/150‑1 (MQ=255)
                                                                                                                                             ttCGTTTTCAAAGCCCGTACACCAGACTGACAGGTAAGTGTTGTAATGACTGGCTACAAAGAAAGCACAACCGCATGTCACCAGCTATAACGATTCAAGAACAAAGCCGCGGATCAGGCTAACCTGAGAGTAAAAAGCAATCACCGCGAt    <  2:5163642/150‑1 (MQ=255)
                                                                                                                                             ttCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAt    <  1:3040578/150‑1 (MQ=255)
                                                                                                                                              tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAtt   <  1:2487410/150‑1 (MQ=255)
                                                                                                                                              tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAtt   <  2:2060516/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTA  >  CP009273/2423928‑2424220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑