Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 214,084 G→T 100% G393G (GGC→GGA)  proS ← prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273214,0840GT99.0% 967.9 / ‑1.6 292G393G (GGC→GGA) proSprolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (131/158);  total (133/159)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 303 total aligned reads displayed.

ACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAA  >  CP009273/213937‑214229
                                                                                                                                                   |                                                                                                                                                 
aCCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtcc                                                                                                                                                 <  2:2774666/150‑1 (MQ=255)
       gTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccacc                                                                                                                                          <  1:2873324/150‑1 (MQ=255)
       gTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccacc                                                                                                                                          <  1:2459290/150‑1 (MQ=255)
         cAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCcaccac                                                                                                                                        >  2:1675761/1‑150 (MQ=255)
         cAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCcaccac                                                                                                                                        >  2:2684012/1‑150 (MQ=255)
          aGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       >  1:3893558/1‑150 (MQ=255)
          aGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       >  1:677757/1‑150 (MQ=255)
            gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                     >  2:3845515/1‑150 (MQ=255)
            gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                     >  2:2212975/1‑150 (MQ=255)
            gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                     <  1:199551/150‑1 (MQ=255)
                 ggTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGa                                                                                                                                >  1:2336138/1‑150 (MQ=255)
                 ggTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGa                                                                                                                                <  1:3473774/150‑1 (MQ=255)
                    tACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGatat                                                                                                                             >  1:1749803/1‑150 (MQ=255)
                      cGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCt                                                                                                                           <  2:3597833/150‑1 (MQ=255)
                        gCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGc                                                                                                                         >  1:2149819/1‑150 (MQ=255)
                        gCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGc                                                                                                                         >  1:4546705/1‑150 (MQ=255)
                         ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTCCCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGAGATCTGCa                                                                                                                        >  2:3075842/1‑150 (MQ=255)
                         ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCa                                                                                                                        >  2:73960/1‑150 (MQ=255)
                         ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCa                                                                                                                        <  2:3169010/150‑1 (MQ=255)
                            tCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACt                                                                                                                     >  1:520304/1‑150 (MQ=255)
                             cTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACtt                                                                                                                    >  1:1485957/1‑150 (MQ=255)
                              ttCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTc                                                                                                                   <  1:443024/150‑1 (MQ=255)
                                      gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAg                                                                                                           <  2:5099231/150‑1 (MQ=255)
                                       tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                          <  2:1775277/150‑1 (MQ=255)
                                       tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                          <  1:2536559/150‑1 (MQ=255)
                                       tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                          <  2:1426098/150‑1 (MQ=255)
                                        aCGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCg                                                                                                         <  2:2494921/150‑1 (MQ=255)
                                         cGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                                                                                                        >  1:1416609/1‑150 (MQ=255)
                                          ggAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAc                                                                                                       <  1:4239737/150‑1 (MQ=255)
                                             ggCTTACAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATc                                                                                                    >  1:870037/1‑150 (MQ=255)
                                              gCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcg                                                                                                   <  1:3002890/150‑1 (MQ=255)
                                                tttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                                                                                                 <  1:11731/150‑1 (MQ=255)
                                                tttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                                                                                                 <  1:2634099/150‑1 (MQ=255)
                                                 ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                                >  2:4158818/1‑150 (MQ=255)
                                                   cAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATc                                                                                              <  1:597065/150‑1 (MQ=255)
                                                   cAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATc                                                                                              <  2:247459/150‑1 (MQ=255)
                                                    aGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc                                                                                             >  2:3930293/1‑150 (MQ=255)
                                                     gTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCTGCCTACCCTGGCCAGCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAAATCGCGATcca                                                                                            <  1:3309817/150‑2 (MQ=255)
                                                     gTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATccc                                                                                            <  1:3864745/150‑1 (MQ=255)
                                                      tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCa                                                                                           <  1:1363115/150‑1 (MQ=255)
                                                        cTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt                                                                                         <  2:51939/150‑1 (MQ=255)
                                                         ttCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGtt                                                                                        <  1:3135624/150‑1 (MQ=255)
                                                         ttCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAGCCCAGtt                                                                                        <  1:4791047/150‑1 (MQ=255)
                                                           cGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                      <  2:1881531/150‑1 (MQ=255)
                                                           cGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                      >  1:4527843/1‑150 (MQ=255)
                                                           cGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                      <  2:2547619/150‑1 (MQ=255)
                                                            ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt                                                                                     <  2:3334117/150‑1 (MQ=255)
                                                             gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGATTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg                                                                                    <  1:1661212/150‑1 (MQ=255)
                                                               gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGcc                                                                                  <  1:1545059/150‑1 (MQ=255)
                                                               gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGcc                                                                                  <  1:728784/150‑1 (MQ=255)
                                                                tACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGGTACGGCTATCTGCAACTTCCGGGGTAGCGACATCGCGATCGCAGTTGATGCCg                                                                                 >  2:4328579/1‑150 (MQ=255)
                                                                 aCTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGa                                                                                >  1:530273/1‑150 (MQ=255)
                                                                   ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAag                                                                              <  1:3620123/150‑1 (MQ=255)
                                                                   ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAag                                                                              <  2:4584177/150‑1 (MQ=255)
                                                                    tGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagt                                                                             <  2:4681420/150‑1 (MQ=255)
                                                                      gTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagtag                                                                           <  2:3110314/150‑1 (MQ=255)
                                                                       tACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagtagt                                                                          <  2:4291856/150‑1 (MQ=255)
                                                                        aCCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                         <  1:4121756/150‑1 (MQ=255)
                                                                         cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTCGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtgt                                                                        >  2:2852918/1‑150 (MQ=255)
                                                                          ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGtt                                                                       <  1:3058540/150‑1 (MQ=255)
                                                                           cAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGttt                                                                      >  2:1534403/1‑150 (MQ=255)
                                                                                 gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATc                                                                >  2:19865/1‑150 (MQ=255)
                                                                                   aGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                              <  1:4643286/150‑1 (MQ=255)
                                                                                   aGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                              <  2:527936/150‑1 (MQ=255)
                                                                                    gATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGAt                                                             >  2:1118058/1‑150 (MQ=255)
                                                                                    gATGTGAACAACTTCGATACCACGTTTTATAAGCAGACTACACTGGCCATCCGTGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATAGCGATCCCAGTTGATGCATAAGTAGTGTTTACCATCTAt                                                             <  2:1766692/150‑1 (MQ=255)
                                                                                       gtgACCAACTTCGATACCACGTTTGATCAGCATCCTACCCTGGCCATCCGGGCTTGGATCTCCAGACACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGtt                                                          <  1:3981666/150‑1 (MQ=255)
                                                                                        tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                                                         >  1:1158569/1‑150 (MQ=255)
                                                                                           ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCa                                                      >  1:192154/1‑150 (MQ=255)
                                                                                               cTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcag                                                  <  2:19322/150‑1 (MQ=255)
                                                                                                 tCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcagca                                                >  2:5034832/1‑150 (MQ=255)
                                                                                                  cGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcagcag                                               <  2:1013712/150‑1 (MQ=255)
                                                                                                          cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCa                                       <  1:1613711/150‑1 (MQ=255)
                                                                                                          cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCa                                       <  2:2267362/150‑1 (MQ=255)
                                                                                                           gTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAc                                      >  2:69310/1‑150 (MQ=255)
                                                                                                           gTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAc                                      >  2:3809780/1‑150 (MQ=255)
                                                                                                             ttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTc                                    >  2:4027541/1‑150 (MQ=255)
                                                                                                                aTCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATc                                 <  1:2284582/150‑1 (MQ=255)
                                                                                                                      agcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                           >  1:4089193/1‑150 (MQ=255)
                                                                                                                      agcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                           <  2:2374949/150‑1 (MQ=255)
                                                                                                                             ccTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGGTCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtac                    >  2:2057143/1‑150 (MQ=255)
                                                                                                                             ccTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtac                    <  2:3262771/150‑1 (MQ=255)
                                                                                                                              cTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacg                   >  1:1861633/1‑150 (MQ=255)
                                                                                                                              cTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacg                   <  1:1324776/150‑1 (MQ=255)
                                                                                                                                 gCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTc                <  1:82914/150‑1 (MQ=255)
                                                                                                                                  ccATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCa               <  1:49799/150‑1 (MQ=255)
                                                                                                                                  ccATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCa               <  2:4819954/150‑1 (MQ=255)
                                                                                                                                  ccATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCa               <  1:460159/150‑1 (MQ=255)
                                                                                                                                    aTCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt             <  2:3387193/150‑1 (MQ=255)
                                                                                                                                    aTCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt             >  1:4347216/1‑150 (MQ=255)
                                                                                                                                     tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATc            <  1:3746177/150‑1 (MQ=255)
                                                                                                                                     tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATc            <  2:530273/150‑1 (MQ=255)
                                                                                                                                        gggCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacc         >  1:4134014/1‑150 (MQ=255)
                                                                                                                                         ggCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACTGTCAATcacca        >  2:3440292/1‑150 (MQ=255)
                                                                                                                                         ggCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacca        >  1:5129956/1‑150 (MQ=255)
                                                                                                                                           cTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCaccacc      >  2:4615778/1‑150 (MQ=255)
                                                                                                                                           cTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCaccacc      <  2:3615117/150‑1 (MQ=255)
                                                                                                                                            ttGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCg     <  2:126066/150‑1 (MQ=255)
                                                                                                                                            ttGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCg     >  1:276294/1‑150 (MQ=255)
                                                                                                                                            ttGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCg     >  2:2136930/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAA  >  CP009273/213937‑214229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑