Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,712,342 T→A 10.1% G83G (GGT→GGA)  trxC → thioredoxin 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,712,3420TA10.1% 860.4 / 67.4 284G83G (GGT→GGA) trxCthioredoxin 2
Reads supporting (aligned to +/- strand):  ref base T (120/135);  new base A (13/16);  total (133/151)
Fisher's exact test for biased strand distribution p-value = 8.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

GACGGAGAGGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAGCGCCGTTCGATAG  >  CP009273/2712193‑2712491
                                                                                                                                                     |                                                                                                                                                     
gACGGAGAGGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGt                                                                                                                                                       <  1:3574960/150‑1 (MQ=255)
 aCGGAGAGGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTa                                                                                                                                                      <  2:4911721/150‑1 (MQ=255)
  cGGAGAGGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTaa                                                                                                                                                     <  1:4974060/150‑1 (MQ=255)
    gagaGGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAg                                                                                                                                                   <  1:5139297/150‑1 (MQ=255)
     agagGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGt                                                                                                                                                  >  2:665052/1‑150 (MQ=255)
      gagGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTg                                                                                                                                                 <  2:1983689/150‑1 (MQ=255)
           gATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCtt                                                                                                                                            <  1:103884/150‑1 (MQ=255)
            aTTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCTTGGTGCGGCCCCTGCCGTAATATCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCtgt                                                                                                                                           >  1:1772644/1‑148 (MQ=255)
            aTTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCttt                                                                                                                                           >  1:2745791/1‑150 (MQ=255)
            aTTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCttt                                                                                                                                           <  1:4370323/150‑1 (MQ=255)
             ttAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTtg                                                                                                                                          <  2:3428910/150‑1 (MQ=255)
             ttAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTtg                                                                                                                                          <  2:3401508/150‑1 (MQ=255)
             ttAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTtg                                                                                                                                          <  2:1268703/150‑1 (MQ=255)
              tAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGGAGCGGTAAAGTGCGCTTtgt                                                                                                                                         >  1:3230284/1‑150 (MQ=255)
               aaTGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTtgtg                                                                                                                                        <  2:4014867/150‑1 (MQ=255)
                    gACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGt                                                                                                                                   <  2:1158050/150‑1 (MQ=255)
                      ccGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGa                                                                                                                                 <  1:4644358/150‑1 (MQ=255)
                       cGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCNAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGaa                                                                                                                                <  1:2674288/150‑1 (MQ=255)
                       cGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGaa                                                                                                                                >  1:5025205/1‑150 (MQ=255)
                        ggTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAAt                                                                                                                               <  1:2473965/150‑1 (MQ=255)
                            aaaCACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCg                                                                                                                           <  2:1975541/150‑1 (MQ=255)
                               cacTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAg                                                                                                                        >  1:3262935/1‑150 (MQ=255)
                               cacTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAg                                                                                                                        >  2:4831961/1‑150 (MQ=255)
                                acTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGc                                                                                                                       >  2:3642934/1‑150 (MQ=255)
                                  tCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTg                                                                                                                     <  1:4069717/150‑1 (MQ=255)
                                  tCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTg                                                                                                                     <  1:1692824/150‑1 (MQ=255)
                                   cGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGa                                                                                                                    >  2:4721014/1‑150 (MQ=255)
                                    gACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGaa                                                                                                                   <  1:4628580/150‑1 (MQ=255)
                                        aaTTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTg                                                                                                               <  2:2309956/150‑1 (MQ=255)
                                        aaTTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTg                                                                                                               >  1:5078152/1‑150 (MQ=255)
                                           tGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAAt                                                                                                            <  1:2570638/150‑1 (MQ=255)
                                           tGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAAt                                                                                                            >  2:2907930/1‑150 (MQ=255)
                                              tGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACAGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGa                                                                                                         <  2:3890067/150‑1 (MQ=255)
                                                 aGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCa                                                                                                      >  2:5050422/1‑150 (MQ=255)
                                                      gatCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGc                                                                                                 >  2:1107450/1‑150 (MQ=255)
                                                        tCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCtt                                                                                               >  2:4952802/1‑150 (MQ=255)
                                                        tCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCtt                                                                                               >  2:1603930/1‑150 (MQ=255)
                                                         cTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCttt                                                                                              >  2:4339198/1‑150 (MQ=255)
                                                         cTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCttt                                                                                              >  1:5113883/1‑150 (MQ=255)
                                                              tgtgGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAAt                                                                                         >  1:4246188/1‑150 (MQ=255)
                                                              tgtgGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAAt                                                                                         <  2:202358/150‑1 (MQ=255)
                                                              tgtgGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAAt                                                                                         <  1:3283190/150‑1 (MQ=255)
                                                               gtggtgATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAAtt                                                                                        >  1:234648/1‑150 (MQ=255)
                                                               gtggtgATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAAtt                                                                                        <  1:1541039/150‑1 (MQ=255)
                                                               gtggtgATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAAtt                                                                                        <  1:3340793/150‑1 (MQ=255)
                                                               gtggtgATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAAtt                                                                                        >  1:3524556/1‑150 (MQ=255)
                                                                   tgATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCgta                                                                                    <  2:3321929/150‑1 (MQ=255)
                                                                   tgATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCgta                                                                                    >  2:1374797/1‑150 (MQ=255)
                                                                       cGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGtat                                                                                <  1:2886653/150‑1 (MQ=255)
                                                                       cGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGtat                                                                                >  1:911493/1‑150 (MQ=255)
                                                                        gACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGtata                                                                               >  1:4407584/1‑150 (MQ=255)
                                                                        gACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGtata                                                                               <  2:4368878/150‑1 (MQ=255)
                                                                         aCTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATAc                                                                              >  2:2443385/1‑150 (MQ=255)
                                                                          cTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATAcc                                                                             <  1:4833564/150‑1 (MQ=255)
                                                                               gggCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTTAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACcgacga                                                                        <  2:2015440/150‑1 (MQ=255)
                                                                               gggCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACcgacga                                                                        <  1:3534223/150‑1 (MQ=255)
                                                                                ggCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGAt                                                                       >  2:2476223/1‑150 (MQ=255)
                                                                                ggCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGAt                                                                       <  2:1014301/150‑1 (MQ=255)
                                                                                        ggtgCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGAttt                                                               >  2:3283277/1‑150 (MQ=255)
                                                                                         gtgCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCTTGAtttt                                                              <  1:557924/150‑1 (MQ=255)
                                                                                            cGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCaa                                                           <  1:1354287/150‑1 (MQ=255)
                                                                                               ccccTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCaaaaa                                                        >  2:2202410/1‑150 (MQ=255)
                                                                                                 ccTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACg                                                      >  1:826211/1‑150 (MQ=255)
                                                                                                  cTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAATCgg                                                     >  2:444699/1‑150 (MQ=255)
                                                                                                  cTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACgg                                                     <  1:1636804/150‑1 (MQ=255)
                                                                                                     ccGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCa                                                  <  2:3382431/150‑1 (MQ=255)
                                                                                                      cGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAg                                                 >  1:4143265/1‑150 (MQ=255)
                                                                                                          aTTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTg                                             <  2:315765/150‑1 (MQ=255)
                                                                                                            ttCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTc                                           <  1:338463/150‑1 (MQ=255)
                                                                                                             tCGCACCTATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCg                                          <  2:4667767/150‑1 (MQ=255)
                                                                                                              cGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGa                                         >  1:185954/1‑150 (MQ=255)
                                                                                                                 aCCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACAt                                      >  1:2157611/1‑150 (MQ=255)
                                                                                                                 aCCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACAt                                      <  2:4130692/150‑1 (MQ=255)
                                                                                                                  ccAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATg                                     <  2:3796195/150‑1 (MQ=255)
                                                                                                                   cAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGc                                    <  1:1120959/150‑1 (MQ=255)
                                                                                                                    aaTTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCt                                   >  2:2977038/1‑150 (MQ=255)
                                                                                                                    aaTTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCt                                   <  1:4728618/150‑1 (MQ=255)
                                                                                                                     aTTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCtt                                  <  2:1812809/150‑1 (MQ=255)
                                                                                                                        tttGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAAt                               <  2:3563595/150‑1 (MQ=255)
                                                                                                                        tttGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAAt                               <  1:2036563/150‑1 (MQ=255)
                                                                                                                          tGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATgg                             <  1:3809621/150‑1 (MQ=255)
                                                                                                                          tGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATgg                             <  1:3664914/150‑1 (MQ=255)
                                                                                                                           gAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGgc                            >  2:3552017/1‑150 (MQ=255)
                                                                                                                              gATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCa                         >  2:2686718/1‑150 (MQ=255)
                                                                                                                              gATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCa                         <  1:1409287/150‑1 (MQ=255)
                                                                                                                                tGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGt                       >  2:1584954/1‑150 (MQ=255)
                                                                                                                                 gTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTa                      >  2:171014/1‑150 (MQ=255)
                                                                                                                                   cgcgCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTAcc                    <  2:5152764/150‑1 (MQ=255)
                                                                                                                                    gcgcAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGACGCTGAACGTGACTTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCg                   <  2:4862282/150‑1 (MQ=255)
                                                                                                                                       cAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGaaa                <  2:3049342/150‑1 (MQ=255)
                                                                                                                                       cAAGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGaaa                >  1:2422565/1‑150 (MQ=255)
                                                                                                                                        aaGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAg               >  2:4081515/1‑150 (MQ=255)
                                                                                                                                        aaGAGCGTAGCGGAAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACAGAAAg               <  1:3019473/150‑1 (MQ=255)
                                                                                                                                          gagCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAgcg             >  2:4893596/1‑150 (MQ=255)
                                                                                                                                            gCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAGCGcc           <  1:3642934/150‑1 (MQ=255)
                                                                                                                                              gTAGAGGTATAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAGCGCCGt         <  2:5076637/150‑1 (MQ=255)
                                                                                                                                               tAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAGCGCCGtt        <  2:2573820/150‑1 (MQ=255)
                                                                                                                                                aGCGGTAAAGTGCGCTTTGTGAATGTGATTACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGATTAATGGCGCAGTACCGAAAGCGCCGTTc       <  1:2088542/150‑1 (MQ=255)
                                                                                                                                                    gTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAGCGCCGTTCGATa   <  1:4244163/150‑1 (MQ=255)
                                                                                                                                                     tAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCGGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAGCGCCGTTCGATAg  >  2:2144549/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACGGAGAGGTGATTAATGCGACCGGTGAAACACTCGACAAATTGCTGAAGGATGATCTACCTGTGGTGATCGACTTCTGGGCACCGTGGTGCGGCCCCTGCCGTAATTTCGCACCAATTTTTGAAGATGTCGCGCAAGAGCGTAGCGGTAAAGTGCGCTTTGTGAAAGTGAATACCGAAGCTGAACGTGAATTGAGCAGTCGCTTTGGAATTCGTAGTATACCGACGATCATGATTTTCAAAAACGGTCAGGTTGTCGACATGCTTAATGGCGCAGTACCGAAAGCGCCGTTCGATAG  >  CP009273/2712193‑2712491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑