Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,934,843 G→T 100% H50N (CAT→AAT)  ygdD ← UPF0382 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,934,8430GT99.6% 889.9 / ‑4.3 267H50N (CAT→AAT) ygdDUPF0382 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (134/131);  total (136/131)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 267 total aligned reads displayed.

CCCACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCA  >  CP009273/2934696‑2934989
                                                                                                                                                   |                                                                                                                                                  
cccACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTaa                                                                                                                                                  >  2:493510/1‑150 (MQ=255)
   acaAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACg                                                                                                                                               <  2:3828394/150‑1 (MQ=255)
     aaaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGcc                                                                                                                                             <  2:4202311/150‑1 (MQ=255)
     aaaCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGcc                                                                                                                                             <  1:1898951/150‑1 (MQ=255)
       aCGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTg                                                                                                                                           <  2:4109255/150‑1 (MQ=255)
        cGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTgg                                                                                                                                          <  1:2484783/150‑1 (MQ=255)
          cAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTa                                                                                                                                        >  1:2231690/1‑150 (MQ=255)
          cAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGAGACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTa                                                                                                                                        <  2:776453/150‑1 (MQ=255)
           aGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGtct                                                                                                                                       >  2:2662637/1‑148 (MQ=255)
           aGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTAt                                                                                                                                       <  1:3728684/150‑1 (MQ=255)
            gATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTAtt                                                                                                                                      >  1:637506/1‑150 (MQ=255)
               gggACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGa                                                                                                                                   <  2:1220803/150‑1 (MQ=255)
                ggACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTTCCTAACGCGAGTAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGATGCATTGCCACCGCCAGACCTAAGATCGCCAGCTTATTAAACGCCTGGTATTCGAg                                                                                                                                  <  1:1378387/150‑1 (MQ=255)
                 gACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTAGTCGAgg                                                                                                                                 >  2:3472529/1‑150 (MQ=255)
                   cAGCGCCAGTCAATAAAGTCTGCCTCTGAACAACACCGTGCCTAACGCTAGGAAAACGCTACTCCTGTTAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGcc                                                                                                                               <  2:589403/150‑1 (MQ=255)
                    aGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAACCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCCGCGTATTAAACGCCTGGTCGTCGAGGCCg                                                                                                                              >  2:1994579/1‑150 (MQ=255)
                    aGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCg                                                                                                                              >  1:1049235/1‑150 (MQ=255)
                       gcCAGGCAATAAAGGCTGTCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTc                                                                                                                           >  2:1132756/1‑150 (MQ=255)
                       gcCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTc                                                                                                                           <  2:4971764/150‑1 (MQ=255)
                        ccAGGCAATCAAGGCTGCCGCTGAACACCACCGTGCCTACCGCGAGGAAAACGCTACTCCCGTAACACCAGAGACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCt                                                                                                                          <  2:4385872/150‑1 (MQ=255)
                        ccAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCt                                                                                                                          >  2:3561785/1‑150 (MQ=255)
                        ccAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCt                                                                                                                          <  1:4991614/150‑1 (MQ=255)
                         cAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTg                                                                                                                         >  1:3249543/1‑150 (MQ=255)
                          aGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTgg                                                                                                                        >  1:1608724/1‑150 (MQ=255)
                            gCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGAt                                                                                                                      >  1:3199398/1‑150 (MQ=255)
                              aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                    >  1:3330802/1‑150 (MQ=255)
                              aaTAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATcc                                                                                                                    <  2:2592992/150‑1 (MQ=255)
                                      cTGCCGCTGAACAACACCTTGCCTAACGCGATGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtc                                                                                                            <  2:2184610/150‑1 (MQ=255)
                                       tGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                           >  2:1502967/1‑150 (MQ=255)
                                       tGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                           >  1:4900114/1‑150 (MQ=255)
                                       tGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtct                                                                                                           <  2:717706/150‑1 (MQ=255)
                                        gCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtctc                                                                                                          <  1:1706206/150‑1 (MQ=255)
                                        gCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCAtctc                                                                                                          <  1:4044152/150‑1 (MQ=255)
                                         ccGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCa                                                                                                         >  1:2144153/1‑150 (MQ=255)
                                         ccGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCa                                                                                                         <  1:1934283/150‑1 (MQ=255)
                                          cGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCaa                                                                                                        <  2:4655669/150‑1 (MQ=255)
                                            cTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACg                                                                                                      <  2:2539006/150‑1 (MQ=255)
                                             tGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACgg                                                                                                     >  1:1542993/1‑150 (MQ=255)
                                               aacaacACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcc                                                                                                   >  2:3689541/1‑150 (MQ=255)
                                               aacaacACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcc                                                                                                   >  2:1309320/1‑150 (MQ=255)
                                                 caacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGcccc                                                                                                 >  2:363443/1‑150 (MQ=255)
                                                  aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGTATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                <  1:2758571/150‑1 (MQ=255)
                                                  aacaCCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGccccc                                                                                                >  1:4815296/1‑150 (MQ=255)
                                                   acacCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCa                                                                                               <  2:637506/150‑1 (MQ=255)
                                                     acCGTGCCTTACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                             <  1:4227060/150‑1 (MQ=255)
                                                     acCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATg                                                                                             >  2:1092083/1‑150 (MQ=255)
                                                        gTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGtt                                                                                          <  2:2304858/150‑1 (MQ=255)
                                                          gCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGtttt                                                                                        >  2:925764/1‑150 (MQ=255)
                                                            cTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTAc                                                                                      <  1:5022390/150‑1 (MQ=255)
                                                                  tgAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAaca                                                                                >  2:3348922/2‑150 (MQ=255)
                                                                   gAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAacac                                                                               >  1:4744036/1‑150 (MQ=255)
                                                                      gAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATg                                                                            <  1:5102646/150‑1 (MQ=255)
                                                                       aaaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgc                                                                           >  1:754546/1‑150 (MQ=255)
                                                                       aaaaCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgc                                                                           <  1:1959110/150‑1 (MQ=255)
                                                                          aCGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATgcgcg                                                                        >  1:2933574/1‑150 (MQ=255)
                                                                             cTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCa                                                                     >  1:2795148/1‑150 (MQ=255)
                                                                             cTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCa                                                                     >  1:186074/1‑150 (MQ=255)
                                                                               aCTCCAGTAAAACCAGATACTGATGCGACGCTGCATTACCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCaaa                                                                   >  1:1526329/1‑150 (MQ=255)
                                                                                 tCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAg                                                                 >  2:4434190/1‑150 (MQ=255)
                                                                                 tCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAg                                                                 >  1:1361148/1‑150 (MQ=255)
                                                                                 tCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTATGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAg                                                                 <  1:5088260/150‑1 (MQ=255)
                                                                                         aaCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCaga                                                         <  1:1149983/150‑1 (MQ=255)
                                                                                          aCCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCagag                                                        <  2:4828544/150‑1 (MQ=255)
                                                                                              gATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcac                                                    >  1:960657/1‑150 (MQ=255)
                                                                                               aTACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCcaca                                                   <  1:1742158/150‑1 (MQ=255)
                                                                                                 aCTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaa                                                 >  1:3534747/1‑150 (MQ=255)
                                                                                                 aCTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaa                                                 >  2:3515963/1‑150 (MQ=255)
                                                                                                 aCTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaa                                                 <  2:2352275/150‑1 (MQ=255)
                                                                                                   tGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACaaaaa                                               >  2:4140867/1‑150 (MQ=255)
                                                                                                     aTGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATg                                             <  1:4557226/150‑1 (MQ=255)
                                                                                                      tGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGa                                            >  2:4073951/1‑150 (MQ=255)
                                                                                                        cGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAg                                          >  2:1483461/1‑150 (MQ=255)
                                                                                                        cGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAg                                          >  2:2950063/1‑150 (MQ=255)
                                                                                                           cGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCg                                       >  1:2201141/1‑150 (MQ=255)
                                                                                                              tGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTa                                    <  1:79252/150‑1 (MQ=255)
                                                                                                              tGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTa                                    <  2:4583645/150‑1 (MQ=255)
                                                                                                               gCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAACGCGCCCAGAGCCACAAAAATGAAGCCGCTaa                                   <  2:3999782/150‑1 (MQ=255)
                                                                                                               gCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTaa                                   >  2:3249502/1‑150 (MQ=255)
                                                                                                                  ttGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATgg                                >  2:1793805/1‑150 (MQ=255)
                                                                                                                   tGCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggc                               <  2:5135572/150‑1 (MQ=255)
                                                                                                                    gCCACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcg                              <  2:3447518/150‑1 (MQ=255)
                                                                                                                     ccACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcgg                             <  1:2569604/150‑1 (MQ=255)
                                                                                                                      cACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcggc                            >  1:5130087/1‑150 (MQ=255)
                                                                                                                      cACCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATggcggc                            <  2:4485364/150‑1 (MQ=255)
                                                                                                                       aCCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGgcggcg                           <  1:99305/150‑1 (MQ=255)
                                                                                                                       aCCGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGgcggcg                           <  1:4395138/150‑1 (MQ=255)
                                                                                                                       aCCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGgcggcg                           >  1:3370373/1‑150 (MQ=255)
                                                                                                                        ccGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGa                          >  1:100717/1‑150 (MQ=255)
                                                                                                                         cGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAACGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaa                         <  2:2689953/150‑1 (MQ=255)
                                                                                                                         cGCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaa                         <  1:2452321/150‑1 (MQ=255)
                                                                                                                          gCCAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaaa                        >  2:3139750/1‑150 (MQ=255)
                                                                                                                           ccAGACCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGaaaa                       <  2:4207042/150‑1 (MQ=255)
                                                                                                                               aCCTAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAg                   >  1:5041756/1‑150 (MQ=255)
                                                                                                                                  tAAGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCAt                <  2:2740528/150‑1 (MQ=255)
                                                                                                                                    aGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATaa              <  2:3956583/150‑1 (MQ=255)
                                                                                                                                    aGATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATaa              >  1:4436785/1‑150 (MQ=255)
                                                                                                                                     gATCGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATaaa             >  1:1989463/1‑150 (MQ=255)
                                                                                                                                        cGCCAGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCAAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACg          <  2:4754485/150‑1 (MQ=255)
                                                                                                                                            aGCGTATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTg      >  1:3742367/1‑150 (MQ=255)
                                                                                                                                                tATTAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCa  <  2:2973718/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCACAAACGCAGATGGGACAGCGCCAGGCAATAAAGGCTGCCGCTGAACAACACCGTGCCTAACGCGAGGAAAACGCTACTCCAGTAAAACCAGATACTGATGCGACGCTGCATTGCCACCGCCAGACCTAAGATCGCCAGCGTATGAAACGCCTGGTATTCGAGGCCGGTCTGGATCCAGCCCATCTCAACGGCCCCCATGGTTTTACTTAACACATGCGCGCCAAAAGCGCCCAGAGCCACAAAAATGAAGCCGCTAATGGCGGCGAAAATCAGCATAAAACGGCTGGTCA  >  CP009273/2934696‑2934989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑