Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,688,006 C→A 100% P299P (CCG→CCT)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,688,0060CA97.2% 923.1 / 11.6 301P299P (CCG→CCT) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base A (137/155);  total (143/158)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 301 total aligned reads displayed.

CGGCTGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTCGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTG  >  CP009273/2687859‑2688152
                                                                                                                                                   |                                                                                                                                                  
cGGCTGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAgg                                                                                                                                                  <  1:922263/150‑1 (MQ=255)
 ggCTGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAggg                                                                                                                                                 <  1:4995948/150‑1 (MQ=255)
   cTGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGggtg                                                                                                                                               >  2:4342942/1‑150 (MQ=255)
    tGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGggtgg                                                                                                                                              <  1:1278131/150‑1 (MQ=255)
    tGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGggtgg                                                                                                                                              <  2:2371475/150‑1 (MQ=255)
      ttCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGtt                                                                                                                                            <  1:3190076/150‑1 (MQ=255)
         gAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTtgtg                                                                                                                                         >  2:1556290/1‑150 (MQ=255)
          aaGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGa                                                                                                                                        <  1:1403665/150‑1 (MQ=255)
          aaGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTAATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCAGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGa                                                                                                                                        <  1:1057765/150‑1 (MQ=255)
             ccAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGtt                                                                                                                                     <  2:1488364/150‑1 (MQ=255)
             ccAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGtt                                                                                                                                     <  2:4031470/150‑1 (MQ=255)
             ccAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGtt                                                                                                                                     <  1:4001970/150‑1 (MQ=255)
               aGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCTCCGTCGGGTGGTTGTGAGTTTc                                                                                                                                   >  1:2055231/1‑150 (MQ=255)
               aGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTc                                                                                                                                   <  1:1763536/150‑1 (MQ=255)
               aGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTc                                                                                                                                   <  1:2807829/150‑1 (MQ=255)
                ggAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCg                                                                                                                                  >  2:3759526/1‑150 (MQ=255)
                ggAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGGGGTTGTGAGTTTCg                                                                                                                                  >  1:1197087/1‑150 (MQ=255)
                  aaTTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGAc                                                                                                                                >  2:1593729/1‑150 (MQ=255)
                   aTTCGCAGGTTGGACACGGAGAAACCAACCAGACCCGCTTTCGGCTGTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACt                                                                                                                               <  2:4955799/150‑1 (MQ=255)
                    ttCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACtt                                                                                                                              <  1:2960053/150‑1 (MQ=255)
                     tCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACttt                                                                                                                             <  1:1666942/150‑1 (MQ=255)
                        cAGGTTGGAAACGGAGAAACCAACCAGACCCTCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTtcat                                                                                                                          <  1:3896794/150‑1 (MQ=255)
                            ttGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGa                                                                                                                      <  1:246609/150‑1 (MQ=255)
                            ttGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGa                                                                                                                      >  2:260953/1‑150 (MQ=255)
                            ttGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGa                                                                                                                      <  1:877219/150‑1 (MQ=255)
                             tGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGaa                                                                                                                     >  1:2371733/1‑150 (MQ=255)
                             tGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGaa                                                                                                                     >  1:2152128/1‑150 (MQ=255)
                                aaaCGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAatat                                                                                                                  >  2:1463585/1‑150 (MQ=255)
                                    ggAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATgcgc                                                                                                              >  1:2701999/1‑150 (MQ=255)
                                     gagaAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGcc                                                                                                             >  1:3527011/1‑150 (MQ=255)
                                     gagaAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGcc                                                                                                             >  1:15800/1‑150 (MQ=255)
                                     gagaAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGcc                                                                                                             <  1:1822779/150‑1 (MQ=255)
                                      agaAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCg                                                                                                            <  2:599605/150‑1 (MQ=255)
                                      agaAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCg                                                                                                            >  1:4215650/1‑150 (MQ=255)
                                            caaccaGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCt                                                                                                      >  1:3836779/1‑150 (MQ=255)
                                            caaccaGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCt                                                                                                      >  2:516647/1‑150 (MQ=255)
                                            caaccaGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCt                                                                                                      <  2:2701999/150‑1 (MQ=255)
                                            caaccaGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCt                                                                                                      >  1:4575235/1‑150 (MQ=255)
                                             aaccaGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTg                                                                                                     <  2:4284083/150‑1 (MQ=255)
                                                 aGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATgg                                                                                                 >  1:3568096/1‑150 (MQ=255)
                                                 aGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATgg                                                                                                 >  2:1019004/1‑150 (MQ=255)
                                                 aGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATgg                                                                                                 <  2:2329958/150‑1 (MQ=255)
                                                  gACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGa                                                                                                >  2:1963765/1‑150 (MQ=255)
                                                     ccGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAAt                                                                                             <  1:4475328/150‑1 (MQ=255)
                                                        cTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGt                                                                                          >  1:1984312/1‑150 (MQ=255)
                                                        cTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGt                                                                                          >  2:4267632/1‑150 (MQ=255)
                                                         tttCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTa                                                                                         <  1:4184000/150‑1 (MQ=255)
                                                         tttCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTa                                                                                         <  2:2767805/150‑1 (MQ=255)
                                                          ttCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAg                                                                                        <  2:22231/150‑1 (MQ=255)
                                                           tCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGc                                                                                       <  2:639028/150‑1 (MQ=255)
                                                           tCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGc                                                                                       >  1:2119015/1‑150 (MQ=255)
                                                            cGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCg                                                                                      <  2:4680781/150‑1 (MQ=255)
                                                                tttGCGCCGCGCCCGGTGGCACCTTAATCGCGGAATTCACCGCCGGAACAGGTCGCCGCGCCCGGCCACGGAGAACTCGCCGTAGGGTGGGTGTGAGTTTCGACTTTCATCAGACTATGCGCCGGTTCCTGATGGAAATCTTCGCGGccc                                                                                  >  2:5071776/1‑150 (MQ=255)
                                                                  tGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGt                                                                                >  2:1072114/1‑150 (MQ=255)
                                                                       cgcgccCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGGCACGGAGAAATCTCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGGCGGTTCCTGTTGGAAATCGTAGCGGCCCGTTTCGt                                                                           <  2:2529378/150‑1 (MQ=255)
                                                                       cgcgccCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGt                                                                           >  1:3120070/1‑150 (MQ=255)
                                                                       cgcgccCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGt                                                                           >  1:4423550/1‑150 (MQ=255)
                                                                             cGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAg                                                                     <  2:511936/150‑1 (MQ=255)
                                                                             cGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAg                                                                     <  1:477114/150‑1 (MQ=255)
                                                                                tGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCaa                                                                  <  2:1188448/150‑1 (MQ=255)
                                                                                     ccTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAg                                                             <  1:5149579/150‑1 (MQ=255)
                                                                                     ccTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAg                                                             <  1:2175613/150‑1 (MQ=255)
                                                                                     ccTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAg                                                             <  2:1280533/150‑1 (MQ=255)
                                                                                     ccTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAg                                                             <  1:3937299/150‑1 (MQ=255)
                                                                                      cTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGc                                                            <  1:2725277/150‑1 (MQ=255)
                                                                                        tCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCgg                                                          <  2:1756968/150‑1 (MQ=255)
                                                                                         cATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGc                                                         >  1:5035352/1‑150 (MQ=255)
                                                                                         cATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGc                                                         <  1:3677591/150‑1 (MQ=255)
                                                                                           tCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGccc                                                       <  2:1080861/150‑1 (MQ=255)
                                                                                                gATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTc                                                  >  2:472869/1‑150 (MQ=255)
                                                                                                 aTTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTCGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGACATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCa                                                 >  2:3053770/1‑150 (MQ=255)
                                                                                                  tttCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAg                                                >  1:1011981/1‑150 (MQ=255)
                                                                                                    tCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGaa                                              <  2:3097112/150‑1 (MQ=255)
                                                                                                     cACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAAc                                             <  1:3705923/150‑1 (MQ=255)
                                                                                                         gccgGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTc                                         >  2:4544240/1‑150 (MQ=255)
                                                                                                         gccgGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTc                                         <  2:1904943/150‑1 (MQ=255)
                                                                                                            ggAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCAt                                      >  1:4324126/1‑150 (MQ=255)
                                                                                                            ggAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCAt                                      >  2:4071419/1‑150 (MQ=255)
                                                                                                              aaCCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTCGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTa                                    <  2:3668644/150‑1 (MQ=255)
                                                                                                                ccGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTAcg                                  <  2:2949566/150‑1 (MQ=255)
                                                                                                                ccGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTAcg                                  <  1:3790227/150‑1 (MQ=255)
                                                                                                                  ggTCGCCGCGCCCGGCCACGGAGAAATCGCCGTCGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTAcggc                                >  1:1567417/1‑150 (MQ=255)
                                                                                                                  ggTCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTAcggc                                <  1:83703/150‑1 (MQ=255)
                                                                                                                    tCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTAcggcgg                              <  1:1310598/150‑1 (MQ=255)
                                                                                                                    tCGCCGCGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTAcggcgg                              >  1:4842846/1‑150 (MQ=255)
                                                                                                                     cgccgcGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACggcggc                             >  2:392530/1‑150 (MQ=255)
                                                                                                                      gccgcGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTTATGGAAATCGTAGCGGCCCGTTTCGTTGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGgcggcg                            <  1:64788/150‑1 (MQ=255)
                                                                                                                      gccgcGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGgcggcg                            <  1:1593729/150‑1 (MQ=255)
                                                                                                                      gccgcGCCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGgcggcg                            <  2:3598978/150‑1 (MQ=255)
                                                                                                                          cgcCCGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTAt                        >  1:3315710/1‑150 (MQ=255)
                                                                                                                            cccGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCt                      >  2:2480418/1‑150 (MQ=255)
                                                                                                                             ccGGCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCtt                     >  2:3808441/1‑150 (MQ=255)
                                                                                                                               ggCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTta                   <  1:4095497/150‑1 (MQ=255)
                                                                                                                                gCCACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTtat                  <  1:1919138/150‑1 (MQ=255)
                                                                                                                                 ccACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTtata                 <  2:838618/150‑1 (MQ=255)
                                                                                                                                  cACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTTATaa                >  2:2106797/1‑150 (MQ=255)
                                                                                                                                  cACGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTTATaa                <  1:2099303/150‑1 (MQ=255)
                                                                                                                                    cGGAGAAATCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTTATAAGc              <  1:4666605/150‑1 (MQ=255)
                                                                                                                                           aTCGCCGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTTATAAGCAgagaga       >  1:3995364/1‑150 (MQ=255)
                                                                                                                                                cGTAGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTg  >  2:375227/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGCTGTTCGAAGCCAGGAATTCGCAGGTTGGAAACGGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGATTTCACCGCCGGAACCGGTCGCCGCGCCCGGCCACGGAGAAATCGCCGTCGGGTGGTTGTGAGTTTCGACTTTCATCAGAATATGCGCCGGTTCCTGATGGAAATCGTAGCGGCCCGTTTCGTGGTCAGCAAAGTAGCGGCCCACTTCAGAACCTTCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTG  >  CP009273/2687859‑2688152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑