Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,869,780 G→A 100% intergenic (‑92/‑160) cysD ← / → iap sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,869,7800GA99.2% 799.2 / ‑2.8 243intergenic (‑92/‑160)cysD/iapsulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (114/127);  total (116/127)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 243 total aligned reads displayed.

ATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATT  >  CP009273/2869633‑2869927
                                                                                                                                                   |                                                                                                                                                   
atGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAag                                                                                                                                                   >  1:2053107/1‑150 (MQ=255)
  gtgGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAagta                                                                                                                                                 <  2:1451901/150‑1 (MQ=255)
        gCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCt                                                                                                                                           >  1:4885251/1‑150 (MQ=255)
         cTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCtt                                                                                                                                          >  1:3432210/1‑150 (MQ=255)
           ttCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTaa                                                                                                                                        <  2:4368128/150‑1 (MQ=255)
              cGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACgg                                                                                                                                     <  2:4029229/150‑1 (MQ=255)
              cGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACgg                                                                                                                                     >  1:3190712/1‑150 (MQ=255)
                ccTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACggga                                                                                                                                   <  1:2370899/150‑3 (MQ=255)
                 cTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAAt                                                                                                                                  >  2:1601940/1‑150 (MQ=255)
                 cTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAAt                                                                                                                                  <  2:3818500/150‑1 (MQ=255)
                   ccAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATaa                                                                                                                                >  2:2066247/1‑150 (MQ=255)
                     aGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACg                                                                                                                              <  2:2837241/150‑1 (MQ=255)
                        tGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGAtt                                                                                                                           >  1:4647409/1‑150 (MQ=255)
                         gCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGAttt                                                                                                                          >  2:2980630/1‑150 (MQ=255)
                         gCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGNTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGAttt                                                                                                                          >  1:2680769/1‑150 (MQ=255)
                          ccGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTg                                                                                                                         <  2:21221/150‑1 (MQ=255)
                           cGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTgg                                                                                                                        <  1:2358767/150‑1 (MQ=255)
                              aGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCaa                                                                                                                     <  2:2221286/150‑1 (MQ=255)
                                 tGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGc                                                                                                                  <  2:1156602/150‑1 (MQ=255)
                                   aGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTa                                                                                                                <  1:5050812/150‑1 (MQ=255)
                                     tAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAat                                                                                                              <  1:408113/150‑1 (MQ=255)
                                     tAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAat                                                                                                              <  1:3989052/150‑1 (MQ=255)
                                      aaGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAata                                                                                                             <  1:703005/150‑1 (MQ=255)
                                       aGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAatat                                                                                                            <  2:1804403/150‑1 (MQ=255)
                                       aGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAatat                                                                                                            <  1:2504392/150‑1 (MQ=255)
                                        gTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATc                                                                                                           >  1:4197070/1‑150 (MQ=255)
                                          cGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaa                                                                                                         >  1:1590059/1‑150 (MQ=255)
                                            tATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCaaaa                                                                                                       >  2:4840490/1‑150 (MQ=255)
                                              tttGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAg                                                                                                     >  1:2764267/1‑150 (MQ=255)
                                               ttGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGt                                                                                                    <  1:2031278/150‑1 (MQ=255)
                                                 gATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGc                                                                                                  <  1:3531440/150‑1 (MQ=255)
                                                    ccATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTa                                                                                               <  1:2068083/150‑1 (MQ=255)
                                                     cATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAAGAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTCAGATCCAAAAGTGCTTaa                                                                                              >  2:2658055/1‑150 (MQ=255)
                                                      aTAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                                                                             <  2:1400153/150‑1 (MQ=255)
                                                      aTAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAAGTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAg                                                                                             <  2:530901/150‑1 (MQ=255)
                                                        aaCCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGc                                                                                           >  2:1345873/1‑150 (MQ=255)
                                                        aaCCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCCTAAGGc                                                                                           >  2:4736662/1‑150 (MQ=255)
                                                         aCCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCa                                                                                          >  1:3232358/1‑150 (MQ=255)
                                                            gTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCg                                                                                       <  1:1804957/150‑1 (MQ=255)
                                                            gTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGGTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCg                                                                                       <  1:482744/150‑1 (MQ=255)
                                                             ttCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCgg                                                                                      <  1:3742274/150‑1 (MQ=255)
                                                             ttCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCgg                                                                                      <  1:1907111/150‑1 (MQ=255)
                                                              tCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGa                                                                                     <  1:1086838/150‑1 (MQ=255)
                                                               ccTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGAt                                                                                    <  1:4420189/150‑1 (MQ=255)
                                                               ccTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGAt                                                                                    <  2:2506687/150‑1 (MQ=255)
                                                                 tttGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGAttt                                                                                  <  1:2995811/150‑1 (MQ=255)
                                                                  ttGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTc                                                                                 <  1:2245835/150‑1 (MQ=255)
                                                                   tGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCg                                                                                <  2:4883035/150‑1 (MQ=255)
                                                                   tGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCg                                                                                <  1:802595/150‑1 (MQ=255)
                                                                    gCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCgg                                                                               >  1:4609533/1‑150 (MQ=255)
                                                                      taTACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGc                                                                             >  1:1034022/2‑150 (MQ=255)
                                                                      aaTACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGc                                                                             <  2:4450011/150‑1 (MQ=255)
                                                                         aCCGATATTTTCTTTCAATCAGATTTTTCGACTATAGGGAGCGTAAGAGATCGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGtt                                                                          <  2:75968/150‑1 (MQ=255)
                                                                          ccGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGttt                                                                         >  1:1377270/1‑150 (MQ=255)
                                                                          ccGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGttt                                                                         <  2:2556965/150‑1 (MQ=255)
                                                                           cGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTa                                                                        <  2:1265394/150‑1 (MQ=255)
                                                                             cTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAgg                                                                      >  1:356813/1‑150 (MQ=255)
                                                                              tATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGa                                                                     >  2:884572/1‑150 (MQ=255)
                                                                                ttttCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAg                                                                   <  1:563960/150‑1 (MQ=255)
                                                                                      tGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGa                                                             >  1:1117963/1‑150 (MQ=255)
                                                                                         cATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAt                                                          >  2:1159484/1‑150 (MQ=255)
                                                                                          aTCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAtt                                                         <  1:1468707/150‑1 (MQ=255)
                                                                                          aTCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAtt                                                         >  1:2573117/1‑150 (MQ=255)
                                                                                            cAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGt                                                       >  1:1231837/1‑150 (MQ=255)
                                                                                              gATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGttt                                                     <  2:3232358/150‑1 (MQ=255)
                                                                                               aTGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGtttt                                                    >  1:4083086/1‑150 (MQ=255)
                                                                                                    tCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAgcgc                                               >  2:3317475/1‑150 (MQ=255)
                                                                                                     cGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCa                                              <  1:2209208/150‑1 (MQ=255)
                                                                                                      gACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAg                                             <  2:4606280/150‑1 (MQ=255)
                                                                                                        cTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCg                                           >  2:424553/1‑150 (MQ=255)
                                                                                                        cTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCg                                           >  2:1901762/1‑150 (MQ=255)
                                                                                                            aGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGGATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAg                                       >  1:4352295/1‑150 (MQ=255)
                                                                                                              ggAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGtt                                     <  1:1829330/150‑1 (MQ=255)
                                                                                                               gAGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGttt                                    >  2:4775326/1‑150 (MQ=255)
                                                                                                                aGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTc                                   >  1:2632177/1‑150 (MQ=255)
                                                                                                                aGCGTAAGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTc                                   <  1:3029429/150‑1 (MQ=255)
                                                                                                                     aaGAGAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACt                              <  1:519332/150‑1 (MQ=255)
                                                                                                                      agagAACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTa                             >  1:3868420/1‑150 (MQ=255)
                                                                                                                       gagaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTAt                            <  1:467997/150‑1 (MQ=255)
                                                                                                                       gagaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTAt                            >  1:2144165/1‑150 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                           <  2:1499328/150‑1 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                           >  1:4503927/1‑150 (MQ=255)
                                                                                                                        agaACGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATg                           >  1:3934794/1‑150 (MQ=255)
                                                                                                                          accgacggAAATTCCCAATTCCACTAAGTAGGTCCTGAACGGAATCATGATGTCGCAACGCGAAGATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTAGGGc                         <  2:4349295/143‑1 (MQ=255)
                                                                                                                          aaCGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGc                         <  2:3919557/150‑1 (MQ=255)
                                                                                                                           aCGAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCg                        <  2:361063/150‑1 (MQ=255)
                                                                                                                            cGACTGTAATTTCCAATTAGAATAAGTAGTTCCTTATCGGAATAAAGATTTGGCAAAGCTAATATCAATAAGTGCTTAAGGCACCGGATTTCGGTGGTTTAGGAAGATTTGTAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCgg                       <  2:4152684/150‑1 (MQ=255)
                                                                                                                             gAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGt                      >  2:320997/1‑150 (MQ=255)
                                                                                                                             gAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGt                      >  2:2320364/1‑150 (MQ=255)
                                                                                                                             gAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGt                      >  2:5089817/1‑150 (MQ=255)
                                                                                                                             gAATGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGAGTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGt                      >  2:3352556/1‑150 (MQ=255)
                                                                                                                              aaTGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTa                     <  1:644533/150‑1 (MQ=255)
                                                                                                                              aaTGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTa                     >  1:187743/1‑150 (MQ=255)
                                                                                                                               aTGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaa                    <  2:4467928/150‑1 (MQ=255)
                                                                                                                                tGAAATTACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCTCCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTaaa                   >  2:67739/1‑150 (MQ=255)
                                                                                                                                      tACCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAAtt             >  2:1288281/1‑150 (MQ=255)
                                                                                                                                       aCCAATTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTCAAAAAAttt            >  2:3452735/1‑150 (MQ=255)
                                                                                                                                          aaTTAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCa         <  2:405439/150‑1 (MQ=255)
                                                                                                                                            ttAGAATAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATg       >  2:711997/1‑150 (MQ=255)
                                                                                                                                                aaTAAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTACAAAAATTTGCATGGTAt   >  2:2772054/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGCATGGTATT  >  CP009273/2869633‑2869927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑