Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,567,211 C→A 100% D152Y (GAT→TAT)  eutT ← cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,567,2110CA98.0% 1076.3 / 8.7 342D152Y (GAT→TAT) eutTcobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base C (5/2);  new base A (175/160);  total (180/162)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 342 total aligned reads displayed.

TGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGA  >  CP009273/2567064‑2567357
                                                                                                                                                   |                                                                                                                                                  
tGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAgg                                                                                                                                                  >  1:4932223/1‑150 (MQ=255)
 gAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgc                                                                                                                                                 >  2:4068889/1‑150 (MQ=255)
  aGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcg                                                                                                                                                >  2:1162303/1‑150 (MQ=255)
    cAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGTAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcgcg                                                                                                                                              >  2:3589553/1‑150 (MQ=255)
    cAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGgcgcg                                                                                                                                              >  1:1168236/1‑150 (MQ=255)
      aCGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCa                                                                                                                                            <  1:269074/150‑1 (MQ=255)
       cGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCAt                                                                                                                                           >  2:811323/1‑150 (MQ=255)
              tCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTg                                                                                                                                    <  1:2995675/150‑1 (MQ=255)
               cgcgCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGc                                                                                                                                   <  2:3320538/150‑1 (MQ=255)
                 cgcCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGGGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCa                                                                                                                                 >  1:2639821/1‑150 (MQ=255)
                  gcCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCaa                                                                                                                                <  2:1987439/150‑1 (MQ=255)
                  gcCCGTGGCTGGCTTCCGGCACCAGATGATCGGGGTCGAGATAGCGCAGCGGCTGAGGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCCTAGGCGCGCATCCTGTTGccca                                                                                                                                >  2:2675771/1‑148 (MQ=255)
                   cccGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCCTAGGCGCGCATAATGTTGCCAAg                                                                                                                               >  2:784308/1‑150 (MQ=255)
                   cccGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAg                                                                                                                               <  1:606100/150‑1 (MQ=255)
                      gTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGAcg                                                                                                                            <  2:4233218/150‑1 (MQ=255)
                        ggctggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGAcgcg                                                                                                                          <  1:1020503/150‑1 (MQ=255)
                            ggctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACg                                                                                                                      <  1:4411710/150‑1 (MQ=255)
                             gctTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACgg                                                                                                                     >  1:1690414/1‑150 (MQ=255)
                               ttCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGat                                                                                                                   >  1:1917016/1‑150 (MQ=255)
                                   ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATcc                                                                                                               <  1:4185685/150‑1 (MQ=255)
                                   ggCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATcc                                                                                                               >  1:3895893/1‑150 (MQ=255)
                                       ccAGATGATCGTGGTCTAGATAGCGCACCTCCTGATGAGAAAGCCGATGCAGATCTTCCTCAGAAAGCCCGACAAACGCCAGGCAGCCCAGCGGTAACCCCATCGCATAGGCGCGCATAATTAAGCCAAGACGCGAACGGATATCCgcca                                                                                                           >  2:2214859/1‑150 (MQ=255)
                                       ccAGAGGATCGTGGTCGAGATAGCGGAGCGTCTTATGAGAAAGCCGCTGCAGATCTTCGTCAGAAAGCCCGTCCATCGCCTGGCAGCCCAGCGTTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgcca                                                                                                           <  2:1505306/150‑1 (MQ=255)
                                        cAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgccag                                                                                                          >  2:2984494/1‑150 (MQ=255)
                                         aGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGAc                                                                                                                             <  1:1123914/130‑1 (MQ=255)
                                          gatgatCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgccagcc                                                                                                        >  1:2141015/1‑150 (MQ=255)
                                           atgatCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgccagcca                                                                                                       >  2:4956403/1‑150 (MQ=255)
                                           atgatCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCgccagcca                                                                                                       <  1:2812919/150‑1 (MQ=255)
                                               tCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGc                                                                                                   >  1:162454/1‑150 (MQ=255)
                                                 gTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTg                                                                                                 >  1:911674/1‑150 (MQ=255)
                                                 gTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTg                                                                                                 >  2:3424320/1‑150 (MQ=255)
                                                  tGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGc                                                                                                <  1:5027024/150‑1 (MQ=255)
                                                  tGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGc                                                                                                <  2:5013932/150‑1 (MQ=255)
                                                    gTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCa                                                                                              <  1:4666828/150‑1 (MQ=255)
                                                     tCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCAc                                                                                             <  1:1770065/150‑1 (MQ=255)
                                                     tCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCAc                                                                                             >  1:4537514/1‑150 (MQ=255)
                                                      cGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACg                                                                                            <  1:4127224/150‑1 (MQ=255)
                                                       gagaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACgg                                                                                           <  2:1916914/150‑1 (MQ=255)
                                                       gagaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACgg                                                                                           >  1:351591/1‑150 (MQ=255)
                                                        agaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGt                                                                                          <  1:3283744/150‑1 (MQ=255)
                                                         gaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGtt                                                                                         <  2:1271640/150‑1 (MQ=255)
                                                         gaTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGtt                                                                                         >  2:3344290/1‑150 (MQ=255)
                                                          aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTc                                                                                        <  2:4193322/150‑1 (MQ=255)
                                                          aTAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTc                                                                                        <  1:2921218/150‑1 (MQ=255)
                                                           tAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTcc                                                                                       <  1:3998249/150‑1 (MQ=255)
                                                           tAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTcc                                                                                       >  2:2342643/1‑150 (MQ=255)
                                                           tAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTcc                                                                                       >  2:3675464/1‑150 (MQ=255)
                                                                   cGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTc                                                                               <  2:4474548/150‑1 (MQ=255)
                                                                   cGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTc                                                                               >  2:2515581/1‑150 (MQ=255)
                                                                   cGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTc                                                                               <  1:3579607/150‑1 (MQ=255)
                                                                      cTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAt                                                                            >  1:4817431/1‑150 (MQ=255)
                                                                      cTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAt                                                                            <  2:3954775/150‑1 (MQ=255)
                                                                        gatgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAttt                                                                          >  1:2102989/1‑150 (MQ=255)
                                                                        gatgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAAttt                                                                          >  1:3369969/1‑150 (MQ=255)
                                                                         atgaGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTg                                                                         >  1:4040133/1‑150 (MQ=255)
                                                                           gagaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCa                                                                       >  1:5118981/1‑150 (MQ=255)
                                                                            agaAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAg                                                                      <  1:3179950/150‑1 (MQ=255)
                                                                                   cGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACt                                                               <  2:4343545/150‑1 (MQ=255)
                                                                                   cGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACt                                                               <  2:710662/150‑1 (MQ=255)
                                                                                    gATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTg                                                              <  1:4289643/150‑1 (MQ=255)
                                                                                      tGCAGATCTTCGTCAGAAAGCCCGACAAGCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGcc                                                            <  2:5161970/150‑1 (MQ=255)
                                                                                       gCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCa                                                           >  2:1479378/1‑150 (MQ=255)
                                                                                         aGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAgc                                                         >  2:4603754/1‑150 (MQ=255)
                                                                                            tCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGACCGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTGCAATTTGCAGCCACACTGCCAGCGCa                                                      >  2:157085/1‑150 (MQ=255)
                                                                                              ttCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAAt                                                    <  2:2081896/150‑1 (MQ=255)
                                                                                               tCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATg                                                   >  1:4383758/1‑150 (MQ=255)
                                                                                                  tCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTg                                                >  2:4112384/1‑150 (MQ=255)
                                                                                                     gAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTg                                             <  2:4862540/150‑1 (MQ=255)
                                                                                                        aGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCg                                          >  1:2495902/1‑150 (MQ=255)
                                                                                                         gCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCga                                         >  2:3817777/1‑150 (MQ=255)
                                                                                                          cccGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCgag                                        <  2:188079/150‑1 (MQ=255)
                                                                                                            cGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGaa                                      <  1:3072924/150‑1 (MQ=255)
                                                                                                             gACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAAc                                     >  2:1384702/1‑150 (MQ=255)
                                                                                                                aaTCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgc                                  >  1:1504417/1‑150 (MQ=255)
                                                                                                                 aTCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACcgcg                                 <  1:1342368/150‑1 (MQ=255)
                                                                                                                    gCCTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGa                              >  2:380798/1‑150 (MQ=255)
                                                                                                                     ccTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaa                             >  2:1377341/1‑150 (MQ=255)
                                                                                                                     ccTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaa                             >  1:3197501/1‑150 (MQ=255)
                                                                                                                      cTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaaa                            >  2:1070699/1‑150 (MQ=255)
                                                                                                                      cTGGCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGaaa                            >  1:732099/1‑150 (MQ=255)
                                                                                                                         gCAGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAAcc                         <  2:2284569/150‑1 (MQ=255)
                                                                                                                           aGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCa                       <  2:1762618/150‑1 (MQ=255)
                                                                                                                           aGCCCAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCa                       >  1:2205179/1‑150 (MQ=255)
                                                                                                                              ccAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAc                    <  1:184580/150‑1 (MQ=255)
                                                                                                                              ccAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAc                    >  2:1695505/1‑150 (MQ=255)
                                                                                                                               cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcg                   >  1:666761/1‑150 (MQ=255)
                                                                                                                               cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcg                   <  1:3680635/150‑1 (MQ=255)
                                                                                                                               cAGCGGTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGAcg                   >  1:3720230/1‑150 (MQ=255)
                                                                                                                                   ggTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa               >  1:786049/1‑150 (MQ=255)
                                                                                                                                   ggTTCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGa               <  1:3344838/150‑1 (MQ=255)
                                                                                                                                      tCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCg            >  2:1289356/1‑150 (MQ=255)
                                                                                                                                      tCCCCCAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCg            <  1:4262417/150‑1 (MQ=255)
                                                                                                                                       cccccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGc           >  1:2047967/1‑150 (MQ=255)
                                                                                                                                        ccccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCt          >  2:3151741/1‑150 (MQ=255)
                                                                                                                                          ccAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCttt        >  1:4489075/1‑150 (MQ=255)
                                                                                                                                           cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtttt       <  1:4321522/150‑1 (MQ=255)
                                                                                                                                           cAGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCtttt       <  1:1253841/150‑1 (MQ=255)
                                                                                                                                            aGCGCATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTg      >  2:4424897/1‑150 (MQ=255)
                                                                                                                                              cgcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCGTTTGGc    >  2:4303907/1‑150 (MQ=255)
                                                                                                                                               gcATAGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCg   <  1:994545/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGAGCAACGCAGCATCGCGCCCGTGGCTGGCTTCCGGCACCAGATGATCGTGGTCGAGATAGCGCAGCGGCTGATGAGAAAGCCGATGCAGATCTTCGTCAGAAAGCCCGACAATCGCCTGGCAGCCCAGCGGTTCCCCCAGCGCATCGGCGCGCATAATGTTGCCAAGACGCGAACGGATATCCGCCAGCCACGGCTGCCACGGTTCCGCCAGTTCAATTTGCAGCCACACTGCCAGCGCAATGGTGCTGTCGAGAACCGCGCGAAAACCCAGACGCGGATCGCTTTTGGCGA  >  CP009273/2567064‑2567357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑