Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,632,012 C→A 100% intergenic (‑1/+10) bamB ← / ← yfgM BamABCDE complex OM biogenesis lipoprotein/putative anti‑RcsB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,632,0120CA97.5% 848.5 / 8.5 278intergenic (‑1/+10)bamB/yfgMBamABCDE complex OM biogenesis lipoprotein/putative anti‑RcsB factor
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (119/152);  total (122/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 278 total aligned reads displayed.

GCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACGCAGGT  >  CP009273/2631865‑2632158
                                                                                                                                                   |                                                                                                                                                  
 cTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAggg                                                                                                                                                 <  1:5064941/150‑1 (MQ=255)
  tCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGt                                                                                                                                                >  1:588842/1‑150 (MQ=255)
  tCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGt                                                                                                                                                >  2:1652578/1‑150 (MQ=255)
    cACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTcc                                                                                                                                              >  2:150543/1‑150 (MQ=255)
     aCGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTccc                                                                                                                                             <  1:4863291/150‑1 (MQ=255)
     aCGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTccc                                                                                                                                             <  2:1465190/150‑1 (MQ=255)
      cGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCct                                                                                                                                            <  2:648148/150‑1 (MQ=255)
      cGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCct                                                                                                                                            <  1:4323450/150‑1 (MQ=255)
       gCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCctc                                                                                                                                           <  2:4439774/150‑1 (MQ=255)
        ccGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCctct                                                                                                                                          <  1:65021/150‑1 (MQ=255)
         cGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCtctc                                                                                                                                         <  1:1225455/150‑1 (MQ=255)
          gTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCa                                                                                                                                        <  2:3405998/150‑1 (MQ=255)
           tGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAg                                                                                                                                       <  2:4620473/150‑1 (MQ=255)
           tGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAg                                                                                                                                       <  2:3492663/150‑1 (MQ=255)
           tGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAg                                                                                                                                       <  2:3580963/150‑1 (MQ=255)
             gTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGAt                                                                                                                                     >  1:4090148/1‑150 (MQ=255)
             gTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGAt                                                                                                                                     <  1:61342/150‑1 (MQ=255)
              tCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATg                                                                                                                                    <  2:2596183/150‑1 (MQ=255)
                ggCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGa                                                                                                                                  >  2:1714626/1‑150 (MQ=255)
                ggCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGa                                                                                                                                  >  1:1714677/1‑150 (MQ=255)
                 gCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGAc                                                                                                                                 >  1:2103632/1‑150 (MQ=255)
                  cGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACa                                                                                                                                >  1:2714564/1‑150 (MQ=255)
                  cGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACa                                                                                                                                <  1:1799804/150‑1 (MQ=255)
                   gTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACaa                                                                                                                               <  2:3817661/150‑1 (MQ=255)
                   gTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACaa                                                                                                                               >  1:3443751/1‑150 (MQ=255)
                     aaaCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAat                                                                                                                             <  1:3090778/150‑1 (MQ=255)
                     aaaCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAat                                                                                                                             >  1:2082057/1‑150 (MQ=255)
                     aaaCTGGTTTTCAAAGGTTGGCAATGGGGACATCTTTACCACAACTTCTTCGCTGTTAAACAGCGAACAGACTCTTAAAAGGGTAACGGAAATCAGTCCTGGCAGCAGTAATTTACGAAATTGCATAGGGTCACTCTCAGATGGACAAat                                                                                                                             >  1:3639584/1‑150 (MQ=255)
                      aaCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAatt                                                                                                                            >  2:1225713/1‑150 (MQ=255)
                      aaCTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAatt                                                                                                                            >  1:3530370/1‑150 (MQ=255)
                       aCTTGTTTTCAACTGTTGGAAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTATCGGAAAGCAGTCCTGGCAGCAGTAATTTACGCTATTGCATAGGGTCCCTCTCAGATGGACAAatta                                                                                                                           <  2:916390/150‑1 (MQ=255)
                             tttCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAAtt                                                                                                                     >  1:2875571/1‑150 (MQ=255)
                               tCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAAtttt                                                                                                                   <  2:3248819/150‑1 (MQ=255)
                                cAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTc                                                                                                                  <  1:4191078/150‑1 (MQ=255)
                                 aaCGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCANAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCa                                                                                                                 <  1:2678756/150‑1 (MQ=255)
                                    ggTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCt                                                                                                              <  1:1445046/150‑1 (MQ=255)
                                     gTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGACCAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTCCGCACTTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTg                                                                                                             >  2:619152/1‑150 (MQ=255)
                                        ggCAATGGGGACATCTTTACCACATCTTCTTCGCTGTCAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcat                                                                                                          >  1:1248819/1‑150 (MQ=255)
                                           aaTGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGcatcat                                                                                                       >  2:1065385/1‑150 (MQ=255)
                                            aTGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCAtt                                                                                                      <  2:2507745/150‑1 (MQ=255)
                                              ggtgACATCTTTAACACATCTTCTTCGCTTTTAAACAGCGAACAGCCTCTTAAAATGGTAACTGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTATTTTTCATCTGCATCATTTc                                                                                                    <  2:5019158/147‑1 (MQ=255)
                                               gggACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCg                                                                                                   <  2:1280420/150‑1 (MQ=255)
                                                 gACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCt                                                                                                 <  1:2480158/150‑1 (MQ=255)
                                                   cATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTACTTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCa                                                                                               >  2:2690661/1‑150 (MQ=255)
                                                    aTCTTTACAACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAg                                                                                              <  1:1456037/150‑1 (MQ=255)
                                                     tCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGt                                                                                             >  1:1073942/1‑150 (MQ=255)
                                                      cTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTg                                                                                            >  1:4305481/1‑150 (MQ=255)
                                                      cTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTg                                                                                            <  1:4947830/150‑1 (MQ=255)
                                                      cTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTg                                                                                            >  2:3005702/1‑150 (MQ=255)
                                                       tttACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTACTTTTCATCTGCATCAGTTCGCTCAGTGc                                                                                           >  2:2428597/1‑150 (MQ=255)
                                                       tttACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGc                                                                                           >  2:2986371/1‑150 (MQ=255)
                                                         tACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCg                                                                                         >  1:883825/1‑150 (MQ=255)
                                                          aCCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCgg                                                                                        >  1:918751/1‑150 (MQ=255)
                                                               aTCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTaa                                                                                   >  1:4763367/1‑150 (MQ=255)
                                                                tcttctTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAAc                                                                                  >  1:4058136/1‑150 (MQ=255)
                                                                   tcttcGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATc                                                                               <  1:4199375/150‑1 (MQ=255)
                                                                          tGTTAAACAGCGAACAGCCGCTGAAAAGGGTACCGGACAGCAGTCCTGGCAGCAGTCCTTTCCGCACTTGCCTAGGGTCCCTCTCAGATGGACAAATTAGTAATTTTCATCTGCCGCATTTCGCTCAGTGCCGGAGTACCATCGCTTTTc                                                                        >  2:1977606/1‑150 (MQ=255)
                                                                               aaCAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGcc                                                                   >  2:4604307/1‑150 (MQ=255)
                                                                                  aGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCCTAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGc                                                                >  1:4489719/1‑150 (MQ=255)
                                                                                     gAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTc                                                             >  1:861574/1‑150 (MQ=255)
                                                                                      aaCAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTca                                                            <  2:4090148/150‑2 (MQ=255)
                                                                                         aGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCAt                                                         >  2:1780610/1‑150 (MQ=255)
                                                                                           ccGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGc                                                       >  1:1399881/1‑150 (MQ=255)
                                                                                            cGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCa                                                      <  2:4485155/150‑1 (MQ=255)
                                                                                            cGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCa                                                      >  1:1319845/1‑150 (MQ=255)
                                                                                              cTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACt                                                    <  2:3191936/150‑1 (MQ=255)
                                                                                                tAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTAc                                                  >  1:3870773/1‑150 (MQ=255)
                                                                                                tAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTAc                                                  <  1:4030949/150‑1 (MQ=255)
                                                                                                   aaGGGTAACTGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTAcgcg                                               <  2:4305634/150‑1 (MQ=255)
                                                                                                     gggTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCa                                             >  2:4020171/1‑150 (MQ=255)
                                                                                                        tAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCt                                          >  2:2239224/1‑150 (MQ=255)
                                                                                                         aaCGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCtt                                         >  2:4416911/1‑150 (MQ=255)
                                                                                                            ggAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCt                                      >  1:4408613/1‑150 (MQ=255)
                                                                                                             gAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCtt                                     <  1:5161068/150‑1 (MQ=255)
                                                                                                              aaaGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTa                                    >  1:2013507/1‑150 (MQ=255)
                                                                                                              aaaGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTa                                    >  1:2271701/1‑150 (MQ=255)
                                                                                                                aGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATc                                  >  1:508951/1‑150 (MQ=255)
                                                                                                                    gTCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCt                              <  1:1283482/150‑1 (MQ=255)
                                                                                                                     tCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCtt                             <  2:2871068/150‑1 (MQ=255)
                                                                                                                     tCCTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCtt                             <  2:2398594/150‑1 (MQ=255)
                                                                                                                       cTGGCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTATCATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTg                           <  2:4678075/150‑1 (MQ=255)
                                                                                                                         ggCAGCAGTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCt                         >  2:3646199/1‑150 (MQ=255)
                                                                                                                            agcagTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAg                      >  2:2627731/1‑150 (MQ=255)
                                                                                                                            agcagTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAg                      >  2:214910/1‑150 (MQ=255)
                                                                                                                              cagTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCa                    <  1:2740428/150‑1 (MQ=255)
                                                                                                                                gTAATTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAAt                  <  1:5066296/150‑1 (MQ=255)
                                                                                                                                  aaTTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGc                >  1:568786/1‑150 (MQ=255)
                                                                                                                                   aTTTACGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCt               <  1:4675909/150‑1 (MQ=255)
                                                                                                                                        cGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTcacc          >  2:4804857/1‑150 (MQ=255)
                                                                                                                                        cGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTcacc          <  2:565561/150‑1 (MQ=255)
                                                                                                                                        cGCAATTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTcacc          >  2:4804684/1‑150 (MQ=255)
                                                                                                                                         gCAATTGCATAGGGTCCCTCTCAGATGGACAAATGATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTcacca         <  2:1940502/150‑1 (MQ=255)
                                                                                                                                           aaTTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACg       >  2:4910907/1‑150 (MQ=255)
                                                                                                                                           aaTTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACg       <  1:2042759/150‑1 (MQ=255)
                                                                                                                                           aaTTGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACg       >  1:1044320/1‑150 (MQ=255)
                                                                                                                                             ttGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACGCa     >  2:1020412/1‑150 (MQ=255)
                                                                                                                                             ttGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACGCa     <  2:3111005/150‑1 (MQ=255)
                                                                                                                                              tGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACGCAg    >  2:421002/1‑150 (MQ=255)
                                                                                                                                              tGCATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACGCAg    >  2:723926/1‑150 (MQ=255)
                                                                                                                                                cATAGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACGCAGGt  >  2:5128052/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACGGAAAGCAGTCCTGGCAGCAGTAATTTACGCAATTGCATCGGGTCCCTCTCAGATGGACAAATTATTAATTTTCATCTGCATCATTTCGCTCAGTGCCGGAGTAACATCGCTTTTCACGCCTGCTTCCCATGCACTACGCGCACCTTGCTTATCACCTTTGCTCAGCAATGCTTCACCACGCAGGT  >  CP009273/2631865‑2632158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑