Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,499,568 C→A 100% D182Y (GAC→TAC)  ypdF ← Xaa‑Pro aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,499,5680CA98.1% 1024.6 / 7.2 322D182Y (GAC→TAC) ypdFXaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (137/177);  total (141/181)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 326 total aligned reads displayed.

TCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCA  >  CP009273/2499421‑2499714
                                                                                                                                                   |                                                                                                                                                  
   cctgCAAGGTGCGATTCATATAAGAGCAGTAGCCCTGATACAGCGCACAGACATCGATAGTGACAAACTCGACAGCTGCAAAAATCTTGTCGCTGTCTTTGCCGTGCGGCAGAGACCCACGCCAGCCACTGGCGACAATGGTGTATAAag                                                                                                                                               <  1:643897/147‑1 (MQ=255)
   ccAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACACTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAag                                                                                                                                               <  2:4876756/150‑1 (MQ=255)
    acgCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAaga                                                                                                                                              <  2:1696514/148‑1 (MQ=255)
     aGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                             >  1:3167785/1‑150 (MQ=255)
      gCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAgg                                                                                                                                            <  2:3146390/150‑1 (MQ=255)
      gCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAgg                                                                                                                                            <  1:1917480/150‑1 (MQ=255)
          ggTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCttt                                                                                                                                        <  1:1019278/150‑1 (MQ=255)
           gTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCtttt                                                                                                                                       <  2:623159/150‑1 (MQ=255)
              cgcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCt                                                                                                                                    <  2:2346362/150‑1 (MQ=255)
               gcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAACCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCCCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTg                                                                                                                                   <  2:3654030/150‑1 (MQ=255)
               gcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTg                                                                                                                                   <  1:2221050/150‑1 (MQ=255)
               gcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTg                                                                                                                                   <  1:802232/150‑1 (MQ=255)
                cgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTgc                                                                                                                                  <  2:4472105/150‑1 (MQ=255)
                 gTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTgcg                                                                                                                                 >  2:1987788/1‑150 (MQ=255)
                   cATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAGAAAGAGGCTTTTTCTGCGcc                                                                                                                               >  1:2696824/1‑150 (MQ=255)
                     tatCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCct                                                                                                                             <  1:1973053/150‑1 (MQ=255)
                     tatCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCct                                                                                                                             <  1:1366260/150‑1 (MQ=255)
                      atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctg                                                                                                                            >  2:898236/1‑150 (MQ=255)
                        cAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgct                                                                                                                          <  2:176736/150‑1 (MQ=255)
                          gagCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTgg                                                                                                                        <  2:2580038/150‑1 (MQ=255)
                             cAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGgcgc                                                                                                                     >  2:4527738/1‑150 (MQ=255)
                              aGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCa                                                                                                                    <  2:3336275/150‑1 (MQ=255)
                              aGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCa                                                                                                                    >  1:3225220/1‑150 (MQ=255)
                                tAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCTGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATa                                                                                                                  <  2:3370430/150‑1 (MQ=255)
                                 aGCCCTGATACTGCGCACCGAAATCGAGAGTGACCAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACTATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaa                                                                                                                 <  2:4773040/150‑1 (MQ=255)
                                 aGCCCTGAGACCGCGCACCGAAATCGAGCGGGACACCCTCGCCCGCGGCAACACTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATaa                                                                                                                 <  2:2919187/150‑1 (MQ=255)
                                     cTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCa                                                                                                             >  1:2494281/1‑150 (MQ=255)
                                      tGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCAc                                                                                                            <  1:2957509/150‑1 (MQ=255)
                                       gATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACt                                                                                                           <  1:4090719/150‑1 (MQ=255)
                                               gcACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCa                                                                                                   >  1:3293841/1‑150 (MQ=255)
                                                cACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAg                                                                                                  <  2:3050694/150‑1 (MQ=255)
                                                cACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAg                                                                                                  <  2:4715359/150‑1 (MQ=255)
                                                cACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAg                                                                                                  >  2:4570312/1‑150 (MQ=255)
                                                  ccGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCg                                                                                                <  2:818806/150‑1 (MQ=255)
                                                   cGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCgg                                                                                               >  1:4459226/1‑150 (MQ=255)
                                                     aattCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCt                                                                                             <  1:2009002/147‑1 (MQ=255)
                                                     aaaTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCt                                                                                             <  1:4577688/150‑1 (MQ=255)
                                                     aaaTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCAGAAACCACTCCAGTTCAGAGGCt                                                                                             >  1:355099/1‑150 (MQ=255)
                                                      aaTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTa                                                                                            <  1:3012358/150‑1 (MQ=255)
                                                       aTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGACTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                           >  1:549549/1‑150 (MQ=255)
                                                          gagaGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAtctc                                                                                        <  2:321854/150‑1 (MQ=255)
                                                          gagaGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAtctc                                                                                        >  2:249451/1‑150 (MQ=255)
                                                           agagTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCACCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTcg                                                                                       <  2:1622352/150‑1 (MQ=255)
                                                               tGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgct                                                                                   <  2:2034324/150‑1 (MQ=255)
                                                                gACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                                  <  1:587884/150‑1 (MQ=255)
                                                                gACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                                  <  2:407099/150‑1 (MQ=255)
                                                                gACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                                  <  1:736242/150‑1 (MQ=255)
                                                                gACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                                  >  2:1902873/1‑150 (MQ=255)
                                                                  cAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgc                                                                                >  2:210327/1‑150 (MQ=255)
                                                                   aaaCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                               <  2:1153314/150‑1 (MQ=255)
                                                                     aCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCa                                                                             <  2:3706389/150‑1 (MQ=255)
                                                                        cGCCCGCTGCAACAAACTTGTCGCTGGCTTTTCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcc                                                                          >  1:4417588/1‑150 (MQ=255)
                                                                           ccGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCg                                                                       >  1:4268682/1‑150 (MQ=255)
                                                                            cGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACACTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGc                                                                      >  2:5095175/1‑150 (MQ=255)
                                                                             gCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGcc                                                                     <  2:2689351/150‑1 (MQ=255)
                                                                             gCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGcc                                                                     <  1:969648/150‑1 (MQ=255)
                                                                               tGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTg                                                                   >  1:4961009/1‑150 (MQ=255)
                                                                                          tGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAgcggc                                                        <  2:4950954/150‑1 (MQ=255)
                                                                                          tGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAgcggc                                                        >  2:4582761/1‑150 (MQ=255)
                                                                                            tCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGa                                                      <  1:3688058/150‑1 (MQ=255)
                                                                                            tCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGa                                                      <  1:1231858/150‑1 (MQ=255)
                                                                                            tCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGa                                                      <  1:4309745/150‑1 (MQ=255)
                                                                                            tCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGa                                                      <  1:77484/150‑1 (MQ=255)
                                                                                             cGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGaa                                                     <  2:1318774/150‑1 (MQ=255)
                                                                                              gCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAat                                                    <  2:574310/150‑1 (MQ=255)
                                                                                                tGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAatat                                                  >  1:5108018/1‑150 (MQ=255)
                                                                                                    tttGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGctc                                              >  2:3886166/1‑150 (MQ=255)
                                                                                                         cGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCAc                                         <  1:2844314/150‑1 (MQ=255)
                                                                                                          gTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCAcc                                        <  1:3984003/150‑1 (MQ=255)
                                                                                                            gCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACcgc                                      >  1:1641375/1‑150 (MQ=255)
                                                                                                              ggCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGa                                    >  2:1068087/1‑150 (MQ=255)
                                                                                                                 aGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCa                                 <  1:2154702/150‑1 (MQ=255)
                                                                                                                  gcgcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAg                                <  1:4209303/150‑1 (MQ=255)
                                                                                                                  gcgcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAg                                >  2:1590191/1‑150 (MQ=255)
                                                                                                                    gcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCa                              <  2:2327603/150‑1 (MQ=255)
                                                                                                                        cACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                          <  2:2774189/150‑1 (MQ=255)
                                                                                                                        cACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                          >  2:4877235/1‑150 (MQ=255)
                                                                                                                         aCGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATccc                         <  2:1956410/150‑1 (MQ=255)
                                                                                                                          cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCca                        <  2:2187837/150‑1 (MQ=255)
                                                                                                                            ccagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCcaca                      >  1:1760535/1‑150 (MQ=255)
                                                                                                                            ccagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCcaca                      <  2:3493838/150‑1 (MQ=255)
                                                                                                                             cagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATACACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                     <  2:2257783/150‑1 (MQ=255)
                                                                                                                             cagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                     >  1:3420473/1‑150 (MQ=255)
                                                                                                                             cagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                     >  1:3062269/1‑150 (MQ=255)
                                                                                                                             cagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                     <  1:2610572/150‑1 (MQ=255)
                                                                                                                             cagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                     >  1:2912384/1‑150 (MQ=255)
                                                                                                                                  aCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgag                <  2:1496893/150‑1 (MQ=255)
                                                                                                                                   cTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgagg               <  1:1556906/150‑1 (MQ=255)
                                                                                                                                    tGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgaggc              >  2:4201261/1‑150 (MQ=255)
                                                                                                                                     ggCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgaggcg             <  1:3362146/150‑1 (MQ=255)
                                                                                                                                     ggCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgaggcg             <  1:3898885/150‑1 (MQ=255)
                                                                                                                                       cGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGa           >  1:448670/1‑150 (MQ=255)
                                                                                                                                         aCAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAtt         <  1:474744/150‑1 (MQ=255)
                                                                                                                                          cAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAttt        <  1:2350424/150‑1 (MQ=255)
                                                                                                                                          cAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAttt        >  1:4786996/1‑150 (MQ=255)
                                                                                                                                           aaTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAtttt       >  1:4985914/1‑150 (MQ=255)
                                                                                                                                            aTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtc      <  2:1550725/150‑1 (MQ=255)
                                                                                                                                              ggTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtctc    >  2:884730/1‑150 (MQ=255)
                                                                                                                                               gtgtAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCGGCAATCCCACAGGCGAGGCGGATTTTCTcc   >  1:3771554/1‑150 (MQ=255)
                                                                                                                                               gtgtAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTcc   >  1:1409986/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCCA  >  CP009273/2499421‑2499714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑