Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,841,044 C→A 100% A519A (GCG→GCT)  hycC ← hydrogenase 3, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,841,0440CA100.0% 1216.3 / NA 360A519A (GCG→GCT) hycChydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (197/163);  total (197/163)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 360 total aligned reads displayed.

TTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAAT  >  CP009273/2840898‑2841190
                                                                                                                                                  |                                                                                                                                                  
ttGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGcc                                                                                                                                                 >  2:834796/1‑150 (MQ=255)
ttGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGcc                                                                                                                                                 <  1:4336118/150‑1 (MQ=255)
      aGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatg                                                                                                                                           <  2:1329999/150‑1 (MQ=255)
       gTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatga                                                                                                                                          >  2:68936/1‑150 (MQ=255)
        tttCAGCACCGTCGCAAACGGCTGTTTCACCGGCATGGCAATACAGTGAGCTGTAATCACCATTTATTTTTCGTGGTCGTTACGGCACACCCATGCCGCACCGCGGGTTCGCGATGGCAAACGATCGCCTTTGCAAATAGCCATTatgat                                                                                                                                         <  1:3169340/150‑1 (MQ=255)
         ttCAGCACCGGCGCAAACGCCTGTTTCACCGGCTTGGCATAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAatgatg                                                                                                                                        <  1:2583632/150‑1 (MQ=255)
          tCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAAtgatga                                                                                                                                       >  1:167528/1‑150 (MQ=255)
           cAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGaa                                                                                                                                      <  1:1063424/150‑1 (MQ=255)
           cAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGaa                                                                                                                                      >  1:4186300/1‑150 (MQ=255)
             gCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATg                                                                                                                                    <  1:1446002/150‑1 (MQ=255)
               aCCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgc                                                                                                                                  >  1:3470442/1‑150 (MQ=255)
                ccGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgca                                                                                                                                 >  1:4383110/1‑150 (MQ=255)
                  ggCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGgcagc                                                                                                                               >  2:4259441/1‑150 (MQ=255)
                    cgcAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGcagcag                                                                                                                             <  2:1156185/150‑1 (MQ=255)
                       aaaCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCgg                                                                                                                          <  2:5104871/150‑1 (MQ=255)
                        aaCGCCTGTTTCACCGGCATGGTAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCggg                                                                                                                         >  2:3408768/1‑150 (MQ=255)
                         aCGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggc                                                                                                                        >  2:4762956/1‑150 (MQ=255)
                          cGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggca                                                                                                                       >  1:5036972/1‑150 (MQ=255)
                           gCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcag                                                                                                                      >  1:801555/1‑150 (MQ=255)
                           gCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcag                                                                                                                      >  2:4521593/1‑150 (MQ=255)
                            ccTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATAACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcagg                                                                                                                     >  1:2529324/1‑150 (MQ=255)
                             cTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggc                                                                                                                    >  1:2739422/1‑150 (MQ=255)
                              tGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGggcaggca                                                                                                                   <  2:5045975/150‑1 (MQ=255)
                               gTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCaa                                                                                                                  >  2:2743386/1‑150 (MQ=255)
                               gTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCaa                                                                                                                  >  2:1809288/1‑150 (MQ=255)
                               gTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCaa                                                                                                                  >  2:3975045/1‑150 (MQ=255)
                                tttCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAAt                                                                                                                 >  1:2683705/1‑150 (MQ=255)
                                 ttCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATc                                                                                                                >  2:3711185/1‑150 (MQ=255)
                                  tCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATca                                                                                                               >  1:599794/1‑150 (MQ=255)
                                   cACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                              <  2:2306470/150‑1 (MQ=255)
                                   cACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                              >  2:3672929/1‑150 (MQ=255)
                                   cACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATcag                                                                                                              >  2:1609753/1‑150 (MQ=255)
                                       ggCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCagcagc                                                                                                          >  2:154417/1‑150 (MQ=255)
                                       ggCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCagcagc                                                                                                          <  1:973608/150‑1 (MQ=255)
                                             gCAAAACCGTGAGCGGTAATCACCATGGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTg                                                                                                    <  2:1490168/150‑1 (MQ=255)
                                               aaaaCCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGat                                                                                                  <  2:5010243/150‑1 (MQ=255)
                                                        aGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTg                                                                                         >  2:1408150/1‑150 (MQ=255)
                                                        aGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTg                                                                                         <  1:635232/150‑1 (MQ=255)
                                                          cGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTgag                                                                                       <  2:172531/150‑1 (MQ=255)
                                                          cGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTgag                                                                                       <  2:515255/150‑1 (MQ=255)
                                                              aaTCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAAc                                                                                   >  1:2030607/1‑150 (MQ=255)
                                                                 cACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggt                                                                                >  1:4657945/1‑150 (MQ=255)
                                                                  aCCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACggtg                                                                               <  1:731421/150‑1 (MQ=255)
                                                                      ttGATTTTTCGTGGTCGTACCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGgtgt                                                                           <  2:2714585/150‑1 (MQ=255)
                                                                       tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGtt                                                                          <  2:342935/150‑1 (MQ=255)
                                                                        gATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTa                                                                         <  1:2343090/150‑1 (MQ=255)
                                                                          tttttAGTTGTAGTTACCGCACACCCAGGACGCACAGCGGTAACGCGATTGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAga                                                                       <  2:2613612/150‑2 (MQ=255)
                                                                           ttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCACATAGCCATACTGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCCGCGGTGGTGACACGGTGGTGTGAGCa                                                                      >  2:2962849/1‑150 (MQ=255)
                                                                                    tCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGc                                                             <  1:2984843/150‑1 (MQ=255)
                                                                                    tCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGc                                                             <  1:4502735/150‑1 (MQ=255)
                                                                                    tCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGc                                                             <  2:1187834/150‑1 (MQ=255)
                                                                                     cGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCg                                                            >  2:4740441/1‑150 (MQ=255)
                                                                                      gTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGc                                                             <  1:3796163/148‑1 (MQ=255)
                                                                                      gTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCgg                                                           >  1:2474536/1‑150 (MQ=255)
                                                                                      gTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCgg                                                           >  2:4543917/1‑150 (MQ=255)
                                                                                      gTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCgg                                                           >  1:1807740/1‑150 (MQ=255)
                                                                                        aaCCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCACCGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCa                                                         >  2:2197450/1‑150 (MQ=255)
                                                                                          ccGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCaga                                                       <  1:4018191/150‑1 (MQ=255)
                                                                                           cGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCagag                                                      >  2:3470669/1‑150 (MQ=255)
                                                                                            gCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAgg                                                     >  1:4101522/1‑150 (MQ=255)
                                                                                             cacaCCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGt                                                    <  1:4420098/150‑1 (MQ=255)
                                                                                             cacaCCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGt                                                    <  1:1828122/150‑1 (MQ=255)
                                                                                              acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                   <  2:211326/150‑1 (MQ=255)
                                                                                              acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTa                                                   <  2:2076170/150‑1 (MQ=255)
                                                                                               cacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAc                                                  >  1:3939015/1‑150 (MQ=255)
                                                                                                acCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAca                                                 <  1:1122823/150‑1 (MQ=255)
                                                                                                    aGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcag                                             >  2:3278107/1‑150 (MQ=255)
                                                                                                     ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagc                                            >  2:2016920/1‑150 (MQ=255)
                                                                                                     ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagc                                            >  2:4506825/1‑150 (MQ=255)
                                                                                                     ggCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagc                                            >  2:3224498/1‑150 (MQ=255)
                                                                                                      gCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagca                                           <  1:1257011/150‑1 (MQ=255)
                                                                                                      gCCGCACCGCGGGAACGCGAGGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagca                                           <  2:1863893/150‑1 (MQ=255)
                                                                                                          cACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagaga                                       <  1:4945894/150‑1 (MQ=255)
                                                                                                          cACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagaga                                       >  1:363323/1‑150 (MQ=255)
                                                                                                               cgGGAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATc                                  <  2:606024/150‑1 (MQ=255)
                                                                                                                gggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                                 >  2:2513954/1‑150 (MQ=255)
                                                                                                                 ggAACGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCgg                                >  1:874432/1‑150 (MQ=255)
                                                                                                                    aCGCGATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAg                             <  1:3975045/150‑1 (MQ=255)
                                                                                                                     cgcgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGt                            <  1:154417/150‑1 (MQ=255)
                                                                                                                     cgcgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGt                            <  2:3777715/150‑1 (MQ=255)
                                                                                                                      gcgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTa                           <  2:2264383/150‑1 (MQ=255)
                                                                                                                       cgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAg                          <  2:2847821/150‑1 (MQ=255)
                                                                                                                       cgATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAg                          >  1:306141/1‑150 (MQ=255)
                                                                                                                        gATGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGc                         <  1:882555/150‑1 (MQ=255)
                                                                                                                          tGGCAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGTCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCa                       <  1:2142703/150‑1 (MQ=255)
                                                                                                                             cAAACGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACgg                    >  1:4990575/1‑150 (MQ=255)
                                                                                                                             cAAACGATCGCCTGTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACgg                    <  2:669856/150‑1 (MQ=255)
                                                                                                                              aaaCGATCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGgc                   <  2:4186300/150‑1 (MQ=255)
                                                                                                                                    tCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagca             <  1:1561803/150‑1 (MQ=255)
                                                                                                                                    tCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagca             >  2:1950344/1‑150 (MQ=255)
                                                                                                                                    tCGCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcagca             >  1:4815374/1‑150 (MQ=255)
                                                                                                                                      gCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac           <  1:2391836/150‑1 (MQ=255)
                                                                                                                                      gCCTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAac           <  2:2798170/150‑1 (MQ=255)
                                                                                                                                       ccTTTGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAaca          <  1:1023790/150‑1 (MQ=255)
                                                                                                                                         tttGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACAcc        <  1:2035410/150‑1 (MQ=255)
                                                                                                                                           tGCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc      >  1:5112882/1‑150 (MQ=255)
                                                                                                                                            gCAAATAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGcc     <  1:2204083/150‑1 (MQ=255)
                                                                                                                                              aaaTAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCaa   >  2:381658/1‑150 (MQ=255)
                                                                                                                                              aaaTAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCaa   >  2:2821481/1‑150 (MQ=255)
                                                                                                                                              aaaTAGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCaa   >  2:127826/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTGCGTAGTTTCAGCACCGGCGCAAACGCCTGTTTCACCGGCATGGCAAAACCGTGAGCGGTAATCACCATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAAT  >  CP009273/2840898‑2841190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑