Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,665,772 G→T 100% E123* (GAA→TAA)  hcaD → phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,665,7720GT98.4% 774.9 / 1.7 250E123* (GAA→TAA) hcaDphenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (110/135);  total (113/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTG  >  CP009273/2665625‑2665921
                                                                                                                                                   |                                                                                                                                                     
 aTCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAAc                                                                                                                                                    >  1:1661065/1‑150 (MQ=255)
  tCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACg                                                                                                                                                   <  1:2353357/150‑1 (MQ=255)
    tGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgct                                                                                                                                                 <  2:2001905/150‑1 (MQ=255)
      gaTTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCCCTGGGATAACgctgc                                                                                                                                               >  2:699628/2‑150 (MQ=255)
      cATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCTGCCGGTGCTTGATGCACAGGGATAACgctgc                                                                                                                                               >  1:4888751/1‑150 (MQ=255)
       aTTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTGCTTGTTTCACTGGGGTAACgctgcg                                                                                                                                              >  1:627918/1‑149 (MQ=255)
        ttCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCtt                                                                                                                                             <  1:3451387/150‑1 (MQ=255)
        ttCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCtt                                                                                                                                             >  1:5142941/1‑150 (MQ=255)
        ttCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCtt                                                                                                                                             >  1:4303734/1‑150 (MQ=255)
         tCCGGTGTAACCATCAAACCGCTGGGTCGAGACACACGAGAGTTAGTGTTACCCCACGGCGAAAGCTGGCACTGTGATCAGCTTTTTATCGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                            <  2:2974680/150‑1 (MQ=255)
            ggTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGGTGCTTGATGCACAGGGATAAAGCGGCGTTAcc                                                                                                                                         >  1:3043769/1‑150 (MQ=255)
              tgtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCt                                                                                                                                       >  1:667993/1‑150 (MQ=255)
               gtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTa                                                                                                                                      <  2:1798152/150‑1 (MQ=255)
                 aaCCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTTCTTGATGCACTGGGATAAAGCTGCTTTACCCAACg                                                                                                                                    >  1:1635007/1‑150 (MQ=255)
                 aaCCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCGGCTTTACCCTACg                                                                                                                                    >  1:775646/1‑150 (MQ=255)
                      tCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATg                                                                                                                               <  2:2305436/150‑1 (MQ=255)
                      tCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCGTTACCCTACGCCATg                                                                                                                               >  2:4349951/1‑150 (MQ=255)
                       cAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGc                                                                                                                              <  1:280408/150‑1 (MQ=255)
                        aaaaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGcc                                                                                                                             <  1:3375749/150‑1 (MQ=255)
                        aaaaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTAACCGACCCCAAGcc                                                                                                                             >  1:2269929/1‑150 (MQ=255)
                         aaaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCg                                                                                                                            <  1:4796620/150‑1 (MQ=255)
                          aaCGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCgg                                                                                                                           >  2:838478/1‑150 (MQ=255)
                            cGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCGGCCGTTGCTTGATGCACTTGGATAACGCTGCTTTACCCTACGCCATGCCGGTg                                                                                                                         >  1:141199/1‑150 (MQ=255)
                             gCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGa                                                                                                                        <  1:3652980/150‑1 (MQ=255)
                               tGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATg                                                                                                                      <  2:344686/150‑1 (MQ=255)
                               tGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATg                                                                                                                      <  2:5114047/150‑1 (MQ=255)
                                gggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCGTTACCCTACGCCATGCCGGTGATgc                                                                                                                     >  1:1276714/1‑150 (MQ=255)
                                gggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGCTAACGCTGCTTTACCCTACGCCATGCCGGTGATgc                                                                                                                     >  1:274342/1‑150 (MQ=255)
                                 ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc                                                                                                                    <  1:4218858/150‑1 (MQ=255)
                                 ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATgcc                                                                                                                    <  2:1592148/150‑1 (MQ=255)
                                    cgcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGGTTTATGCACGGGGATAAAGCTGCTTTACCCAACGCCCTGCCCGGGATtccgcc                                                                                                                 >  1:3982773/1‑150 (MQ=255)
                                    cgcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATgccgcc                                                                                                                 <  1:1348317/150‑1 (MQ=255)
                                     gcgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCa                                                                                                                <  2:4491993/150‑1 (MQ=255)
                                         cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACt                                                                                                            <  1:442424/150‑1 (MQ=255)
                                         cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACt                                                                                                            <  1:3623217/150‑1 (MQ=255)
                                          acacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTg                                                                                                           >  1:3307819/1‑150 (MQ=255)
                                            acGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGAACGCAGCCGTTGCTTGATGCACTGGGATAACGCTGCATTACCCTACCCCAAGCCGGTGATGCCGCCAGACAGcc                                                                                                         >  2:1362230/1‑149 (MQ=255)
                                               agagTGAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCgaga                                                                                                      <  2:3960653/150‑1 (MQ=255)
                                                 agTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTTCTTTATGCACTGGGATAAAGCTGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAg                                                                                                    >  1:3794879/1‑150 (MQ=255)
                                                  gTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGt                                                                                                   <  1:2148712/150‑1 (MQ=255)
                                                   ttAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGtt                                                                                                  <  2:4445524/150‑1 (MQ=255)
                                                    tAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCGTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTc                                                                                                 >  2:2270182/1‑150 (MQ=255)
                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  2:2424731/150‑1 (MQ=255)
                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  2:2452643/150‑1 (MQ=255)
                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  1:189015/150‑1 (MQ=255)
                                                     aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                                <  1:1352766/150‑1 (MQ=255)
                                                      gtgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTg                                                                                               <  2:1084137/150‑1 (MQ=255)
                                                      gtgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCGGCCGGTGCTTGTTGCACTGGGATAACGCTGCTTTACCCAACCCCAAGCCGGGGCTGCCGCCCGACTGCGAGAAGTTCTg                                                                                               >  1:1828434/1‑150 (MQ=255)
                                                       tgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGc                                                                                              <  2:3608622/150‑1 (MQ=255)
                                                          tAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGc                                                                                           <  1:2618505/150‑1 (MQ=255)
                                                             ccAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCg                                                                                        <  1:1024041/150‑1 (MQ=255)
                                                              cAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGGTTGATTCACTGGGATAACGCAGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCgc                                                                                       >  1:2736785/1‑149 (MQ=255)
                                                              cAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGa                                                                                       >  2:305259/1‑150 (MQ=255)
                                                                 cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACg                                                                                    <  2:5936/150‑1 (MQ=255)
                                                                   gCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGt                                                                                  <  1:2862421/150‑1 (MQ=255)
                                                                    cGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTc                                                                                 >  2:4768678/1‑150 (MQ=255)
                                                                    cGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTc                                                                                 <  2:4747125/150‑1 (MQ=255)
                                                                      aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                               >  2:726803/1‑150 (MQ=255)
                                                                      aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCACCTCGACCGCTGCCGTTGCTTGATGCCAGGGGAAAACGCTGCGTTACCCTACGCCATGCCCGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                               >  2:3168039/1‑150 (MQ=255)
                                                                       aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTTATTCACTGGGATAACGCGGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                              >  1:295322/1‑150 (MQ=255)
                                                                       aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                              >  2:3135717/1‑150 (MQ=255)
                                                                           tGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTTCCGTTGCTTGATGCACTGGGGTAACGCTGCTTTACCCTACGCCATGCCGGTGTTGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTg                                                                          >  1:851666/1‑150 (MQ=255)
                                                                              cACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAtt                                                                       <  2:2700935/150‑1 (MQ=255)
                                                                               aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTa                                                                      <  1:411301/150‑1 (MQ=255)
                                                                               aCTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGTTTCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCCGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTa                                                                      >  2:1680569/1‑150 (MQ=255)
                                                                                cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                     <  1:708595/150‑1 (MQ=255)
                                                                                  gggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGGTGCTTGATGCACAGGGATAACGCTGCTTTAACCAACGCCAAGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCg                                                                   >  1:3405873/1‑150 (MQ=255)
                                                                                   ggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCCGTCGTGATTATCgg                                                                  >  2:5078299/1‑150 (MQ=255)
                                                                                   ggATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCgg                                                                  <  1:4607872/150‑1 (MQ=255)
                                                                                       cAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTAGTGTTTATCGGTGcc                                                              >  2:4143901/1‑150 (MQ=255)
                                                                                              gtctaGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTa                                                       <  2:4672844/147‑1 (MQ=255)
                                                                                               tataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCTTGGCGGTGATGCCGCCAGTATGCGAGATTTTCTGCAGCCCTAACGTTCAGTCGTGTTTATCGGTGCCGGAACTAt                                                      <  2:1519905/150‑1 (MQ=255)
                                                                                                ataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCAAGCCGGTGATTCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATAGGTGCCGGAACTAtt                                                     >  1:2564067/1‑150 (MQ=255)
                                                                                                 taGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTg                                                    <  2:577121/150‑1 (MQ=255)
                                                                                                  aGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTgg                                                   <  1:3260868/150‑1 (MQ=255)
                                                                                                   gCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACGGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGTACTATTGGt                                                  >  2:2761228/1‑150 (MQ=255)
                                                                                                     aaCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCt                                                >  2:3004598/1‑150 (MQ=255)
                                                                                                        cGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGa                                             <  1:1963678/150‑1 (MQ=255)
                                                                                                             cgGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTgg                                        >  2:1074392/1‑150 (MQ=255)
                                                                                                               gCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCt                                      >  1:1230832/1‑150 (MQ=255)
                                                                                                               gCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCt                                      <  2:2513912/150‑1 (MQ=255)
                                                                                                                cAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTg                                     <  2:2718340/150‑1 (MQ=255)
                                                                                                                 aGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGc                                    >  2:918100/1‑150 (MQ=255)
                                                                                                                  gCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGcc                                   <  1:3612590/150‑1 (MQ=255)
                                                                                                                   cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCa                                  <  1:3073214/150‑1 (MQ=255)
                                                                                                                   cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCa                                  >  2:1898708/1‑150 (MQ=255)
                                                                                                                   cTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCa                                  <  2:4912629/150‑1 (MQ=255)
                                                                                                                     cGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAgc                                >  2:2549438/1‑150 (MQ=255)
                                                                                                                         cGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTTCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCa                            <  1:1115572/150‑1 (MQ=255)
                                                                                                                          gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                           >  1:1546505/1‑150 (MQ=255)
                                                                                                                             gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgca                        <  2:1661065/150‑1 (MQ=255)
                                                                                                                              ccGTTGCTTGATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcag                       <  2:2597375/150‑1 (MQ=255)
                                                                                                                                       gATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt              >  2:2609002/1‑150 (MQ=255)
                                                                                                                                       gATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt              >  2:642426/1‑150 (MQ=255)
                                                                                                                                       gATGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGt              >  2:3228386/1‑150 (MQ=255)
                                                                                                                                         tGCACTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTaa            <  2:2842783/150‑1 (MQ=255)
                                                                                                                                            aCTGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGt         >  2:4179059/1‑150 (MQ=255)
                                                                                                                                              tGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGa       >  2:1316876/1‑150 (MQ=255)
                                                                                                                                              tGGGATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGa       >  1:2718844/1‑150 (MQ=255)
                                                                                                                                               gggATAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGAc      >  2:246806/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCCTACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTG  >  CP009273/2665625‑2665921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑