Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 236,234 A→T 100% V148E (GTG→GAG)  yafV ← putative NAD(P)‑binding C‑N hydrolase family amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273236,2340AT99.3% 963.8 / ‑2.9 287V148E (GTG→GAG) yafVputative NAD(P)‑binding C‑N hydrolase family amidase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (144/141);  total (145/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 295 total aligned reads displayed.

TCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCG  >  CP009273/236087‑236380
                                                                                                                                                   |                                                                                                                                                  
tCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACcactca                                                                                                                                                  >  1:5088347/1‑150 (MQ=255)
    tGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGaa                                                                                                                                              >  2:5119358/1‑150 (MQ=255)
     gCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGaaa                                                                                                                                             <  1:4635138/150‑1 (MQ=255)
       cGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAAc                                                                                                                                           >  1:2888469/1‑150 (MQ=255)
        gACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACg                                                                                                                                          >  2:844649/1‑150 (MQ=255)
             gATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGt                                                                                                                                     >  1:1908969/1‑150 (MQ=255)
              aTTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTc                                                                                                                                    <  2:2951589/150‑1 (MQ=255)
               ttGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCg                                                                                                                                   >  1:1395690/1‑150 (MQ=255)
               ttGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCg                                                                                                                                   <  2:2562943/150‑1 (MQ=255)
                tGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGt                                                                                                                                  >  2:2480638/1‑150 (MQ=255)
                 gCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTa                                                                                                                                 <  1:3622137/150‑1 (MQ=255)
                  cATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGACAACGTAAGTCGTAg                                                                                                                                >  2:4847351/1‑150 (MQ=255)
                  cATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAg                                                                                                                                <  2:3662198/150‑1 (MQ=255)
                      ccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTCAGTCGTAGcaca                                                                                                                            >  2:4709376/1‑150 (MQ=255)
                          cacaTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCTCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAg                                                                                                                        <  2:2633325/150‑1 (MQ=255)
                          cacaTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAg                                                                                                                        >  1:806729/1‑150 (MQ=255)
                               aCGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCa                                                                                                                   >  1:1024630/1‑150 (MQ=255)
                                cGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCaa                                                                                                                  >  1:1566778/1‑150 (MQ=255)
                                 gCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCaaa                                                                                                                 <  1:3792161/150‑1 (MQ=255)
                                  ccTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCaaaa                                                                                                                >  1:3907304/1‑150 (MQ=255)
                                   cTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                               >  1:2620486/1‑150 (MQ=255)
                                   cTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                               <  2:1812117/150‑1 (MQ=255)
                                       ttCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGc                                                                                                           <  2:502377/150‑1 (MQ=255)
                                        tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGcc                                                                                                          <  2:3466869/150‑1 (MQ=255)
                                          tcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAg                                                                                                        <  2:125970/150‑1 (MQ=255)
                                          tcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAg                                                                                                        >  1:4413840/1‑150 (MQ=255)
                                            aaTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGcc                                                                                                      >  1:2219416/1‑150 (MQ=255)
                                             aTCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCcg                                                                                                     >  1:5018310/1‑150 (MQ=255)
                                              tCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCcgc                                                                                                    <  2:1378717/150‑1 (MQ=255)
                                               cgcgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCcgcg                                                                                                   <  1:4002602/150‑1 (MQ=255)
                                               cgcgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCcgcg                                                                                                   <  1:1728772/150‑1 (MQ=255)
                                               cgcgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCcgcg                                                                                                   >  2:783085/1‑150 (MQ=255)
                                                    gggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCAtt                                                                                              <  1:2422866/150‑1 (MQ=255)
                                                     ggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTc                                                                                             >  2:3770557/1‑150 (MQ=255)
                                                      gCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTcc                                                                                            <  1:4171304/150‑1 (MQ=255)
                                                       ccGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCca                                                                                           <  2:3026110/150‑1 (MQ=255)
                                                       ccGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCca                                                                                           <  2:2188429/150‑1 (MQ=255)
                                                         gTCAGCAATGCCTGCCAGTGCAGAGTGCGCGGAGCAGTCCAGATGGCGACGTACAGGTCGATGACATAATCGTAGATATTGCGCGAACACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAAACGCCAGCCGCGCCATTCcaca                                                                                         >  1:2026252/1‑150 (MQ=255)
                                                           cAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAAt                                                                                       >  2:1144578/1‑150 (MQ=255)
                                                             gCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCa                                                                                     <  2:1547815/150‑1 (MQ=255)
                                                               aaTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACt                                                                                   <  2:2263376/150‑1 (MQ=255)
                                                                 tgcctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGTGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTcg                                                                                 <  2:2704955/150‑1 (MQ=255)
                                                                 tgcctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTcg                                                                                 <  1:4821083/150‑1 (MQ=255)
                                                                   cctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTcgcg                                                                               <  2:660137/150‑1 (MQ=255)
                                                                    ctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCgcgc                                                                              >  1:1865666/1‑150 (MQ=255)
                                                                      gccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCAt                                                                            <  2:171835/150‑1 (MQ=255)
                                                                       ccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCAtt                                                                           <  2:3163298/150‑1 (MQ=255)
                                                                         aGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGc                                                                         <  1:845755/150‑1 (MQ=255)
                                                                           tGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGccc                                                                       <  1:3167006/150‑1 (MQ=255)
                                                                            gCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCg                                                                      >  2:1334192/1‑150 (MQ=255)
                                                                              agagagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                    >  1:897587/1‑150 (MQ=255)
                                                                                agagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCttt                                                                  >  2:53750/1‑150 (MQ=255)
                                                                                 gagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTta                                                                 >  2:3518686/1‑150 (MQ=255)
                                                                                    cgcgGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATaa                                                              <  2:897587/150‑1 (MQ=255)
                                                                                     gcgGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAAt                                                             <  2:751620/150‑1 (MQ=255)
                                                                                      cgGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATg                                                            <  1:2504328/150‑1 (MQ=255)
                                                                                      cgGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATg                                                            <  1:5150729/150‑1 (MQ=255)
                                                                                       ggAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGt                                                           >  2:3989404/1‑150 (MQ=255)
                                                                                        gAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTa                                                          <  2:3701112/150‑1 (MQ=255)
                                                                                         aGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAg                                                         >  2:4191514/1‑150 (MQ=255)
                                                                                          gCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGa                                                        <  2:1662602/150‑1 (MQ=255)
                                                                                          gCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGa                                                        >  1:4989584/1‑150 (MQ=255)
                                                                                           cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGAt                                                       >  2:2512446/1‑150 (MQ=255)
                                                                                           cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGAt                                                       <  1:4431825/150‑1 (MQ=255)
                                                                                           cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGAt                                                       >  2:4073296/1‑150 (MQ=255)
                                                                                              gCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCt                                                    <  2:4404693/150‑1 (MQ=255)
                                                                                              gCCAGTGGGCGACGTACCGGGCGAGGTCATAATCGTGGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCt                                                    <  2:4978936/150‑1 (MQ=255)
                                                                                               ccAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTc                                                   <  1:3703723/150‑1 (MQ=255)
                                                                                                cAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCa                                                  >  1:1309498/1‑150 (MQ=255)
                                                                                                    tGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTg                                              <  2:4528287/150‑1 (MQ=255)
                                                                                                     ggCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGc                                             >  1:2109462/1‑150 (MQ=255)
                                                                                                     ggCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGc                                             >  2:1164248/1‑150 (MQ=255)
                                                                                                        gACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCAt                                          <  2:3380184/150‑1 (MQ=255)
                                                                                                         aCGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATg                                         >  1:2488233/1‑150 (MQ=255)
                                                                                                            tACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCgg                                      >  1:4475859/1‑150 (MQ=255)
                                                                                                             aCAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGa                                     >  2:3928672/1‑150 (MQ=255)
                                                                                                               aGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAAc                                   >  1:2331177/1‑150 (MQ=255)
                                                                                                                 ggCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAg                                 <  1:4293477/150‑1 (MQ=255)
                                                                                                                   cGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                               <  2:3311554/150‑1 (MQ=255)
                                                                                                                       gTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACg                           <  2:1179009/150‑1 (MQ=255)
                                                                                                                       gTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACg                           >  1:4912738/1‑150 (MQ=255)
                                                                                                                         cATAATCGTTGAGATTGCGCGACCACTCATGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCt                         <  2:1492672/150‑1 (MQ=255)
                                                                                                                          aTAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCtt                        >  2:3914802/1‑150 (MQ=255)
                                                                                                                           tAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTa                       <  1:4099903/150‑1 (MQ=255)
                                                                                                                            aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTAt                      <  2:2327340/150‑1 (MQ=255)
                                                                                                                              tCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCa                    <  2:4126553/150‑1 (MQ=255)
                                                                                                                               cGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGACCAGATGACGCTTATCAt                   >  2:3894936/1‑150 (MQ=255)
                                                                                                                               cGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCAt                   <  1:1538591/150‑1 (MQ=255)
                                                                                                                               cGTTGAGAGTGCGCGACCACTCAGGAAAACGTAAGACGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCAt                   <  1:3445760/150‑1 (MQ=255)
                                                                                                                                  tGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaa                >  1:1138915/1‑150 (MQ=255)
                                                                                                                                  tGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGACCAGATGACGCTTATCATaaa                >  2:4682133/1‑150 (MQ=255)
                                                                                                                                   gagaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaa               >  2:2483341/1‑150 (MQ=255)
                                                                                                                                    agaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaaa              >  1:1658802/1‑150 (MQ=255)
                                                                                                                                     gaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAAt             <  2:4787443/150‑1 (MQ=255)
                                                                                                                                      aTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATg            >  1:3495110/1‑150 (MQ=255)
                                                                                                                                          cgcgACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTAcc        <  2:1979480/150‑1 (MQ=255)
                                                                                                                                             gACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTg     >  1:5122980/1‑150 (MQ=255)
                                                                                                                                                cactcaGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTgccg  >  1:4551154/1‑150 (MQ=255)
                                                                                                                                                cactcaGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTgccg  <  2:2793985/150‑1 (MQ=255)
                                                                                                                                                cactcaGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTCTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTgccg  <  1:694987/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGCCG  >  CP009273/236087‑236380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑