| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,597,173 | T→G | 100% | L666R (CTG→CGG) | hyfB → | hydrogenase 4, membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,597,173 | 0 | T | G | 96.4% | 1201.9 / 12.3 | 316 | L666R (CTG→CGG) | hyfB | hydrogenase 4, membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (8/4); new base G (156/148); total (164/152) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 319 total aligned reads displayed.
GGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGT > CP009273/2597026‑2597319 | gCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTa < 1:2854268/150‑1 (MQ=255) cTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTaa < 2:2831537/150‑1 (MQ=255) tCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAAt > 2:1308493/1‑150 (MQ=255) agagCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCg < 1:1157771/150‑1 (MQ=255) cacaGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTAt > 1:1016889/1‑150 (MQ=255) caGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTg > 1:2662776/1‑150 (MQ=255) caGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTg > 2:153370/1‑150 (MQ=255) gAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGcc < 1:4427463/150‑1 (MQ=255) aaCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCg > 2:2083666/1‑150 (MQ=255) aaCCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCg < 2:150548/150‑1 (MQ=255) ccTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCt < 1:3347123/150‑1 (MQ=255) gggATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGaa < 2:1625463/150‑1 (MQ=255) gATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAAt < 2:2261233/150‑1 (MQ=255) aTGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATc < 1:447145/150‑1 (MQ=255) aTGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATc < 2:783043/150‑1 (MQ=255) tGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCa > 1:3878999/1‑150 (MQ=255) gAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCAc < 1:5109816/150‑1 (MQ=255) gAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCAc < 1:3203134/150‑1 (MQ=255) gCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCa < 2:3904120/150‑1 (MQ=255) cGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCAt > 1:600858/1‑150 (MQ=255) cGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCAt < 1:5013124/150‑1 (MQ=255) cGGGTGATACGCCCCATCGTGAGCGCCACCCAACGTCTGGAAAAAGATATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCAt < 2:664759/150‑1 (MQ=255) gggTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATg > 1:1215126/1‑150 (MQ=255) gggTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATg < 1:3421307/150‑1 (MQ=255) ggTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATga > 1:158342/1‑150 (MQ=255) gTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATgag < 1:4296064/150‑1 (MQ=255) tGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATgaga > 1:294270/1‑150 (MQ=255) ccGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACaaa < 1:767158/150‑1 (MQ=255) ccccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActc > 1:2702604/1‑150 (MQ=255) cccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAActct < 2:2423195/150‑1 (MQ=255) ccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCtt < 1:3667123/150‑1 (MQ=255) ccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCtt < 1:2902627/150‑1 (MQ=255) ccATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCtt > 1:1783416/1‑150 (MQ=255) aTCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTg < 1:4010675/150‑1 (MQ=255) aTCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTg < 1:3059553/150‑1 (MQ=255) aTCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTg < 2:4897505/150‑1 (MQ=255) tCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGc < 2:5042808/150‑1 (MQ=255) cGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCAACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCg > 2:3235220/1‑150 (MQ=255) tGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGAc > 1:187954/1‑150 (MQ=255) gcgcCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGa < 1:2022112/150‑1 (MQ=255) gcgcCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGa < 1:1677310/150‑1 (MQ=255) gcgcCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGa < 1:3144789/150‑1 (MQ=255) ccACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGatat < 1:3327526/150‑1 (MQ=255) cAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGt < 2:2295194/150‑1 (MQ=255) tGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCAttttt > 2:198492/1‑150 (MQ=255) cAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGt > 2:3382679/1‑150 (MQ=255) aaaGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGtt < 2:1054427/150‑1 (MQ=255) aaGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTa < 2:4203772/150‑1 (MQ=255) aaaTACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGca > 1:5031366/1‑150 (MQ=255) aaaTACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGca > 1:929493/1‑150 (MQ=255) aaTACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGCGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGCCGGATATCAGGACATTTTTGCGTTAGcac > 2:1392820/1‑150 (MQ=255) aCAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAgg < 2:2097316/150‑1 (MQ=255) aCAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAgg < 2:3101424/150‑1 (MQ=255) aGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGcc > 1:1264150/1‑150 (MQ=255) cATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTCAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGt > 2:2888395/1‑150 (MQ=255) tCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGa > 1:1941799/1‑150 (MQ=255) cTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAt > 1:2805185/1‑150 (MQ=255) cTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAt < 2:2576589/150‑1 (MQ=255) cTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAt < 1:1566338/150‑1 (MQ=255) cTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAt > 2:4580774/1‑150 (MQ=255) cTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCGTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAt > 2:5105134/1‑150 (MQ=255) tGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAtt < 2:2994889/150‑1 (MQ=255) tGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGAtt > 2:4689355/1‑150 (MQ=255) gCAAAGCGGCGACTGTCGGCTCTCCTGCCTGTCTGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTc < 2:2641136/150‑1 (MQ=255) aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgct > 1:3819846/1‑150 (MQ=255) aGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTctgct > 1:3261538/1‑150 (MQ=255) cggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGa < 2:3754848/150‑1 (MQ=255) ggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGAt < 2:1926632/150‑1 (MQ=255) ggcgACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGAt > 1:2748137/1‑150 (MQ=255) gACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCt < 2:1783416/150‑1 (MQ=255) aCTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTa < 2:2464976/150‑1 (MQ=255) aCTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTa > 2:541349/1‑150 (MQ=255) cTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTaa < 2:1020647/150‑1 (MQ=255) tttCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAc < 1:371601/150‑1 (MQ=255) tttCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAc > 1:3528628/1‑150 (MQ=255) tCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAccc < 1:2363749/150‑1 (MQ=255) cGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcccc > 1:2503931/1‑150 (MQ=255) cGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAAcccc < 2:362359/150‑1 (MQ=255) tctATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACtt > 2:1431145/1‑150 (MQ=255) tATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACtttt > 2:4005288/1‑150 (MQ=255) aTTGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACttttt < 2:3731346/150‑1 (MQ=255) ttGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTa > 1:1281729/1‑150 (MQ=255) tGCCTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTAc > 1:2419011/1‑150 (MQ=255) cTGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACggg < 1:153370/150‑1 (MQ=255) tGTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGt > 1:4784298/1‑150 (MQ=255) gTATGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTa > 2:4987434/1‑150 (MQ=255) aTGTGGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTAtt < 1:2084885/150‑1 (MQ=255) tgGTCGCCGCACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTcc > 2:367315/1‑150 (MQ=255) gccgcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCa > 1:2148174/1‑150 (MQ=255) gccgcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCa > 2:4409407/1‑150 (MQ=255) cgcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGa > 2:572728/1‑150 (MQ=255) gcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAt < 1:2964327/150‑1 (MQ=255) gcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAt > 1:4642378/1‑150 (MQ=255) gcACTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGAt < 1:1367375/150‑1 (MQ=255) aCTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAc > 1:1109740/1‑150 (MQ=255) aCTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAc > 1:4924261/1‑150 (MQ=255) aCTGGTTGTGCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAc > 1:4223537/1‑150 (MQ=255) tgtgCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgcgc > 1:2473198/1‑150 (MQ=255) gtgCGGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATAcgcgcgcg < 1:1726154/150‑1 (MQ=255) tgcggcTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGt > 2:3422559/1‑150 (MQ=255) | GGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTGGCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCTAATCGCTATTGCCGTCTAAGGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGATGCTAACCCCACTTTTTACGGGTATTTCCCGGCAGATACGCGCGCGT > CP009273/2597026‑2597319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |