Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,808,907 C→A 100% E298* (GAA→TAA)  gshA ← glutamate‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,808,9070CA98.9% 926.5 / 0.6 283E298* (GAA→TAA) gshAglutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (138/141);  total (140/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 284 total aligned reads displayed.

CCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAA  >  CP009273/2808760‑2809053
                                                                                                                                                   |                                                                                                                                                  
 ctgctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTCCagttagtt                                                                                                                                                 >  2:4635575/1‑150 (MQ=255)
   gctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGtttt                                                                                                                                               >  1:4453474/1‑150 (MQ=255)
   gctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGtttt                                                                                                                                               <  2:489492/150‑1 (MQ=255)
   gctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGtttt                                                                                                                                               <  2:3592152/150‑1 (MQ=255)
       ttCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAAt                                                                                                                                           >  2:1029918/1‑150 (MQ=255)
        tCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATc                                                                                                                                          <  2:3339132/150‑1 (MQ=255)
        tCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATc                                                                                                                                          >  2:2000446/1‑150 (MQ=255)
         cATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCt                                                                                                                                         >  2:3712268/1‑150 (MQ=255)
          aTCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTg                                                                                                                                        >  1:2316652/1‑150 (MQ=255)
             tACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCaa                                                                                                                                     >  1:2325125/1‑150 (MQ=255)
                   aaTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGtt                                                                                                                               <  1:3807752/150‑1 (MQ=255)
                   aaTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGtt                                                                                                                               <  2:727691/150‑1 (MQ=255)
                    aTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTg                                                                                                                              <  1:984472/150‑1 (MQ=255)
                      cGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCt                                                                                                                            <  1:1648307/150‑1 (MQ=255)
                      cGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCt                                                                                                                            <  1:3902372/150‑1 (MQ=255)
                       ggCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTg                                                                                                                           >  2:1495401/1‑150 (MQ=255)
                       ggCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTg                                                                                                                           <  1:195619/150‑1 (MQ=255)
                         cGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGtt                                                                                                                         <  1:1123944/150‑1 (MQ=255)
                         cGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGtt                                                                                                                         <  1:2434567/150‑1 (MQ=255)
                          gagaACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTg                                                                                                                        <  2:1902004/150‑1 (MQ=255)
                          gagaACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTg                                                                                                                        >  2:1688052/1‑150 (MQ=255)
                                gggTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGc                                                                                                                  <  1:233986/150‑1 (MQ=255)
                                 ggTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCa                                                                                                                 <  2:4383202/150‑1 (MQ=255)
                                 ggTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCa                                                                                                                 <  1:2210126/150‑1 (MQ=255)
                                     gATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCct                                                                                                             >  1:249975/1‑150 (MQ=255)
                                       tGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCctca                                                                                                           <  1:13154/150‑2 (MQ=255)
                                       tGTCCAGCGAACGCACTTCAATATAGTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCctct                                                                                                           <  2:2092017/150‑1 (MQ=255)
                                          ccAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTAc                                                                                                        >  2:1046366/1‑150 (MQ=255)
                                              cGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc                                                                                                    >  2:4216714/1‑150 (MQ=255)
                                               gAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCt                                                                                                   >  2:5028788/1‑150 (MQ=255)
                                               gAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCt                                                                                                   <  2:1168250/150‑1 (MQ=255)
                                                aaCGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCtt                                                                                                  <  2:2265561/150‑1 (MQ=255)
                                                   gCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtct                                                                                               <  1:3667426/150‑1 (MQ=255)
                                                    cACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtctc                                                                                              >  2:1340381/1‑150 (MQ=255)
                                                     aCTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCa                                                                                             <  1:2015453/150‑1 (MQ=255)
                                                       ttCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAAt                                                                                           >  1:4334124/1‑150 (MQ=255)
                                                       ttCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAAt                                                                                           <  1:2797928/150‑1 (MQ=255)
                                                        tCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATa                                                                                          <  1:4722364/150‑1 (MQ=255)
                                                           atatatTCACTGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCa                                                                                       <  2:3228396/150‑1 (MQ=255)
                                                           atatatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCa                                                                                       <  2:3097163/150‑1 (MQ=255)
                                                                ttCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCtt                                                                                  <  1:2972594/150‑1 (MQ=255)
                                                                ttCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTAGCCGCTGAGGGTAAAGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGACTCTTACCGTCTTTCTCAATACCAATCtt                                                                                  <  2:1545886/150‑1 (MQ=255)
                                                                 tCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTACCGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTc                                                                                 >  2:1394546/1‑150 (MQ=255)
                                                                  cAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTGcg                                                                                >  2:3417881/1‑150 (MQ=255)
                                                                      gccgccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTa                                                                            <  2:3683070/150‑1 (MQ=255)
                                                                       ccgccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTAc                                                                           >  1:728320/1‑150 (MQ=255)
                                                                         gccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTActc                                                                         >  1:1868857/1‑150 (MQ=255)
                                                                           cACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCtt                                                                       >  2:2874352/1‑150 (MQ=255)
                                                                              gTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCg                                                                    <  2:1648068/150‑1 (MQ=255)
                                                                              gTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCg                                                                    >  1:4274915/1‑150 (MQ=255)
                                                                              gTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCg                                                                    >  1:3190408/1‑150 (MQ=255)
                                                                               tAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGTAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGa                                                                   <  2:1271220/150‑1 (MQ=255)
                                                                                 aCAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATg                                                                 <  2:2379341/150‑1 (MQ=255)
                                                                                   aGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGc                                                               >  1:928853/1‑150 (MQ=255)
                                                                                   aGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGc                                                               >  1:4190014/1‑150 (MQ=255)
                                                                                   aGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGc                                                               <  1:2900197/150‑1 (MQ=255)
                                                                                    gcgcATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCg                                                              >  1:1903106/1‑150 (MQ=255)
                                                                                      gcATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGtt                                                            >  1:2763345/1‑150 (MQ=255)
                                                                                       cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGttt                                                           <  2:2458881/150‑1 (MQ=255)
                                                                                       cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGttt                                                           >  2:4308010/1‑150 (MQ=255)
                                                                                        aTCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGtttt                                                          <  1:3332133/150‑1 (MQ=255)
                                                                                         tCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTGTTg                                                         >  2:2656850/1‑150 (MQ=255)
                                                                                           aGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAt                                                       >  1:4822582/1‑150 (MQ=255)
                                                                                           aGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAt                                                       >  2:522830/1‑150 (MQ=255)
                                                                                            gAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAtt                                                      >  2:911309/1‑150 (MQ=255)
                                                                                            gAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAtt                                                      >  1:2597953/1‑150 (MQ=255)
                                                                                             aaGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTg                                                     <  1:4918250/150‑1 (MQ=255)
                                                                                                  gACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                                >  1:1982909/1‑150 (MQ=255)
                                                                                                  gACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTACAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                                >  1:3805107/1‑150 (MQ=255)
                                                                                                    cTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGttt                                              <  1:3106439/150‑1 (MQ=255)
                                                                                                     tCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTa                                             <  2:388029/150‑1 (MQ=255)
                                                                                                      cgccgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTaa                                            <  2:4453474/150‑1 (MQ=255)
                                                                                                            tGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCg                                      <  1:4762468/150‑1 (MQ=255)
                                                                                                              cGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCt                                    <  1:456954/150‑1 (MQ=255)
                                                                                                               gggTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTa                                   >  1:591856/1‑150 (MQ=255)
                                                                                                                ggTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTAc                                  >  1:4969432/1‑150 (MQ=255)
                                                                                                                 gTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACg                                 <  2:2745728/150‑1 (MQ=255)
                                                                                                                 gTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACg                                 <  1:1219371/150‑1 (MQ=255)
                                                                                                                   aaCGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTa                               <  2:5124571/150‑1 (MQ=255)
                                                                                                                    aCGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATTTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTAc                              <  2:4772249/150‑1 (MQ=255)
                                                                                                                     cgcgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACt                             >  2:1835854/1‑150 (MQ=255)
                                                                                                                       cgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCg                           >  1:2779930/1‑150 (MQ=255)
                                                                                                                        gTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGt                          >  2:584721/1‑150 (MQ=255)
                                                                                                                             ggACGAATCGGCGCGTACAGTTAGTTGTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGa                     <  2:4787045/150‑1 (MQ=255)
                                                                                                                               aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATc                   >  1:4619200/1‑150 (MQ=255)
                                                                                                                               aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATc                   <  2:4586352/150‑1 (MQ=255)
                                                                                                                                 gAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGt                 <  2:4691597/150‑1 (MQ=255)
                                                                                                                                  aaTCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGtt                <  1:3712268/150‑1 (MQ=255)
                                                                                                                                   aTCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTg               >  2:2971507/1‑150 (MQ=255)
                                                                                                                                    tCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGa              >  2:4503686/1‑150 (MQ=255)
                                                                                                                                     cGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGaa             >  1:3220283/1‑150 (MQ=255)
                                                                                                                                     cGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGaa             >  1:633093/1‑150 (MQ=255)
                                                                                                                                      ggCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAg            >  1:2972978/1‑150 (MQ=255)
                                                                                                                                         gcgTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTa         <  1:109000/150‑1 (MQ=255)
                                                                                                                                           gTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAt       <  1:5152375/150‑1 (MQ=255)
                                                                                                                                           gTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAt       <  1:1591705/150‑1 (MQ=255)
                                                                                                                                            tACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATa      <  2:3802612/150‑1 (MQ=255)
                                                                                                                                             aCAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATAc     >  2:39362/1‑150 (MQ=255)
                                                                                                                                              cAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATAcc    >  2:2608599/1‑150 (MQ=255)
                                                                                                                                                gTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCaa  <  2:314059/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAA  >  CP009273/2808760‑2809053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑