Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,884,385 G→T 100% V291V (GTC→GTA)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,884,3850GT97.8% 912.8 / 5.8 285V291V (GTC→GTA) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (118/160);  total (122/163)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 285 total aligned reads displayed.

GTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTAAC  >  CP009273/2884239‑2884531
                                                                                                                                                  |                                                                                                                                                  
gtTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACa                                                                                                                                                 <  2:5002401/150‑1 (MQ=255)
    cACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGtt                                                                                                                                             <  1:2823891/150‑1 (MQ=255)
     aCTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTc                                                                                                                                            >  2:4584133/1‑150 (MQ=255)
      cTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCt                                                                                                                                           <  1:2583680/150‑1 (MQ=255)
        gCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCttt                                                                                                                                         >  2:4870987/1‑150 (MQ=255)
            gTAAGCGTGTAGTAATTCTCAACAATGTTGGCGTTGTTGACTGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGATTCATCGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTAAGCCACAGCAGTTATACAAGTTCTTTCAcc                                                                                                                                     <  1:1021121/150‑1 (MQ=255)
             tAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCa                                                                                                                                    <  2:1677239/150‑1 (MQ=255)
             tAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCa                                                                                                                                    >  1:2484125/1‑150 (MQ=255)
                  gTGGCGTAATTCTCAACAATGTGGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGcc                                                                                                                               <  2:75770/150‑1 (MQ=255)
                   tGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCg                                                                                                                              <  1:3270825/150‑1 (MQ=255)
                    ggCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCgg                                                                                                                             >  2:1586757/1‑150 (MQ=255)
                      cGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGAt                                                                                                                           <  2:2432009/150‑1 (MQ=255)
                      cGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGAt                                                                                                                           >  1:4968362/1‑150 (MQ=255)
                          aTTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGtt                                                                                                                       >  1:2015547/1‑150 (MQ=255)
                                aacaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATa                                                                                                                 <  2:3929934/150‑1 (MQ=255)
                                aacaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATa                                                                                                                 >  2:344724/1‑150 (MQ=255)
                                 acaaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATAc                                                                                                                >  2:1493927/1‑150 (MQ=255)
                                  caaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATAcc                                                                                                               <  2:2866170/150‑1 (MQ=255)
                                   aaTGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCa                                                                                                              >  1:1679721/1‑150 (MQ=255)
                                      gtgggCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGAc                                                                                                           <  2:1893435/147‑1 (MQ=255)
                                      gTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGGAGCGCTTCGTTAAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGATCTGATACCAGAc                                                                                                           >  1:2004673/1‑150 (MQ=255)
                                        tGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGc                                                                                                         <  1:932997/150‑1 (MQ=255)
                                          gCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGccc                                                                                                       <  1:2381340/150‑1 (MQ=255)
                                          gCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGccc                                                                                                       <  2:2696362/150‑1 (MQ=255)
                                           cGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCa                                                                                                      <  1:2181254/150‑1 (MQ=255)
                                            ggTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAg                                                                                                     <  1:3825549/150‑1 (MQ=255)
                                            ggTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAg                                                                                                     >  1:4031486/1‑150 (MQ=255)
                                             gtgtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACTGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAgc                                                                                                    <  1:4563919/150‑1 (MQ=255)
                                              tgtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAgcg                                                                                                   <  2:3970783/150‑1 (MQ=255)
                                               gtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAgcgc                                                                                                  <  1:836896/150‑1 (MQ=255)
                                               gtTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAgcgc                                                                                                  <  1:3420579/150‑1 (MQ=255)
                                                  gACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTc                                                                                               >  2:1219902/1‑150 (MQ=255)
                                                    cGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAc                                                                                             <  1:4207035/150‑1 (MQ=255)
                                                     ggTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGAAACGTTTTGCCCTCGACTGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAcc                                                                                            >  1:5146861/1‑150 (MQ=255)
                                                      gTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCAccc                                                                                           <  1:2627235/150‑1 (MQ=255)
                                                       tCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCg                                                                                          <  2:4378209/150‑1 (MQ=255)
                                                       tCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCg                                                                                          <  1:702518/150‑1 (MQ=255)
                                                        cAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCgg                                                                                         >  1:3896617/1‑150 (MQ=255)
                                                           ttCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTg                                                                                      <  1:3856956/150‑1 (MQ=255)
                                                           ttCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTg                                                                                      >  2:4003198/1‑150 (MQ=255)
                                                              gAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTa                                                                                   >  2:4091272/1‑150 (MQ=255)
                                                               aaGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTaa                                                                                  <  1:2707674/150‑1 (MQ=255)
                                                                aGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAAc                                                                                 >  2:138315/1‑150 (MQ=255)
                                                                aGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAAc                                                                                 >  2:3023663/1‑150 (MQ=255)
                                                                    ccACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCAt                                                                             <  1:188064/150‑1 (MQ=255)
                                                                     cACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATg                                                                            <  1:1723472/150‑1 (MQ=255)
                                                                     cACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATg                                                                            <  1:1478574/150‑1 (MQ=255)
                                                                        tGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGccc                                                                         >  1:2893070/1‑150 (MQ=255)
                                                                         gTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCg                                                                        >  2:1255186/1‑150 (MQ=255)
                                                                          gaGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCGTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGa                                                                       <  2:1647276/149‑1 (MQ=255)
                                                                           aGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAg                                                                      <  1:4113915/150‑1 (MQ=255)
                                                                               cTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCAc                                                                  <  2:3156834/150‑1 (MQ=255)
                                                                                 tCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCt                                                                <  2:4815749/150‑1 (MQ=255)
                                                                                  cGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTa                                                               >  2:3363595/1‑150 (MQ=255)
                                                                                   gTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTaa                                                              >  2:2794313/1‑150 (MQ=255)
                                                                                      cAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTc                                                           >  1:4406588/1‑150 (MQ=255)
                                                                                        gagGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCCGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCaa                                                         <  2:2593791/150‑1 (MQ=255)
                                                                                         agGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACACGTTCTTTCTCCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAt                                                        <  2:1824399/150‑1 (MQ=255)
                                                                                          ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAtt                                                       <  2:4028527/150‑1 (MQ=255)
                                                                                          ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAtt                                                       <  2:4239720/150‑1 (MQ=255)
                                                                                          ggCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAta                                                       <  2:2112289/150‑2 (MQ=255)
                                                                                           gCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAAttt                                                      <  2:2323261/150‑1 (MQ=255)
                                                                                            cAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTc                                                     <  2:3095352/150‑1 (MQ=255)
                                                                                               cGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGat                                                  >  1:3990133/1‑150 (MQ=255)
                                                                                                gTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGata                                                 <  1:3133701/150‑1 (MQ=255)
                                                                                                 ttttGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTACCGCATGCCCGAGTCACCTAAGTCAATTTCGatat                                                >  1:4813974/1‑150 (MQ=255)
                                                                                                    tGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGc                                             <  1:4718721/150‑1 (MQ=255)
                                                                                                     gCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCg                                            >  1:2344314/1‑150 (MQ=255)
                                                                                                      cccTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGa                                           >  2:1356825/1‑150 (MQ=255)
                                                                                                       ccTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGaa                                          >  2:4929314/1‑150 (MQ=255)
                                                                                                            aCGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCt                                     >  2:1076221/1‑150 (MQ=255)
                                                                                                             cGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCtt                                    >  1:2841377/1‑150 (MQ=255)
                                                                                                             cGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCtt                                    <  1:4263953/150‑1 (MQ=255)
                                                                                                               gTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCtttt                                  >  1:87806/1‑150 (MQ=255)
                                                                                                                  aCAGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCa                               <  1:1627393/150‑1 (MQ=255)
                                                                                                                    aGGTTCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCaga                             >  2:1258543/1‑150 (MQ=255)
                                                                                                                        tCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTa                         <  1:1789859/150‑1 (MQ=255)
                                                                                                                        tCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTa                         >  2:3330534/1‑150 (MQ=255)
                                                                                                                        tCATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTa                         <  2:561127/150‑1 (MQ=255)
                                                                                                                         cATCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTAc                        >  2:1603715/1‑150 (MQ=255)
                                                                                                                          aTCGCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACg                       <  2:2970434/150‑1 (MQ=255)
                                                                                                                             gCCTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGccc                    >  1:1207863/1‑150 (MQ=255)
                                                                                                                              ccTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCg                   >  2:4646432/1‑150 (MQ=255)
                                                                                                                               cTTTCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCgg                  <  2:3221998/150‑1 (MQ=255)
                                                                                                                                tttCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGt                 <  2:118055/150‑1 (MQ=255)
                                                                                                                                 ttCAGCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTa                >  2:3711440/1‑150 (MQ=255)
                                                                                                                                     gCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAttt            <  2:1023256/150‑1 (MQ=255)
                                                                                                                                     gCCACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAttt            >  1:4674003/1‑150 (MQ=255)
                                                                                                                                      ccACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt           <  2:2414187/150‑1 (MQ=255)
                                                                                                                                      ccACAGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAAtttt           >  1:669271/1‑150 (MQ=255)
                                                                                                                                       cacaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTc          <  1:136358/150‑1 (MQ=255)
                                                                                                                                       cacaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTc          >  2:701965/1‑150 (MQ=255)
                                                                                                                                       cacaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTc          <  1:138315/150‑1 (MQ=255)
                                                                                                                                        acaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCt         <  1:4138964/150‑1 (MQ=255)
                                                                                                                                        acaGCAGTTCTACAAGTTCTTTCACCAGTGCCGGATCGGTCTGAGACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCAGGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCt         <  2:5087040/150‑1 (MQ=255)
                                                                                                                                         cagcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTg        >  1:4694405/1‑150 (MQ=255)
                                                                                                                                           gcagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGt      <  2:3663548/150‑1 (MQ=255)
                                                                                                                                            cagTTCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGtt     <  1:2851441/150‑1 (MQ=255)
                                                                                                                                            cagTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGtt     >  1:305341/1‑150 (MQ=255)
                                                                                                                                               ttCTACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTAAc  <  2:865074/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTTCACTGCGGGTAAGCGTGGCGTAATTCTCAACAATGTTGGCGGTGTTGACGGTCAGTTCGAAGTGCCACTGTAGCGCTTCGTTCAGAGGCAACGTTTTGCCCTCGACGGTGACAGGTTCATCGCCTTTCAGCCACAGCAGTTCGACAAGTTCTTTCACCAGTGCCGGATCGTTCTGATACCAGACGCCCAGCGCGTCACCCGGCTGGTAACGCATGCCCGAGTCACCTAAGTCAATTTCGATATGGCGAACGTCTTTTTCAGAGTTACGCCCGGTAATTTTCTGGTTAAC  >  CP009273/2884239‑2884531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑