Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,915,998 C→A 100% A116S (GCT→TCT)  yqcA ← short‑chain flavodoxin, FMN‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,915,9980CA99.2% 853.4 / ‑2.4 254A116S (GCT→TCT) yqcAshort‑chain flavodoxin, FMN‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (112/140);  total (112/142)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

GATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAATGC  >  CP009273/2915851‑2916144
                                                                                                                                                   |                                                                                                                                                  
gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAgagc                                                                                                                                                  <  1:3001902/150‑1 (MQ=255)
gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAgagc                                                                                                                                                  >  1:5076060/1‑150 (MQ=255)
 attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGct                                                                                                                                                 >  1:3822761/1‑150 (MQ=255)
 attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGct                                                                                                                                                 <  2:2614654/150‑1 (MQ=255)
  ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGctc                                                                                                                                                <  1:4164179/150‑1 (MQ=255)
   tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGctct                                                                                                                                               <  2:4575653/150‑1 (MQ=255)
   tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGctct                                                                                                                                               <  1:5014378/150‑1 (MQ=255)
    cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTg                                                                                                                                              <  2:833810/150‑1 (MQ=255)
    cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTg                                                                                                                                              >  2:4401787/1‑150 (MQ=255)
     aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGt                                                                                                                                             >  1:3588371/1‑150 (MQ=255)
     aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGt                                                                                                                                             >  1:946838/1‑150 (MQ=255)
     aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGt                                                                                                                                             >  2:2978217/1‑150 (MQ=255)
     aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGt                                                                                                                                             >  2:3756333/1‑150 (MQ=255)
       cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTc                                                                                                                                           <  2:1328604/150‑1 (MQ=255)
        aCCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTcc                                                                                                                                          >  2:1388750/1‑150 (MQ=255)
         ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCt                                                                                                                                         >  1:2872185/1‑150 (MQ=255)
         ccGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCt                                                                                                                                         >  1:4444093/1‑150 (MQ=255)
           gCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGc                                                                                                                                       <  1:4667200/150‑1 (MQ=255)
            cTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTTCTGca                                                                                                                                      >  2:2225826/1‑150 (MQ=255)
            cTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGca                                                                                                                                      <  1:4369639/150‑1 (MQ=255)
                aaCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGcaacaa                                                                                                                                  >  1:1628880/1‑150 (MQ=255)
                 aCGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAg                                                                                                                                 <  2:4140338/150‑1 (MQ=255)
                   gACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGc                                                                                                                               <  2:3698328/150‑1 (MQ=255)
                    aCATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCa                                                                                                                              <  1:198697/150‑1 (MQ=255)
                        ttACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGa                                                                                                                          <  1:4674091/150‑1 (MQ=255)
                        ttACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGa                                                                                                                          >  1:3871182/1‑150 (MQ=255)
                        ttACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGa                                                                                                                          >  2:1365922/1‑150 (MQ=255)
                         tACCGCTCGCTGAACAGATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGaa                                                                                                                         <  1:2645928/150‑1 (MQ=255)
                           ccGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACt                                                                                                                       <  2:578384/150‑1 (MQ=255)
                           ccGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACt                                                                                                                       <  1:547733/150‑1 (MQ=255)
                               tcgctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGttt                                                                                                                   >  2:1115075/1‑150 (MQ=255)
                                 gctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTgc                                                                                                                 >  1:1799250/1‑150 (MQ=255)
                                  ctGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTgcc                                                                                                                >  2:3185220/1‑150 (MQ=255)
                                     aaCATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTgccgcc                                                                                                             <  2:572099/150‑1 (MQ=255)
                                      aCATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCa                                                                                                            <  2:1764662/150‑1 (MQ=255)
                                      aCATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCa                                                                                                            <  2:4252614/150‑1 (MQ=255)
                                        atatCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCAtt                                                                                                          <  1:3756333/150‑1 (MQ=255)
                                         tatCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTg                                                                                                         <  1:3698170/150‑1 (MQ=255)
                                           tCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCa                                                                                                       <  2:3420817/150‑1 (MQ=255)
                                           tCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCa                                                                                                       <  2:1204849/150‑1 (MQ=255)
                                            cAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAg                                                                                                      <  2:4341688/150‑1 (MQ=255)
                                              ggACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGaa                                                                                                    <  1:3428502/150‑1 (MQ=255)
                                                  aacaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTc                                                                                                <  2:1600315/150‑1 (MQ=255)
                                                   acaGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTca                                                                                               >  1:2594055/1‑150 (MQ=255)
                                                     aGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTAGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCCTTGCAGAAATTcaca                                                                                             >  1:4116945/1‑150 (MQ=255)
                                                      gCGTGCCCCACTGTTCGACCCACGGATTTGTTTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACAt                                                                                            <  1:2156466/150‑1 (MQ=255)
                                                        gTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATaa                                                                                          <  2:1982600/150‑1 (MQ=255)
                                                           ccccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAacta                                                                                       >  1:205059/1‑150 (MQ=255)
                                                            cccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAactac                                                                                      <  1:4145604/150‑1 (MQ=255)
                                                             ccACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAactact                                                                                     <  2:2035579/150‑1 (MQ=255)
                                                                cTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTc                                                                                  <  2:1483931/150‑1 (MQ=255)
                                                                 tGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCg                                                                                 <  1:5110196/150‑1 (MQ=255)
                                                                 tGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCg                                                                                 >  2:2305447/1‑150 (MQ=255)
                                                                    tCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCg                                                                              <  1:3492862/150‑1 (MQ=255)
                                                                     cGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGa                                                                             <  1:1518049/150‑1 (MQ=255)
                                                                     cGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGa                                                                             >  2:4620100/1‑150 (MQ=255)
                                                                      gACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAg                                                                            >  1:3260041/1‑150 (MQ=255)
                                                                        cccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAgcg                                                                          <  2:283907/150‑1 (MQ=255)
                                                                         ccACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAgcgc                                                                         <  2:2830190/150‑1 (MQ=255)
                                                                            cGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAAt                                                                      <  1:2367014/150‑1 (MQ=255)
                                                                             ggATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATc                                                                     >  2:4175469/1‑150 (MQ=255)
                                                                               aTTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCAc                                                                   >  2:2051459/1‑150 (MQ=255)
                                                                                    aTTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCAt                                                              >  2:4782621/1‑150 (MQ=255)
                                                                                      tCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATaa                                                            <  1:1043549/150‑1 (MQ=255)
                                                                                          tttCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCa                                                        <  2:3338115/150‑1 (MQ=255)
                                                                                            tccggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGa                                                      >  1:1150432/1‑150 (MQ=255)
                                                                                             ccggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGAt                                                     <  1:1291878/150‑1 (MQ=255)
                                                                                               ggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTc                                                   <  2:1945134/150‑1 (MQ=255)
                                                                                               ggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTc                                                   >  1:1916450/1‑150 (MQ=255)
                                                                                               ggttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTc                                                   <  2:3000142/150‑1 (MQ=255)
                                                                                                 ttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCgg                                                 <  1:312903/150‑1 (MQ=255)
                                                                                                 ttccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCgg                                                 >  1:1561774/1‑150 (MQ=255)
                                                                                                  tccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGc                                                >  2:4751628/1‑150 (MQ=255)
                                                                                                   ccggGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCt                                               <  1:2712170/150‑1 (MQ=255)
                                                                                                     gggTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTgg                                             <  1:3365224/150‑1 (MQ=255)
                                                                                                        ttCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAg                                          <  1:3178858/150‑1 (MQ=255)
                                                                                                         tctcACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGc                                         >  1:3368034/1‑150 (MQ=255)
                                                                                                         tctcACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGc                                         >  2:4439933/1‑150 (MQ=255)
                                                                                                                ggCGTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACt                                  <  1:4132605/150‑1 (MQ=255)
                                                                                                                  cGTCACTCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGGCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTAt                                <  2:5077596/150‑1 (MQ=255)
                                                                                                                   gTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATc                               <  2:4110420/150‑1 (MQ=255)
                                                                                                                   gTCAATCAACAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATc                               <  1:221206/150‑1 (MQ=255)
                                                                                                                    tcaatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCt                              <  2:4653088/150‑1 (MQ=255)
                                                                                                                     caatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCtt                             >  2:2132750/1‑150 (MQ=255)
                                                                                                                      aatcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCCTTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCttt                            >  2:2709870/1‑150 (MQ=255)
                                                                                                                       atcaaCAGCATTTCGCCAACCCGCTGAGAGCTCTGTTTCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTg                           <  1:2225891/150‑1 (MQ=255)
                                                                                                                            cAGCATTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTcc                      >  1:807460/1‑150 (MQ=255)
                                                                                                                                aTTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGa                  >  1:1255330/1‑150 (MQ=255)
                                                                                                                                aTTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGa                  <  1:3965400/150‑1 (MQ=255)
                                                                                                                                aTTTCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGa                  <  2:4035948/150‑1 (MQ=255)
                                                                                                                                   tCGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAag               >  1:1737767/1‑150 (MQ=255)
                                                                                                                                    cGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAaga              >  2:3545917/1‑150 (MQ=255)
                                                                                                                                    cGCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAaga              <  1:2073812/150‑1 (MQ=255)
                                                                                                                                     gCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAagag             >  2:3270287/1‑150 (MQ=255)
                                                                                                                                     gCCAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAagag             >  1:4884915/1‑150 (MQ=255)
                                                                                                                                      ccAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGt            <  2:3822761/150‑1 (MQ=255)
                                                                                                                                       cAACCCGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTg           >  1:4465698/1‑150 (MQ=255)
                                                                                                                                           ccGCTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGcac       >  2:2602091/1‑150 (MQ=255)
                                                                                                                                              cTGAGAGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAAt    <  1:1588107/150‑1 (MQ=255)
                                                                                                                                                gagaGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAATGc  >  1:4176435/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGGACAACAGCGTGCCCCACTGTTCGACCCACGGATTTGATTCCGTTTCCGGTTCCGGGTTCTCACTGGCGTCAATCAACAGCATTTCGCCAACCCGCTGAGCGCTCTGTTCCTGCAACAAGGCATCGAACTGTTTGCCGCCATTGCAGAAATTCACATAACTACTGTCGCCGAGCGCAATCACGCCATAACGCAGATTCGGCTGGAAGCCCAGACTATCTTTGATTCCCTGAAAGAGTGGCACAATGC  >  CP009273/2915851‑2916144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑