Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,920,050 C→A 100% I128I (ATC→ATA)  ygdH → UPF0717 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,920,0500CA96.3% 892.4 / 20.3 298I128I (ATC→ATA) ygdHUPF0717 family protein
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base A (123/164);  total (126/172)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 299 total aligned reads displayed.

CCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCG  >  CP009273/2919904‑2920196
                                                                                                                                                  |                                                                                                                                                  
ccGGAAGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACt                                                                                                                                                 <  2:2913763/150‑1 (MQ=255)
ccGGAAGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACt                                                                                                                                                 <  2:1899824/150‑1 (MQ=255)
acGGAAGAGGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCTGGACAACTTGTTAGCGGTCCTGCTTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACt                                                                                                                                                 <  1:4455981/149‑1 (MQ=255)
   gAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAAc                                                                                                                                              <  2:224021/150‑1 (MQ=255)
      gagGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTg                                                                                                                                           >  2:4890231/1‑150 (MQ=255)
       agGCTTTTGTCTATGGGCGAATTATTCTCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTgg                                                                                                                                          <  2:682273/150‑1 (MQ=255)
       agGCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTgg                                                                                                                                          >  2:1978023/1‑150 (MQ=255)
        ggCTTTTGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGt                                                                                                                                         <  1:2867168/150‑1 (MQ=255)
           ttttGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCtt                                                                                                                                      >  1:1379009/1‑150 (MQ=255)
            tttGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCttt                                                                                                                                     >  2:1865289/1‑150 (MQ=255)
              tGTCTATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTc                                                                                                                                   <  1:5125869/150‑1 (MQ=255)
              tGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTc                                                                                                                                   >  2:574019/1‑150 (MQ=255)
               gTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTcc                                                                                                                                  <  1:4127854/150‑1 (MQ=255)
                tctaTGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCCGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCa                                                                                                                                 <  2:151156/147‑1 (MQ=255)
                 ctaagggCGAATTATTCGCGCTTTTCAGCCCCACTTGTGCGCGGTCCTGCGTGACATTCTCTACGTTTCCGCGAAAATACATAACAACGTGCGTTTTCCCAACCTGAATCACGACAACTCAGTCCACATAACTAAACTGGTCTATTACAt                                                                                                                                >  2:5084492/5‑150 (MQ=255)
                    tGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCtt                                                                                                                             >  1:1433575/1‑150 (MQ=255)
                      ggCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGc                                                                                                                           >  2:3168719/1‑150 (MQ=255)
                       gCGAATTAGTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCg                                                                                                                          <  1:4421237/150‑1 (MQ=255)
                        cGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGt                                                                                                                         <  2:5140637/150‑1 (MQ=255)
                         gAATTATTCGCGCTTTGCAGGTCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTa                                                                                                                        >  2:2092478/1‑150 (MQ=255)
                         gAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTa                                                                                                                        >  2:418535/1‑150 (MQ=255)
                           attattCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAAc                                                                                                                      <  1:826790/150‑1 (MQ=255)
                            ttattCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACg                                                                                                                     <  1:4059182/150‑1 (MQ=255)
                              attCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCt                                                                                                                   <  2:4127595/150‑1 (MQ=255)
                                tCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcg                                                                                                                 <  1:2114181/150‑1 (MQ=255)
                                 cgcgCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgc                                                                                                                >  1:1996391/1‑150 (MQ=255)
                                  gcgcATAGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAGTCTCTTCGTTGACGGGCAACTCCATAACACCGTTCGTTGTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgcg                                                                                                               <  2:4395400/150‑1 (MQ=255)
                                   cgcTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgcgc                                                                                                              <  1:3392721/150‑1 (MQ=255)
                                   cgcTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgcgc                                                                                                              >  2:1505945/1‑150 (MQ=255)
                                    gcTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTcgcgcg                                                                                                             <  1:1195368/150‑1 (MQ=255)
                                      tttGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCt                                                                                                           <  2:4288774/150‑1 (MQ=255)
                                        tGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGc                                                                                                         <  2:101914/150‑1 (MQ=255)
                                              ccAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTgg                                                                                                   <  1:3118742/150‑1 (MQ=255)
                                               cAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTggg                                                                                                  <  1:1324049/150‑1 (MQ=255)
                                                aaCTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGt                                                                                                 <  2:2443852/150‑1 (MQ=255)
                                                    tGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAg                                                                                             <  2:2577383/150‑1 (MQ=255)
                                                      ttCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAgcg                                                                                           <  2:3276631/150‑1 (MQ=255)
                                                        cgcgGCCCTGCGTGACACTCTCTTCGTCTACGGGCAAATCCATACCACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGcc                                                                                         <  1:4819501/150‑1 (MQ=255)
                                                           ggTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCaaa                                                                                      >  1:2311675/1‑150 (MQ=255)
                                                              ccTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAatat                                                                                   <  2:3141222/150‑1 (MQ=255)
                                                              ccTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAatat                                                                                   >  2:1769289/1‑150 (MQ=255)
                                                                tGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtgg                                                                                 >  2:3369098/1‑150 (MQ=255)
                                                                tGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtgg                                                                                 <  2:4315552/150‑1 (MQ=255)
                                                                 gCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtggt                                                                                <  1:3029530/150‑1 (MQ=255)
                                                                     gACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTc                                                                            <  1:4606639/150‑1 (MQ=255)
                                                                     gACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTc                                                                            >  1:3781812/1‑150 (MQ=255)
                                                                        aTTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTctgc                                                                         >  2:2116310/1‑150 (MQ=255)
                                                                            tctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTggg                                                                     <  1:2989765/150‑1 (MQ=255)
                                                                             ctTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTgggg                                                                    >  1:1156825/1‑150 (MQ=255)
                                                                               tCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCg                                                                  >  1:1113781/1‑150 (MQ=255)
                                                                               tCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCg                                                                  <  2:3632572/150‑1 (MQ=255)
                                                                                   ttACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCa                                                              <  2:843964/150‑1 (MQ=255)
                                                                                    tACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCAc                                                             >  1:92847/1‑150 (MQ=255)
                                                                                     aCGGGCAAATCCATAACACCGTTCGTTGTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACt                                                            <  2:4784956/150‑1 (MQ=255)
                                                                                       gggCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCa                                                          >  1:627896/1‑150 (MQ=255)
                                                                                       gggCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCa                                                          <  1:1256283/150‑1 (MQ=255)
                                                                                          cAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAtt                                                       >  2:3889571/1‑150 (MQ=255)
                                                                                           aaaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTa                                                      >  1:3271599/1‑150 (MQ=255)
                                                                                            aaTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTaa                                                     >  1:3691490/1‑150 (MQ=255)
                                                                                             aTCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAAc                                                    <  2:1269694/150‑1 (MQ=255)
                                                                                                cATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTCACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaa                                                 <  2:4822356/150‑1 (MQ=255)
                                                                                                cATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGaa                                                 >  1:2568508/1‑150 (MQ=255)
                                                                                                cATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAAGTAACGaa                                                 >  2:3054388/1‑150 (MQ=255)
                                                                                                   aaCACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAAc                                              >  1:549848/1‑150 (MQ=255)
                                                                                                     cacCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGa                                            >  2:4671079/1‑150 (MQ=255)
                                                                                                        cgttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTa                                         >  2:3957484/1‑150 (MQ=255)
                                                                                                         gttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTAt                                        >  2:2653395/1‑150 (MQ=255)
                                                                                                          ttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTAtt                                       <  1:3349732/150‑1 (MQ=255)
                                                                                                            cgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTg                                     >  1:4118981/1‑150 (MQ=255)
                                                                                                            cgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTg                                     <  1:4173858/150‑1 (MQ=255)
                                                                                                            cgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTg                                     <  1:264794/150‑1 (MQ=255)
                                                                                                             gttTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGt                                    >  1:489840/1‑150 (MQ=255)
                                                                                                               tttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTAt                                  >  2:283166/1‑150 (MQ=255)
                                                                                                                ttCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATg                                 <  1:2817177/150‑1 (MQ=255)
                                                                                                                 tCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGc                                >  1:3961166/1‑150 (MQ=255)
                                                                                                                 tCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGc                                >  2:1550791/1‑150 (MQ=255)
                                                                                                                  cccAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGcc                               <  1:4961600/150‑1 (MQ=255)
                                                                                                                    cAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATCTGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcg                             >  1:4748399/1‑150 (MQ=255)
                                                                                                                     aaCCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgt                            >  2:821836/1‑150 (MQ=255)
                                                                                                                        cTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgc                         <  2:3233737/150‑1 (MQ=255)
                                                                                                                        cTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgc                         <  1:1671403/150‑1 (MQ=255)
                                                                                                                         tGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcg                        <  1:1869897/150‑1 (MQ=255)
                                                                                                                          gAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgt                       >  2:3074138/1‑150 (MQ=255)
                                                                                                                           aaTCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgtc                      <  2:4432199/150‑1 (MQ=255)
                                                                                                                            aTCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgtcg                     >  1:333382/1‑150 (MQ=255)
                                                                                                                            aTCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgtcg                     >  1:3883476/1‑150 (MQ=255)
                                                                                                                              ctcGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGa                   <  1:78457/150‑1 (MQ=255)
                                                                                                                              ctcGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGa                   <  1:2535151/150‑1 (MQ=255)
                                                                                                                                cGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaaa                 >  1:3335780/1‑150 (MQ=255)
                                                                                                                                  aCAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAAcc               <  2:4956944/150‑1 (MQ=255)
                                                                                                                                      cTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt           >  1:2548074/1‑150 (MQ=255)
                                                                                                                                       tCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTg          >  1:3460101/1‑150 (MQ=255)
                                                                                                                                        ccGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTgg         <  1:238265/150‑1 (MQ=255)
                                                                                                                                        ccGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTgg         <  1:1393847/150‑1 (MQ=255)
                                                                                                                                            ccACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCt     <  2:2470896/150‑1 (MQ=255)
                                                                                                                                            ccACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCt     <  2:4820934/150‑1 (MQ=255)
                                                                                                                                            ccACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCt     <  2:5027378/150‑1 (MQ=255)
                                                                                                                                              acaTAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGc   >  1:3188316/1‑150 (MQ=255)
                                                                                                                                              acaTAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGATCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGc   >  2:2298100/1‑150 (MQ=255)
                                                                                                                                               caTAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCg  <  1:4240811/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCTGGGCCTGCG  >  CP009273/2919904‑2920196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑